View source for pET-5a

==At a Glance ==
[[Image:PET-5a.jpg|thumb|400px|right|pET-5a, Image provided by [http://www.promega.com/figures/popup.asp?fn=0957va Promega]]]
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4712e97d11c2e-->
<!--
******************************************************************************************
* 
*   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. ** 
* 
****************************************************************************************** -->
{| {{Prettytable}} width='30%' align=left  id="O4712e97d11c2e"  class=" tableEdit PlasmidTable"  

|-
!align=left {{table heading style}} |Type
||
Expression
|-
!align=left {{table heading style}} |Origin of Replication
||
pBR322
|-
!align=left {{table heading style}} |Copy #
||

|-
!align=left {{table heading style}} |Markers
||
Ampicillin
|-
!align=left {{table heading style}} |Link to Sequence
||
[http://www.promega.com/~/media/files/vector%20sequences/pet-5a.ashx?la=en Promega]
|-
!align=left {{table heading style}} |Features
||
BamHI cloning site<br />
Nde I cloning site<br />
Ribosome binding site<br />
T7 RNA polymerase promoter<br />
EcoRI cloning site 
|-
!align=left {{table heading style}} |Accession Number
||
None

|- class="tableEdit_footer" 
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.O4712e97d11c2e&page=113002&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4712e97d11c2e--></protect>
<BR>
<Br clear='all'>

==Notes==
Discontinued.

==History ==

==Sources ==
[http://www.promega.com/products/pm/discontinued-vectors/#DV7 Promega]

==References==
{{BacktoTop}}
{{RefHelp}}
<references />

==Sequence ==
    1  TTCGGATCCG CGACCCATTT GCTGTCCACC AGTCATGCTA GCCATATGTA
   51  TATCTCCTTC TTAAAGTTAA ACAAAATTAT TTCTAGAGGG AAACCGTTGT
  101  GGTCTCCCTA TAGTGAGTCG TATTAATTTC GCGGGATCGA GATCTCGATC
  151  CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA CAGGTGCGGT
  201  TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT CGGGCTCGCC
  251  ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT GGCAGGCCCC
  301  GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC
  351  GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC
  401  AGGAGTCGCA TAAGGGAGAG CGTCGACCGA TGCCCTTGAG AGCCTTCAAC
  451  CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT
  501  TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC
  551  TCTGGGTCAT TTTCGGCGAG GACCGCTTTC GCTGGAGCGC GACGATGATC
  601  GGCCTGTCGC TTGCGGTATT CGGAATCTTG CACGCCCTCG CTCAAGCCTT
  651  CGTCACTGGT CCCGCCACCA AACGTTTCGG CGAGAAGCAG GCCATTATCG
  701  CCGGCATGGC GGCCGACGCG CTGGGCTACG TCTTGCTGGC GTTCGCGACG
  751  CGAGGCTGGA TGGCCTTCCC CATTATGATT CTTCTCGCTT CCGGCGGCAT
  801  CGGGATGCCC GCGTTGCAGG CCATGCTGTC CAGGCAGGTA GATGACGACC
  851  ATCAGGGACA GCTTCAAGGA TCGCTCGCGG CTCTTACCAG CCTAACTTCG
  901  ATCATTGGAC CGCTGATCGT CACGGCGATT TATGCCGCCT CGGCGAGCAC
  951  ATGGAACGGG TTGGCATGGA TTGTAGGCGC CGCCCTATAC CTTGTCTGCC
 1001  TCCCCGCGTT GCGTCGCGGT GCATGGAGCC GGGCCACCTC GACCTGAATG
 1051  GAAGCCGGCG GCACCTCGCT AACGGATTCA CCACTCCAAG AATTGGAGCC
 1101  AATCAATTCT TGCGGAGAAC TGTGAATGCG CAAACCAACC CTTGGCAGAA
 1151  CATATCCATC GCGTCCGCCA TCTCCAGCAG CCGCACGCGG CGCATCTCGG
 1201  GCAGCGTTGG GTCCTGGCCA CGGGTGCGCA TGATCGTGCT CCTGTCGTTG
 1251  AGGACCCGGC TAGGCTGGCG GGGTTGCCTT ACTGGTTAGC AGAATGAATC
 1301  ACCGATACGC GAGCGAACGT GAAGCGACTG CTGCTGCAAA ACGTCTGCGA
 1351  CCTGAGCAAC AACATGAATG GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG
 1401  GAAACGCGGA AGTCAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC
 1451  AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC
 1501  GCTGGCATTG ACCCTGAGTG ATTTTTCTCT GGTCCCGCCG CATCCATACC
 1551  GCCAGTTGTT TACCCTCACA ACGTTCCAGT AACCGGGCAT GTTCATCATC
 1601  AGTAACCCGT ATCGTGAGCA TCCTCTCTCG TTTCATCGGT ATCATTACCC
 1651  CCATGAACAG AAATCCCCCT TACACGGAGG CATCAGTGAC CAAACAGGAA
 1701  AAAACCGCCC TTAACATGGC CCGCTTTATC AGAAGCCAGA CATTAACGCT
 1751  TCTGGAGAAA CTCAACGAGC TGGACGCGGA TGAACAGGCA GACATCTGTG
 1801  AATCGCTTCA CGACCACGCT GATGAGCTTT ACCGCAGCTG CCTCGCGCGT
 1851  TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT
 1901  CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG
 1951  CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT
 2001  AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT
 2051  GTACTGAGAG TGCACCATTG CGGTGTGAAA TACCGCACAG ATGCGTAAGG
 2101  AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
 2151  GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
 2201  TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA
 2251  GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
 2301  GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
 2351  CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
 2401  CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
 2451  CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
 2501  GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
 2551  GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
 2601  TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
 2651  CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
 2701  ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
 2751  ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
 2801  GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
 2851  GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
 2901  TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT
 2951  AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
 3001  TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
 3051  TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
 3101  TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
 3151  ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
 3201  GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG
 3251  CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
 3301  CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
 3351  TAGTTTGCGC AACGTTGTTG CCATTGCTGC AGGCATCGTG GTGTCACGCT
 3401  CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
 3451  GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
 3501  TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA
 3551  TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
 3601  TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
 3651  GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC
 3701  ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
 3751  AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
 3801  TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG
 3851  GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
 3901  ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
 3951  CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT
 4001  AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
 4051  CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG
 4101  GCGTATCACG AGGCCCTTTC GTCTTCAAGA A



[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Promega Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]]
__NOTOC__