PMID:16632468 - Revision history

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-15T04:30:11Z

change PMID template to TableEdit version

← Older revision		Revision as of 04:30, 15 September 2013
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	!align=left \|Citation		!align=left \|Citation
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	'''~~No author listed~~''' (2006) The AddAB helicase/nuclease forms a stable complex with its cognate chi sequence during translocation. ''J. Biol. Chem.'' '''281''':18610-7		'''Chédin, F, Handa, N, Dillingham, MS and Kowalczykowski, SC''' (2006) The AddAB helicase/nuclease forms a stable complex with its cognate chi sequence during translocation. ''J. Biol. Chem.'' '''281''':18610-7
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	!align=left \|Abstract		!align=left \|Abstract

imported>Wikientrybot: replace GO_table_reference with annotationlinks

2013-09-01T18:53:08Z

replace GO_table_reference with annotationlinks

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 ==Annotations {{HelpIcon|Help:Literature}}==
-<!--box uid=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4-->
+<annotationlinks/>
 ==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-01T18:53:08Z

change PMID template to TableEdit version

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 {{RightTOC}}
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-|{{TableHeadingColor}} |'''Abstract''' || The Bacillus subtilis AddAB enzyme possesses ATP-dependent helicase and nuclease activities, which result in the unwinding and degradation of double-stranded DNA (dsDNA) upon translocation. Similar to its functional counterpart, the Escherichia coli RecBCD enzyme, it also recognizes and responds to a specific DNA sequence, referred to as Chi (chi). Recognition of chi triggers attenuation of the 3'- to 5'-nuclease, which permits the generation of recombinogenic 3'-overhanging, single-stranded DNA (ssDNA), terminating at chi. Although the RecBCD enzyme briefly pauses at chi, no specific binding of RecBCD to chi during translocation has been documented. Here, we show that the AddAB enzyme transiently binds to its cognate chi sequence (chi(Bs): 5'-AGCGG-3') during translocation. The binding of AddAB enzyme to the 3'-end of the chi(Bs)-specific ssDNA results in protection from degradation by exonuclease I. This protection is gradually reduced with time and lost upon phenol extraction, showing that the binding is non-covalent. Addition of AddAB enzyme to processed, chi(Bs)-specific ssDNA that had been stripped of all protein does not restore nuclease protection, indicating that AddAB enzyme binds to chi(Bs) with high affinity only during translocation. Finally, protection of chi(Bs)-specific ssDNA is still observed when translocation occurs in the presence of competitor chi(Bs)-carrying ssDNA, showing that binding occurs in cis. We suggest that this transient binding of AddAB to chi(Bs) is an integral part of the AddAB-chi(Bs) interaction and propose that this molecular event underlies a general mechanism for regulating the biochemical activities and biological functions of RecBCD-like enzymes.
+!align=left  |Citation
-</noinclude>
+||
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-|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16632468 PubMed]
+!align=left  |Keywords
 |}
+<!--box uid=d41d8cd98f00b204e9800998ecf8427e.113901.Y52238d1497306-->
 ==Significance {{HelpIcon|Help:Literature}} ==
 {{LitSignificance}}

127.0.0.1: /Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

2010-06-17T20:33:49Z

/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

← Older revision		Revision as of 20:33, 17 June 2010
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	{\| id="Y472eb435420f4" class=" tableEdit GO_table_reference"		{\| id="Y472eb435420f4" class=" tableEdit GO_table_reference"
	\|-		\|- {{table heading style}}
	!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

127.0.0.1: Update TableEdit tables.

2010-01-09T04:26:33Z

Update TableEdit tables.

← Older revision		Revision as of 04:26, 9 January 2010
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	\|- ~~{{table heading style}}~~		\|-
	!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

	\|-class=~~'sortbottom'~~		\|- class="tableEdit_footer"
	\|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4&page=0&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|		\|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4&page=0&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table]</span> \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
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imported>Wikientrybot: Fix links due to TableEditLinks.php changes.

2009-02-20T03:32:26Z

Fix links due to TableEditLinks.php changes.

← Older revision		Revision as of 03:32, 20 February 2009
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	<!--		<!--
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	* PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table.		* PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table.
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	\|- {{table heading style}}		\|- {{table heading style}}
	!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!\|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

	\|-class='sortbottom'		\|-class='sortbottom'
	\|[{{SERVER}}{{SCRIPTPATH}}~~/index.php~~?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4&page=0&pagename={{FULLPAGENAMEE}} edit table] \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|		\|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4&page=0&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
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127.0.0.1: Edited by wikibot

2008-04-30T12:15:39Z

Edited by wikibot

← Older revision		Revision as of 12:15, 30 April 2008
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	!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status		!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
	\|-class='sortbottom'		\|-class='sortbottom'
	\|[{{SERVER}}~~/colipedia~~/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4&page=0&pagename={{FULLPAGENAMEE}} edit table] \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|		\|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4&page=0&pagename={{FULLPAGENAMEE}} edit table] \|\| \|\| \|\| \|\| \|\| \|\| \|\| \|\|
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	<!--box uid=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4-->		<!--box uid=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4-->

127.0.0.1: Edited by wikibot

2008-04-25T22:07:24Z

Edited by wikibot

← Older revision		Revision as of 22:07, 25 April 2008
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	~~<protect>~~
	{{RightTOC}}		{{RightTOC}}

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	\|{{TableHeadingColor}} nowrap \|'''Links''' \|\| [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16632468 PubMed]		\|{{TableHeadingColor}} nowrap \|'''Links''' \|\| [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16632468 PubMed]
	\|}		\|}
	~~</protect>~~
	==Significance {{HelpIcon\|Help:Literature}} ==		==Significance {{HelpIcon\|Help:Literature}} ==
	{{LitSignificance}}		{{LitSignificance}}
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	==Annotations {{HelpIcon\|Help:Literature}}==		==Annotations {{HelpIcon\|Help:Literature}}==
	~~<protect>~~<!--box uid=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4-->		<!--box uid=63ce1649aab8b3180235b0af7618f667.0.Y472eb435420f4-->
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	==EcoliWiki Links {{HelpIcon\|Help:Literature}} ==		==EcoliWiki Links {{HelpIcon\|Help:Literature}} ==

66.249.70.172: New PMID: Page!

2007-11-05T06:12:05Z

New PMID: Page!

New page

<protect>
{{RightTOC}}

<span style = 'font-size: large'> '''Chédin, F, Handa, N, Dillingham, MS and Kowalczykowski, SC''' (2006) The AddAB helicase/nuclease forms a stable complex with its cognate chi sequence during translocation.''J. Biol. Chem.'' '''281''':18610-7</span>

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || The Bacillus subtilis AddAB enzyme possesses ATP-dependent helicase and nuclease activities, which result in the unwinding and degradation of double-stranded DNA (dsDNA) upon translocation. Similar to its functional counterpart, the Escherichia coli RecBCD enzyme, it also recognizes and responds to a specific DNA sequence, referred to as Chi (chi). Recognition of chi triggers attenuation of the 3'- to 5'-nuclease, which permits the generation of recombinogenic 3'-overhanging, single-stranded DNA (ssDNA), terminating at chi. Although the RecBCD enzyme briefly pauses at chi, no specific binding of RecBCD to chi during translocation has been documented. Here, we show that the AddAB enzyme transiently binds to its cognate chi sequence (chi(Bs): 5'-AGCGG-3') during translocation. The binding of AddAB enzyme to the 3'-end of the chi(Bs)-specific ssDNA results in protection from degradation by exonuclease I. This protection is gradually reduced with time and lost upon phenol extraction, showing that the binding is non-covalent. Addition of AddAB enzyme to processed, chi(Bs)-specific ssDNA that had been stripped of all protein does not restore nuclease protection, indicating that AddAB enzyme binds to chi(Bs) with high affinity only during translocation. Finally, protection of chi(Bs)-specific ssDNA is still observed when translocation occurs in the presence of competitor chi(Bs)-carrying ssDNA, showing that binding occurs in cis. We suggest that this transient binding of AddAB to chi(Bs) is an integral part of the AddAB-chi(Bs) interaction and propose that this molecular event underlies a general mechanism for regulating the biochemical activities and biological functions of RecBCD-like enzymes.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16632468 PubMed]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>