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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	cueO
Gene Synonym(s)	ECK0122, b0123, JW0119, yacK^[1], cuiD, yacK
Product Desc.	multicopper oxidase with role in copper homeostasis^[2]^[3] Multicopper oxidase (laccase), confers copper tolerance; oxidizes model substrate dimethoxyphenol; in vitro ferroxidase and phenoloxidase activities; regulated by copper-responsive CueR activator^[4]
Product Synonyms(s)	multicopper oxidase (laccase)^[1], B0123^[2]^[1], YacK^[2]^[1], CueO^[2]^[1], MCO^[2]^[1] , cuiD, ECK0122, JW0119, yacK, b0123
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cueO^[2]
Regulation/Activity
Quick Links
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Notes

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Verified Tat substrate: CueO has a Tat/Sec (Class II) 28 aa signal peptide (Tullman-Ercek, 2007).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cueO
Mnemonic	Cu efflux oxidase
Synonyms	ECK0122, b0123, JW0119, yacK^[1], cuiD, yacK
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	2.95 minutes	MG1655: 137083..138633 <gbrowseImage> name=NC_000913:137083..138633 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 139886..141436 <gbrowseImage> name=NC_012967:139886..141436 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 137082..138632 <gbrowseImage> name=NC_012759:137082..138632 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 137083..138633 <gbrowseImage> name=NC_007779:137083..138633 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 111187..112737 <gbrowseImage> name=NC_010473:111187..112737 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	137167	Edman degradation	PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
cueOCH500SR		CH500SR	Residual activity and loss of resistance to copper			seeded from UniProt:P36649
cueO(del) (Keio:JW0119)	deletion	deletion		PMID:16738554	Shigen
cueO6(del)::FLK2(lacZ,kan)				PMID:12142427	CGSC:85086
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0119	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCAACGTCGTGATTTCTTAAA Primer 2:CCTACCGTAAACCCTAACATCAT
4E11	Kohara Phage	Genobase	PMID:3038334
17C11	Kohara Phage	Genobase	PMID:3038334
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 8% ^[6] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 68% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12318	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12318	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947736	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002194	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2223	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000430	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CueO
Synonyms	multicopper oxidase (laccase)^[1], B0123^[2]^[1], YacK^[2]^[1], CueO^[2]^[1], MCO^[2]^[1] , cuiD, ECK0122, JW0119, yacK, b0123
Product description	multicopper oxidase with role in copper homeostasis^[2]^[3] Multicopper oxidase (laccase), confers copper tolerance; oxidizes model substrate dimethoxyphenol; in vitro ferroxidase and phenoloxidase activities; regulated by copper-responsive CueR activator^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0042597	periplasmic space	PMID:11527384	IDA: Inferred from Direct Assay		C		complete
	GO:0005507	copper ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002355	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005507	copper ion binding	PMID:11527384	IDA: Inferred from Direct Assay		F	4 coppers/ molecule	complete
	GO:0005507	copper ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011706	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005507	copper ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011707	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016722	oxidoreductase activity, oxidizing metal ions	PMID:11527384	IDA: Inferred from Direct Assay		F	ferroxidase activity	complete
	GO:0005507	copper ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0186	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001117	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002355	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011706	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011707	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	fur	PMID:16606699	Experiment(s):EBI-1135657
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1135657
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[3]
Periplasm	PMID:9298646	EchoLocation:cueO
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQRRDFLKYS VALGVASALP LWSRAVFAAE RPTLPIPDLL TTDARNRIQL TIGAGQSTFG GKTATTWGYN GNLLGPAVKL QRGKAVTVDI YNQLTEETTL HWHGLEVPGE VDGGPQGIIP PGGKRSVTLN VDQPAATCWF HPHQHGKTGR QVAMGLAGLV VIEDDEILKL MLPKQWGIDD VPVIVQDKKF SADGQIDYQL DVMTAAVGWF GDTLLTNGAI YPQHAAPRGW LRLRLLNGCN ARSLNFATSD NRPLYVIASD GGLLPEPVKV SELPVLMGER FEVLVEVNDN KPFDLVTLPV SQMGMAIAPF DKPHPVMRIQ PIAISASGAL PDTLSSLPAL PSLEGLTVRK LQLSMDPMLD MMGMQMLMEK YGDQAMAGMD HSQMMGHMGH GNMNHMNHGG KFDFHHANKI NGQAFDMNKP MFAAAKGQYE RWVISGVGDM MLHPFHIHGT QFRILSENGK PPAAHRAGWK DTVKVEGNVS EVLVKFNHDA PKEHAYMAHC HLLEHEDTGM MLGFTV
Length	516
Mol. Wt	56.556 kDa
pI	6.8 (calculated)
Extinction coefficient	62,910 - 63,285 (calc based on 9 Y, 9 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-28	UniProt Manual:Signal Peptides	UniProt:P36649
Domain	184..314	PF00394 Multicopper oxidase		PMID:19920124
Domain	363..516	PF07731 Multicopper oxidase		PMID:19920124
Domain	51..167	PF07732 Multicopper oxidase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cueO taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128116	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947736	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000430	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P36649	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12318	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12318	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947736	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002194	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2223	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	0.661+/-0.246	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
Protein	E. coli K-12 MG1655	610	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1987	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	601	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cueO
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:137063..137103 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0123 (EcoliWiki Page)	NCBI GEO profiles for cueO
microarray	GenExpDB:b0123 (EcoliWiki Page)	Summary of data for cueO from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (136951..137147) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ01; Well:B6^[7]
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Notes

Accessions Related to cueO Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12318	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2223	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0123
EcoGene	EcoGene:EG12318	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002194	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000430	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000000035 (score: 1.000; bootstrap: 68%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000008010 (score: 1.000; bootstrap: 100%) ENSAPMP00000006188 (score: 0.259) ENSAPMP00000021510 (score: 0.225) ENSAPMP00000008011 (score: 0.179) ENSAPMP00000028827 (score: 0.138) ENSAPMP00000016897 (score: 0.108)	From Inparanoid:20070104
Arabidopsis thaliana	AT1G71040 (score: 1.000; bootstrap: 70%) AT1G23010 (score: 0.781)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000002481 (score: 1.000; bootstrap: 100%) ENSCINP00000018618 (score: 0.249) ENSCINP00000018617 (score: 0.186) ENSCINP00000006845 (score: 0.169)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0039387 (score: 1.000; bootstrap: 100%) FBgn0052557 (score: 0.274) FBgn0032116 (score: 0.175) FBgn0050437 (score: 0.091)	From Inparanoid:20070104
Drosophila pseudoobscura	GA19259-PA (score: 1.000; bootstrap: 100%) GA16988-PA (score: 0.258) GA17667-PA (score: 0.192) GA15844-PA (score: 0.141)	From Inparanoid:20070104
Mus musculus	MGI:1332240 (score: 1.000; bootstrap: 100%) MGI:88476 (score: 0.423) MGI:2685355 (score: 0.399) MGI:88383 (score: 0.267) MGI:88382 (score: 0.246)	From Inparanoid:20070104
Oryza gramene	Q7F757 (score: 1.000; bootstrap: 64%) Q5ZE01 (score: 0.972) Q9AWU4 (score: 0.624) Q5ZDZ9 (score: 0.575) Q5ZE07 (score: 0.411) Q5ZE00 (score: 0.354)	From Inparanoid:20070104
Saccharomyces cerevisiae	YMR058W (score: 1.000; bootstrap: 100%) YFL041W (score: 0.406) YDR506C (score: 0.095)	From Inparanoid:20070104
Shigella flexneri	YACK	From SHIGELLACYC
E. coli O157	YACK	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00394 Multicopper oxidase
Superfamily (EcoliWiki Page)	SUPERFAMILY:49503
Superfamily (EcoliWiki Page)	SUPERFAMILY:49503
Superfamily (EcoliWiki Page)	SUPERFAMILY:49503
Pfam (EcoliWiki Page)	PF07731 Multicopper oxidase
Pfam (EcoliWiki Page)	PF07732 Multicopper oxidase
EcoCyc	EcoCyc:EG12318	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12318	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002194	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2223	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000430	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

cueO:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cueO Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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