View source for MC1061λ

{| class="wikitable" border="1"
|-
| Name
| MC1061λ
|-
| Synonyms
| MC1061lambda
|-
| Genotype
| ∆(araA-leu)7697 [araD139]<sub>B/r</sub> ∆(codB-lacI)3 = ∆lac74 galK16 galE15 e14- mcrA0 relA1 rpsL150 spoT1 mcrB1 hsdR2 λ+
|-
| Phenotype
| Streptomycin resistant, zeocin resistant
|-
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%201061 LMBP 1061] 
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=7590329 PMID:7590329] [http://www.ncbi.nlm.nih.gov/pubmed/?term=6997493 PMID:6997493] 
|}

===Notes===
Useful host for primary transformation.  Transforms very well by the CaCl<sub>2</sub> method (10<sup>7</sup>/µg).  There is no Type I restriction; incoming DNA receives the E. coli K modification.<br />
Lysogenized with λ wild type.<br />
Recommended as maintenance host for plasmids containing the PL or PR promoter from phage λ.<br />
It is recommended to grow MC1061(λ), containing pPL plasmids, at 28°C.<br />
As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as ''tac'', ''trc'', ''N25/O2'' … promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability.<br />
The zeocin resistance is most probably related to the presence of a functional recA gene (recA<sup>+</sup>).


[[Category:BCCM/GeneCorner]][[Category:Strains]]