PMID:7961467 - Revision history

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-15T04:52:25Z

change PMID template to TableEdit version

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	'''~~No author listed~~''' (1994) Transcription of the Escherichia coli recE gene from a promoter in Tn5 and IS50. ''J. Bacteriol.'' '''176''':7024-31		'''Clark, AJ, Satin, L and Chu, CC''' (1994) Transcription of the Escherichia coli recE gene from a promoter in Tn5 and IS50. ''J. Bacteriol.'' '''176''':7024-31
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imported>Wikientrybot: replace GO_table_reference with annotationlinks

2013-09-01T19:41:45Z

replace GO_table_reference with annotationlinks

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+<protect><annotationlinks/></protect>
 ==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-01T19:41:45Z

change PMID template to TableEdit version

← Older revision		Revision as of 19:41, 1 September 2013
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	~~<span style~~ = '~~font-size: large~~'> '~~''Clark, AJ, Satin, L and Chu, CC~~''' (1994) Transcription of the Escherichia coli recE gene from a promoter in Tn5 and IS50.''J. Bacteriol.'' '''176''':7024-31</~~span>~~		\|-
			!align=left \|Citation
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			'''No author listed''' (1994) Transcription of the Escherichia coli recE gene from a promoter in Tn5 and IS50. ''J. Bacteriol.'' '''176''':7024-31
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			!align=left \|Abstract
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			Six sbc::Tn5 insertions and one sbc::IS50 insertion, which cause recE expression in Escherichia coli, have been cloned, and their DNA sequences have been determined. The sites of insertion are found at three positions in a 10-bp region: 58, 63, and 68 bp upstream of recE. Primer extension experiments with the cloned Tn5 insertions demonstrate that recE transcripts start adjacent to the insertion elements of five of these mutations and both adjacent and one nucleotide within the insertion element for the sixth mutation. This supports the hypothesis that these mutations have inserted a promoter, and PCR analysis reveals an outward promoter within the distal 69 nucleotides of Tn5. Primer extension analysis of RNA from the uncloned Tn5 and IS50 mutants reveals three additional insertion sites close to the others. Because all the insertions lie in the spacer region between racC and recE, transcribed in sbcA6 and sbc-23 strains, we propose that these insertions be renamed recEs::Tn5 and recEs::IS50.
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			[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7961467 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC197076 PMC197076]

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	\|~~{{TableHeadingColor}}~~ \|~~'''Abstract'''~~ \|~~\| <protect>Six sbc::Tn5 insertions and one sbc::IS50 insertion~~, ~~which cause recE expression in~~ Escherichia coli, ~~have been cloned, and their DNA sequences have been determined. The sites of insertion are found at three positions in a 10-bp region: 58~~, 63, and 68 bp upstream of recE. Primer extension experiments with the cloned Tn5 insertions demonstrate that recE transcripts start adjacent to the insertion elements of five of these mutations and both adjacent and one nucleotide within the insertion element for the sixth mutation. This supports the hypothesis that these mutations have inserted a promoter, and PCR analysis reveals an outward promoter within the distal 69 nucleotides of Tn5. Primer extension analysis of RNA from the uncloned Tn5 and IS50 mutants reveals three additional insertion sites close to the others. Because all the insertions lie in the spacer region between racC and recE, ~~transcribed in sbcA6 and sbc-23 strains, we propose that these insertions be renamed recEs::Tn5 and recEs::IS50.</protect>~~		!align=left \|Keywords
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	\|{{~~TableHeadingColor~~}} ~~nowrap \|'''Links''' \|\| [http~~:~~//www~~.~~ncbi~~.~~nlm.nih.gov/entrez/query.fcgi?cmd~~=~~Retrieve~~&db=~~pubmed~~&~~dopt~~=~~Abstract~~&~~list_uids~~=~~7961467 PubMed~~]<br/>		Base Sequence; Cloning, Molecular; DNA Transposable Elements/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Exodeoxyribonucleases/genetics; Gene Expression; Genes, Bacterial/genetics; Molecular Sequence Data; Mutagenesis, Insertional; Polymerase Chain Reaction; Promoter Regions, Genetic/genetics; Sequence Analysis, DNA; Transcription, Genetic
	~~[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7961467 Full text]~~
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	==Significance {{HelpIcon\|Help:Literature}} ==		==Significance {{HelpIcon\|Help:Literature}} ==
	{{LitSignificance}}		{{LitSignificance}}

127.0.0.1: /Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

2010-06-17T21:09:45Z

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	!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

127.0.0.1: Update TableEdit tables.

2010-01-09T04:55:38Z

Update TableEdit tables.

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imported>Wikientrybot: Fix links due to TableEditLinks.php changes.

2009-02-20T06:24:54Z

Fix links due to TableEditLinks.php changes.

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Joseph at 03:04, 23 May 2008

2008-05-23T03:04:08Z

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	\|{{TableHeadingColor}} nowrap \|'''Links''' \|\| [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7961467 PubMed]<br/>		\|{{TableHeadingColor}} nowrap \|'''Links''' \|\| [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7961467 PubMed]<br/>
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Joseph: New PMID: Page!

2008-05-23T03:02:52Z

New PMID: Page!

New page

{{RightTOC}}

<span style = 'font-size: large'> '''Clark, AJ, Satin, L and Chu, CC''' (1994) Transcription of the Escherichia coli recE gene from a promoter in Tn5 and IS50.''J. Bacteriol.'' '''176''':7024-31</span>

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Six sbc::Tn5 insertions and one sbc::IS50 insertion, which cause recE expression in Escherichia coli, have been cloned, and their DNA sequences have been determined. The sites of insertion are found at three positions in a 10-bp region: 58, 63, and 68 bp upstream of recE. Primer extension experiments with the cloned Tn5 insertions demonstrate that recE transcripts start adjacent to the insertion elements of five of these mutations and both adjacent and one nucleotide within the insertion element for the sixth mutation. This supports the hypothesis that these mutations have inserted a promoter, and PCR analysis reveals an outward promoter within the distal 69 nucleotides of Tn5. Primer extension analysis of RNA from the uncloned Tn5 and IS50 mutants reveals three additional insertion sites close to the others. Because all the insertions lie in the spacer region between racC and recE, transcribed in sbcA6 and sbc-23 strains, we propose that these insertions be renamed recEs::Tn5 and recEs::IS50.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7961467 PubMed]<br/>
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='242144'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>