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acnB:Gene

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

public

acnB(del) (Keio:JW0114)

deletion

deletion

PMID:16738554

Shigen

CGSC8396[1]

public

acnB(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938

public

acnB(del)::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938

public

acnB(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

public

acnB(del)::tetR

Internal deletion of acnB replaced with tetR gene.

Growth Phenotype

Cannot grow on acetate as the sole carbon and energy source with or without supplementation with glutamate.

PMID:9202458

public

acnB(del)::tetR

Internal deletion of acnB substituted with tetR.

Growth Phenotype

Grows poorly on minimal glucose medium unless the medium is supplemented with glutamate. The same phenotype is seen for growth on minimal medium with glycerol, lactate, pyruvate, or succinate as the carbon and energy source.

PMID:9202458

Mutants lacking citrate synthase (gltA) or isocitrate dehydrogenase (icd) are glutamate auxotrophs. The mutant lacking acnB doesn't have an absolutely requirement for glutamate because of the second aconitase activity (acnA).

public

acnB736(del)::kan

PMID:16738554

CGSC:99896


  1. CGSC: The Coli Genetics Stock Center



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