View source for PMID:2165476

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!align=left  |Citation
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'''Tormo, A, Almirón, M and Kolter, R'''  (1990) surA, an Escherichia coli gene essential for survival in stationary phase. ''J. Bacteriol.'' '''172''':4339-47
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!align=left  |Abstract
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Mutations in genes not required for exponential growth but essential for survival in stationary phase were isolated in an effort to understand the ability of wild-type Escherichia coli cells to remain viable during prolonged periods of nutritional deprivation. The phenotype of these mutations is referred to as Sur- (survival) and the genes are designated sur. The detailed analysis of one of these mutations is presented here. The mutation (surA1) caused by insertion of a mini-Tn10 element defined a new gene located near 1 min on the E. coli chromosome. It was located directly upstream of pdxA and formed part of a complex operon. Evidence is presented supporting the interpretation that cells harboring the surA1 mutation die during stationary phase while similar insertion mutations in other genes of the operon do not lead to a Sur- phenotype. Strains harboring surA1 had a normal doubling time in both rich and minimal medium, but cultures lost viability after several days in stationary phase. Analysis of revertants and suppressors of surA1, which arose after prolonged incubation in stationary phase, indicates that DNA rearrangements (excisions and duplications) occurred in cultures of this strain even when the viable-cell counts were below 10(2) cells per ml. Cells containing suppressing mutations then grew in the same culture to 10(8) cells per ml, taking over the population. The implications of these observations to our understanding of stationary-phase mutagenesis are discussed.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2165476 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC213259 PMC213259]

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!align=left  |Keywords
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DNA Transposable Elements; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial; Kanamycin/pharmacology; Kinetics; Mutation; Phenotype; Restriction Mapping; Suppression, Genetic

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