View source for SG4121

{| class="wikitable" border="1"
|-
| Name
| SG4121
|-
| Synonyms
| 
|-
| Genotype
| lon-100 Δ(gal-pgl)324 lac thi rpsL recA hsdR
|-
| Phenotype
| Streptomycin resistant, non-mucoid, Lon<sup>-</sup>
|-
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%20671 LMBP 671] 
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=1312934 PMID:1312934]
|}

===Notes===
The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology.<br />
Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli (see e.g. Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688).<br /> 
In addition, this strain is K12 restriction negative / modification positive, and RecA deficient.



[[Category:BCCM/GeneCorner]][[Category:Strains]]