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Product(s) |

Expression|

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Standard Name	lgt
Gene Synonym(s)	ECK2824, b2828, JW2796, umpA^[1], umpA
Product Desc.	phosphatidylglycerol-prolipoprotein diacylglyceryl transferase; a major membrane phospholipid^[2]^[3] Phosphatidylglycerol:prolipoprotein diacylglycerol transferase^[4]
Product Synonyms(s)	phosphatidylglycerol-prolipoprotein diacylglyceryl transferase^[1], B2828^[2]^[1], UmpA^[2]^[1], Lgt^[2]^[1] , ECK2824, JW2796, umpA, b2828
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): lgt-thyA^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	lgt
Mnemonic	(pro)Lipoprotein Glyceryl Transferase
Synonyms	ECK2824, b2828, JW2796, umpA^[1], umpA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	63.87 minutes	MG1655: 2964059..2963184 <gbrowseImage> name=NC_000913:2963184..2964059 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2860657..2859782 <gbrowseImage> name=NC_012967:2859782..2860657 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2850332..2851207 <gbrowseImage> name=NC_012759:2850332..2851207 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2964693..2963818 <gbrowseImage> name=NC_007779:2963818..2964693 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3057929..3057054 <gbrowseImage> name=NC_010473:3057054..3057929 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
lgtW25R		W25R	(in SK636, temperature-sensitive)			Strain variation; seeded from UniProt:P60955
lgt::Tn5KAN-I-SceI (FB20951)	Insertion at nt 552 in Plus orientation			PMID:15262929	E. coli Genome Project:FB20951	does not contain pKD46
lgt::Tn5KAN-I-SceI (FB20952)	Insertion at nt 552 in Plus orientation			PMID:15262929	E. coli Genome Project:FB20952	contains pKD46
lgtY26F		Y26F	No effect			seeded from UniProt:P60955
lgtH7Q		H7Q	No effect			seeded from UniProt:P60955
lgtH24Q		H24Q	No effect			seeded from UniProt:P60955
lgtG104S		G104S	(in SK634, temperature-sensitive)			Strain variation; seeded from UniProt:P60955
lgtL139F		L139F	(in SK635, temperature-sensitive)			Strain variation; seeded from UniProt:P60955
lgtH289Q		H289Q	No effect			seeded from UniProt:P60955
lgtY235S		Y235S	37% decrease in activity			seeded from UniProt:P60955
lgtY282F		Y282F	No effect			seeded from UniProt:P60955
lgtY76F		Y76F	No effect			seeded from UniProt:P60955
lgtH103Q,N		H103Q,N	Loss of activity			seeded from UniProt:P60955
lgtY62F		Y62F	No effect			seeded from UniProt:P60955
lgtY190F		Y190F	No effect			seeded from UniProt:P60955
lgtH196N,L		H196N,L	No effect			seeded from UniProt:P60955
lgtH196Q,R		H196Q,R	50% decrease in activity			seeded from UniProt:P60955
lgtY201F		Y201F	No effect			seeded from UniProt:P60955
lgtY235F,T		Y235F,T	Loss of activity			seeded from UniProt:P60955
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2796	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACCAGTAGCTATCTGCATTT Primer 2:CCGGAAACGTGTTGCTGTGGGCT
3G11	Kohara Phage	Genobase	PMID:3038334
5A10	Kohara Phage	Genobase	PMID:3038334
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[5]	est. P1 cotransduction: 65% ^[6]
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[5]	est. P1 cotransduction: % ^[6] Synonyms:zgd-210::Tn10, zgf-210::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12128	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12128	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002030	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947303	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2049	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009270	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Lgt
Synonyms	phosphatidylglycerol-prolipoprotein diacylglyceryl transferase^[1], B2828^[2]^[1], UmpA^[2]^[1], Lgt^[2]^[1] , ECK2824, JW2796, umpA, b2828
Product description	phosphatidylglycerol-prolipoprotein diacylglyceryl transferase; a major membrane phospholipid^[2]^[3] Phosphatidylglycerol:prolipoprotein diacylglycerol transferase^[4]
EC number (for enzymes)	2.4.99.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01147	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008961	phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01147	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009249	protein lipoylation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001640	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001640	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016757	transferase activity, transferring glycosyl groups	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001640	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042158	lipoprotein biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01147	P	Seeded from EcoCyc (v14.0)	complete
	GO:0042158	lipoprotein biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001640	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane	C-terminus localized in the cytoplasm with 5 predicted transmembrane domains	Daley et al. (2005) ^[7]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTSSYLHFPE FDPVIFSIGP VALHWYGLMY LVGFIFAMWL ATRRANRPGS GWTKNEVENL LYAGFLGVFL GGRIGYVLFY NFPQFMADPL YLFRVWDGGM SFHGGLIGVI VVMIIFARRT KRSFFQVSDF IAPLIPFGLG AGRLGNFING ELWGRVDPNF PFAMLFPGSR TEDILLLQTN PQWQSIFDTY GVLPRHPSQL YELLLEGVVL FIILNLYIRK PRPMGAVSGL FLIGYGAFRI IVEFFRQPDA QFTGAWVQYI SMGQILSIPM IVAGVIMMVW AYRRSPQQHV S
Length	291
Mol. Wt	33.108 kDa
pI	9.9 (calculated)
Extinction coefficient	63,370 (calc based on 13 Y, 8 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	10..284	PF01790 Prolipoprotein diacylglyceryl transferase		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lgt taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130732	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947303	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009270	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P60955	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12128	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12128	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947303	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002030	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2049	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1509	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	279	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	523	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
lgt-thyA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2964039..2964079 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2828 (EcoliWiki Page)	NCBI GEO profiles for lgt
microarray	GenExpDB:b2828 (EcoliWiki Page)	Summary of data for lgt from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2963964..2964262) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ09; Well:A7^[8]
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Notes

Accessions Related to lgt Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12128	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2049	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2828
EcoGene	EcoGene:EG12128	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002030	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009270	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000000896 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	LGT	From SHIGELLACYC
E. coli O157	LGT	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01790 Prolipoprotein diacylglyceryl transferase
EcoCyc	EcoCyc:EG12128	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12128	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002030	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2049	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009270	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

lgt:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to lgt Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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