View source for pSP73

==At a Glance ==
[[Image:#PSP73.jpg|thumb|400px|right|pSP73, Image provided by [http://www.promega.com/figures/popup.asp?fn=0300va&partno=P2221&product=pSP73+Vector Promega]]]
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!{{table heading style}} |Type
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Cloning
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!{{table heading style}} |Origin of Replication
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!{{table heading style}} |Copy #
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!{{table heading style}} |Markers
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Ampicillin
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!{{table heading style}} |Link to Sequence
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[http://www.promega.com/vectors/psp73.txt Promega]
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!{{table heading style}} |Features
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SP6 promoter<br>
T7 promoter<br>           
multiple cloning region<br>                   
Bgl  II, EcoR V, Cla  I, EcoR I, Sac  I, Kpn  I, Sma  I, Xba  I, BamH I, Sal  I, Acc  I, Pst  I, Sph  I, Hind III, Pvu  II,  Xho 
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!{{table heading style}} |Accession Number
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6759495 X65333]
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==Notes==
The pSP73 vector can be used as a standard cloning vector and transcription of RNA in vitro. It contains the SP6 and T7 RNA polymerase promoters which flank a unique multiple cloning region. This region includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The two vectors pSP72 and pSP73 are identical except for the orientation of the mutiple cloning site region.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources ==
[http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_222]

==References==
<ref name='PMID:2828872'/>
<references/>

==Sequence ==
<pre>
1     GAACCAGATC TGATATCATC GATGAATTCG AGCTCGGTAC CCGGGGATCC
51    TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT CAGCTGCTCG AGGCCGGTCT
101   CCCTATAGTG AGTCGTATTA ATTTCGATAA GCCAGGTTAA CCTGCATTAA
151   TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
201   CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
251   CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
301   GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
351   CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
401   ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
451   GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
501   TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
551   CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
601   GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
651   GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
701   TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
751   AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
801   CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
851   CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
901   ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
951   AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
1001  CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
1051  AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
1101  AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
1151  TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
1201  GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
1251  TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
1301  ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
1351  CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
1401  TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
1451  CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
1501  TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
1551  AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
1601  CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
1651  GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
1701  GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
1751  CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
1801  ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
1851  GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
1901  CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
1951  GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
2001  CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
2051  GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
2101  CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
2151  CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG
2201  CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
2251  ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
2301  GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
2351  CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGGACAT ATTGTCGTTA
2401  GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACGATT
2451  TAGGTGACAC TATA  

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]
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