PMID:2477358 - Revision history

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-15T04:44:07Z

change PMID template to TableEdit version

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	'''~~No author listed~~''' (1989) Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12. ''J. Bacteriol.'' '''171''':5479-86		'''Claverie-Martin, F, Diaz-Torres, MR, Yancey, SD and Kushner, SR''' (1989) Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12. ''J. Bacteriol.'' '''171''':5479-86
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imported>Wikientrybot: replace GO_table_reference with annotationlinks

2013-09-01T19:17:31Z

replace GO_table_reference with annotationlinks

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 ==Annotations {{HelpIcon|Help:Literature}}==
-<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.K4836377c7fb4f-->
+<protect><annotationlinks/></protect>
 ==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-01T19:17:31Z

change PMID template to TableEdit version

← Older revision		Revision as of 19:17, 1 September 2013
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	~~<span style~~ = '~~font-size: large~~'> '~~''Claverie-Martin, F, Diaz-Torres, MR, Yancey, SD and Kushner, SR~~''' (1989) Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12.''J. Bacteriol.'' '''171''':5479-86</~~span>~~		\|-
			!align=left \|Citation
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			'''No author listed''' (1989) Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12. ''J. Bacteriol.'' '''171''':5479-86
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			!align=left \|Abstract
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			A temperature-sensitive mutation in the ams gene of Escherichia coli causes an increase in the chemical half-life of pulse-labeled RNA at the nonpermissive temperature. Using lambda clones containing DNA fragments from the 23- to 24-min region on the E. coli chromosome, we have isolated a 5.8-kilobase DNA fragment which, when present in a low-copy-number plasmid, complements the conditional lethality and increased mRNA stability associated with the ams-1 mutation. The approximate initiation site and the direction of transcription of the ams gene were determined from the size of truncated polypeptides produced by Tn1000 insertions and Bal 31 deletions. Overexpression of the ams locus by using a T7 RNA polymerase-promoter system permitted the identification of an ams-encoded polypeptide of 110 kilodaltons.
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			[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2477358 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC210387 PMC210387]

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	\|~~{{TableHeadingColor}}~~ \|~~'''Abstract'''~~ \|~~\| <protect>A temperature-sensitive mutation in the ams gene of~~ Escherichia coli ~~causes an increase in the chemical half-life of pulse-labeled~~ RNA ~~at the nonpermissive temperature. Using lambda clones containing DNA fragments from the 23- to 24-min region on the E. coli chromosome~~, ~~we have isolated a 5.8-kilobase DNA fragment which~~, when present in a low-copy-number plasmid, complements the conditional lethality and increased mRNA stability associated with the ams-1 mutation. The approximate initiation site and the direction of transcription of the ams gene were determined from the size of truncated polypeptides produced by Tn1000 insertions and Bal 31 deletions. Overexpression of the ams locus by using a T7 RNA polymerase-promoter system permitted the identification of an ams-encoded polypeptide of 110 kilodaltons.</~~protect>~~		!align=left \|Keywords
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	\|{{~~TableHeadingColor~~}} ~~nowrap \|'''Links''' \|\| [http~~:~~//www~~.~~ncbi~~.~~nlm.nih.gov/entrez/query.fcgi?cmd~~=~~Retrieve~~&db=~~pubmed~~&~~dopt~~=~~Abstract~~&~~list_uids~~=~~2477358 PubMed~~]<br/>		Blotting, Southern; Cloning, Molecular; DNA Mutational Analysis; DNA Transposable Elements; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial; Genetic Complementation Test; Molecular Weight; Nucleic Acid Hybridization; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; Restriction Mapping; Ribonucleases/genetics
	~~[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2477358 Full text]~~
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	==Significance {{HelpIcon\|Help:Literature}} ==		==Significance {{HelpIcon\|Help:Literature}} ==
	{{LitSignificance}}		{{LitSignificance}}

127.0.0.1: /Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

2010-06-17T20:49:28Z

/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

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	!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

127.0.0.1: Update TableEdit tables.

2010-01-09T04:39:29Z

Update TableEdit tables.

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	!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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imported>Wikientrybot: Fix links due to TableEditLinks.php changes.

2009-02-20T04:47:33Z

Fix links due to TableEditLinks.php changes.

← Older revision		Revision as of 04:47, 20 February 2009
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	!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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Joseph at 03:18, 23 May 2008

2008-05-23T03:18:46Z

← Older revision		Revision as of 03:18, 23 May 2008
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	\|{{TableHeadingColor}} nowrap \|'''Links''' \|\| [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2477358 PubMed]<br/>		\|{{TableHeadingColor}} nowrap \|'''Links''' \|\| [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2477358 PubMed]<br/>
			[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2477358 Full text]
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Joseph: New PMID: Page!

2008-05-23T03:18:22Z

New PMID: Page!

New page

{{RightTOC}}

<span style = 'font-size: large'> '''Claverie-Martin, F, Diaz-Torres, MR, Yancey, SD and Kushner, SR''' (1989) Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12.''J. Bacteriol.'' '''171''':5479-86</span>

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>A temperature-sensitive mutation in the ams gene of Escherichia coli causes an increase in the chemical half-life of pulse-labeled RNA at the nonpermissive temperature. Using lambda clones containing DNA fragments from the 23- to 24-min region on the E. coli chromosome, we have isolated a 5.8-kilobase DNA fragment which, when present in a low-copy-number plasmid, complements the conditional lethality and increased mRNA stability associated with the ams-1 mutation. The approximate initiation site and the direction of transcription of the ams gene were determined from the size of truncated polypeptides produced by Tn1000 insertions and Bal 31 deletions. Overexpression of the ams locus by using a T7 RNA polymerase-promoter system permitted the identification of an ams-encoded polypeptide of 110 kilodaltons.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2477358 PubMed]<br/>
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='242149'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.K4836377c7fb4f&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>