View source for pGEM-3Z

==At a Glance==
[[File:PGEM-3Z.jpg|thumb|450px|pGEM-3Z, Image made with [[PlasMapper]]]]
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!align=left {{table heading style}} |Type
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Cloning
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!align=left {{table heading style}} |Origin of Replication
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!align=left {{table heading style}} |Copy #
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!align=left {{table heading style}} |Markers
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Ampicillin
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!align=left {{table heading style}} |Link to Sequence
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[http://www.ncbi.nlm.nih.gov/nuccore/7018282?report=fasta NCBI]
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!align=left {{table heading style}} |Features
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SP6 RNA polymerase promoter <br />
T7 RNA polymerase promoter<br />
MCS (multiple cloning site)
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!align=left {{table heading style}} |Accession Number
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7018282 X65304]

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==Notes==
The pGEM-3Z Vector is intended for use as a standard cloning vector, as well as for the synthesis of RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 allowing selection of recombinants by blue/white screening. 

In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/pgem-and-psp-subcloning-vectors/pgem_3z-vector/ Promega]

==References==
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{{RefHelp}}
<references />
<ref name='PMID:2985470'/>
==Sequence==
 1     GGGCGAATTC GAGCTCGGTA CCCGGGGATC CTCTAGAGTC GACCTGCAGG
 51    CATGCAAGCT TGAGTATTCT ATAGTGTCAC CTAAATAGCT TGGCGTAATC 
 101   ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
 151   ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
 201   GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
 251   GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA
 301   GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
 351   CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
 401   GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
 451   GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
 501   GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
 551   CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
 601   TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
 651   ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
 701   TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
 751   TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
 801   GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
 851   GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
 901   CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
 951   GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
 1001  TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
 1051  TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
 1101  CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
 1151  TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
 1201  TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
 1251  ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
 1301  GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
 1351  TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
 1401  CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
 1451  AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
 1501  TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
 1551  AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
 1601  CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
 1651  GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
 1701  CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
 1751  TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
 1801  TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
 1851  CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
 1901  ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
 1951  GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
 2001  ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
 2051  TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
 2101  CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
 2151  AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
 2201  TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
 2251  CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT
 2301  AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA
 2351  AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
 2401  CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC
 2451  GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG
 2501  AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT
 2551  ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG
 2601  CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG
 2651  TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC
 2701  GTTGTAAAAC GACGGCCAGT GAATTGTAAT ACGACTCACT ATA






[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:MCS]]
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