EcoliWiki - User contributions [en]

Category:Strain:Hfr 3000

2024-10-28T11:34:20Z

Wouter.deschamphelaire:

{{See|Help:Strains|strains}}
<protect>

{| id="W4e1741c80cbcc" class=" tableEdit Strain_Geneology_Table dataTable"

|-
!align=left {{table_heading_style}} |Name
||
Hfr3000
|-
!align=left {{table_heading_style}} |Synonyms
||
HfrH Thi- λ-; HfrC (Paris), AB259, K12 3000
|-
!align=left {{table_heading_style}} |Genotype
||
Hfr (PO1)''relA1 spoT1 thi-1''
|-
!align=left {{table_heading_style}} |Phenotype
||

|-
!align=left {{table_heading_style}} |Plasmids
||
Hfr
|-
!align=left {{table_heading_style}} |Phage
||
λ-
|-
!align=left {{table_heading_style}} |Sources
||
[http://cgsc.biology.yale.edu/Strain.php?ID=4637 CGSC:259] 
[https://bccm.belspo.be/page/genecorner-hosts-display-razor/103 LMBP 1393]
|-
!align=left {{table_heading_style}} |Construction
||
Strain Hfr 3000 is a UV treated isolate of strain HfrH Thi- 
|-
!align=left {{table_heading_style}} |References
||
PMID:5340246 PMID:4568763, PMID:4148765

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.178252.W4e1741c80cbcc&page=178252&pagename={{FULLPAGENAMEE}}&type=1&template=Strain_Geneology_Table edit table]</div> ||
|}
</protect>

== Notes ==
The strain is useful for propagation of F+ specific phages.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

==References==
{{RefHelp}}
<references/>

__NOTOC__

WK6mutS(λ)

2024-10-28T11:33:06Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| WK6mutS(λ)
|-
| Synonyms
|
|-
| Genotype
| F' lacIq ∆(lacZ)M15 proA+B+ traD36I ∆(lac-proB) galE rpsL mutS::Tn10 λ
|-
| Phenotype
| Streptomycin resistant, tetracyclin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/388 LMBP 1466]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=3038536 PMID:3038536] [http://www.ncbi.nlm.nih.gov/pubmed/?term=2501754 PMID:2501754]
|}

===Notes===
Useful strain for site-directed mutagenesis; disabled mismatch repair due to knocked-out mutS. 
The Tn10 insertion renders the strain tetR. 
Not to be used as permanent storage host for plasmids because of the danger of accumulating spontaneous mutations. 

[[Category:BCCM/GeneCorner]][[Category:Strains]]

SM10(λpir)

2024-10-28T11:32:31Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| SM10(λpir)
|-
| Synonyms
| SM10(lambda pir)
|-
| Genotype
| thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km λpir
|-
| Phenotype
| Kanamycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/359 LMBP 3889]
|-
| References
| Simon R, Priefer U, Puhler A (1983) A broad host range mobilization system for invivo genetic-engineering - transposon mutagenesis in gram-negative bacteria. Bio-Technol 1: 784–791
|}

===Notes===
Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome. 
Useful for mobilizing mobRP4 plasmids. 
The presence of λpir allows the replication of a defective R6K suicide plasmid and derivatives thereof.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

SG4121

2024-10-28T11:32:09Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| SG4121
|-
| Synonyms
|
|-
| Genotype
| lon-100 Δ(gal-pgl)324 lac thi rpsL recA hsdR
|-
| Phenotype
| Streptomycin resistant, non-mucoid, Lon-
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/351 LMBP 671]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=1312934 PMID:1312934]
|}

===Notes===
The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology. 
Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli (see e.g. Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688). 
In addition, this strain is K12 restriction negative / modification positive, and RecA deficient.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

SG4044

2024-10-28T11:31:53Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| SG4044
|-
| Synonyms
|
|-
| Genotype
| lon-100 Δ(gal-pgl)324 lac thi rpsL
|-
| Phenotype
| Streptomycin resistant, non-mucoid, Lon-
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/348 LMBP 670]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=7026534 PMID:7026534] [http://www.ncbi.nlm.nih.gov/pubmed/?term=3888955 PMID:3888955]
|}

===Notes===
The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology. 
Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli (see e.g. Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688).

[[Category:BCCM/GeneCorner]][[Category:Strains]]

MG1655 seqA-PAmCherry

2024-10-28T11:31:20Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| MG1655 seqA-PAmCherry
|-
| Synonyms
|
|-
| Genotype
| F– λ– ilvG– rfb-50 rph-1 seqA-PAmCherry:kmR
|-
| Phenotype
| Kanamycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/662 LMBP 9778]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=26449690 PMID:26449690]
|}

===Notes===
The native seqA gene was fused at its 3' end to the fluorescent protein gene PAmCherry, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome. 
PAmCherry is a photoactivatable variant of the red fluorescent protein mCherry. PAmCherry is not fluorescent until it is exposed to 350-400 nm light. 
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

MG1655 seqA-mEOS3.2

2024-10-28T11:31:04Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| MG1655 seqA-mEOS3.2
|-
| Synonyms
|
|-
| Genotype
| F– λ– ilvG– rfb-50 rph-1 seqA-mEOS3.2:kmR
|-
| Phenotype
| Kanamycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/661 LMBP 9777]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=26449690 PMID:26449690]
|}

===Notes===
The native seqA gene was fused at its 3' end to the fluorescent protein gene mEOS3.2, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome. 
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

MG1655 seqA-eYFP

2024-10-28T11:30:45Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| MG1655 seqA-eYFP
|-
| Synonyms
|
|-
| Genotype
| F– λ– ilvG– rfb-50 rph-1 seqA-eYFP:kmR
|-
| Phenotype
| Kanamycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/660 LMBP 9776]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=26449690 PMID:26449690]
|}

===Notes===
The native seqA gene was fused at its 3' end to the fluorescent protein gene eYFP, separated with a linker (ASPPPGRSR) and followed by a kanamycin resistance marker. The entire cassette was integrated in the chromosome. 
This strain can be used to study the subcellular localization of the E. coli DNA binding protein SeqA.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

MC1061λ

2024-10-28T11:29:52Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| MC1061λ
|-
| Synonyms
| MC1061lambda
|-
| Genotype
| ∆(araA-leu)7697 [araD139]B/r ∆(codB-lacI)3 = ∆lac74 galK16 galE15 e14- mcrA0 relA1 rpsL150 spoT1 mcrB1 hsdR2 λ+
|-
| Phenotype
| Streptomycin resistant, zeocin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/277 LMBP 1061]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=7590329 PMID:7590329] [http://www.ncbi.nlm.nih.gov/pubmed/?term=6997493 PMID:6997493]
|}

===Notes===
Useful host for primary transformation. Transforms very well by the CaCl2 method (107/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification. 
Lysogenized with λ wild type. 
Recommended as maintenance host for plasmids containing the PL or PR promoter from phage λ. 
It is recommended to grow MC1061(λ), containing pPL plasmids, at 28°C. 
As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as ''tac'', ''trc'', ''N25/O2'' … promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability. 
The zeocin resistance is most probably related to the presence of a functional recA gene (recA+).

[[Category:BCCM/GeneCorner]][[Category:Strains]]

MC1061Rif

2024-10-28T11:29:37Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| MC1061Rif
|-
| Synonyms
|
|-
| Genotype
| ∆(araA-leu)7697 [araD139]B/r ∆(codB-lacI)3 = ∆lac74 galK16 galE15 e14- mcrA0 relA1 rpsL150 spoT1 mcrB1 hsdR2 λ+
|-
| Phenotype
| rifampicin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/276 LMBP 3319]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/?term=6997493 PMID:6997493]
|}

===Notes===
Useful host for primary transformation. Transforms very well by the CaCl2 method (107/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification. 
Lysogenized with λ wild type. 
As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as ''tac'', ''trc'', ''N25/O2'' … promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability. 

[[Category:BCCM/GeneCorner]][[Category:Strains]]

M5219

2024-10-28T11:29:15Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| KM5219
|-
| Synonyms
|
|-
| Genotype
| lacZam trpAam rpsL lambda(bio252 cI857 ΔH1)
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/253 LMBP 130]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/6271633 PMID:6271633]
|}

===Notes===
Expression host for plasmids containing phage lambda's PL or PR promoter. Induction is obtained by shifting the culture from 28°C to 42°C. The resident lambda is a defective lysogen. ΔH1 removes part of cro and all genes to the right of it. bio252 removes all genes to the left of cIII. cI857 is ind-. At 42°C, N is expressed from the lysogen. As a consequence, transcription termination signals that might be present downstream from the PL promoter and the nutL site, as present on the pPL-series of plasmids, can be overridden.
Useful for the expression of cloned genes carrying transcription terminators between the start of the gene and the insertion point.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

LK111

2024-10-28T11:27:25Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| LK111
|-
| Synonyms
|
|-
| Genotype
| F' lac-pro Δ(lacZ)M15 / thi-1 thr-1 leuB6 tonA lacI- Δ(lacZ)M15 lacY+ supE44 P1s
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/242 LMBP 4706]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/6327257 PMID:6327257]
|}

===Notes===
This strain is a derivative of E. coli K12 K514, which is a rK- mK+ derivative of E. coli K12 C600.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

K12ΔH1Δtrp

2024-10-28T11:27:06Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| K12ΔH1Δtrp
|-
| Synonyms
| K12deltaH1deltatrp
|-
| Genotype
| lacZam Δ(bio-uvrB) Δ(trpE-A)2 rpsL Δ(Nam7Nam53 cI857 ΔH1)
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/403 LMBP 69]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/372049 PMID:372049] [http://www.ncbi.nlm.nih.gov/pubmed/6271633 PMID:6271633]
|}

===Notes===
Expression host for plasmids containing phage lambda's PL or PR promoter. Induction is obtained by shifting the culture from 28°C to 42°C. The resident lambda is a defective lysogen. ΔH1 removes part of cro and all genes to the right of it. cI857 is ind-.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

JK242

2024-10-28T11:26:45Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| JK242
|-
| Synonyms
|
|-
| Genotype
| asnA31 asnB32 thi-1 relA spoT rpsL125
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/452 LMBP 917]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/6780757 PMID:6780757]
|}

===Notes===
The JK242 strain is auxotrophic for asparagine because of the inactivated asnA and asnB gene. It carries also the streptomycin-resistant allele, rpsL, a mutation leading to increased translational accuracy.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

IM93B

2024-10-28T11:26:27Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| IM93B
|-
| Synonyms
|
|-
| Genotype
| mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG Δdcm ΩPN25-hsdMS/S (CC93-2/CC93-1) ΩPhelp-hsdMS (CC93-3)
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/654 LMBP 9584]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/26015493 PMID:26015493]
|}

===Notes===
This bacterial host strain was derived from E. coli K12 DH10B by deleting the dcm gene encoding cytosine methylation. Additionally, the hsdMS genes encoding methylase and specificity genes (from Staphylococcus aureus sequence type 93 - JKD6159) were introduced onto the chromosome at neutral locations via recombineering. 
The strain can be transformed efficiently with large plasmids due to deoR (nupG) mutation. 
Plasmids isolated from this strain transform S. aureus sequence type 93 - JKD6159 at high efficiency.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

IM30B

2024-10-28T11:26:09Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| IM30B
|-
| Synonyms
|
|-
| Genotype
| mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG Δdcm ΩPhelp-hsdMS (CC30-2) ΩPN25-hsdS (CC30-1)
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/653 LMBP 9583]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/26015493 PMID:26015493]
|}

===Notes===
This bacterial host strain was derived from E. coli K12 DH10B by deleting the dcm gene encoding cytosine methylation. Additionally, the hsdMS genes encoding methylase and specificity genes (from Staphylococcus aureus clonal complex 30 - MRSA252) were introduced onto the chromosome at neutral locations via recombineering. 
The strain can be transformed efficiently with large plasmids due to deoR (nupG) mutation. 
Plasmids isolated from this strain transform S. aureus clonal complex 30 - MRSA252 at high efficiency.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

IM08B

2024-10-28T11:25:52Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| IM08B
|-
| Synonyms
|
|-
| Genotype
| mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG Δdcm ΩPhelp-hsdMS (CC8-2) ΩPN25-hsdS (CC8-1)
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/652 LMBP 9582]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/26015493 PMID:26015493]
|}

===Notes===
This bacterial host strain was derived from E. coli K12 DH10B by deleting the dcm gene encoding cytosine methylation. Additionally, the hsdMS genes encoding methylase and specificity genes (from Staphylococcus aureus clonal complex 8 – NRS384) were introduced onto the chromosome at neutral locations via recombineering. 
The strain can be transformed efficiently with large plasmids due to deoR (nupG) mutation. 
Plasmids isolated from this E. coli strain transform S. aureus clonal complex 8 – NRS384 at high efficiency.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

IM01B

2024-10-28T11:25:36Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| IM01B
|-
| Synonyms
|
|-
| Genotype
| mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG Δdcm ΩPhelp-hsdMS (CC1-2) ΩPN25-hsdS (CC1-1)
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/651 LMBP 9581]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/26015493 PMID:26015493]
|}

===Notes===
This bacterial host strain was derived from E. coli K12 DH10B by deleting the dcm gene encoding cytosine methylation. Additionally, the hsdMS genes encoding methylase and specificity genes (from Staphylococcus aureus clonal complex 1 - MW2) were introduced into the chromosome at neutral locations via recombineering. 
The strain can be transformed efficiently with large plasmids due to deoR (nupG) mutation. 
Plasmids isolated from this E. coli strain transform S. aureus clonal complex 1 - MW2 at high efficiency.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

HB94

2024-10-28T11:24:57Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| HB94
|-
| Synonyms
|
|-
| Genotype
| F- thr-1 araC14 leuB6(Am) fhuA31 lacY1 tsx-78 glnX44(AS) galK2(Oc) galT22 λ- dcm-6 hisG4(Oc) rpsL136 xylA5 mtl-1 thiE1
|-
| Phenotype
|
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/200 LMBP 7992]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/4896022 PMID:4896022]
|}

[[Category:BCCM/GeneCorner]][[Category:Strains]]

HB101

2024-10-28T11:24:37Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| HB101
|-
| Synonyms
|
|-
| Genotype
| Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center.
 F- Lambda- araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(rB-, mB-) 
NEB: includes DE(mcrC-mrr) 
Promega (and others): proA2
|-
| Phenotype
| Streptomycin resistance 
Lacks the K-12 restriction-modification system
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/392 LMBP 3040] 
[http://cgsc.biology.yale.edu/Strain.php?ID=130465 CGSC 12554]
|-
| References
| Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459. Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56. Lacks S and Greenberg JR (1977) J Mol Biol 114:153.
|}

===Notes===

[[Category:BCCM/GeneCorner]][[Category:Strains]]

GM31

2024-10-28T11:24:06Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| GM31
|-
| Synonyms
|
|-
| Genotype
| F- thr-1 araC14 leuB6(Am) fhuA31 lacY1 tsx-78 glnX44(AS) galK2(Oc) galT22 λ- dcm-6 hisG4(Oc) rpsL136 xylA5 mtl-1 thiE1
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/194 LMBP 4433]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/4589344 PMID:4589344]
|}

===Notes===
This is a dcm- strain.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

GM2198

2024-10-28T11:23:52Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| GM2198
|-
| Synonyms
|
|-
| Genotype
| F- thr-1 araC14 leuB6(Am) fhuA31 lacY1 tsx-78 glnX44(AS) galK2(Oc) λ- dcm-6 hisG4(Oc) rpsL136 dam-13::Tn9 xylA5 mtl-1 thiE1
|-
| Phenotype
| streptomycin and chloramphenicol resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/193 LMBP 4435]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/6316110 PMID:6316110]
|}

===Notes===
This is a dam- and dcm- strain.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

GM124

2024-10-28T11:23:37Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| GM124
|-
| Synonyms
|
|-
| Genotype
| F- lacZ118(Oc) rpsL275 dam-4
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/191 LMBP 4434]
|-
| References
|
|}

===Notes===
This is a dam- strain.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

FHK12

2024-10-28T11:23:16Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| FHK12
|-
| Synonyms
|
|-
| Genotype
| F' lacIq lacZΔM15 proA+B+ ara Δ(lac-proAB) rpsL (ϕ80dlacZΔM15) attB::(ctx::lacZ) (source: Kolmar et al., 1995)
|-
| Phenotype
| ampicillin and streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/684 LMBP 11202]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/7664730 PMID:7664730]
|}

===Notes===
The ctx::lacZ fusion integrated at the attB site is tagged with the ampicillin resistance gene. This FHK12 strain was constructed as follows: 
First FHK11 was constructed from strain JM83 (Yanish-Perron et al., 1985) by chromosomal integration of the lacZ gene under control of the V. cholerae ctxAB promoter from plasmid pLDR10-ctx::lacZ at the phage λ attB site (Diederich et al., 1992).

[[Category:BCCM/GeneCorner]][[Category:Strains]]

DP50

2024-10-28T11:22:50Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| DP50
|-
| Synonyms
|
|-
| Genotype
| F-, fhuA53, dapD8, lacY1 or Δ(cod-lacI)6, glnX44(AS), Δ(gal-uvrB)47, λ-, gyrA29, ΔthyA57, hsdS3
|-
| Phenotype
| nalidixic acid resistant
|-
| Sources
| [https://bccm.belspo.be/genecorner-host-search (strain was removed from collection)]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/322278 PMID:322278]
|}

===Notes===
This strain is used for propagation of phage lambda and phage lambda Charon.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

DH5alpha F(λ)

2024-10-28T11:22:19Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| DH5alpha F(λ)
|-
| Synonyms
| DH5αF(λ)
|-
| Genotype
| F+ Δ(argF-lac)169 φ80dlacZ58(M15) ΔphoA8 glnX44(AS) λ+ deoR481 rfbC1 gyrA96 recA1 endA1 thiE1 hsdR17
|-
| Phenotype
| nalidixic acid resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/165 LMBP 2846]
|-
| References
|
|}

===Notes===
Useful strain for Lacα complementation. This strain does not contain the lacIq gene and therefore repression on the lac promoter, present on high-copy plasmids, is incomplete. 
This strain contains the F factor of NK3 and therefore can be used for infection with M13 or fd phages and derivatives. In addition the strain is lysogenic for wild-type phage λ.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

DH5 alpha pir116 variant

2024-10-28T11:22:03Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| DH5αpir116 variant
|-
| Synonyms
| DH5 alpha pir116 variant
|-
| Genotype
| endA1 hsdR17 glnV44 (= supE44) thi-1 recA1 gyrA96 relA1 φ80dlacΔ(lacZ)M15 Δ(lacZYA-argF)U169 zdg-232::Tn10 uidA::pir+
|-
| Phenotype
| tetracyclin sensitive
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/168 LMBP 7961]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/2162051 PMID:2162051] [http://www.ncbi.nlm.nih.gov/pubmed/10610816 PMID:10610816] [http://www.ncbi.nlm.nih.gov/pubmed/21376749 PMID:21376749]
|}

===Notes===
The E. coli K12 DH5αpir116 variant appears to have lost the Tn10 and thus is tetracycline sensitive .

[[Category:BCCM/GeneCorner]][[Category:Strains]]

DH5 alpha pir

2024-10-28T11:21:48Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| DH5αpir
|-
| Synonyms
| DH5 alpha pir
|-
| Genotype
| endA1 hsdR17 glnV44 (= supE44) thi-1 recA1 gyrA96 relA1 φ80dlacΔ(lacZ)M15 Δ(lacZYA-argF)U169 zdg-232::Tn10 uidA::pir+
|-
| Phenotype
| tetracyclin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/167 LMBP 7962]
|-
| References
| [http://www.ncbi.nlm.nih.gov/pubmed/10610816 PMID:10610816]
|}

[[Category:BCCM/GeneCorner]][[Category:Strains]]

DC10B

2024-10-28T11:21:26Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| DC10B
|-
| Synonyms
|
|-
| Genotype
| mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG Δdcm
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/655 LMBP 9585]
|-
| References
| PMID:22434850
|}

===Notes===
This bacterial host strain is a DNA cytosine methyltransferase negative mutant of E. coli K12 [[DH10B]]. 
It is a universal Staphylococcal cloning host.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

DB3.1

2024-10-28T11:21:07Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| DB3.1
|-
| Synonyms
|
|-
| Genotype
| gyrA462 endA1 ∆(sr1-recA) mcrB mrr hsdS20 glnV44 (=supE44) ara14 galK2 lacY1 proA2 rpsL20 xyl5 leuB6 mtl1
|-
| Phenotype
| Streptomycin resistant, ccdB resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/391 LMBP 4098]
|-
| References
| PMID:1324324
|}

===Notes===
DB3.1 is a HB101 derivative containing the gyrA462 allele which renders the strain resistant to the toxic effects of the ccdB gene. 
 
Original Invitrogen catalogue number: 11782-018. However, this strain is no longer available at Invitrogen. 
Alternative: ccdB-resistant One Shot® ccdB Survival™ 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

BW313

2024-10-28T11:20:28Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| BW313
|-
| Synonyms
|
|-
| Genotype
| Hfr ung-1 relA1 dut-1 spoT1 thi-1
|-
| Phenotype
|
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/125 LMBP 1325]
|-
| References
| PMID:3881765 PMID:7047496
|}

===Notes===
Use of this strain is required for the preparation of single-stranded phage M13 or phasmid DNA to be used in the site-directed mutagenesis method of Kunkel [PMID: 3881765].
[[Category:BCCM/GeneCorner]][[Category:Strains]]

BL21pT7POL26

2024-10-28T11:19:57Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| BL21pT7POL26
|-
| Synonyms
| BL21[pT7POL26]
|-
| Genotype
| F- ompT gal dcm lon hsdSB(rB-mB-) [malB+]K-12(λS)
|-
| Phenotype
| kanamycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/649 LMBP 9558]
|-
| References
|
|}

===Notes===
This strain contains the low-copy auxiliary pT7POL26 vector for T7 promoter-driven protein expression, which is repressed until IPTG induction of T7 RNA polymerase expression occurs. The strain does not contain the lon protease and is also deficient in the outer membrane protease OmpT. The lack of two key proteases reduces the degradation of heterologous proteins expressed in the strain.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

BL21(DE3)pLysS

2024-10-28T11:18:39Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| BL21(DE3)pLysS
|-
| Synonyms
|
|-
| Genotype
| F(-) ompT gal dcm lon hsdSB(rB(-) mB(-)) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
|-
| Phenotype
| chloramphenicol resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/78 LMBP 2462]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/2023259 PMID:2023259]
|}

==Plasmids ==
[[pLysS]]

==Phage ==
[[λDE3]]

==Phenotypes ==
Cmr

==Construction ==

==Notes==
The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase.
Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs.
The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteins expressed in the strain.
The helper plasmid pLysS constitutively expresses low levels of T7 lysozyme, which reduces basal level expression of recombinant genes by inhibiting basal levels of T7 RNA polymerase.
pLysS also carries the chloramphenicol resistance gene and the p15A origin for compatibility with plasmids containing the ColE1 or pMB1 origin.

==References==
Studier and Moffatt, J. Mol. Biol. 189 (1986), 113-130 [https://www.ncbi.nlm.nih.gov/pubmed/3537305 PMID: 3537305]

__NOTOC__
[[Category:Strains]]
[[Category: Expression Strains]]
[[Category: Stratagene Strains]]
[[Category: Promega Strains]]
[[Category:BCCM/GeneCorner]]

BL21(DE3)pLysE

2024-10-28T11:16:15Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| BL21(DE3)pLysE
|-
| Synonyms
|
|-
| Genotype
| F(-) ompT gal dcm lon hsdSB(rB(-) mB(-)) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
|-
| Phenotype
| chloramphenicol resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/77 LMBP 2468]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/2023259 PMID:2023259]
|}

===Notes===
The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteins expressed in the strain. The helper plasmid pLysE produces higher amounts of T7 lysozyme than pLysS to further reduce basal level expression of recombinant genes by inhibiting basal levels of T7 RNA polymerase. This makes BL21(DE3)[pLysE] more suitable for expressing toxic genes. pLysE also carries the chloramphenicol resistance gene and the p15A origin for compatibility with plasmids containing the ColE1 or pMB1 origin. 
The full genome sequence of the BL21(DE3) strain is available at the ENA/GenBank nucleotide sequence databases under accession number CP001509.3.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

BL21(DE3)

2024-10-28T11:13:06Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| BL21(DE3)
|-
| Synonyms
|
|-
| Genotype
| ''E. coli B dcm ompT hsdS''(rB-mB-) ''gal''
|-
| Phenotype
|-
| Phage
| [[lambda DE3|λDE3]]
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/75 LMBP 1455]
|-
| References
| [https://www.ncbi.nlm.nih.gov/pubmed/3537305 PMID: 3537305]
|}

===Notes===

The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs.
The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteinsexpressed in the strain.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

BHB2688

2024-10-28T11:12:38Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| BHB2688
|-
| Synonyms
|
|-
| Genotype
| F- λ[Eam4 b2 red3 imm434 cIts Sam7] recA3 IN(rrnD-rrnE)1 rpsL200
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/121 LMBP 8016] [https://www.lgcstandards-atcc.org/products/all/35131.aspx ATCC 35131] [https://www.dsmz.de/collection/catalogue/details/culture/DSM-5451 DSM 5451] [https://cgsc.biology.yale.edu/StrainQueryForm.php CGSC 13584]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/161604 PMID:161604]
|}

===Notes===
The strain is meant for in vitro packaging of bacteriophage lambda and cosmid DNA and is used for the preparation of freeze-thaw lysates (FTL).

[[Category:BCCM/GeneCorner]][[Category:Strains]]

3000

2024-10-28T11:11:50Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| 3000
|-
| Synonyms
|
|-
| Genotype
| HfrH relA1 spoT1 thi-1
|-
| Phenotype
|
|-
| Sources
| [https://bccm.belspo.be/page/genecorner-hosts-display-razor/103 LMBP 1393]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/5340246 PMID:5340246]
|}

===Notes===
Useful for propagation of F' specific phages.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

Category:Strain:S26 R1e

2024-10-28T11:10:21Z

Wouter.deschamphelaire: Table edited by $2 via TableEdit

{{See|Help:Strains|strains}}


{| id="X4e566ed5ab50d" class=" tableEdit Strain_Geneology_Table dataTable"

|-
!align=left {{table_heading_style}} |Name
||
S26 R1e
|-
!align=left {{table_heading_style}} |Synonyms
||
K37
|-
!align=left {{table_heading_style}} |Genotype
||
Hfr (PO2A) ''garB10 fhuA22 phoA4(Am) ompF627(T2R) serU132(AS) fadL701(T2R) relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510''
|-
!align=left {{table_heading_style}} |Phenotype
||
phosphatase positive
|-
!align=left {{table_heading_style}} |Plasmids
||
Hfr
|-
!align=left {{table_heading_style}} |Phage
||
λ+
|-
!align=left {{table_heading_style}} |Sources
||
[http://cgsc.biology.yale.edu/Strain.php?ID=11298 CGSC:2598] 
[https://bccm.belspo.be/page/genecorner-hosts-display-razor/343 LMBP 4978]
|-
!align=left {{table_heading_style}} |Construction
||
Strain S26 R1e is a spontaneous isolate of strain S26
|-
!align=left {{table_heading_style}} |References
||
PMID:5327650 PMID:4564480 PMID:14337506

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.180019.X4e566ed5ab50d&page=180019&pagename={{FULLPAGENAMEE}}&type=1&template=Strain_Geneology_Table edit table]</div> ||
|}


== Notes ==
This strain carries the S26 amber mutation (TAG) in the phosphatase gene. 
 
This strain carries the supD suppressor (inserts serine at the amber position) and is therefore capable of suppressing the S26 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.

==References==
{{RefHelp}}
<references/>

__NOTOC__
[[Category:Strains]][[Category:BCCM/GeneCorner]]

Category:Strain:S26 R1d

2024-10-28T11:09:58Z

Wouter.deschamphelaire: Table edited by $2 via TableEdit

{{See|Help:Strains|strains}}


{| id="B4e567086e9260" class=" tableEdit Strain_Geneology_Table dataTable"

|-
!align=left {{table_heading_style}} |Name
||
S26R1d
|-
!align=left {{table_heading_style}} |Synonyms
||

|-
!align=left {{table_heading_style}} |Genotype
||
Hfr (PO2A) ''fhuA22 phoA4(Am) glnV33(AS) ompF627(T2R) fadL701(T2R) relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510''
|-
!align=left {{table_heading_style}} |Phenotype
||
phosphatase positive
|-
!align=left {{table_heading_style}} |Plasmids
||
Hfr
|-
!align=left {{table_heading_style}} |Phage
||
λ+
|-
!align=left {{table_heading_style}} |Sources
||
[http://cgsc.biology.yale.edu/Strain.php?ID=11603 CGSC:2617] 
[https://bccm.belspo.be/page/genecorner-hosts-display-razor/342 LMBP 4979]
|-
!align=left {{table_heading_style}} |Construction
||
Strain S26R1d is a spontaneous isolate of strain S26
|-
!align=left {{table_heading_style}} |References
||
PMID:13896933 PMID:5327650 PMID:4568763 PMID:4564480 PMID:4286570

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.180021.B4e567086e9260&page=180021&pagename={{FULLPAGENAMEE}}&type=1&template=Strain_Geneology_Table edit table]</div> ||
|}


== Notes ==
This strain carries the S26 amber mutation (TAG) in the phosphatase gene. 
 
This strain carries the supE suppressor (inserts glutamine at the amber position) and is therefore capable of suppressing the S26 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.

==References==
{{RefHelp}}
<references/>

__NOTOC__
[[Category:Strains]][[Category:BCCM/GeneCorner]]

Category:Strain:S26

2024-10-28T11:09:27Z

Wouter.deschamphelaire: Table edited by $2 via TableEdit

{{See|Help:Strains|strains}}


{| id="K4e554d66921bc" class=" tableEdit Strain_Geneology_Table dataTable"

|-
!align=left {{table_heading_style}} |Name
||
S26
|-
!align=left {{table_heading_style}} |Synonyms
||
K38
|-
!align=left {{table_heading_style}} |Genotype
||
Hfr(PO2A) ''fhuA22 garB10 ompF627 fadL701 relA1 pit10 spoT1 metB1 mcrB1 cre-510 phoA4''
|-
!align=left {{table_heading_style}} |Phenotype
||
phosphatase negative
|-
!align=left {{table_heading_style}} |Plasmids
||
Hfr
|-
!align=left {{table_heading_style}} |Phage
||
λ-
|-
!align=left {{table_heading_style}} |Sources
||
[http://cgsc.biology.yale.edu/Strain.php?ID=11607 CGSC:4620] 
[https://bccm.belspo.be/page/genecorner-hosts-display-razor/341 LMBP 4977]
|-
!align=left {{table_heading_style}} |Construction
||
Strain S26 is a ethyl methanesulfonate treated isolate of strain K10
|-
!align=left {{table_heading_style}} |References
||
PMID:13896933
PMID:5327650
PMID:4917369
PMID:4568763
PMID:4564480

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.179976.K4e554d66921bc&page=179976&pagename={{FULLPAGENAMEE}}&type=1&template=Strain_Geneology_Table edit table]</div> ||
|}


== Notes ==
This strain carries the S26 amber mutation (TAG) in the phosphatase gene.

==References==
{{RefHelp}}
<references/>

__NOTOC__
[[Category:Strains]][[Category:BCCM/GeneCorner]]

Category:Strain:MC1061

2024-10-28T11:08:32Z

Wouter.deschamphelaire: Table edited by $2 via TableEdit

{{See|Help:Strains|strains}}


{| id="A4e403ec47c5b3" class=" tableEdit Strain_Geneology_Table dataTable"

|-
!align=left {{table_heading_style}} |Name
||
MC1061
|-
!align=left {{table_heading_style}} |Synonyms
||

|-
!align=left {{table_heading_style}} |Genotype
||
araD139 Del(araA-leu)7697 Del(lac)X74 galK16 galE15(GalS) lambda- e14- mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2

This is the genotype at CGSC as of Nov, 2008. Note that there are differences with the original genotype <ref name='PMID:6997493'/>
|-
!align=left {{table_heading_style}} |Phenotype
||
streptomycin resistance
|-
!align=left {{table_heading_style}} |Plasmids
||

|-
!align=left {{table_heading_style}} |Phage
||

|-
!align=left {{table_heading_style}} |Sources
||
[http://cgsc.biology.yale.edu/Strain.php?ID=11225 CGSC] 
[https://bccm.belspo.be/page/genecorner-hosts-display-razor/257 LMBP 9560]
|-
!align=left {{table_heading_style}} |Construction
||
Made by Malcolm Casadaban
|-
!align=left {{table_heading_style}} |References
||
PMID:6997493

|- class="tableEdit_footer"
|<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.179470.A4e403ec47c5b3&page=179470&pagename={{FULLPAGENAMEE}}&type=1&template=Strain_Geneology_Table edit table]</div> ||
|}


== Notes ==
Useful host for primary transformation. Transforms very well by the CaCl2 method (107/µg). There is no Type I restriction; incoming DNA receives the E. coli K modification. 
As this strain is deleted for the lacI repressor gene, it is not a suitable host for plasmids carrying the lac promoter or derivatives thereof, such as tac, trc, N25/O2 … promoters. In the absence of repression, continuous transcription from the lac promoter is likely to result in plasmid instability. 
The zeocin resistance is most probably related to the presence of a functional recA gene (recA+).

==References==
{{RefHelp}}
<references/>

__NOTOC__
[[Category:Strains]][[Category:BCCM/GeneCorner]]

Category:Strain:Hfr 3000

2024-10-28T11:06:15Z

Wouter.deschamphelaire: Table edited by $2 via TableEdit

[[Category:Strain:HfrH Thi-]]
{{See|Help:Strains|strains}}
<protect>

{| id="W4e1741c80cbcc" class=" tableEdit Strain_Geneology_Table dataTable"

|-
!align=left {{table_heading_style}} |Name
||
Hfr3000
|-
!align=left {{table_heading_style}} |Synonyms
||
HfrH Thi- λ-; HfrC (Paris), AB259, K12 3000
|-
!align=left {{table_heading_style}} |Genotype
||
Hfr (PO1)''relA1 spoT1 thi-1''
|-
!align=left {{table_heading_style}} |Phenotype
||

|-
!align=left {{table_heading_style}} |Plasmids
||
Hfr
|-
!align=left {{table_heading_style}} |Phage
||
λ-
|-
!align=left {{table_heading_style}} |Sources
||
[http://cgsc.biology.yale.edu/Strain.php?ID=4637 CGSC:259] 
[https://bccm.belspo.be/page/genecorner-hosts-display-razor/103 LMBP 1393]
|-
!align=left {{table_heading_style}} |Construction
||
Strain Hfr 3000 is a UV treated isolate of strain HfrH Thi- 
|-
!align=left {{table_heading_style}} |References
||
PMID:5340246 PMID:4568763, PMID:4148765

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</protect>

== Notes ==
The strain is useful for propagation of F+ specific phages.

[[Category:BCCM/GeneCorner]]

==References==
{{RefHelp}}
<references/>

__NOTOC__

Category:Strains

2024-10-24T11:46:26Z

Wouter.deschamphelaire: /* See also */

This page contains links to information about various ''E. coli'' Strains.

If you were looking for genes or a list of genomes in EcoliWiki, see [[:Category:E._coli_genomes]].

We've generated different sub-categories for easier browsing and individual strains are listed below the sub-categories. Please note that this list is not all-inclusive, but merely a starting point for some of the more commonly used strains. For help on strains, please see [[Help:Strains]]. To add a new strain, visit the [[Page_Generator#Strain_Page]].

== See also ==
* [https://bccm.belspo.be/genecorner-host-search BCCM/GeneCorner Plasmid Collection host strains]
* [http://cgsc.biology.yale.edu/top.html Coli Genetic Stock Center (CGSC)]
* [[oww:Strains|Strain information at OpenWetWare]]
* [[Open Biosystems]] [http://www.openbiosystems.com/GeneExpression/Non%2DMammalian/Bacteria/ Non-mammalian sets]

DP50

2022-05-30T08:14:38Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| DP50
|-
| Synonyms
|
|-
| Genotype
| F-, fhuA53, dapD8, lacY1 or Δ(cod-lacI)6, glnX44(AS), Δ(gal-uvrB)47, λ-, gyrA29, ΔthyA57, hsdS3
|-
| Phenotype
| nalidixic acid resistant
|-
| Sources
| [https://bccm.belspo.be/about-us/bccm-genecorner BCCM/GeneCorner (strain was removed from collection)]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/322278 PMID:322278]
|}

===Notes===
This strain is used for propagation of phage lambda and phage lambda Charon.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

DP50

2022-05-30T08:13:55Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| DP50
|-
| Synonyms
|
|-
| Genotype
| F-, fhuA53, dapD8, lacY1 or Δ(cod-lacI)6, glnX44(AS), Δ(gal-uvrB)47, λ-, gyrA29, ΔthyA57, hsdS3
|-
| Phenotype
| nalidixic acid resistant
|-
| Sources
| [https://bccm.belspo.be/about-us/bccm-genecorner LMBP 8014 (strain was removed from collection)]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/322278 PMID:322278]
|}

===Notes===
This strain is used for propagation of phage lambda and phage lambda Charon.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

BL21pT7POL26

2020-10-16T13:44:24Z

Wouter.deschamphelaire: Created page with "{| class="wikitable" border="1" |- | Name | BL21pT7POL26 |- | Synonyms | BL21[pT7POL26] |- | Genotype | F- ompT gal dcm lon hsdSB(rB-mB-) [malB+]K-12(λS) |- | Phenotype | kan..."

{| class="wikitable" border="1"
|-
| Name
| BL21pT7POL26
|-
| Synonyms
| BL21[pT7POL26]
|-
| Genotype
| F- ompT gal dcm lon hsdSB(rB-mB-) [malB+]K-12(λS)
|-
| Phenotype
| kanamycin resistant
|-
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%209558 LMBP 9558]
|-
| References
|
|}

===Notes===
This strain contains the low-copy auxiliary pT7POL26 vector for T7 promoter-driven protein expression, which is repressed until IPTG induction of T7 RNA polymerase expression occurs. The strain does not contain the lon protease and is also deficient in the outer membrane protease OmpT. The lack of two key proteases reduces the degradation of heterologous proteins expressed in the strain.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

JK242

2020-10-16T13:40:10Z

Wouter.deschamphelaire: Created page with "{| class="wikitable" border="1" |- | Name | JK242 |- | Synonyms | |- | Genotype | asnA31 asnB32 thi-1 relA spoT rpsL125 |- | Phenotype | streptomycin resistant |- | Sources |..."

{| class="wikitable" border="1"
|-
| Name
| JK242
|-
| Synonyms
|
|-
| Genotype
| asnA31 asnB32 thi-1 relA spoT rpsL125
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%20917 LMBP 917]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/6780757 PMID:6780757]
|}

===Notes===
The JK242 strain is auxotrophic for asparagine because of the inactivated asnA and asnB gene. It carries also the streptomycin-resistant allele, rpsL, a mutation leading to increased translational accuracy.

[[Category:BCCM/GeneCorner]][[Category:Strains]]

BHB2688

2020-10-16T13:30:56Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| BHB2688
|-
| Synonyms
|
|-
| Genotype
| F- λ[Eam4 b2 red3 imm434 cIts Sam7] recA3 IN(rrnD-rrnE)1 rpsL200
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%208016 LMBP 8016] [https://www.lgcstandards-atcc.org/products/all/35131.aspx ATCC 35131] [https://www.dsmz.de/collection/catalogue/details/culture/DSM-5451 DSM 5451] [https://cgsc.biology.yale.edu/StrainQueryForm.php CGSC 13584]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/161604 PMID:161604]
|}

===Notes===
The strain is meant for in vitro packaging of bacteriophage lambda and cosmid DNA and is used for the preparation of freeze-thaw lysates (FTL).

[[Category:BCCM/GeneCorner]][[Category:Strains]]

BHB2688

2020-10-16T13:30:33Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| BHB2688
|-
| Synonyms
|
|-
| Genotype
| F- λ[Eam4 b2 red3 imm434 cIts Sam7] recA3 IN(rrnD-rrnE)1 rpsL200
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%208016 LMBP 8016]
[https://www.lgcstandards-atcc.org/products/all/35131.aspx ATCC 35131]
[https://www.dsmz.de/collection/catalogue/details/culture/DSM-5451 DSM 5451]
[https://cgsc.biology.yale.edu/StrainQueryForm.php CGSC 13584]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/161604 PMID:161604]
|}

===Notes===
The strain is meant for in vitro packaging of bacteriophage lambda and cosmid DNA and is used for the preparation of freeze-thaw lysates (FTL).

[[Category:BCCM/GeneCorner]][[Category:Strains]]

BHB2688

2020-10-16T13:30:12Z

Wouter.deschamphelaire:

{| class="wikitable" border="1"
|-
| Name
| BHB2688
|-
| Synonyms
|
|-
| Genotype
| F- λ[Eam4 b2 red3 imm434 cIts Sam7] recA3 IN(rrnD-rrnE)1 rpsL200
|-
| Phenotype
| streptomycin resistant
|-
| Sources
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%208016 LMBP 8016] [https://www.lgcstandards-atcc.org/products/all/35131.aspx ATCC 35131] 
[https://www.dsmz.de/collection/catalogue/details/culture/DSM-5451 DSM 5451] 
[https://cgsc.biology.yale.edu/StrainQueryForm.php CGSC 13584]
|-
| References
| [https://pubmed.ncbi.nlm.nih.gov/161604 PMID:161604]
|}

===Notes===
The strain is meant for in vitro packaging of bacteriophage lambda and cosmid DNA and is used for the preparation of freeze-thaw lysates (FTL).

[[Category:BCCM/GeneCorner]][[Category:Strains]]