pGL2-Control - Revision history

Joncrz: /* Sources */

2011-08-29T21:46:04Z

Sources

← Older revision		Revision as of 21:46, 29 August 2011
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	==Sources==		==Sources==
	[http://www.promega.com/~~catalog~~/~~CatalogProducts.asp~~?~~catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DControl+Vector&description%5Ftext~~=pGL2~~%2DControl+Vector~~ Promega]		[http://www.promega.com/search-results/?q=pGL2-Control Promega]

	==References==		==References==

Joncrz: /* At a Glance */

2011-08-18T20:57:20Z

At a Glance

← Older revision		Revision as of 20:57, 18 August 2011
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	==At a Glance==		==At a Glance==
	[[~~Image~~:~~#pGL2~~-Control.~~gif~~\|thumb\|~~right\|350px~~\|pGL2-Control, ~~image provided by~~ [~~http://www.promega.com/figures/popup.asp?fn=0317va Promega~~]]]		[[File:PGL2-Control.jpg\|thumb\|450px\|pGL2-Control, Image made with [[PlasMapper]]]]
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	==Notes==		==Notes==
	The pGL2-Control Vector contains the SV40 promoter and enhancer sequences, resulting in strong luc expression in many types of mammalian cells. This plasmid is useful in monitoring transfection efficiency in general and is a convenient internal standard for promoter and enhancer activities expressed by pGL2 recombinants.		The pGL2-Control Vector contains the SV40 promoter and enhancer sequences, resulting in strong luc expression in many types of mammalian cells. This plasmid is useful in monitoring transfection efficiency in general and is a convenient internal standard for promoter and enhancer activities expressed by pGL2 recombinants.

Joncrz: Table edited by Joncrz via TableEdit

2011-08-18T20:55:28Z

Table edited by Joncrz via TableEdit

← Older revision		Revision as of 20:55, 18 August 2011
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	!align=left {{table heading style}} \|Link to Sequence		!align=left {{table heading style}} \|Link to Sequence
	\|\|		\|\|
	[http://www.~~promega~~.~~com~~/~~vectors~~/~~pgl2-c.txt Promega~~]		[http://www.ncbi.nlm.nih.gov/nuccore/27650340?report=fasta NCBI]
	\|-		\|-
	!align=left {{table heading style}} \|Features		!align=left {{table heading style}} \|Features
	\|\|		\|\|
	SV40 Promoter		SV40 Promoter<Br />
			Luciferase gene (luc)<Br />
	Luciferase gene (luc)		GLprimer2 binding site<Br />
			SV40 late poly(A) signal<Br />
	GLprimer2 binding site		SV40 Enhancer<Br />
			RVprimer4 binding site<Br />
	SV40 late poly(A) signal		ColE1-derived plasmid replication origin<Br />
			lactamase (Ampr) gene<Br />
	SV40 Enhancer		GLprimer1 binding site<Br />

	RVprimer4 binding site

	ColE1-derived plasmid replication origin

	lactamase (Ampr) gene

	GLprimer1 binding site

	\|-		\|-
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127.0.0.1: /Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

2010-06-17T18:36:41Z

/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

← Older revision		Revision as of 18:36, 17 June 2010
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	{\| id="B47176b1fac2c9" class=" tableEdit PlasmidTable"		{\| {{Prettytable}} width='30%' align=left id="B47176b1fac2c9" class=" tableEdit PlasmidTable"

	\|-		\|-
	!align=left \|Type		!align=left {{table heading style}} \|Type
	\|\|		\|\|
	Cloning		Cloning
	\|-		\|-
	!align=left \|Origin of Replication		!align=left {{table heading style}} \|Origin of Replication
	\|\|		\|\|
	f1		f1
	\|-		\|-
	!align=left \|Copy #		!align=left {{table heading style}} \|Copy #
	\|\|		\|\|

	\|-		\|-
	!align=left \|Markers		!align=left {{table heading style}} \|Markers
	\|\|		\|\|
	Ampicillin		Ampicillin
	\|-		\|-
	!align=left \|Link to Sequence		!align=left {{table heading style}} \|Link to Sequence
	\|\|		\|\|
	[http://www.promega.com/vectors/pgl2-c.txt Promega]		[http://www.promega.com/vectors/pgl2-c.txt Promega]
	\|-		\|-
	!align=left \|Features		!align=left {{table heading style}} \|Features
	\|\|		\|\|
	SV40 Promoter		SV40 Promoter
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	\|-		\|-
	!align=left \|Accession Number		!align=left {{table heading style}} \|Accession Number
	\|\|		\|\|
	[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]		[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]

127.0.0.1: Update TableEdit tables.

2010-01-09T02:38:33Z

Update TableEdit tables.

← Older revision		Revision as of 02:38, 9 January 2010
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	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Type		!align=left \|Type
	\|\|		\|\|
	Cloning		Cloning
	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Origin of Replication		!align=left \|Origin of Replication
	\|\|		\|\|
	f1		f1
	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Copy #		!align=left \|Copy #
	\|\|		\|\|

	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Markers		!align=left \|Markers
	\|\|		\|\|
	Ampicillin		Ampicillin
	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Link to Sequence		!align=left \|Link to Sequence
	\|\|		\|\|
	[http://www.promega.com/vectors/pgl2-c.txt Promega]		[http://www.promega.com/vectors/pgl2-c.txt Promega]
	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Features		!align=left \|Features
	\|\|		\|\|
	SV40 Promoter		SV40 Promoter
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	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Accession Number		!align=left \|Accession Number
	\|\|		\|\|
	[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]		[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]

	\|-class=~~'sortbottom'~~		\|- class="tableEdit_footer"
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	\|}		\|}
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127.0.0.1: Fixing references section.

2009-06-26T20:15:58Z

Fixing references section.

← Older revision		Revision as of 20:15, 26 June 2009
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	==References==		==References==
			{{BacktoTop}}
			{{RefHelp}}
			<references />
	<ref name='PMID:3323819'/>		<ref name='PMID:3323819'/>
	<ref name='PMID:2889129'/>		<ref name='PMID:2889129'/>
	~~<references/>~~

	==Sequence==		==Sequence==
	1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA		1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA

imported>Wikientrybot: Fix links due to TableEditLinks.php changes.

2009-02-19T21:29:42Z

Fix links due to TableEditLinks.php changes.

← Older revision		Revision as of 21:29, 19 February 2009
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	\|-		\|-
	!{{table heading style}} \|Type		!align=left {{table heading style}} \|Type
	\|		\|\|
	Cloning		Cloning
	\|-		\|-
	!{{table heading style}} \|Origin of Replication		!align=left {{table heading style}} \|Origin of Replication
	\|		\|\|
	f1		f1
	\|-		\|-
	!{{table heading style}} \|Copy #		!align=left {{table heading style}} \|Copy #
	\|		\|\|

	\|-		\|-
	!{{table heading style}} \|Markers		!align=left {{table heading style}} \|Markers
	\|		\|\|
	Ampicillin		Ampicillin
	\|-		\|-
	!{{table heading style}} \|Link to Sequence		!align=left {{table heading style}} \|Link to Sequence
	\|		\|\|
	[http://www.promega.com/vectors/pgl2-c.txt Promega]		[http://www.promega.com/vectors/pgl2-c.txt Promega]
	\|-		\|-
	!{{table heading style}} \|Features		!align=left {{table heading style}} \|Features
	\|		\|\|
	SV40 Promoter		SV40 Promoter

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	\|-		\|-
	!{{table heading style}} \|Accession Number		!align=left {{table heading style}} \|Accession Number
	\|		\|\|
	[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]		[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]
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127.0.0.1: Edited by wikibot

2008-04-30T12:12:59Z

Edited by wikibot

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	[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]		[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]
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Mattnat08: edited by Mattnat08 via TableEdit

2008-04-04T07:48:09Z

edited by Mattnat08 via TableEdit

← Older revision		Revision as of 07:48, 4 April 2008
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	!{{table heading style}} \|Features		!{{table heading style}} \|Features
	\|		\|
	~~Multiple cloning region~~		SV40 Promoter

	Luciferase gene (luc)		Luciferase gene (luc)

	GLprimer2 binding site		GLprimer2 binding site

	SV40 late poly(A) signal		SV40 late poly(A) signal

	SV40 Enhancer		SV40 Enhancer

	RVprimer4 binding site		RVprimer4 binding site

	ColE1-derived plasmid replication origin		ColE1-derived plasmid replication origin

	β-lactamase (Ampr) gene		lactamase (Ampr) gene

	GLprimer1 binding site		GLprimer1 binding site

Mattnat08: /* Notes */

2008-04-04T07:44:57Z

Notes

← Older revision		Revision as of 07:44, 4 April 2008
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	<Br clear='all'>		<Br clear='all'>
	==Notes==		==Notes==
	The pGL2-~~Enhancer~~ Vector contains ~~an SV40 enhancer, derived from~~ the SV40 ~~early~~ promoter, ~~located downstream~~ of ~~the luciferase gene after the poly(A)signal~~. This ~~aids~~ in ~~the verification of functional~~ promoter ~~elements because the presence of the~~ enhancer ~~in many cases will result in transcription of luc at higher levels~~.		The pGL2-Control Vector contains the SV40 promoter and enhancer sequences, resulting in strong luc expression in many types of mammalian cells. This plasmid is useful in monitoring transfection efficiency in general and is a convenient internal standard for promoter and enhancer activities expressed by pGL2 recombinants.

	==History {{HelpIcon\|Help:Plasmid}}==		==History {{HelpIcon\|Help:Plasmid}}==