View source for pLysS

==At a Glance==
[[Image:#pLysE & pLysS.JPG|thumb|right|300px|pLysE & pLysS, image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB107.pdf Novagen]]]
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.E47025937a0982-->
<!--
******************************************************************************************
*
*   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*
****************************************************************************************** -->
{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Expression
|-
!{{table heading style}} |Origin of Replication
|
p15A
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Chloramphenicol
|-
!{{table heading style}} |Link to Sequence
|
[http://www.emdbiosciences.com/docs/NDIS/69659-000.HTML Novagen]
|-
!{{table heading style}} |Features
|
T7 lysozyme coding seq. (pLysS)<br>
T7 lysozyme coding seq. (pLysE)
|-
!{{table heading style}} |Accession Number
|

|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.E47025937a0982&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.E47025937a0982--></protect>
<BR>
<Br clear='all'>
==Notes==
This plasmid is not a cloning vector; it is used in lDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.emdbiosciences.com/product/69659 Novagen]

==References==
<references/>

==Sequence==
       1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG 
      51 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 
     101 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC 
     151 CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC 
     201 CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT 
     251 AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT 
     301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC 
     351 AGGGCTTCCC GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG 
     401 TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT CGGGTGATGC 
     451 TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG 
     501 GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT 
     551 GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
     601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG 
     651 CAGGAGAAAA AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA 
     701 TATATTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT CGTTCGACTG 
     751 CGGCGAGCGG AAATGGCTTA CGAACGGGGC GGAGATTTCC TGGAAGATGC 
     801 CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA AGCCGTTTTT 
     851 CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC 
     901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCTG 
     951 GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC
    1001 ATTCCGCTGT TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT 
    1051 CCGGGTAGGC AGTTCGCTCC AAGCTGGACT GTATGCACGA ACCCCCCGTT 
    1101 CAGTCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
    1151 GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT AATTGATTTA 
    1201 GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC 
    1251 AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT
    1301 TGGTAGCTCA GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG 
    1351 TTTTCAGAGC AAGAGATTAC GCGCAGACCA AAACGATCTC AAGAAGATCA 
    1401 TCTTATTAAT CAGATAAAAT ATTTCTAGAT TTCAGTGCAA TTTATCTCTT 
    1451 CAAATGTAGC ACCTGAAGTC AGCCCCATAC GATATAAGTT GTAATTCTCA 
    1501 TGTTTGACAG CTTATCATCG ATAAGCTTTA ATGCGGTAGT TTATCACAGT 
    1551 TAAATTGCTA ACGCAGTCAG GCACCGTGTA TGAAATCTAA CAATGCGCTC 
    1601 ATCGTCATCC TCGGCACCGT CACCCTGGAT GCTGTAGGCA TAGGCTTGGT 
    1651 TATGCCGGTA CTGCCGGGCC TCTTGCGGGA TATCGTCCAT TCCGACAGCA 
    1701 TCGCCAGTCA CTATGGCGTG CTGCTAGCGC TATATGCGTT GATGCAATTT 
    1751 CTATGCGCAC CCGTTCTCGG AGCACTGTCC GACCGCTTTG GCCGCCGCCC 
    1801 AGTCCTGCTC GCTTCGCTAC TTGGAGCCAC TATCGACTAC GCGATCATGG 
    1851 CGACCACACC CGTCCTGTGG ATCCGGCCCA TTGGCTGCCT CCCACACTTG 
    1901 GATATGCCTC CTCGGAGCCT TATAGAATTG TTTATAAGAC TTGCGCATTA 
    1951 TTTGACCTCC AATGCGAACA AAGGGAAACC GCTGTGGTCT CCCTTTAGTG 
    2001 AGTTCAATTA ATTATCCACG GTCAGAAGTG ACCAGTTCGT TCTTCTCCCA 
    2051 CCAACGCTTA AGGTCGAACG AAGGGCAAGC CTTCGGCGCC ACCTCATGAT 
    2101 GGGCGCGAAG ACCAGCGCCT TCGTACTTAG CCAGCAGTGT GACAAGCAGT 
    2151 GAGCGAAGGG ATTGCATTTG GGCTGGCGTA AAGTTAGCGT CGAACTTACC 
    2201 TTTATCGTCG ATACCACCAA CAAGGCAGAC GCCGATAGAG TTGTGGTTGT 
    2251 AACCCTTAGC GTGAGAGCCT ACAGCCATCT CATCTCGTCC TGCCTCCACA 
    2301 GTACCGTCTC GCTTGATGAT AAAGTGGTAT CCCACATCGA GCCAACCCTG 
    2351 CTCTTTGTGC CACTGGCGAA TCTCACGGAC ACCAACATTC TGACTTGGCT 
    2401 TGGTAGCCGA GCAGTGAACA AAGATTGCGT CAGTAGATTC ACGTTGTTTA 
    2451 AACTGTACAC GAGCCATTAT TTCTTTCCTC CTTTCCTTTT TAATCTATCA 
    2501 AAGGGGACCC GGATCCTCTA CGCCGGACGC ATCGTGGCCG GCATCACCGG 
    2551 CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC ACCGATGGGG 
    2601 AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT 
    2651 ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA 
    2701 TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG
    2751 GCTGCTTCCT AATGCAGGAG TCGCATAAGG GAGAGCGTCG ACCGATGCCC 
    2801 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC 
    2851 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC 
    2901 AGGTGCCGGC AGCGCTCTGG GTCATTTTCG GCGAGGACCG CTTTCGCTGG 
    2951 AGCGCGACGA TGATCGGCCT GTCGCTTGCG GTATTCGGAA TCTTGCACGC 
    3001 CCTCGCTCAA GCCTTCGTCA CTGGTCCCGC CACCAAACGT TTCGGCGAGA 
    3051 AGCAGGCCAT TATCGCCGGC ATGGCGGCCG ACGCGCTGGG CTACGTCTTG 
    3101 CTGGCGTTCG CGACGCGAGG CTGGATGGCC TTCCCCATTA TGATTCTTCT 
    3151 CGCTTCCGGC GGCATCGGGA TGCCCGCGTT GCAGGCCATG CTGTCCAGGC 
    3201 AGGTAGATGA CGACCATCAG GGACAGCTTC AAGGATCGCT CGCGGCTCTT 
    3251 ACCAGCCTAA CTTCGATCAC TGGACCGCTG ATCGTCACGG CGATTTATGC 
    3301 CGCCTCGGCG AGCACATGGA ACGGGTTGGC ATGGATTGTA GGCGCCGCCC 
    3351 TATACCTTGT CTGCCTCCCC GCGTTGCGTC GCGGTGCATG GAGCCGGGCC 
    3401 ACCTCGACCT GAATGGAAGC CGGCGGCACC TCGCTAACGG ATTCACCACT 
    3451 CCAAGAATTG GAGCCAATCA ATTCTTGCGG AGAACTGTGA ATGCGCAAAC 
    3501 CAACCCTTGG CAGAACATAT CCATCGCGTC CGCCATCTCC AGCAGCCGCA 
    3551 CGCGGCGCAT CTCGGGCAGC GTTGGGTCCT GGCCACGGGT GCGCATGATC 
    3601 GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG 
    3651 TTAGCAGAAT GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT 
    3701 GCAAAACGTC TGCGACCTGA GCAACAACAT GAATGGTCTT CGGTTTCCGT 
    3751 GTTTCGTAAA GTCTGGAAAC GCGGAAGTCC CCTACGTGCT GCTGAAGTTG 
    3801 CCCGCAACAG AGAGTGGAAC CAACCGGTGA TACCACGATA CTATGACTGA 
    3851 GAGTCAACGC CATGAGCGGC CTCATTTCTT ATTCTGAGTT ACAACAGTCC 
    3901 GCACCGCTGT CCGGTAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT 
    3951 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC 
    4001 CGGCAGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG 
    4051 CCGAAACAAG CGCCCTGCAC CATTATGTTC CGGATCTGCA TCGCAGGATG 
    4101 CTGCTGGCTA CCCTGTGGAA CACCTACATC TGTATTAACG AAGCGCTAAC 
    4151 CGTTTTTATC AGGCTCTGGG AGGCAGAATA AATGATCATA TCGTCAATTA 
    4201 TTACCTCCAC GGGGAGAGCC TGAGCAAACT GGCCTCAGGC ATTTGAGAAG 
    4251 CACACGGTCA CACTGCTTCC GGTAGTCAAT AAACCGGTAA ACCAGCAATA 
    4301 GACATAAGCG GCTATTTAAC GACCCTGCCC TGAACCGACG ACCGGGTCGA 
    4351 ATTTGCTTTC GAATTTCTGC CATTCATCCG CTTATTATCA CTTATTCAGG 
    4401 CGTAGCACCA GGCGTTTAAG GGCACCAATA ACTGCCTTAA AAAAATTACG 
    4451 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 
    4501 CCGACATGGA AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG 
    4551 CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG 
    4601 GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA 
    4651 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT 
    4701 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA 
    4751 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 
    4801 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC 
    4851 CCATATCACC AGCTCACCGT CTTTCATTGC CATACG 




[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Chloramphenicol Marker Plasmids]][[Category:T7 lysozyme]]
__NOTOC__