5.9.72.48: Fill PMID: Page!

2016-07-07T02:05:09Z

Fill PMID: Page!

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			!align=left \|Citation
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			'''Brockes, JP, Brown, PR and Murray, K''' (1972) The deoxyribonucleic acid modification enzyme of bacteriophage P1. ''Biochem. J.'' '''127''':1-10
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			!align=left \|Abstract
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			The bacteriophage P1 modification enzyme, assayed by the specific methylation of unmodified bacteriophage 82 DNA, has been purified 500-fold from a bacteriophage P1 lysogen of Escherichia coli. The enzyme catalyses the incorporation of approximately 20-24 methyl groups per bacteriophage 82 DNA molecule. The sole product of methylation is 6-methylaminopurine. Methylation of unmodified bacteriophage DNA confers protection against a challenge by purified bacteriophage P1 restriction enzyme. The pH optimum is 6.0-6.25: the apparent K(m) for S-adenosyl-l-methionine is 5x10(-6)m.
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			!align=left \|Links
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			[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=5073742 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1178554 PMC1178554]

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			!align=left \|Keywords
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			Ammonium Sulfate; Catalase; Centrifugation, Zonal; Chromatography, DEAE-Cellulose; Coliphages/enzymology; DNA, Viral; Dialysis; Hydrogen-Ion Concentration; Hydrolysis; Methylation; Methyltransferases/isolation & purification; Morpholines; Purines; S-Adenosylmethionine; Sulfonic Acids; Tritium

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			==Significance {{HelpIcon\|Help:Literature}} ==
			{{LitSignificance}}

			== Useful Materials and Methods {{HelpIcon\|Help:Literature}}==
			{{LitMaterials}}

			==Annotations {{HelpIcon\|Help:Literature}}==
			<annotationlinks/>

			==EcoliWiki Links {{HelpIcon\|Help:Literature}} ==
			{{LitLinks}}

			==See also==

			==References==
			{{RefHelp}}
			<references/>

5.9.72.48: New PMID: Page!

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