View source for pCAL-kc

==At a Glance==
[[Image:Pcalkc.JPG|thumb|right|450px|pCAL-kc From [http://www.genomics.agilent.com/files/Vectors/Maps/pdf/pCAL-kc.pdf Agilent-Genomics]]]
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!align=left {{table heading style}} |Type
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Expression
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!align=left {{table heading style}} |Origin of Replication
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pBR322 
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!align=left {{table heading style}} |Copy #
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|-
!align=left {{table heading style}} |Markers
||
Ampicillin
|-
!align=left {{table heading style}} |Link to Sequence
||
[http://www.genomics.agilent.com/files/Vectors/Sequences/pcal-kc_s.txt Agilent-Genomics]
|-
!align=left {{table heading style}} |Features
||
T7 Promoter<Br />

lac Operator<Br />

MCS<Br />

lacI repressor ORF<Br />
|-
!align=left {{table heading style}} |Accession Number
||


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==Notes==
Express affinity tagged fusion protein in bacteria for enrichment of Calmodulin based resin.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.stratagene.com/lit/vector.aspx Agilent-Genomics]

==References==
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<references />

==Sequence==

    1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
   51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG GCTAGCATGA  
  101  CTGGTGGACA GCAAATGGGT CGGATCCCTT AGACGCGCAT CACTTGGTAG  
  151  ATCCATGTAT CCACGTGGGA ATGGTACCAA GCGACGATGG AAAAAGAATT  
  201  TCATAGCCGT CTCAGCAGCC AACCGCTTTA AGAAAATCTC ATCCTCCGGG  
  251  GCACTTTGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG  
  301  CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG  
  351  GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCCGC  
  401  AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG  
  451  GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG  
  501  GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC  
  551  TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC  
  601  TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT  
  651  TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG  
  701  TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC  
  751  CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA  
  801  CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT  
  851  TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT  
  901  TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC  
  951  CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA  
 1001  AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC  
 1051  AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA  
 1101  CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG  
 1151  CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA  
 1201  CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG  
 1251  GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT  
 1301  ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT  
 1351  TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA  
 1401  TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC  
 1451  GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC  
 1501  GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC  
 1551  CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG  
 1601  AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC  
 1651  TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT  
 1701  TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC  
 1751  CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG  
 1801  AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC  
 1851  TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA  
 1901  TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC  
 1951  AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC  
 2001  AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC  
 2051  AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA  
 2101  GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG  
 2151  TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT  
 2201  ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG  
 2251  ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG  
 2301  CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA  
 2351  CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC  
 2401  TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC  
 2451  TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA  
 2501  TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA  
 2551  CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG  
 2601  CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG  
 2651  TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC  
 2701  ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC  
 2751  AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT  
 2801  ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA  
 2851  CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG  
 2901  GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT  
 2951  TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG  
 3001  TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG  
 3051  ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA  
 3101  CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG  
 3151  GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA  
 3201  GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA  
 3251  GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT  
 3301  GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA  
 3351  TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC  
 3401  GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG  
 3451  CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA  
 3501  GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG  
 3551  ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC  
 3601  CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG  
 3651  GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG  
 3701  ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG  
 3751  CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC  
 3801  AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG  
 3851  AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA  
 3901  ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG  
 3951  GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC  
 4001  GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG  
 4051  CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC  
 4101  GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC  
 4151  GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA  
 4201  AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG  
 4251  AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GAGATCCCGG  
 4301  TGCCTAATGA GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG  
 4351  CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA  
 4401  CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCCAGGGTG GTTTTTCTTT  
 4451  TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA  
 4501  GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC  
 4551  CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT  
 4601  CGTCGTATCC CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG  
 4651  GTAATGGCGC GCATTGCGCC CAGCGCCATC TGATCGTTGG CAACCAGCAT  
 4701  CGCAGTGGGA ACGATGCCCT CATTCAGCAT TTGCATGGTT TGTTGAAAAC  
 4751  CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG CTGAATTTGA  
 4801  TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC  
 4851  AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA  
 4901  CCAGATGCTC CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA  
 4951  CTGTTGATGG GTGTCTGGTC AGAGACATCA AGAAATAACG CCGGAACATT  
 5001  AGTGCAGGCA GCTTCCACAG CAATGGCATC CTGGTCATCC AGCGGATAGT  
 5051  TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG CACCGCCGCT  
 5101  TTACAGGCTT CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC  
 5151  ACCCAGTTGA TCGGCGCGAG ATTTAATCGC CGCGACAATT TGCGACGGCG  
 5201  CGTGCAGGGC CAGACTGGAG GTGGCAACGC CAATCAGCAA CGACTGTTTG  
 5251  CCCGCCAGTT GTTGTGCCAC GCGGTTGGGA ATGTAATTCA GCTCCGCCAT  
 5301  CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC AGAAACGTGG CTGGCCTGGT  
 5351  TCACCACGCG GGAAACGGTC TGATAAGAGA CACCGGCATA CTCTGCGACA  
 5401  TCGTATAACG TTACTGGTTT CACATTCACC ACCCTGAATT GACTCTCTTC  
 5451  CGGGCGCTAT CATGCCATAC CGCGAAAGGT TTTGCGCCAT TCGATGGTGT  
 5501  CCGGGATCTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC  
 5551  CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT  
 5601  GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA  
 5651  CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC  
 5701  CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC  
 5751  CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC  
 5801  CCGCGAAAT


[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Stratagene Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]][[Category:lac Operator]][[Category:MCS]][[Category:Agilent Plasmids]]
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