pET-32 Ek/LIC - Revision history

Joncrz: /* At a Glance */

2011-08-16T18:12:04Z

At a Glance

← Older revision		Revision as of 18:12, 16 August 2011
Line 1:		Line 1:
	==At a Glance==		==At a Glance==
	[[~~Image~~:~~#PET32EkLIC~~.jpg~~\|right~~\|thumb\|~~300px~~\|Image ~~provided by~~ [~~http://www.emdbiosciences.com/docs/docs/PROT/TB161.pdf Novagen~~]]]		[[File:PET-32 Ek-LIC.jpg\|thumb\|450px\|pET-32 Ek/LIC, Image made with [[PlasMapper]]]]
	<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f-->		<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f-->
	<!--		<!--

Joncrz: /* Sequence {{HelpIcon|Help:Plasmid}} */

2011-08-16T18:09:08Z

Sequence {{HelpIcon|Help:Plasmid}}

@@ Line 68: / Line 68: @@
 {{PlasmidSeq}}
+<pre>
 [[Category:Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:lac Operator]][[Category:Trx Tag]][[Category:His Tag]][[Category:S Tag]][[Category:MCS]][[Category:lacI]]
 __NOTOC__

Joncrz: Table edited by Joncrz via TableEdit

2011-08-16T18:06:49Z

Table edited by Joncrz via TableEdit

← Older revision		Revision as of 18:06, 16 August 2011
Line 29:		Line 29:
	!align=left {{table heading style}} \|Link to Sequence		!align=left {{table heading style}} \|Link to Sequence
	\|\|		\|\|
			[http://www.emdchemicals.com/life-science-research/pet-32-ek-lic-vector-kit/EMD_BIO-69076/p_uuid?attachments=VSEQ Novagen]
	\|-		\|-
	!align=left {{table heading style}} \|Features		!align=left {{table heading style}} \|Features
	\|\|		\|\|
	T7 Promoter<br>		T7 Promoter<br />
	Trx Tag<br>		Trx Tag<br />
	His Tag<br>		His Tag<br />
	S Tag<br>		S Tag<br />
	MCS<br>		MCS<br />
	lacI coding sequence		lacI coding sequence
	\|-		\|-
Line 45:		Line 45:

	\|- class="tableEdit_footer"		\|- class="tableEdit_footer"
	\|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&page=0&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> \|\|		\|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&page=112851&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> \|\|
	\|}		\|}
	<!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--></protect>		<!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--></protect>

127.0.0.1: /Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

2010-06-17T18:35:08Z

/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

← Older revision		Revision as of 18:35, 17 June 2010
Line 8:		Line 8:
	*		*
	****************************************************************************************** -->		****************************************************************************************** -->
	{\| id="X470630554a72f" class=" tableEdit PlasmidTable"		{\| {{Prettytable}} width='30%' align=left id="X470630554a72f" class=" tableEdit PlasmidTable"

	\|-		\|-
	!align=left \|Type		!align=left {{table heading style}} \|Type
	\|\|		\|\|
	Cloning/Expression<ref name='PMID:7763371'/>		Cloning/Expression<ref name='PMID:7763371'/>
	\|-		\|-
	!align=left \|Origin of Replication		!align=left {{table heading style}} \|Origin of Replication
	\|\|		\|\|
	pBR322, f1		pBR322, f1
	\|-		\|-
	!align=left \|Copy #		!align=left {{table heading style}} \|Copy #
	\|\|		\|\|
	~40		~40
	\|-		\|-
	!align=left \|Markers		!align=left {{table heading style}} \|Markers
	\|\|		\|\|
	Ampicillin		Ampicillin
	\|-		\|-
	!align=left \|Link to Sequence		!align=left {{table heading style}} \|Link to Sequence
	\|\|		\|\|

	\|-		\|-
	!align=left \|Features		!align=left {{table heading style}} \|Features
	\|\|		\|\|
	T7 Promoter<br>		T7 Promoter<br>
Line 40:		Line 40:
	lacI coding sequence		lacI coding sequence
	\|-		\|-
	!align=left \|Accession Number		!align=left {{table heading style}} \|Accession Number
	\|\|		\|\|

127.0.0.1: Update TableEdit tables.

2010-01-09T02:37:02Z

Update TableEdit tables.

← Older revision		Revision as of 02:37, 9 January 2010
Line 8:		Line 8:
	*		*
	****************************************************************************************** -->		****************************************************************************************** -->
	{\|~~border~~="2" ~~cellpadding~~="4" ~~cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}} width='30%' align=left~~		{\| id="X470630554a72f" class=" tableEdit PlasmidTable"

	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Type		!align=left \|Type
	\|\|		\|\|
	Cloning/Expression<ref name='PMID:7763371'/>		Cloning/Expression<ref name='PMID:7763371'/>
	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Origin of Replication		!align=left \|Origin of Replication
	\|\|		\|\|
	pBR322, f1		pBR322, f1
	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Copy #		!align=left \|Copy #
	\|\|		\|\|
	~40		~40
	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Markers		!align=left \|Markers
	\|\|		\|\|
	Ampicillin		Ampicillin
	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Link to Sequence		!align=left \|Link to Sequence
	\|\|		\|\|

	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Features		!align=left \|Features
	\|\|		\|\|
	T7 Promoter<br>		T7 Promoter<br>
Line 40:		Line 40:
	lacI coding sequence		lacI coding sequence
	\|-		\|-
	!align=left ~~{{table heading style}}~~ \|Accession Number		!align=left \|Accession Number
	\|\|		\|\|


	\|-class=~~'sortbottom'~~		\|- class="tableEdit_footer"
	\|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&page=0&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table] \|\|		\|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&page=0&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> \|\|
	\|}		\|}
	<!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--></protect>		<!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--></protect>

127.0.0.1: Fixing references section.

2009-06-26T20:15:25Z

Fixing references section.

← Older revision		Revision as of 20:15, 26 June 2009
Line 61:		Line 61:

	==References==		==References==
			{{BacktoTop}}
	<references/>		{{RefHelp}}
			<references />

	==Sequence {{HelpIcon\|Help:Plasmid}}==		==Sequence {{HelpIcon\|Help:Plasmid}}==

imported>Wikientrybot: Fix links due to TableEditLinks.php changes.

2009-02-19T21:23:09Z

Fix links due to TableEditLinks.php changes.

← Older revision		Revision as of 21:23, 19 February 2009
Line 4:		Line 4:
	<!--		<!--
	******************************************************************************************		******************************************************************************************
	*		*
	* PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table.		* PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table.
	*		*
	****************************************************************************************** -->		****************************************************************************************** -->
	{\|{{Prettytable}} width='30%' align=left		{\|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}} width='30%' align=left

	\|-		\|-
	!{{table heading style}} \|Type		!align=left {{table heading style}} \|Type
	\|		\|\|
	Cloning/Expression<ref name='PMID:7763371'/>		Cloning/Expression<ref name='PMID:7763371'/>
	\|-		\|-
	!{{table heading style}} \|Origin of Replication		!align=left {{table heading style}} \|Origin of Replication
	\|		\|\|
	pBR322, f1		pBR322, f1
	\|-		\|-
	!{{table heading style}} \|Copy #		!align=left {{table heading style}} \|Copy #
	\|		\|\|
	~40		~40
	\|-		\|-
	!{{table heading style}} \|Markers		!align=left {{table heading style}} \|Markers
	\|		\|\|
	Ampicillin		Ampicillin
	\|-		\|-
	!{{table heading style}} \|Link to Sequence		!align=left {{table heading style}} \|Link to Sequence
	\|		\|\|

	\|-		\|-
	!{{table heading style}} \|Features		!align=left {{table heading style}} \|Features
	\|		\|\|
	T7 Promoter<br>		T7 Promoter<br>
	Trx Tag<br>		Trx Tag<br>
Line 39:		Line 40:
	lacI coding sequence		lacI coding sequence
	\|-		\|-
	!{{table heading style}} \|Accession Number		!align=left {{table heading style}} \|Accession Number
	\|		\|\|

	\|-
	~~\|colspan~~='2'\|[{{SERVER}}{{SCRIPTPATH}}~~/index.php~~?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&page=0&pagename={{FULLPAGENAMEE}} edit table]		\|-class='sortbottom'
			\|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&page=0&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table] \|\|
	\|}		\|}
	<!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--></protect>		<!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--></protect>

127.0.0.1: Edited by wikibot

2008-04-30T12:12:30Z

Edited by wikibot

← Older revision		Revision as of 12:12, 30 April 2008
Line 43:		Line 43:

	\|-		\|-
	\|colspan='2'\|[{{SERVER}}~~/colipedia~~/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&page=0&pagename={{FULLPAGENAMEE}} edit table]		\|colspan='2'\|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&page=0&pagename={{FULLPAGENAMEE}} edit table]
	\|}		\|}
	<!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--></protect>		<!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--></protect>

Miriam at 22:29, 26 November 2007

2007-11-26T22:29:30Z

← Older revision		Revision as of 22:29, 26 November 2007
Line 68:		Line 68:


	[[Category:Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Novagen Plasmids]]		[[Category:Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:lac Operator]][[Category:Trx Tag]][[Category:His Tag]][[Category:S Tag]][[Category:MCS]][[Category:lacI]]
	__NOTOC__		__NOTOC__

Miriam: /* At a Glance */

2007-11-26T22:28:07Z

At a Glance

← Older revision		Revision as of 22:28, 26 November 2007
Line 1:		Line 1:
	==At a Glance==		==At a Glance==
	[[Image:~~Pet-32eklic~~.~~JPG~~\|right\|thumb\|300px\|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB161.pdf Novagen]]]		[[Image:#PET32EkLIC.jpg\|right\|thumb\|300px\|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB161.pdf Novagen]]]
	<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f-->		<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f-->
	<!--		<!--
Line 48:		Line 48:
	<BR>		<BR>
	<Br clear='all'>		<Br clear='all'>

	==Notes==		==Notes==
	The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein<ref name='PMID:7763371'/>, and His•Tag and STag coding sequences that are cleavable with enterokinase (Ek) protease.		The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein<ref name='PMID:7763371'/>, and His•Tag and STag coding sequences that are cleavable with enterokinase (Ek) protease.