View source for Hi-Control(tm) 10G

{{See|Help:Strains|strains}}

==Alternate Names==

==Sources==

==Chromosomal Genotype==
<em>mcrA</em> &Delta;(<em>mrr-hsd</em>RMS-<em>mcr</em>BC) <em>end</em>A1 <em>rec</em>A1 &Phi;80<em>dlac</em>Z&Delta;M15 &Delta;<em>lac</em>X74 <em>ara</em>D139 &Delta;(<em>ara,leu</em>)7697 <em>gal</em>U <em>gal</em>K <em>rps</em>L <em>nup</em>G &lambda;- <em>ton</em>A

==Plasmids==
Mini-F <em>lacI<sup>q1</sup></em>(Gent<sup>r</sup>)
==Phage==

==Phenotypes==

==Construction==

==Notes==
*The HI-Control 10G cells contain a single-copy BAC plasmid harboring a specially engineered version of the <em>lacI<sup>q1</sup></em> repressor allele. The <em>lacI<sup>q1</sup></em> allele expresses ~170-fold more lac repressor protein than the wild-type <em>lacI</em> gene.
*For stable cloning of T7 protein expression plasmids.
*Resistant to phage T1.


==References==
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[[Category:Strains]]
[[Category:Cloning Strains]]
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