\ PortEco: portal for E. coli research

We are still making minor changes. Feel free to use the browser, but be sure to check back later too

Browse and analyze data from Al Mamun et al. (Science 2012) Identity and Function of a Large Gene Network Underlying Mutagenic Repair of DNA Breaks 338:1344-1348

This dataset of 93 genes was constructed by screening E. coli for mutants deficient in mutagenic repair of DNA breaks under stress, followed by secondary screens for genes that participate in activation of stress responses. This study identifies a network of genes involved in stress-induced mutagenesis (the SIM Network). This data browser allows you to examine the classification of SIM network genes and explore the data from different assays on individual network genes. You can also download excel versions of the supplemental tables from the paper.

Views

nuoJ

Classification Electron transfer
Gene-product description NADH ubiquinone oxidoreductase subunit J; belongs to membrane fragment
References PMID:9593861[1]PMID:7607227[2]
SIM defect strength Strong
Previously known New

Mutagenesis Assays

Papillation assay defect
(fold-decrease in number of papillae compared with WT)
3.4 ± 0.4
Quantitative Lac assay defect
(mean fold decrease in mutation rate compared with WT)
16 ± 0.3
Chromosomal Tet assay defect
(mean fold decrease in TetR mutant frequency compared with WT)
Not tested [3]
Chromosomal Nal assay defect
(mean fold decrease in NalR mutant frequency compared with WT)
Not tested [3]

Screens for genes upstream of stress responses

σS upstream
(% decreases σS activity relative to WT)
Yes (35%)
Suppressed by mutation in arcB 24% [4]
Suppressed by mutation in arcA 31% [4]
Suppressed by mutation in rssB 18% [4]
Epistatic with rpoS mutation Predicted from nuoC
SDS-EDTA sensitive
E upstream candidate)
Yes
rpoHP3 promoter use defective
E upstream confirmed; % decreases σE activity relative to WT)
Yes (47.2%)
UV sensitive
(SOS response deficient candidate)
Resistant
Spontaneous SOS-deficient
Find more information about nuoJ via PortEco

References

  1. Friedrich, T (1998) The NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli. Biochim. Biophys. Acta 1364 134-46 PubMed
  2. Leif, H et al. (1995) Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli. Eur. J. Biochem. 230 538-48 PubMed
  3. 3.0 3.1 expected to be similar to nuoC
  4. 4.0 4.1 4.2 Not tested; predicted from nuoC

Help

Back to top

Coming soon...

Download data files

Back to top

Excel versions of supplemental tables: