PMID:1663113 - Revision history

imported>Wikientrybot: change PMID template to TableEdit version

2013-09-15T04:30:10Z

change PMID template to TableEdit version

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	'''~~No author listed~~''' (1991) Amplification and substantial purification of cardiolipin synthase of Escherichia coli. ''J. Biochem.'' '''110''':443-9		'''Hiraoka, S, Nukui, K, Uetake, N, Ohta, A and Shibuya, I''' (1991) Amplification and substantial purification of cardiolipin synthase of Escherichia coli. ''J. Biochem.'' '''110''':443-9
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	!align=left \|Abstract		!align=left \|Abstract

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imported>Wikientrybot: change PMID template to TableEdit version

2013-09-01T18:53:08Z

change PMID template to TableEdit version

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	~~<span style~~ = '~~font-size: large'>~~ ''~~'Hiraoka, S, Nukui, K, Uetake, N, Ohta, A and Shibuya, I~~''' (1991) Amplification and substantial purification of cardiolipin synthase of Escherichia coli.''J. Biochem.'' '''110''':443-9</~~span>~~		\|-
			!align=left \|Citation
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			'''No author listed''' (1991) Amplification and substantial purification of cardiolipin synthase of Escherichia coli. ''J. Biochem.'' '''110''':443-9
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			!align=left \|Abstract
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			A simple, specific, and sensitive assay procedure for cardiolipin synthase of Escherichia coli has been developed. This measures the radioactivity of glycerol formed from phosphatidyl [2-3H]glycerol and is mainly based on the findings that 400 mM phosphate and 0.015% Triton X-100 markedly activate the enzyme. Cardiolipin synthase was amplified 760-fold upon induction with isopropyl beta-D-thiogalactoside in cells harboring a pBR322 derivative in which the cls gene encoding this enzyme was preceded by the tac promoter. Under these conditions, cardiolipin content increased, membrane potential decreased, spheroplasts became fragile, cells lost viability, and inducer-resistant mutants appeared at a high frequency. The amplification enabled the isolation of an enzyme preparation with a specific activity approximately 10,000-times higher than that of wild-type whole cell lysate. This purification was simply achieved by extraction of the crude membrane fraction with Triton X-100 and a single phosphocellulose column chromatography. This preparation, together with the crude envelope fraction, was used to characterize the basic properties of E. coli cardiolipin synthase, some of which were utilized in setting up the assay conditions.
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			[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1663113 PubMed]

	~~{\|{{Prettytable}} align = 'center' width= '70%'~~
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	\|~~{{TableHeadingColor}}~~ \|~~'''Abstract'''~~ \|~~\| A simple, specific, and sensitive assay procedure for cardiolipin synthase of~~ Escherichia coli has been developed. This measures the radioactivity of glycerol formed from phosphatidyl [2-3H]glycerol and is mainly based on the findings that 400 mM phosphate and 0.015% Triton X-100 markedly activate the enzyme. Cardiolipin synthase was amplified 760-fold upon induction with isopropyl beta-D-thiogalactoside in cells harboring a pBR322 derivative in which the cls gene encoding this enzyme was preceded by the tac promoter. Under these conditions, ~~cardiolipin content increased~~, ~~membrane potential decreased, spheroplasts became fragile, cells lost viability, and inducer-resistant mutants appeared at a high frequency. The amplification enabled the~~ isolation ~~of an enzyme preparation with a specific activity approximately 10,000-times higher than that of wild-type whole cell lysate. This~~ purification was simply achieved by extraction of the crude membrane fraction with Triton X-100 and a single phosphocellulose column chromatography. This preparation, together with the crude envelope fraction, was used to characterize the basic properties of E. coli cardiolipin synthase, some of which were utilized in setting up the assay conditions.		!align=left \|Keywords
	~~</noinclude>~~		\|\|
	\|-		Detergents/pharmacology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/physiology; Gene Amplification; Genes, Bacterial; Kinetics; Membrane Potentials; Membrane Proteins; Octoxynol; Phospholipids/analysis; Phospholipids/metabolism; Phosphotransferases/genetics; Phosphotransferases/isolation & purification; Phosphotransferases/metabolism; Plasmids; Polyethylene Glycols/pharmacology; Promoter Regions, Genetic; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Spheroplasts/enzymology; Spheroplasts/physiology; Transferases (Other Substituted Phosphate Groups)
	\|{{~~TableHeadingColor~~}} ~~nowrap \|'''Links''' \|\| [http~~:~~//www~~.~~ncbi~~.~~nlm.nih.gov/entrez/query.fcgi?cmd~~=~~Retrieve~~&db=~~pubmed~~&~~dopt~~=~~Abstract~~&~~list_uids~~=~~1663113 PubMed~~]
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	==Significance {{HelpIcon\|Help:Literature}} ==		==Significance {{HelpIcon\|Help:Literature}} ==
	{{LitSignificance}}		{{LitSignificance}}

127.0.0.1: /Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php

2010-06-17T20:33:48Z

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2010-01-09T04:26:33Z

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imported>Wikientrybot: Fix links due to TableEditLinks.php changes.

2009-02-20T03:32:24Z

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127.0.0.1: Edited by wikibot

2008-04-30T12:59:58Z

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127.0.0.1: Edited by wikibot

2008-04-25T22:40:01Z

Edited by wikibot

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	\|{{TableHeadingColor}} nowrap \|'''Links''' \|\| [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1663113 PubMed]		\|{{TableHeadingColor}} nowrap \|'''Links''' \|\| [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1663113 PubMed]
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	==Significance {{HelpIcon\|Help:Literature}} ==		==Significance {{HelpIcon\|Help:Literature}} ==
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	==Annotations {{HelpIcon\|Help:Literature}}==		==Annotations {{HelpIcon\|Help:Literature}}==
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	==EcoliWiki Links {{HelpIcon\|Help:Literature}} ==		==EcoliWiki Links {{HelpIcon\|Help:Literature}} ==

74.192.221.37: New PMID: Page!

2008-02-07T15:10:20Z

New PMID: Page!

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<span style = 'font-size: large'> '''Hiraoka, S, Nukui, K, Uetake, N, Ohta, A and Shibuya, I''' (1991) Amplification and substantial purification of cardiolipin synthase of Escherichia coli.''J. Biochem.'' '''110''':443-9</span>

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || A simple, specific, and sensitive assay procedure for cardiolipin synthase of Escherichia coli has been developed. This measures the radioactivity of glycerol formed from phosphatidyl [2-3H]glycerol and is mainly based on the findings that 400 mM phosphate and 0.015% Triton X-100 markedly activate the enzyme. Cardiolipin synthase was amplified 760-fold upon induction with isopropyl beta-D-thiogalactoside in cells harboring a pBR322 derivative in which the cls gene encoding this enzyme was preceded by the tac promoter. Under these conditions, cardiolipin content increased, membrane potential decreased, spheroplasts became fragile, cells lost viability, and inducer-resistant mutants appeared at a high frequency. The amplification enabled the isolation of an enzyme preparation with a specific activity approximately 10,000-times higher than that of wild-type whole cell lysate. This purification was simply achieved by extraction of the crude membrane fraction with Triton X-100 and a single phosphocellulose column chromatography. This preparation, together with the crude envelope fraction, was used to characterize the basic properties of E. coli cardiolipin synthase, some of which were utilized in setting up the assay conditions.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1663113 PubMed]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>