JimHu at 19:56, 8 October 2010

2010-10-08T19:56:39Z

← Older revision		Revision as of 19:56, 8 October 2010
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	lambda DE3 is a ~~prophage~~ that expresses T7 RNA polymerase under the control of a lacUV5 promoter.		lambda DE3 is a phage construct that expresses T7 RNA polymerase under the control of a lacUV5 promoter.

			Studier and Moffat<ref name='PMID:6708104'/> constructed lambda DE3 as one of a series of phage constructs where [[Phage_T7_1:Gene\|T7 gene 1]] was cloned into the lambda D69<ref name='PMID:6299896'/> a unique BamH1. D69 is:

			Bam1<sup>0</sup> srI(1-2)delta att<sup>+</sup> imm21 nin5 shn6

			In DE3, transcription of T7 gene 1 is rightward toward the int promoter. The BamH1 site where PlacUV5-T7 1 is inserted is in the int gene, so DE3 is an int mutant. Lysogens of DE3 are made using a helper phage of heterologous immunity. The int mutation should also prevent normal excision from lysogens.

	== References ==		== References ==
	{{RefHelp}}<references/>		{{RefHelp}}<references/>

JimHu: Created page with 'lambda DE3 is a prophage that expresses T7 RNA polymerase under the control of a lacUV5 promoter. == References == {{RefHelp}}'

2010-10-08T19:27:45Z

Created page with 'lambda DE3 is a prophage that expresses T7 RNA polymerase under the control of a lacUV5 promoter. == References == {{RefHelp}}<references/>'

New page

lambda DE3 is a prophage that expresses T7 RNA polymerase under the control of a lacUV5 promoter.

== References ==
{{RefHelp}}<references/>

lambda DE3 - Revision history

JimHu at 19:56, 8 October 2010

JimHu: Created page with 'lambda DE3 is a prophage that expresses T7 RNA polymerase under the control of a lacUV5 promoter. == References == {{RefHelp}}'