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Standard Name	mhpE
Gene Synonym(s)	ECK0349, b0352, JW0343^[1], JW0343
Product Desc.	4-Hydroxy-2-oxovalerate aldolase; utilizes MHP^[2]
Product Synonyms(s)	4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase, class I^[1], B0352^[3]^[1], MhpE^[3]^[1] , ECK0349, JW0343, b0352
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mhpABCDFE^[3]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mhpE
Mnemonic	m-Hydroxyphenylpropionic acid
Synonyms	ECK0349, b0352, JW0343^[1], JW0343
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	8.04 minutes	MG1655: 373092..374105 <gbrowseImage> name=NC_000913:373092..374105 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 346424..347437 <gbrowseImage> name=NC_012967:346424..347437 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 275851..276864 <gbrowseImage> name=NC_012759:275851..276864 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 373092..374105 <gbrowseImage> name=NC_007779:373092..374105 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele

Nt change(s)

AA change(s)

Phenotype: Type

Phenotype: Description

Reference

Availability

Comments

mhpE(del) (Keio:JW0343)

deletion

PMID:16738554

Shigen

CGSC8533^[4]

mhpE721(del)::kan

PMID:16738554

CGSC:101862

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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0343	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAACGGTAAAAAACTTTATAT Primer 2:CCTTTGTTGTTGCGCAGATCCAG
8F10	Kohara Phage	Genobase	PMID:3038334
mhpC281::Tn10	Linked marker	CAG12080 = CGSC7333^[4]	est. P1 cotransduction: 94% ^[5] Synonyms:zah-281::Tn10
zai-3053::Tn10	Linked marker	CAG18091 = CGSC7336^[4]	est. P1 cotransduction: 46% ^[5] Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6205	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13292	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002819	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945012	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3077	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001209	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MhpE
Synonyms	4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase, class I^[1], B0352^[3]^[1], MhpE^[3]^[1] , ECK0349, JW0343, b0352
Product description	4-Hydroxy-2-oxovalerate aldolase; utilizes MHP^[2]
EC number (for enzymes)	4.1.3.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000891	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013785	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:16782065	IPI: Inferred from Physical Interaction	UniProtKB:P77580	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013785	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008701	4-hydroxy-2-oxovalerate aldolase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01656	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:16782065	IPI: Inferred from Physical Interaction	EcoliWiki:mhpF	F		complete
	GO:0008701	4-hydroxy-2-oxovalerate aldolase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017629	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008701	4-hydroxy-2-oxovalerate aldolase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.1.3.39	F	Seeded from EcoCyc (v14.0)	complete
Under review	GO:0008774	acetaldehyde dehydrogenase (acetylating) activity	PMID:6345511	IDA: Inferred from Direct Assay		F		complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016833	oxo-acid-lyase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012425	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019439	aromatic compound catabolic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01656	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019439	aromatic compound catabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012425	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019439	aromatic compound catabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017629	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019439	aromatic compound catabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0058	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	glpD	PMID:16606699	Experiment(s):EBI-1136343
Protein	prs	PMID:16606699	Experiment(s):EBI-1136343
Protein	mlc	PMID:16606699	Experiment(s):EBI-1136343
Protein	uspG	PMID:16606699	Experiment(s):EBI-1136343
Protein	fdnH	PMID:16606699	Experiment(s):EBI-1136343
Protein	rsuA	PMID:16606699	Experiment(s):EBI-1136343
Protein	rplN	PMID:16606699	Experiment(s):EBI-1136343
Protein	groL	PMID:16606699	Experiment(s):EBI-1136343
Protein	mhpF	PMID:19402753	MALDI(Z-score):20.031456
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNGKKLYISD VTLRDGMHAI RHQYSLENVR QIAKALDDAR VDSIEVAHGD GLQGSSFNYG FGAHSDLEWI EAAADVVKHA KIATLLLPGI GTIHDLKNAW QAGARVVRVA THCTEADVSA QHIQYARELG MDTVGFLMMS HMTTPENLAK QAKLMEGYGA TCIYVVDSGG AMNMSDIRDR FRALKAELKP ETQTGMHAHH NLSLGVANSI AAVEEGCDRI DASLAGMGAG AGNAPLEVFI AAADKLGWQH GTDLYALMDA ADDLVRPLQD RPVRVDRETL ALGYAGVYSS FLRHCETAAA RYGLSAVDIL VELGKRRMVG GQEDMIVDVA LDLRNNK
Length	337
Mol. Wt	36.47 kDa
pI	6.0 (calculated)
Extinction coefficient	31,400 - 31,900 (calc based on 10 Y, 3 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	14..247	PF00682 HMGL-like	PMID:19920124
Domain	271..336	PF07836 DmpG-like communication domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mhpE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128337	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945012	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001209	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P51020	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6205	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13292	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945012	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002819	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3077	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	1.758+/-0.135	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.007897335	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	15	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	18	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mhpABCDFE
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:373072..373112 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0352 (EcoliWiki Page)	NCBI GEO profiles for mhpE
microarray	GenExpDB:b0352 (EcoliWiki Page)	Summary of data for mhpE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mhpE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6205	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3077	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0352
EcoGene	EcoGene:EG13292	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002819	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001209	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Tetraodon nigroviridis	GSTENP00031359001 (score: 1.000; bootstrap: 100%) GSTENP00030684001 (score: 0.518)	From Inparanoid:20070104
E. coli O157	MHPE	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00682 HMGL-like
Superfamily (EcoliWiki Page)	SUPERFAMILY:51569
Panther (EcoliWiki Page)	PTHR10277:SF3
Superfamily (EcoliWiki Page)	SUPERFAMILY:89000
Pfam (EcoliWiki Page)	PF07836 DmpG-like communication domain
EcoCyc	EcoCyc:G6205	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13292	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002819	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3077	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001209	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

mhpE:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mhpE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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