View source for pGEM-1

==At a Glance ==
[[Image:PGEM1.jpg|thumb|500px|right|pGEM-1, Key: magenta = promoters, red = Ampicillin marker]]
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!align=left {{table heading style}} |Type
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Cloning
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!align=left {{table heading style}} |Origin of Replication
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!align=left {{table heading style}} |Copy #
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!align=left {{table heading style}} |Markers
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Ampicillin
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!align=left {{table heading style}} |Link to Sequence
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[http://www.ncbi.nlm.nih.gov/nuccore/58196?report=fasta NCBI]
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!align=left {{table heading style}} |Features
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T7 Promoter<br />
SP6 Promoter<br />
multiple cloning sites<br />
lac operon<br />
Hind III, Pst  I, Sal  I, Acc  I, Sma  I, Hinc II, Xba  I, BamH I, Ava  I, Sac  I, EcoR I
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!align=left {{table heading style}} |Accession Number
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65300 X65300]

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==Notes==
The pGEM-1 vector contains SP6/T7 promoters flanking the pUC13 polylinker.  It is intended for use as a standard cloning vector as well as for the efficient synthesis of RNA in vitro. The plasmid carries the gene for ampicillin resistance.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources ==
[http://www.promega.com/products/pm/discontinued-vectors/#DV8 Promega]. Discontinued.

==References==
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<references />

==Sequence ==
<pre>
1     GAATACAAGC TTGGGCTGCA GGTCGACTCT AGAGGATCCC CGGGCGAGCT 
51    CGAATTCCGG TCTCCCTATA GTGAGTCGTA TTAATTTCGA TAAGCCAGCT 
101   GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
151   GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 
201   CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
251   ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
301   CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 
351   CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
401   CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
451   TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 
501   CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
551   CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
601   CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 
651   AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
701   GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
751   GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG 
801   CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
851   ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 
901   CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 
951   TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
1001  ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
1051  TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 
1101  TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 
1151  CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
1201  TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG 
1251  GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 
1301  AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
1351  GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 
1401  GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 
1451  ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
1501  TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 
1551  AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 
1601  TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
1651  CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 
1701  CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 
1751  GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
1801  CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 
1851  TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 
1901  GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
1951  TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
2001  CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 
2051  GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA 
2101  TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT 
2151  CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT 
2201  CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 
2251  CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC
2301  TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TCGACGCTCT 
2351  CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC 
2401  GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA 
2451  ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG
2501  CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC 
2551  GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA
2601  TGCGTCCGGC GTAGAGGATC TGGCTAGCGA TGACCCTGCT GATTGGTTCG 
2651  CTGACCATTT CCGGGGTGCG GAACGGCGTT ACCAGAAACT CAGAAGGTTC 
2701  GTCCAACCAA ACCGACTCTG ACGGCAGTTT ACGAGAGAGA TGATAGGGTC 
2751  TGCTTCAGTA AGCCAGATGC TACACAATTA GGCTTGTACA TATTGTCGTT 
2801  AGAACGCGGC TACAATTAAT ACATAACCTT ATGTATCATA CACATACGAT 
2851  TTAGGTGACA CTATA
</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]
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