View source for pET-14b

==At a Glance==
[[File:PET-14b.jpg|thumb|450px|pET-14b, Image made with [[PlasMapper]]]]

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!align=left {{table heading style}} |Type
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Cloning, Expression
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!align=left {{table heading style}} |Origin of Replication
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pBR322
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!align=left {{table heading style}} |Copy #
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~40
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!align=left {{table heading style}} |Markers
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Ampicillin
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!align=left {{table heading style}} |Link to Sequence
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[http://www.emdchemicals.com/life-science-research/pet-14b-dna/EMD_BIO-69660/p_uuid?attachments=VSEQ Novagen]
|-
!align=left {{table heading style}} |Features
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T7 Promoter<Br />
His tag<Br />
MCS<Br />
T7 Terminator<Br />
Thrombin cleavage site<Br />
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!align=left {{table heading style}} |Accession Number
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==Notes==

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.emdbiosciences.com/product/69660 Novagen]

==References {{HelpIcon|Help:Plasmid}}==
<references/>

==Sequence==
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 
      421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 
      481 cagcttcctt tcgggctttg ttagcagccg gatcctcgag catatggctg ccgcgcggca 
      541 ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta 
      601 aagttaaaca aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat 
      661 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc 
      721 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 
      781 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 
      841 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 
      901 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 
      961 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 
     1021 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 
     1081 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 
     1141 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc 
     1201 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg 
     1261 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt 
     1321 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat 
     1381 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc 
     1441 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg 
     1501 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca 
     1561 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca 
     1621 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt 
     1681 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca 
     1741 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag 
     1801 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa 
     1861 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc 
     1921 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct 
     1981 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct 
     2041 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac 
     2101 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc 
     2161 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat 
     2221 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat 
     2281 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat 
     2341 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg 
     2401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 
     2461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 
     2521 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 
     2581 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg 
     2641 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     2701 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     2761 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     2821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     2881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     2941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     3001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     3061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     3121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     3181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     3241 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     3301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     3361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     3421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     3481 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     3541 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     3601 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     3661 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     3721 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     3781 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     3841 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     3901 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     3961 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     4021 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     4081 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     4141 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     4201 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 
     4261 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     4321 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     4381 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     4441 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     4501 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     4561 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     4621 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a 




[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Ampicillin Marker Plasmids]]
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