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...gged E. coli DNA photolyase by RP-HPLC and SE-HPLC. ''J. Biochem. Biophys. Methods'' '''63''':111-24 ...rformance liquid chromatography and size-exclusion high-performance liquid chromatography were developed to measure the enzyme activity. The enzyme was found to be a ...

...Luster, W, Cvetanović, M and Kunau, WH''' (1982) Purification by affinity chromatography of 2,4-dienoyl-CoA reductases from bovine liver and Escherichia coli. ''Eur ...adienoyl-CoA. 5. The application of dye-ligand and general ligand affinity chromatography for purification of NADPH-dependent 2,4-dienoyl-CoA reductases taking part ...

...tion; Chromatography; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Ion Exchange; Cyclohexanecarboxylic Acids; Cyclohexanes; Electrophoresis, == Useful Materials and Methods {{HelpIcon|Help:Literature}}== ...

...ion of elongation factors EF-Tu and EF-G from Escherichia coli by covalent chromatography on thiol-sepharose. ''Protein Expr. Purif.'' '''14''':65-70 ...ng both factors were loaded on the thiol-Sepharose column. Thiol-Sepharose chromatography efficiently separates each elongation factor from all contaminating protein ...

...Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography. ''Electrophoresis'' '''20''':2181-95 ...ons. The results include a list of the proteins enriched by hydroxyapatite chromatography and a two-dimensional map of the enriched proteins. They may be useful in t ...

...the Escherichia coli proteome by chromatography on reactive dye columns. ''Methods Mol. Biol.'' '''424''':167-85 ...on using standard peptide mass mapping and MALDI-TOF MS methods. Under the chromatography conditions employed up to 4.42% of the proteins present in the total nonfra ...

...on-exchange chromatography using DEAE-Servacell, and two FPLC ion-exchange chromatography runs: the first without urea, the second in its presence. This method allow ...genetics; Spectrin/metabolism; Spectrometry, Mass, Electrospray Ionization/methods ...

...CM''' (2007) Lipid composition of membranes of Escherichia coli by liquid chromatography/tandem mass spectrometry using negative electrospray ionization. ''Rapid Co ...zation tandem mass spectrometry (LC/ESI-MS/MS) method using reversed-phase chromatography was developed for the analysis of phospholipids from bacterial extracts of ...

...cin enantiomers in sewage using two-step solid-phase extraction and liquid chromatography with fluorescence detection. ''J Chromatogr A'' '''1182''':77-84 ...ples based on LC-FL method were consistent with those obtained upon liquid chromatography using tandem mass spectrometry (LC-MS/MS) method. ...

...ly purified Escherichia coli molecular chaperone Cpn60 (GroEL) by affinity chromatography and urea-induced monomerization. ''Biochim. Biophys. Acta'' '''1252''':69-7 ...yellow columns. Pure substrate-free GroEL was obtained after ion-exchange chromatography of GroEL monomers followed by reassembly of the purified monomers into func ...

...PBP 1A from PBP 1Bs is achieved in a second step employing cation exchange chromatography yielding enzymatically active native murein polymerases. == Useful Materials and Methods {{HelpIcon|Help:Literature}}== ...

...li B-specific p-aminobenzoate "pick-up" protein to homogeneity by affinity chromatography. ''J. Chromatogr.'' '''115''':205-12 ...y sodium dodecyl sulphate gel electrophoresis and Sephadex G-75 gel column chromatography. p-Aminobenzoate and sulphonamide ligand binding studies showed a single bi ...

...e aerobic respiratory chain of Escherichia coli by high-performance liquid chromatography and its application to analysis of mitochondrial cytochromes. ''Biochem. In ...h 3%(W/V) Sarkosyl and the extract was analyzed by high-performance liquid chromatography on a gel permeation column of TSK gel-G3000SW. This analytical system requi ...

...ted. The mutant was an aromatic prototroph and, by the criterion of column chromatography, appeared to have only a single functional species of shikimate kinase enzy == Useful Materials and Methods {{HelpIcon|Help:Literature}}== ...

...(2005) Proteome analysis of Escherichia coli using high-performance liquid chromatography and Fourier transform ion cyclotron resonance mass spectrometry. ''Proteomi ...fraction of an Escherichia coli cell lysate was prepared by size-exclusion chromatography and proteins were analyzed using C4 reversed phase (RP)-HPLC for pre-separa ...

...scherichia coli. Purification and characterization by Ni+ chelate affinity chromatography of the IIBCGlc subunit. ''FEBS Lett.'' '''324''':109-12 ...sisting of six adjacent histidines was purified by nickel chelate affinity chromatography. The protein was constitutively overexpressed from a high copy number plasm ...

...one, and then cleaved with thrombin. After separation by a cation-exchange chromatography step, the DNA-binding domain exhibited proper folding, as shown by proton N ...eins/genetics; Bacterial Proteins/isolation & purification; Base Sequence; Chromatography, Affinity; Cloning, Molecular; DNA, Bacterial/genetics; Escherichia coli/ge ...

...yridoxal phosphate as a mixed type inhibitor. As revealed by gel exclusion chromatography a minor fraction of the fusion protein formed a dimer, whereas the bulk amo == Useful Materials and Methods {{HelpIcon|Help:Literature}}== ...

...h citrate lyase multienzyme complex; it could be removed by anion-exchange chromatography with Q-Sepharose. The typical two configurations of citrate lyase, the 'sta == Useful Materials and Methods {{HelpIcon|Help:Literature}}== ...

...in can then be purified away from the MBP domain by repeating the affinity chromatography step. A prokaryotic (beta-galactosidase) and a eukaryotic (paramyosin) prot ...ette Transporters; Bacterial Proteins/genetics; Carrier Proteins/genetics; Chromatography, Affinity; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bac ...