PMID:9843401 - Revision history

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-15T05:05:53Z

change PMID template to TableEdit version

← Older revision		Revision as of 05:05, 15 September 2013
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	!align=left \|Citation		!align=left \|Citation
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	'''~~No author listed~~''' (1998) Identification of two Escherichia coli pseudouridine synthases that show multisite specificity for 23S RNA. ''Biochemistry'' '''37''':15951-7		'''Huang, L, Ku, J, Pookanjanatavip, M, Gu, X, Wang, D, Greene, PJ and Santi, DV''' (1998) Identification of two Escherichia coli pseudouridine synthases that show multisite specificity for 23S RNA. ''Biochemistry'' '''37''':15951-7
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	!align=left \|Abstract		!align=left \|Abstract

imported>Wikientrybot: replace GO_table_reference with annotationlinks

2013-09-01T20:09:54Z

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 ==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-01T20:09:54Z

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-|{{TableHeadingColor}} |'''Abstract''' || Several putative Escherichia coli pseudouridine (Psi) synthases have been identified by iterative searching of genomic databases for ORFs homologous to known Psi synthases [Gustafsson et al. (1996) Nucleic Acids Res. 24, 3756-3762]. Of these, yceC and yfiI were proposed to encode Psi synthases which modify 23S rRNA. In the present work, yceC and yfiI were cloned and overexpressed in E. coli, and the encoded enzymes, YceC and YfiI, were purified to homogeneity. Both proteins converted Urd residues of rRNA to Psi, thus confirming their identities as Psi synthases. However, in in vitro experiments both enzymes extensively modified Urd residues of both 23S rRNA and 16S rRNA. Gene-disruption of yceCresulted in the absence of Psi modification at positions U955, 2504, and 2580 of 23S RNA, thus identifying these sites as in vivo targets for YceC. Likewise, yfiI disruption resulted in the absence of Psi modification at positions U1911, 1917, and possibly 1915 of 23S RNA. Disruption of yceC did not affect the growth under the conditions tested, whereas yfiI-disrupted cells showed a dramatic decrease in growth rate. Since YceC and YfiI hypermodify RNA in vitro, factors in addition to ribonucleotide sequence must contribute to the in vivo specificity of these enzymes.
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-|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9843401 PubMed]
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127.0.0.1: /Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

2010-06-17T21:35:41Z

/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

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	!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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127.0.0.1: Update TableEdit tables.

2010-01-09T05:16:30Z

Update TableEdit tables.

← Older revision		Revision as of 05:16, 9 January 2010
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	!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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	complete		complete

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imported>Wikientrybot: Fix links due to TableEditLinks.php changes.

2009-02-20T09:26:20Z

Fix links due to TableEditLinks.php changes.

← Older revision		Revision as of 09:26, 20 February 2009
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	~~[[Category:GO:0031118_!_rRNA_pseudouridine_synthesis]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0031118_!_rRNA_pseudouridine_synthesis~~ GO:0031118]		GO:0031118
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	rRNA pseudouridine synthesis		rRNA pseudouridine synthesis
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	~~[[Category:GO:0009982_!_pseudouridine_synthase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009982_!_pseudouridine_synthase_activity~~ GO:0009982]		GO:0009982
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	pseudouridine synthase activity		pseudouridine synthase activity

Siegele: Table edited by Siegele via TableEdit

2008-12-17T00:14:50Z

Table edited by Siegele via TableEdit

← Older revision		Revision as of 00:14, 17 December 2008
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	[[Category:GO:~~0009982_~~!~~_pseudouridine_synthase_activity~~]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:~~0009982_~~!~~_pseudouridine_synthase_activity~~ GO:~~0009982~~]		[[Category:GO:0031118_!_rRNA_pseudouridine_synthesis]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0031118_!_rRNA_pseudouridine_synthesis GO:0031118]
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	pseudouridine ~~synthase activity~~		rRNA pseudouridine synthesis
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	IDA: Inferred from Direct Assay		IDA: Inferred from Direct Assay
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	F		P
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	[[Category:GO:~~0031118_~~!~~_rRNA_pseudouridine_synthesis~~]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:~~0031118_~~!~~_rRNA_pseudouridine_synthesis~~ GO:~~0031118~~]		[[Category:GO:0009982_!_pseudouridine_synthase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009982_!_pseudouridine_synthase_activity GO:0009982]
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	~~rRNA~~ pseudouridine ~~synthesis~~		pseudouridine synthase activity
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	IDA: Inferred from Direct Assay		IDA: Inferred from Direct Assay
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	P		F
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			His-tagged RluC was purified and incubated with [5‘-32P]UMP-23S rRNA. After digestion with nuclease P1, [5‘-32P]ΨMP was observed by 2d-TLC (Fig. 2).
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	complete		complete

Siegele: Table edited by Siegele via TableEdit

2008-12-17T00:08:39Z

Table edited by Siegele via TableEdit

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Siegele: Table edited by Siegele via TableEdit

2008-12-17T00:03:35Z

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127.0.0.1: Edited by wikibot

2008-04-30T12:03:29Z

Edited by wikibot

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	[{{SERVER}}~~/colipedia~~/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.P46afa8694a2be&page=0&pagename={{FULLPAGENAMEE}}&type=0 Add GO annotations]		[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.P46afa8694a2be&page=0&pagename={{FULLPAGENAMEE}}&type=0 Add GO annotations]
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