PMID:16738554 - Revision history

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-15T04:30:16Z

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	'''~~No author listed~~''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008		'''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
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	!align=left \|Abstract		!align=left \|Abstract

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-01T18:53:30Z

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	<span style = 'font-size: large'> '''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.''Mol. Syst. Biol.'' '''2''':2006.0008</span>

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	\|~~{{TableHeadingColor}}~~ \|'''~~Abstract~~''' \|\| We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).		!align=left \|Citation
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			'''No author listed''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. ''Mol. Syst. Biol.'' '''2''':2006.0008
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			!align=left \|Abstract
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			We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
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			[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681482 PMC1681482]
			Online version:[http://dx.doi.org/10.1038/msb4100050 10.1038/msb4100050]
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	* [~~http~~:~~//www~~.~~ncbi~~.~~nlm.nih.gov/entrez/query.fcgi?cmd~~=~~Retrieve~~&db=~~pubmed~~&~~dopt~~=~~Abstract~~&~~list_uids~~=~~16738554 PubMed~~]		\|\|
	* [http:/~~/www.nature.com/msb/journal/v2/n1/full/msb4100050.html Full Text]~~		Escherichia coli/genetics; Gene Deletion; Internet; Mutation; Organisms, Genetically Modified

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127.0.0.1: Update TableEdit tables.

2010-01-09T04:26:44Z

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JimHu at 22:17, 19 January 2009

2009-01-19T22:17:44Z

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			* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed]
			* [http://www.nature.com/msb/journal/v2/n1/full/msb4100050.html Full Text]
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127.0.0.1: Edited by wikibot

2008-04-30T12:00:49Z

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127.0.0.1: Edited by wikibot

2008-04-25T22:03:50Z

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	==EcoliWiki Links {{HelpIcon\|Help:Literature}} ==		==EcoliWiki Links {{HelpIcon\|Help:Literature}} ==

Gwen: /* Significance {{HelpIcon|Help:Literature}} */

2008-02-03T05:34:16Z

Significance {{HelpIcon|Help:Literature}}

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	== Useful Materials and Methods {{HelpIcon\|Help:Literature}}==		== Useful Materials and Methods {{HelpIcon\|Help:Literature}}==

Azweifel: /* Significance {{HelpIcon|Help:Literature}} */

2007-08-10T21:14:52Z

Significance {{HelpIcon|Help:Literature}}

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	== Useful Materials and Methods {{HelpIcon\|Help:Literature}}==		== Useful Materials and Methods {{HelpIcon\|Help:Literature}}==

Azweifel: /* Significance {{HelpIcon|Help:Literature}} */

2007-08-10T21:14:07Z

Significance {{HelpIcon|Help:Literature}}

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	==Significance {{HelpIcon\|Help:Literature}} ==		==Significance {{HelpIcon\|Help:Literature}} ==
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			blah

	== Useful Materials and Methods {{HelpIcon\|Help:Literature}}==		== Useful Materials and Methods {{HelpIcon\|Help:Literature}}==

165.91.8.142: New PMID: Page!

2007-07-28T18:10:30Z

New PMID: Page!

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<span style = 'font-size: large'> '''Baba, T, Ara, T, Hasegawa, M, Takai, Y, Okumura, Y, Baba, M, Datsenko, KA, Tomita, M, Wanner, BL and Mori, H''' (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.''Mol. Syst. Biol.'' '''2''':2006.0008</span>

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
</noinclude>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16738554 PubMed]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>
[{{SERVER}}/colipedia/index.php/Special:TableEdit?id=63ce1649aab8b3180235b0af7618f667.0.B46ab8696aabcb&page=0&pagename={{FULLPAGENAMEE}}&type=0 Add GO annotations]
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
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<references/>