PMID:22684885 - Revision history

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-15T04:43:08Z

change PMID template to TableEdit version

← Older revision		Revision as of 04:43, 15 September 2013
Line 12:		Line 12:
	!align=left \|Citation		!align=left \|Citation
	\|\|		\|\|
	'''Wang, J , Zhang, Y , Chen, Y , Lin, M and Lin, Z ''' (2012) Global regulator engineering significantly improved Escherichia coli tolerances toward inhibitors of lignocellulosic hydrolysates. ''Biotechnol. Bioeng.'' '''109''':3133-42		'''Wang, J, Zhang, Y, Chen, Y, Lin, M and Lin, Z''' (2012) Global regulator engineering significantly improved Escherichia coli tolerances toward inhibitors of lignocellulosic hydrolysates. ''Biotechnol. Bioeng.'' '''109''':3133-42
	\|-		\|-
	!align=left \|Abstract		!align=left \|Abstract
Line 31:		Line 31:
	\|}		\|}
	<!--box uid=d41d8cd98f00b204e9800998ecf8427e.182973.E5150bd0618527-->		<!--box uid=d41d8cd98f00b204e9800998ecf8427e.182973.E5150bd0618527-->

	==Significance {{HelpIcon\|Help:Literature}} ==		==Significance {{HelpIcon\|Help:Literature}} ==
	{{LitSignificance}}		{{LitSignificance}}

imported>Wikientrybot: replace GO_table_reference with annotationlinks

2013-09-01T19:16:20Z

replace GO_table_reference with annotationlinks

@@ Line 39: / Line 39: @@
 ==Annotations {{HelpIcon|Help:Literature}}==
-<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.182973.C5150bd0681f76-->
+<protect><annotationlinks/></protect>
 ==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

Siegele at 22:24, 22 May 2013

2013-05-22T22:24:38Z

← Older revision		Revision as of 22:24, 22 May 2013
Line 63:		Line 63:
	{{RefHelp}}		{{RefHelp}}
	<references/>		<references/>

			[[Category:Expression data]]

165.91.108.111: Fill PMID: Page!

2013-03-25T21:09:26Z

Fill PMID: Page!

← Older revision	Revision as of 21:09, 25 March 2013
Line 1:	Line 1:
		{{RightTOC}}
		<!--box uid=d41d8cd98f00b204e9800998ecf8427e.182973.E5150bd0618527-->
		<!--
		******************************************************************************************
		*
		* PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table.
		*
		****************************************************************************************** -->
		{\| id="E5150bd0618527" class=" tableEdit PMID_info_table"

		\|-
		!align=left \|Citation
		\|\|
		'''Wang, J , Zhang, Y , Chen, Y , Lin, M and Lin, Z ''' (2012) Global regulator engineering significantly improved Escherichia coli tolerances toward inhibitors of lignocellulosic hydrolysates. ''Biotechnol. Bioeng.'' '''109''':3133-42
		\|-
		!align=left \|Abstract
		\|\|
		Lignocellulosic biomass is regarded as the most viable source of feedstock for industrial biorefinery, but the harmful inhibitors generated from the indispensable pretreatments prior to fermentation remain a daunting technical hurdle. Using an exogenous regulator, irrE, from the radiation-resistant Deinococcus radiodurans, we previously showed that a novel global regulator engineering (GRE) approach significantly enhanced tolerances of Escherichia coli to alcohol and acetate stresses. In this work, an irrE library was subjected to selection under various stresses of furfural, a typical hydrolysate inhibitor. Three furfural tolerant irrE mutants including F1-37 and F2-1 were successfully obtained. The cells containing these mutants reached OD(600) levels of 4- to 16-fold of that for the pMD18T cells in growth assay under 0.2% (v/v) furfural stress. The cells containing irrE F1-37 and F2-1 also showed considerably reduced intracellular oxygen species (ROS) levels under furfural stress. Moreover, these two irrE mutants were subsequently found to confer significant cross tolerances to two other most common inhibitors, 5-hydroxymethyl-2-furaldehyde (HMF), vanillin, as well as real lignocellulosic hydrolysates. When evaluated in Luria-Bertani (LB) medium supplemented with corn stover cellulosic hydrolysate (prepared with a solid loading of 30%), the cells containing the mutants exhibited lag phases markedly shortened by 24-44 h in comparison with the control cells. This work thus presents a promising step forward to resolve the inhibitor problem for E. coli. From the view of synthetic biology, irrE can be considered as an evolvable "part" for various stresses. Furthermore, this GRE approach can be extended to exploit other exogenous global regulators from extremophiles, and the native counterparts in E. coli, for eliciting industrially useful phenotypes.
		\|-
		!align=left \|Links
		\|\|
		[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=22684885 PubMed]
		Online version:[http://dx.doi.org/10.1002/bit.24574 10.1002/bit.24574]
		\|-
		!align=left \|Keywords
		\|\|
		Biomass; Bioreactors/microbiology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/physiology; Furaldehyde/chemistry; Furaldehyde/metabolism; Genetic Engineering/methods; Lignin/antagonists & inhibitors; Lignin/metabolism; Models, Molecular; Mutation; Polysaccharides/metabolism; Reactive Oxygen Species/metabolism; Sequence Alignment

		\|- class="tableEdit_footer"
		\|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182973.E5150bd0618527&page=182973&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> \|\|
		\|}
		<!--box uid=d41d8cd98f00b204e9800998ecf8427e.182973.E5150bd0618527-->

		==Significance {{HelpIcon\|Help:Literature}} ==
		{{LitSignificance}}

		== Useful Materials and Methods {{HelpIcon\|Help:Literature}}==
		{{LitMaterials}}

		==Annotations {{HelpIcon\|Help:Literature}}==
		<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.182973.C5150bd0681f76-->
		<!--
		******************************************************************************************
		*
		* PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table.
		*
		****************************************************************************************** -->
		{\| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C5150bd0681f76" class=" tableEdit GO_table_reference dataTable"
		\|- {{table heading style}}
		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

		\|- class="tableEdit_footer"
		\|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182973.C5150bd0681f76&page=182973&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table]</span> \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
		\|}
		<!--box uid=d41d8cd98f00b204e9800998ecf8427e.182973.C5150bd0681f76--></protect>

		==EcoliWiki Links {{HelpIcon\|Help:Literature}} ==
		{{LitLinks}}

		==See also==

		==References==
		{{RefHelp}}
		<references/>

165.91.108.111: New PMID: Page!

2013-03-25T21:09:26Z

New PMID: Page!

New page