View source for M5219

{| class="wikitable" border="1"
|-
| Name
| KM5219
|-
| Synonyms
| 
|-
| Genotype
| lacZam trpAam rpsL lambda(bio252 cI857 ΔH1)
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%20130 LMBP 130]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/6271633 PMID:6271633]
|}

===Notes===
Expression host for plasmids containing phage lambda's PL or PR promoter. Induction is obtained by shifting the culture from 28°C to 42°C. The resident lambda is a defective lysogen. ΔH1 removes part of cro and all genes to the right of it. bio252 removes all genes to the left of cIII. cI857 is ind<sup>-</sup>. At 42°C, N is expressed from the lysogen. As a consequence, transcription termination signals that might be present downstream from the PL promoter and the nutL site, as present on the pPL-series of plasmids, can be overridden.
Useful for the expression of cloned genes carrying transcription terminators between the start of the gene and the insertion point.

 
[[Category:BCCM/GeneCorner]][[Category:Strains]]