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Expression|

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Standard Name	fucO
Gene Synonym(s)	ECK2794, b2799, JW2770^[1], JW2770
Product Desc.	propanediol oxidoreductase^[2]^[3]; Component of propanediol oxidoreductase^[2]^[3] L-1,2-Propanediol oxidoreductase^[4]
Product Synonyms(s)	L-1,2-propanediol oxidoreductase^[1], B2799^[2]^[1], FucO^[2]^[1] , ECK2794, JW2770, b2799
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fucAO^[2], OP00179
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fucO
Mnemonic	Fucose
Synonyms	ECK2794, b2799, JW2770^[1], JW2770
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	63.15 minutes	MG1655: 2931038..2929887 <gbrowseImage> name=NC_000913:2929887..2931038 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2821945..2820794 <gbrowseImage> name=NC_012967:2820794..2821945 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2817035..2818186 <gbrowseImage> name=NC_012759:2817035..2818186 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2931672..2930521 <gbrowseImage> name=NC_007779:2930521..2931672 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3024916..3023765 <gbrowseImage> name=NC_010473:3023765..3024916 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
fucO(del) (Keio:JW2770)	deletion	deletion			PMID:16738554	Shigen
fucO::Tn5KAN-I-SceI (FB20935)	Insertion at nt 365 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20935	does not contain pKD46
fucO::Tn5KAN-I-SceI (FB20936)	Insertion at nt 365 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20936	contains pKD46
fucA(del) fucO(del) rbsK(del)	deletion	deletion	in vivo	Triple mutant caused elimination of colony in vivo (mice), FIgure 2.	PMID:17709419[	Parental: MG1655
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2770	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATGGCTAACAGAATGATTCT Primer 2:CCCCAGGCGGTATGGTAAAGCTC
10B5	Kohara Phage	Genobase	PMID:3038334
8C5	Kohara Phage	Genobase	PMID:3038334
9A12	Kohara Phage	Genobase	PMID:3038334
cysI95::Tn10	Linked marker	CAG12173 = CGSC7425^[5]	est. P1 cotransduction: 15% ^[6] Synonyms:cysC95::Tn10
fucP3072::Tn10	Linked marker	CAG12079 = CGSC7427^[5]	est. P1 cotransduction: 92% ^[6] Synonyms:fuc-3072::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10351	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10351	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000345	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947273	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0347	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009177	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FucO
Synonyms	L-1,2-propanediol oxidoreductase^[1], B2799^[2]^[1], FucO^[2]^[1] , ECK2794, JW2770, b2799
Product description	propanediol oxidoreductase^[2]^[3]; Component of propanediol oxidoreductase^[2]^[3] L-1,2-Propanediol oxidoreductase^[4]
EC number (for enzymes)	1.1.1.77^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0008912	lactaldehyde reductase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.1.1.77	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001670	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018211	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of propanediol oxidoreductase	could be indirect
Protein	nadE		PMID:16606699	Experiment(s):EBI-1143992
Protein	yraN		PMID:16606699	Experiment(s):EBI-1143992
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMANRMILNE TAWFGRGAVG ALTDEVKRRG YQKALIVTDK TLVQCGVVAK VTDKMDAAGL AWAIYDGVVP NPTITVVKEG LGVFQNSGAD YLIAIGGGSP QDTCKAIGII SNNPEFADVR SLEGLSPTNK PSVPILAIPT TAGTAAEVTI NYVITDEEKR RKFVCVDPHD IPQVAFIDAD MMDGMPPALK AATGVDALTH AIEGYITRGA WALTDALHIK AIEIIAGALR GSVAGDKDAG EEMALGQYVA GMGFSNVGLG LVHGMAHPLG AFYNTPHGVA NAILLPHVMR YNADFTGEKY RDIARVMGVK VEGMSLEEAR NAAVEAVFAL NRDVGIPPHL RDVGVRKEDI PALAQAALDD VCTGGNPREA TLEDIVELYH TAW
Length	383
Mol. Wt	40.644 kDa
pI	5.0 (calculated)
Extinction coefficient	36,900 - 37,400 (calc based on 10 Y, 4 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	9..375	PF00465 Iron-containing alcohol dehydrogenase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fucO taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130706	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947273	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009177	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A9S1	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10351	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10351	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947273	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000345	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0347	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	22	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	24	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	10^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fucAO
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2931018..2931058 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2799 (EcoliWiki Page)	NCBI GEO profiles for fucO
microarray	GenExpDB:b2799 (EcoliWiki Page)	Summary of data for fucO from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to fucO Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10351	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0347	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2799
EcoGene	EcoGene:EG10351	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000345	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009177	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000018765 (score: 1.000; bootstrap: 100%) ENSAPMP00000023197 (score: 0.222) YP_026233 (score: 0.172)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000005874 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.144)	From Inparanoid:20070104
Caenorhabditis briggsae	YP_026233 (score: 0.255) WBGene00025759 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	YP_026233 (score: 0.250) WBGene00012608 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000010872 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.147)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000009349 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.153)	From Inparanoid:20070104
Danio rerio	YP_026233 (score: 0.216) ZDB-GENE-040426-2070 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-3017 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	YP_026233 (score: 1.000; bootstrap: 96%) DDB0219524 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	YP_026233 (score: 0.250) FBgn0017482 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	YP_026233 (score: 1.000; bootstrap: 87%) GA17444-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000022899 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.133)	From Inparanoid:20070104
Homo sapiens	ENSP00000276576 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.228)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000001187 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.205)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000009967 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.135)	From Inparanoid:20070104
Mus musculus	MGI:1923437 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.225)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000034755 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.300)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000009462 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.228)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000181441 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.200)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00008613001 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.240)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000050261 (score: 1.000; bootstrap: 100%) YP_026233 (score: 0.177)	From Inparanoid:20070104
Shigella flexneri	FUCO	From SHIGELLACYC
E. coli O157	FUCO	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00465 Iron-containing alcohol dehydrogenase
Superfamily (EcoliWiki Page)	SUPERFAMILY:56796
EcoCyc	EcoCyc:EG10351	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10351	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000345	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0347	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009177	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

fucO:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Alleles and Phenotypes

Notes

Genetic Interactions

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Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fucO Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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