View source for pTYB11

==At a Glance ==
[[Image:PTYB11.jpg|thumb|500px|right|pTYB11; Image provided by [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/pTYB11_map.pdf NEB].]]<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.X46e17bf5de3b8-->
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!align=left {{table heading style}} |Type
||
Cloning & Expression
|-
!align=left {{table heading style}} |Origin of Replication
||
pMB1 <br /> 
M13
|-
!align=left {{table heading style}} |Copy #
||
~20 per cell
|-
!align=left {{table heading style}} |Markers
||
Ampicillin
|-
!align=left {{table heading style}} |Link to Sequence
||
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/ptyb11.txt NEB]
|-
!align=left {{table heading style}} |Features
||
lacI 
<ref name='PMID:1902522'/> <br />
T7 promoter 
<ref name='PMID:1902522'/> <br /> 
expression ORF: <br />
Sce VMA intein 
<ref name='PMID:9801307'/>
<ref name='PMID:9224900'/>
<ref name='PMID:9553117'/> <br />
MCS <br />
CBD
<ref name='PMID:9801307'/>
<ref name='PMID:9224900'/> 

|-
!align=left {{table heading style}} |Accession Number
||


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==Notes==
The pTYB1 is an E. coli vector created for fusion protein expression and purification. A target gene can be inserted into the the ORF by use of the MCS downstream of the Sce VMA intein. The SapI site in the MCS is responsible for cloning the 5' end of the inserted gene. The chitin-binding domain(CBD) within the intein gene results in a intein-protein with chitin beads that facilitate protein purification. Release of the protein is induced by the thiol cleavage activity of the intein.

==History ==
pTYB11 is derived from E. coli plamid pBR322, its chitin-binding domain(CBD) is isolated from B. circulans, and the VMA intein from S. cerevisiae.

==Sources ==
[http://www.neb.com/nebecomm/products/productN6901.asp New England Biolabs]

==References==
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<references />

==Sequence ==
       1  AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTTT 
      51  GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 
     101  CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA 
     151  ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 
     201  TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG 
     251  TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT 
     301  CCTTGAGAGT TTTCGCCCCG AAGAACGTTC TCCAATGATG AGCACTTTTA 
     351  AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG 
     401  CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC 
     451  ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 
     501  GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG 
     551  ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG 
     601  GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA 
     651  TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 
     701  TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA 
     751  ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 
     801  CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG 
     851  CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC 
     901  CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC 
     951  GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 
    1001  CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TACCCCGGTT 
    1051  GATAATCAGA AAAGCCCCAA AAACAGGAAG ATTGTATAAG CAAATATTTA 
    1101  AATTGTAAAC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA 
    1151  TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA 
    1201  TCAAAAGAAT AGCCCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA 
    1251  GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG 
    1301  TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCAA ATCAAGTTTT 
    1351  TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC 
    1401  CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG 
    1451  GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC 
    1501  ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG 
    1551  CGCGTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 
    1601  ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 
    1651  GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 
    1701  TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA 
    1751  GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 
    1801  ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 
    1851  ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 
    1901  GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 
    1951  ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 
    2001  CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG 
    2051  CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 
    2101  GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 
    2151  CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 
    2201  TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 
    2251  GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 
    2301  CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC 
    2351  CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC 
    2401  CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC TATGGTGCAC TCTCAGTACA 
    2451  ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA 
    2501  CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC 
    2551  GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA 
    2601  CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA 
    2651  CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GCAGCGATTC 
    2701  ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA 
    2751  GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT 
    2801  TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG 
    2851  GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG 
    2901  ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG 
    2951  GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCC 
    3001  GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA 
    3051  TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT 
    3101  TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT 
    3151  CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG 
    3201  CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG 
    3251  GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA 
    3301  GGCTCTCAAG GGCATCGGTC GAGATCCCGG TGCCTAATGA GTGAGCTAAC 
    3351  TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 
    3401  TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 
    3451  GCGTATTGGG CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC 
    3501  AGCTGATTGC CCTTCACCGC CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC 
    3551  CACGCTGGTT TGCCCCAGCA GGCGAAAATC CTGTTTGATG GTGGTTAACG 
    3601  GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC CACTACCGAG 
    3651  ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC 
    3701  CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT 
    3751  CATTCAGCAT TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG 
    3801  CCTTCCCGTT CCGCTATCGG CTGAATTTGA TTGCGAGTGA GATATTTATG 
    3851  CCAGCCAGCC AGACGCAGAC GCGCCGAGAC AGAACTTAAT GGGCCCGCTA 
    3901  ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC CACGCCCAGT 
    3951  CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC 
    4001  AGAGACATCA AGAAATAACG CCGGAACATT AGTGCAGGCA GCTTCCACAG 
    4051  CAATGGCATC CTGGTCATCC AGCGGATAGT TAATGATCAG CCCACTGACG 
    4101  CGTTGCGCGA GAAGATTGTG CACCGCCGCT TTACAGGCTT CGACGCCGCT 
    4151  TCGTTCTACC ATCGACACCA CCACGCTGGC ACCCAGTTGA TCGGCGCGAG 
    4201  ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC CAGACTGGAG 
    4251  GTGGCAACGC CAATCAGCAA CGACTGTTTG CCCGCCAGTT GTTGTGCCAC 
    4301  GCGGTTGGGA ATGTAATTCA GCTCCGCCAT CGCCGCTTCC ACTTTTTCCC 
    4351  GCGTTTTCGC AGAAACGTGG CTGGCCTGGT TCACCACGCG GGAAACGGTC 
    4401  TGATAAGAGA CACCGGCATA CTCTGCGACA TCGTATAACG TTACTGGTTT 
    4451  CACATTCACC ACCCTGAATT GACTCTCTTC CGGGCGCTAT CATGCCATAC 
    4501  CGCGAAAGGT TTTGCGCCAT TCGATGGTGT CCGGGATCTC GACGCTCTCC 
    4551  CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT 
    4601  TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC 
    4651  AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT 
    4701  CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA 
    4751  TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG 
    4801  CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAATT AATACGACTC 
    4851  ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCTCTAG AAATAATTTT 
    4901  GTTTAACTTT AAGAAGGAGA TATACTTATG AAAATCGAAG AAGGTAAACT 
    4951  GGTAATCGGT TCCCTGGAGG GTTGCTTTGC CAAGGGTACC AATGTTTTAA 
    5001  TGGCGGATGG GTCTATTGAA TGTATTGAAA ACATTGAGGT TGGTAATAAG 
    5051  GTCATGGGTA AAGATGGCAG ACCTCGTGAG GTAATTAAAT TGCCCAGAGG 
    5101  AAGAGAAACT ATGTACAGCG TCGTGCAGAA AAGTCAGCAC AGAGCCCACA 
    5151  AAAGTGACTC AAGTCGTGAA GTGCCAGAAT TACTCAAGTT TACGTGTAAT 
    5201  GCGACCCATG AGTTGGTTGT TAGAACACCT CGTAGTGTCC GCCGTTTGTC 
    5251  TCGTACCATT AAGGGTGTCG AATATTTTGA AGTTATTACT TTTGAGATGG 
    5301  GCCAAAAGAA AGCCCCCGAC GGTAGAATTG TTGAGCTTGT CAAGGAAGTT 
    5351  TCAAAGAGCT ACCCAATATC TGAGGGGCCT GAGAGAGCCA ACGAATTAGT 
    5401  AGAATCCTAT AGAAAGGCTT CAAATAAAGC TTATTTTGAG TGGACTATTG 
    5451  AGGCCAGAGA TCTTTCTCTG TTGGGTTCCC ATGTTCGTAA AGCTACCTAC 
    5501  CAGACTTACG CTCCAATTCT TTATGAGAAT GACCACTTTT TCGACTACAT 
    5551  GCAAAAAAGT AAGTTTCATC TCACCATTGA AGGTCCAAAA GTACTTGCTT 
    5601  ATTTACTTGG TTTATGGATT GGTGATGGAT TGTCTGACAG GGCAACTTTT 
    5651  TCGGTTGATT CCAGAGATAC TTCTTTGATG GAACGTGTTA CTGAATATGC 
    5701  TGAAAAGTTG AATTTGTGCG CCGAGTATAA GGACAGAAAA GAACCACAAG 
    5751  TTGCCAAAAC TGTTAATTTG TACTCTAAAG TTGTCAGAGG TGCTAGCACA 
    5801  AATCCTGGTG TATCCGCTTG GCAGGTCAAC ACAGCTTATA CTGCGGGACA 
    5851  ATTGGTCACA TATAACGGCA AGACGTATAA ATGTTTGCAG CCCCACACCT 
    5901  CCTTGGCAGG ATGGGAACCA TCCAACGTTC CTGCCTTGTG GCAGCTTCAA 
    5951  GGTGGCCATG GTGGTATTCG CAATAATCTT AATACTGAGA ATCCATTATG 
    6001  GGACGCTATT GTTGGCTTAG GATTCTTGAA GGACGGTGTC AAAAATATTC 
    6051  CTTCTTTCTT GTCTACGGAC AATATCGGTA CTCGTGAAAC ATTTCTTGCT 
    6101  GGTCTAATTG ATTCTGATGG CTATGTTACT GATGAGCATG GTATTAAAGC 
    6151  AACAATAAAG ACAATTCATA CTTCTGTCAG AGATGGTTTG GTTTCCCTTG 
    6201  CTCGTTCTTT AGGCTTAGTA GTCTCGGTTA ACGCAGAACC TGCTAAGGTT 
    6251  GACATGAATG TCACCAAACA TAAAATTAGT TATGCTATTT ATATGTCTGG 
    6301  TGGAGATGTT TTGCTTAACG TTCTTTCGAA GTGTGCCGGC TCTAAAAAAT 
    6351  TCAGGCCTGC TCCCGCCGCT GCTTTTGCAC GTGAGTGCCG CGGATTTTAT 
    6401  TTCGAGTTAC AAGAATTGAA GGAAGACGAT TATTATGGGA TTACTTTATC 
    6451  TGATGATTCT GATCATCAGT TTTTGCTTGG ATCCCAGGTT GTTGTACAGA 
    6501  ACAGAAGAGC TACTAGTTCG CGAGTCGACG GCGGCCGCGA ATTCCTCGAG 
    6551  CCCGGGTGAC TGCAGGAAGG GGATCCGGCT GCTAACAAAG CCCGAAAGGA 
    6601  AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG 
    6651  GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA 
    6701  TCCGGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG 
    6751  CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT 
    6801  AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC 
    6851  TACCGCATTA AAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG 
    6901  GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT 
    6951  CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC 
    7001  AGTTGCGCAG CCTGAATGGC GAATGGCAGC TTGGCTGTTT TGGCGGATGA 
    7051  GATAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT 
    7101  GATAAAACAG AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC 
    7151  CCATGCCGAA CTCAGAAGTG AAACGCCGTA GCGCCGATGG TAGTGTGGGG 
    7201  TCTCCCCATG CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG 
    7251  CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC 
    7301  GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA 
    7351  GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC 
    7401  ATCAAATTAA GC


[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Expression Plasmids]][[Category:NEB Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:lacI]][[Category:T7 Promoter]][[Category:MCS]]
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