pET-23(+)

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At a Glance

pET-23(+), Image made with PlasMapper

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Type

Expression

Origin of Replication

pBR322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter
Multiple cloning sites (BamH I - Xho I)
His•Tag

Accession Number


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Notes

pET-23(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It therefore lacks the ribosome binding site and ATG start codon present on the pET translation vectors.

Sources

Novagen

References

See Help:References for how to manage references in EcoliWiki.


Sequence

        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
      181 cgacggagct cgaattcgga tcctagaggg aaaccgttgt ggtctcccta tagtgagtcg 
      241 tattaatttc gcgggatcga gatctcgggc agcgttgggt cctggccacg ggtgcgcatg 
      301 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag 
      361 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc 
      421 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag 
      481 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt 
      541 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg 
      601 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt 
      661 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc 
      721 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt 
      781 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg 
      841 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac 
      901 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 
      961 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 
     1021 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga 
     1081 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat 
     1141 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 
     1201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     1261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     1321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     1381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     1441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     1501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     1561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     1621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     1681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     1741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     1801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     1861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     1921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     1981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     2041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     2101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     2161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     2221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     2281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     2341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     2401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt 
     2461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     2521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     2581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     2641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     2701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     2761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     2821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     2881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     2941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     3001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     3061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     3121 acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
     3181 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
     3241 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
     3301 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
     3361 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
     3421 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
     3481 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
     3541 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca

Categoty:Plasmids