pET-28b(+)
At a Glance
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pET-28a-c(+), image provided by Novagen
<protect>
| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
f1 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
The pET-28b(+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. The T7 expression region is reversed on the circular map due to the sequence numbering from the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, the T7 terminator primer should be used when performing single stranded sequencing.
History
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Sources
References
- ↑ Suárez, T et al. (2002) Biochemical defects in retina-specific human ATP binding cassette transporter nucleotide binding domain 1 mutants associated with macular degeneration. J. Biol. Chem. 277 21759-67 PubMed
- ↑ Kadkhodayan, S et al. (2000) Cloning, expression, and one-step purification of the minimal essential domain of the light chain of botulinum neurotoxin type A. Protein Expr. Purif. 19 125-30 PubMed
- ↑ Lecker, SH et al. (1999) Ubiquitin conjugation by the N-end rule pathway and mRNAs for its components increase in muscles of diabetic rats. J. Clin. Invest. 104 1411-20 PubMed
- ↑ Wang, D & Parrish, CR (1999) A heterogeneous nuclear ribonucleoprotein A/B-related protein binds to single-stranded DNA near the 5' end or within the genome of feline parvovirus and can modify virus replication. J. Virol. 73 7761-8 PubMed
- ↑ Chaussivert, N et al. (1995) Complex interactions between yeast TFIIIB and TFIIIC. J. Biol. Chem. 270 15353-8 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat
241 ggctgccgcg cggcaccagg ccgctgctgt gatgatgatg atgatggctg ctgcccatgg
301 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
361 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
421 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
481 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
541 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
601 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
661 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt
721 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
781 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
841 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
901 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
961 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
1021 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
1081 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
1141 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
1201 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
1261 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
1321 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
1381 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
1441 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
1501 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
1561 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
1621 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
1681 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
1741 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
1801 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
1861 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag
1921 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
1981 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
2041 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
2101 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
2161 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct
2221 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
2281 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
2341 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
2401 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
2461 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
2521 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
2581 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
2641 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
2701 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
2761 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
2821 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
2881 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
2941 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
3001 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa
3061 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
3121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
3181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
3241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
3301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
3361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
3421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
3481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
3541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
3601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
3661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
3721 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
3781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
3841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
3901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg
3961 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
4021 tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
4081 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
4141 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
4201 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
4261 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
4321 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
4381 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
4441 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
4501 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
4561 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
4621 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
4681 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
4741 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
4801 tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca
4861 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat
4921 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
4981 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
5041 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
5101 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
5161 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
5221 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
5281 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
5341 gccgctacag ggcgcgtccc attcgcca
