TableEdit
PMID:20671182
You don't have sufficient rights on this wiki to edit tables. Perhaps you need to log in. Changes you make in the Table editor will not be saved back to the wiki
See Help for Help on this wiki. See the documentation for how to use the table editor
| Citation |
Taniguchi, Y, Choi, PJ, Li, GW, Chen, H, Babu, M, Hearn, J, Emili, A and Xie, XS (2010) Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329:533-8 |
|---|---|
| Abstract |
Protein and messenger RNA (mRNA) copy numbers vary from cell to cell in isogenic bacterial populations. However, these molecules often exist in low copy numbers and are difficult to detect in single cells. We carried out quantitative system-wide analyses of protein and mRNA expression in individual cells with single-molecule sensitivity using a newly constructed yellow fluorescent protein fusion library for Escherichia coli. We found that almost all protein number distributions can be described by the gamma distribution with two fitting parameters which, at low expression levels, have clear physical interpretations as the transcription rate and protein burst size. At high expression levels, the distributions are dominated by extrinsic noise. We found that a single cell's protein and mRNA copy numbers for any given gene are uncorrelated. |
| Links |
PubMed PMC2922915 Online version:10.1126/science.1188308 |
| Keywords |
Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/analysis; Escherichia coli Proteins/metabolism; Gene Expression; Gene Expression Profiling; Gene Library; In Situ Hybridization, Fluorescence; Luminescent Proteins; Microfluidic Analytical Techniques; Microscopy, Fluorescence; Protein Biosynthesis; Proteome/analysis; RNA Stability; RNA, Bacterial/analysis; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; RNA, Messenger/analysis; RNA, Messenger/genetics; Saccharomyces cerevisiae/chemistry; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Transcription, Genetic |
| public |
| Cancel |
