nanS:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

nanS

Mnemonic
Synonyms

ECK4300, b4309, JW4272, yjhS[1], JW4272, yjhS

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

97.74 minutes, 97.74 minutes 

MG1655: 4535617..4534637
<gbrowseImage> name=NC_000913:4534637..4535617 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4517732..4516752
<gbrowseImage> name=NC_012967:4516752..4517732 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4473372..4474352
<gbrowseImage> name=NC_012759:4473372..4474352 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4542274..4541294
<gbrowseImage> name=NC_007779:4541294..4542274 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4637425..4636445
<gbrowseImage> name=NC_010473:4636445..4637425 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔyjhS (Keio:JW4272)

deletion

deletion

PMID:16738554[2]

Shigen

PMID:17012394[3] reports that yjhS is essential for growth on glycerol, using the Keio knockout strain; however, PMID:19749043[4] reports that yjhS is not required for growth on glycerol, using a newly constructed null mutant.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4272

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAACGCAATAATATCGCCCGA

Primer 2:CCtCTTTCGCGCCAAAACTGCAA

19H10

Kohara Phage

Genobase

PMID:3038334[6]

3F1

Kohara Phage

Genobase

PMID:3038334[6]

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[7]

est. P1 cotransduction: % [8]
Synonyms:zjh-901::Tn10, zjg-920::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[7]

est. P1 cotransduction: 5% [8]
Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4309

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5387

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YJHS

EcoCyc (EcoliWiki Page)

ECOCYC:G7919

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948835

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. Joyce, AR et al. (2006) Experimental and computational assessment of conditionally essential genes in Escherichia coli. J. Bacteriol. 188 8259-71 PubMed
  4. Steenbergen, SM et al. (2009) YjhS (NanS) is required for Escherichia coli to grow on 9-O-acetylated N-acetylneuraminic acid. J. Bacteriol. 191 7134-9 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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