fruA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

fruA

Gene Synonym(s)

ECK2160, b2167, JW2154, ptsF[1], ptsF

Product Desc.

FruA[2][3];

Component of EIIFru[2][3]

Fructose permease, PTS Enzyme IIBC component[4]

Product Synonyms(s)

fused fructose-specific PTS enzymes: IIBcomponent[1], IIC components[1], B2167[2][1], PtsF[2][1], FruA[2][1] , ECK2160, JW2154, ptsF, b2167

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): fruBKA[2], OP00220, fruBMH

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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C-terminal domain, last 200 residues, is member of LysE superfamily.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fruA

Mnemonic

Fructose

Synonyms

ECK2160, b2167, JW2154, ptsF[1], ptsF

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

48.66 minutes 

MG1655: 2259432..2257741
<gbrowseImage> name=NC_000913:2257741..2259432 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2212598..2210907
<gbrowseImage> name=NC_012967:2210907..2212598 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2150224..2151915
<gbrowseImage> name=NC_012759:2150224..2151915 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2264744..2263053
<gbrowseImage> name=NC_007779:2263053..2264744 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2350421..2348730
<gbrowseImage> name=NC_010473:2348730..2350421 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

fruA(del) (Keio:JW2154)

deletion

deletion

PMID:16738554

Shigen

CGSC9747[5]

fruA::Tn5KAN-2 (FB20664)

Insertion at nt 236 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20664

contains pKD46

fruA::Tn5KAN-2 (FB20665)

Insertion at nt 236 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20665

does not contain pKD46

fruA5

CGSC:6118

fruA3

CGSC:6126

fruA10

CGSC:8969

fruA0

CGSC:10763

fruA9

CGSC:12040

fruA4

CGSC:16086

fruA723(del)::kan

PMID:16738554

CGSC:101018

ptsF -

Resistant to

Resistant to sorbose

PMID:7028117

AS 1

table 1

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Notes

A homologous recombination using various digestive enzymes allowed the mutant allele, fruA2 to be derived.The initial transformant colonies were selected in the presence of both thiostrepton and apramycin. FruA is specifically involved in fructose catabolism. A nonpolar fruA mutation impairs growth on fructose and also leads to a constitutive low expression of the fru operon. Uptake and phosphorylation of frustose to fructose-1-phosphate by fruA is necessary to relieve repression by fruR[6].


Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2154

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAAACGCTGCTGATTATTGA

Primer 2:CCgGCTGCTTTCGCTACTGCGTC

4F2

Kohara Phage

Genobase

PMID:3038334

12C12

Kohara Phage

Genobase

PMID:3038334

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[5]

est. P1 cotransduction: % [7]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[5]

est. P1 cotransduction: 15% [7]
Synonyms:zeg-722, zei-722::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10336

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10336

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000330

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946672

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0332

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007172

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FruA

Synonyms

fused fructose-specific PTS enzymes: IIBcomponent[1], IIC components[1], B2167[2][1], PtsF[2][1], FruA[2][1] , ECK2160, JW2154, ptsF, b2167

Product description

FruA[2][3];

Component of EIIFru[2][3]

Fructose permease, PTS Enzyme IIBC component[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003352

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003353

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003501

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006327

F

Seeded from EcoCyc (v14.0)

complete

GO:0005351

sugar:hydrogen symporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013011

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008643

carbohydrate transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0762

P

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003352

F

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006327

F

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013011

F

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.1.69

F

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003352

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003353

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003501

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006327

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013011

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0598

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003352

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003353

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006327

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of EIIFru

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 9 predicted transmembrane domains

Daley et al. (2005) [8]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKTLLIIDAN LGQARAYMAK TLLGAAARKA KLEIIDNPND AEMAIVLGDS IPNDSALNGK
NVWLGDISRA VAHPELFLSE AKGHAKPYTA PVAATAPVAA SGPKRVVAVT ACPTGVAHTF
MAAEAIETEA KKRGWWVKVE TRGSVGAGNA ITPEEVAAAD LVIVAADIEV DLAKFAGKPM
YRTSTGLALK KTAQELDKAV AEATPYEPAG KAQTATTESK KESAGAYRHL LTGVSYMLPM
VVAGGLCIAL SFAFGIEAFK EPGTLAAALM QIGGGSAFAL MVPVLAGYIA FSIADRPGLT
PGLIGGMLAV STGSGFIGGI IAGFLAGYIA KLISTQLKLP QSMEALKPIL IIPLISSLVV
GLAMIYLIGK PVAGILEGLT HWLQTMGTAN AVLLGAILGG MMCTDMGGPV NKAAYAFGVG
LLSTQTYGPM AAIMAAGMVP PLAMGLATMV ARRKFDKAQQ EGGKAALVLG LCFISEGAIP
FAARDPMRVL PCCIVGGALT GAISMAIGAK LMAPHGGLFV LLIPGAITPV LGYLVAIIAG
TLVAGLAYAF LKRPEVDAVA KAA
Length

563

Mol. Wt

57.521 kDa

pI

9.0 (calculated)

Extinction coefficient

41,370 - 42,120 (calc based on 13 Y, 4 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

107..198

PF02302 PTS system, Lactose/Cellobiose specific IIB subunit

PMID:19920124

Domain

231..496

PF02378 Phosphotransferase system, EIIC

PMID:19920124

<motif_map/>

tmhmm.php?gene=fruA&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fruA taxon=12017 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130105

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946672

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007172

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P20966

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10336

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10336

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946672

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000330

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0332

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

359

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

179

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

154

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

fruBKA

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2259412..2259452 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2167 (EcoliWiki Page)

NCBI GEO profiles for fruA

microarray

GenExpDB:b2167 (EcoliWiki Page)

Summary of data for fruA from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to fruA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10336

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0332

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2167

EcoGene

EcoGene:EG10336

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000330

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007172

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

FRUA

From SHIGELLACYC

E. coli O157

FRUA

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02302 PTS system, Lactose/Cellobiose specific IIB subunit

Superfamily (EcoliWiki Page)

SUPERFAMILY:52794

Pfam (EcoliWiki Page)

PF02378 Phosphotransferase system, EIIC

EcoCyc

EcoCyc:EG10336

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10336

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000330

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0332

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007172

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Barrière, C et al. (2005) Fructose utilization in Lactococcus lactis as a model for low-GC gram-positive bacteria: its regulator, signal, and DNA-binding site. J. Bacteriol. 187 3752-61 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  8. Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

Categories

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