TableEdit
mhpC:Gene
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Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments | |
---|---|---|---|---|---|---|---|---|
public |
mhpC(del) (Keio:JW0340) |
deletion |
deletion |
PMID:16738554 |
||||
public |
mhpCR197K |
R197K |
40-fold and 5-fold decrease in catalytic activity and affinity, respectively |
seeded from UniProt:P77044 | ||||
public |
mhpCR197Q |
R197Q |
280-fold and 10-fold decrease in catalytic activity and affinity, respectively |
seeded from UniProt:P77044 | ||||
public |
mhpCS119A |
S119A |
Weakly active. 3-fold decrease in affinity. Fast ketonisation and slow C-C cleavage |
seeded from UniProt:P77044 | ||||
public |
mhpCC270A |
C270A |
2-fold decrease in catalytic activity and almost 2-fold increase in affinity |
seeded from UniProt:P77044 | ||||
public |
mhpCH272A |
H272A |
Weakly active, 1000-fold decrease in catalytic efficiency. Very slow ketonisation and C-C cleavage |
seeded from UniProt:P77044 | ||||
public |
mhpCW273G |
W273G |
10-fold and 20-fold decrease in catalytic activity and affinity, respectively |
seeded from UniProt:P77044 | ||||
public |
mhpCS119G |
S119G |
Weakly active. 3-fold decrease in affinity. Fast ketonisation and slow C-C cleavage |
seeded from UniProt:P77044 | ||||
public |
mhpCH123A |
H123A |
More than 2-fold decrease in catalytic efficiency and 3-fold increase affinity |
seeded from UniProt:P77044 | ||||
public |
mhpCS49A |
S49A |
2-fold decrease in catalytic efficiency and more than 5-fold increase in affinity for the natural substrate |
seeded from UniProt:P77044 | ||||
public |
mhpCN118A |
N118A |
200-fold decrease in catalytic activity and almost 2-fold increase in affinity |
seeded from UniProt:P77044 | ||||
public |
mhpCN118H |
N118H |
350-fold decrease in catalytic activity and almost 2-fold increase in affinity |
seeded from UniProt:P77044 | ||||
public |
mhpCF182D |
F182D |
100-fold decrease in catalytic activity |
seeded from UniProt:P77044 | ||||
public |
mhpCF182G |
F182G |
4-fold and 8-fold decrease in catalytic activity and affinity, respectively |
seeded from UniProt:P77044 | ||||
public |
mhpC281::Tn10 |
Insertion at 371,180 bp in MG1655 (NC_000913) |
adapted from Nichols et al.[2] |
Synonyms: zah-281::Tn10 | ||||
public |
mhpC281::Tn10 |
PMID:2540407 PMID:9829956 |
||||||
public |
ΔmhpC789::kan |
PMID:16738554 |
||||||
public |
MhpC H263A |
H263A |
Ser110, His263 and Asp235 compose the catalytic triad of MhpC, and mutation of His263 to Alanine causes a 10,000 fold reduction in Kcat for 2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase activity. |
PMID:15663941 |
||||
public |
MhpC Ser110A MhpC Ser110G |
Either: Ser110Ala or Ser110Gly |
Ser110, His263 and Asp235 compose the catalytic triad of MhpC, and mutation of Ser110 to Alanine or Glycine causes a 10,000 fold reduction in Kcat for 2-hydroxy-6-keto-nona-1,9-dioic acid 5,6-hydrolase activity. |
PMID:15663941 |
|
- ↑ 1.0 1.1 CGSC: The Coli Genetics Stock Center
- ↑ The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
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