View source for BW25113

#REDIRECT [[Category:Strain:BW25113]]
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==Alternate Names ==

==Chromosomal Genotype ==
∆''(araD-araB)567'', ∆''lacZ4787''(::rrnB-3), lambda<sup>-</sup>, ''rph-1'', ∆''(rhaD-rhaB)568'', ''hsdR514''
==Plasmids ==

==Phage ==

==Phenotypes ==

==Construction ==
BW25113 is a derivative of the F<sup>-</sup>, λ<sup>-</sup>, ''E. coli'' K-12 strain [http://cgsc2.biology.yale.edu/Strain.php?ID=7024 BD792 (CGSC6159)]<ref name='PMID:10829079'/>, which is a derivative of ''E. coli'' K-12 W1485.  According to the CGSC, BD792 carries ''rpoS396''(Am), but this mutation is not listed in the published genotype for BW25113.

==Notes==
BW25113 was used to make the Keio Collection of single-gene knockouts. <ref name=PMID:16738554/>
<ref name=PMID:10829079/>  Available from the Coli Genetics Stock Center: [http://cgsc2.biology.yale.edu/Strain.php?ID=64667 CGSC#7636]
The genotype for BW25113 in Datsenko et al. <ref name=PMID:10829079/> said the strain is lacI<sup>Q</sup>, but it was later shown to be lacI<sup>+</sup> Baba et al. (Supplementary Table 1)<ref name=PMID:16738554/>.

According to the CGSC, ''lacZ4787(del)'' has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of ''lacZ'' through the promoter.

==References==
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<references/>

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[[Category:Strains]] [[Category:Strain:W1485]]