View source for pCAL-c

==At a Glance==
[[Image:Pcalc.JPG|thumb|450px|right|pCAL-c Image provided by [http://www.genomics.agilent.com/files/Vectors/Maps/pdf/pCAL-c.pdf Agilent-Genomics]]]
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!align=left {{table heading style}} |Type
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Expression
|-
!align=left {{table heading style}} |Origin of Replication
||
pBR322
|-
!align=left {{table heading style}} |Copy #
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|-
!align=left {{table heading style}} |Markers
||
Ampicillin
|-
!align=left {{table heading style}} |Link to Sequence
||
[http://www.genomics.agilent.com/files/Vectors/Sequences/pcal-c_s.txt Agilent-Genomics]
|-
!align=left {{table heading style}} |Features
||
T7 Promoter<Br />
lac Operator<Br />
MCS<Br />
lacI repressor ORF<Br />
|-
!align=left {{table heading style}} |Accession Number
||


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==Notes==
Express affinity tagged fusion protein in bacteria for enrichment of Calmodulin based resin.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.stratagene.com/lit/vector.aspx Agilent-Genomics]

==References==
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{{RefHelp}}
<references />

==Sequence==


    1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
   51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG GCTAGCATGA  
  101  CTGGTGGACA GCAAATGGGT CGGATCCATG TATCCACGTG GGAATGGTAC  
  151  CAAGCGACGA TGGAAAAAGA ATTTCATAGC CGTCTCAGCA GCCAACCGCT  
  201  TTAAGAAAAT CTCATCCTCC GGGGCACTTT GATCCGGCTG CTAACAAAGC  
  251  CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT  
  301  AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA  
  351  GGAACTATAT CCGGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC  
  401  AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG  
  451  CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT  
  501  GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA  
  551  GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA  
  601  ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA  
  651  AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT  
  701  GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA  
  751  AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT  
  801  TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA  
  851  AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC  
  901  TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT  
  951  TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC  
 1001  CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC  
 1051  AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT  
 1101  GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA  
 1151  CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA  
 1201  CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG  
 1251  GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT  
 1301  GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC  
 1351  TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA  
 1401  GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC  
 1451  TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC  
 1501  TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG  
 1551  AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC  
 1601  CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC  
 1651  TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG  
 1701  ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT  
 1751  CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC  
 1801  CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA  
 1851  CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA  
 1901  GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT  
 1951  AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC  
 2001  CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC  
 2051  GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC  
 2101  GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG  
 2151  ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC  
 2201  GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG  
 2251  GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT  
 2301  TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG  
 2351  ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT  
 2401  TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT  
 2451  GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC  
 2501  TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG  
 2551  AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC  
 2601  GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT  
 2651  GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC  
 2701  ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC  
 2751  TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA  
 2801  GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG  
 2851  CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC  
 2901  CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT  
 2951  GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC  
 3001  ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC  
 3051  GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG  
 3101  CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG  
 3151  CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC  
 3201  AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC  
 3251  GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA  
 3301  ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT  
 3351  GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT  
 3401  AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC  
 3451  ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG  
 3501  CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT  
 3551  TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT  
 3601  GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG  
 3651  GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA  
 3701  TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG  
 3751  CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG  
 3801  GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC  
 3851  CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG  
 3901  CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC  
 3951  AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG  
 4001  CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA  
 4051  GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG  
 4101  CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC  
 4151  CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA  
 4201  TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC  
 4251  AAGGGCATCG GTCGAGATCC CGGTGCCTAA TGAGTGAGCT AACTTACATT  
 4301  AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC  
 4351  AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT  
 4401  GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT  
 4451  TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG  
 4501  GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT  
 4551  ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC GAGATATCCG  
 4601  CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC  
 4651  ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG  
 4701  CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC  
 4751  GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA  
 4801  GCCAGACGCA GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC  
 4851  GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC AGTCGCGTAC  
 4901  CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA  
 4951  TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC  
 5001  ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG  
 5051  CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT  
 5101  ACCATCGACA CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT  
 5151  CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG GAGGTGGCAA  
 5201  CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC CACGCGGTTG  
 5251  GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT  
 5301  CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG  
 5351  AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC  
 5401  ACCACCCTGA ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA  
 5451  GGTTTTGCGC CATTCGATGG TGTCCGGGAT CTCGACGCTC TCCCTTATGC  
 5501  GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC  
 5551  CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC  
 5601  CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC  
 5651  CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC  
 5701  GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG  
 5751  CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT



[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Stratagene Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]][[Category:lac Operator]][[Category:MCS]][[Category:Agilent Plasmids]]
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