DB3.1 - Revision history

Wouter.deschamphelaire at 11:21, 28 October 2024

2024-10-28T11:21:07Z

← Older revision		Revision as of 11:21, 28 October 2024
Line 14:		Line 14:
	\|-		\|-
	\| Sources		\| Sources
	\| [https://bccm.belspo.be/~~catalogues~~/genecorner-~~host~~-~~details?NUM=LMBP%204098~~ LMBP 4098]		\| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/391 LMBP 4098]
	\|-		\|-
	\| References		\| References

Wouter.deschamphelaire at 11:21, 16 October 2020

2020-10-16T11:21:47Z

← Older revision		Revision as of 11:21, 16 October 2020
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	\|-		\|-
	\| Sources		\| Sources
	\| [~~http~~://bccm.belspo.be/catalogues~~/files~~/genecorner-~~hosts/DB3~~-~~1.pdf~~ LMBP 4098]		\| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%204098 LMBP 4098]
	\|-		\|-
	\| References		\| References

Wouter.deschamphelaire at 13:29, 16 August 2018

2018-08-16T13:29:02Z

← Older revision		Revision as of 13:29, 16 August 2018
Line 14:		Line 14:
	\|-		\|-
	\| Sources		\| Sources
	\| [http://bccm.belspo.be/catalogues/files/~~lmbp~~-hosts/~~db3~~-1.pdf LMBP 4098]		\| [http://bccm.belspo.be/catalogues/files/genecorner-hosts/DB3-1.pdf LMBP 4098]
	\|-		\|-
	\| References		\| References
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	Alternative: ccdB-resistant One Shot® ccdB Survival™ 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent.		Alternative: ccdB-resistant One Shot® ccdB Survival™ 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent.

	[[Category:BCCM/~~LMBP~~]][[Category:Strains]]		[[Category:BCCM/GeneCorner]][[Category:Strains]]

Wouter.deschamphelaire at 08:55, 4 July 2016

2016-07-04T08:55:48Z

← Older revision		Revision as of 08:55, 4 July 2016
Line 12:		Line 12:
	\| Phenotype		\| Phenotype
	\| Streptomycin resistant, ccdB resistant		\| Streptomycin resistant, ccdB resistant
	\|-
	~~\| Plasmids~~
	~~\| DB3.1~~
	\|-
	~~\| Phage~~
	~~\| DB3.1~~
	\|-		\|-
	\| Sources		\| Sources
	\| [http://bccm.belspo.be/catalogues/files/lmbp-hosts/db3-1.pdf LMBP 4098]		\| [http://bccm.belspo.be/catalogues/files/lmbp-hosts/db3-1.pdf LMBP 4098]
	\|-
	~~\| Construction~~
	~~\| DB3.1~~
	\|-		\|-
	\| References		\| References

Wouter.deschamphelaire: /* Notes */

2016-07-04T08:46:17Z

Notes

← Older revision		Revision as of 08:46, 4 July 2016
Line 35:		Line 35:
	Alternative: ccdB-resistant One Shot® ccdB Survival™ 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent.		Alternative: ccdB-resistant One Shot® ccdB Survival™ 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent.

	[[Category:Strains]]		[[Category:BCCM/LMBP]][[Category:Strains]]

Wouter.deschamphelaire: /* Notes */

2016-07-04T08:35:37Z

Notes

← Older revision		Revision as of 08:35, 4 July 2016
Line 34:		Line 34:
	Original Invitrogen catalogue number: 11782-018. However, this strain is no longer available at Invitrogen. <br />		Original Invitrogen catalogue number: 11782-018. However, this strain is no longer available at Invitrogen. <br />
	Alternative: ccdB-resistant One Shot® ccdB Survival™ 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent.		Alternative: ccdB-resistant One Shot® ccdB Survival™ 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent.

			[[Category:Strains]]

Wouter.deschamphelaire: Created page with "{| class="wikitable" border="1" |- | Name | DB3.1 |- | Synonyms | |- | Genotype | gyrA462 endA1 ∆(sr1-recA) mcrB mrr hsdS20 glnV44 (=supE44) ara14 galK2 lacY1 proA2 rpsL20 x..."

2016-07-04T07:42:15Z

Created page with "{| class="wikitable" border="1" |- | Name | DB3.1 |- | Synonyms | |- | Genotype | gyrA462 endA1 ∆(sr1-recA) mcrB mrr hsdS20 glnV44 (=supE44) ara14 galK2 lacY1 proA2 rpsL20 x..."

New page

{| class="wikitable" border="1"
|-
| Name
| DB3.1
|-
| Synonyms
|
|-
| Genotype
| gyrA462 endA1 ∆(sr1-recA) mcrB mrr hsdS20 glnV44 (=supE44) ara14 galK2 lacY1 proA2 rpsL20 xyl5 leuB6 mtl1
|-
| Phenotype
| Streptomycin resistant, ccdB resistant
|-
| Plasmids
| DB3.1
|-
| Phage
| DB3.1
|-
| Sources
| [http://bccm.belspo.be/catalogues/files/lmbp-hosts/db3-1.pdf LMBP 4098]
|-
| Construction
| DB3.1
|-
| References
| PMID:1324324
|}

===Notes===
DB3.1 is a HB101 derivative containing the gyrA462 allele which renders the strain resistant to the toxic effects of the ccdB gene. <br />
<br />
Original Invitrogen catalogue number: 11782-018. However, this strain is no longer available at Invitrogen. <br />
Alternative: ccdB-resistant One Shot® ccdB Survival™ 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent.