https://ecoliwiki.org/colipedia/api.php?action=feedcontributions&feedformat=atom&user=Wilfredpoppinga EcoliWiki - User contributions [en] 2026-11-06T14:08:42Z User contributions MediaWiki 1.43.11 https://ecoliwiki.org/colipedia/index.php?title=pBAD/myc-His_A&diff=936234 pBAD/myc-His A 2009-09-04T08:37:43Z <p>Wilfredpoppinga: </p> <hr /> <div>==At a Glance==<br /> [[Image:PBAD MYCA.jpg|thumb|440px|right| pBAD/myc-His A, Image provided by [https://www.invitrogen.com/content/sfs/vectors/pbadmychis_map.pdf Invitrogen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U4705b357bf715--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> * <br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** <br /> * <br /> ****************************************************************************************** --&gt;<br /> {|border=&quot;2&quot; cellpadding=&quot;4&quot; cellspacing=&quot;0&quot; style=&quot;margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;&quot; {{Prettytable}} width='30%' align=left<br /> <br /> |-<br /> !align=left {{table heading style}} |Type<br /> ||<br /> Expression<br /> |-<br /> !align=left {{table heading style}} |Origin of Replication<br /> ||<br /> pBR322<br /> |-<br /> !align=left {{table heading style}} |Copy #<br /> ||<br /> <br /> |-<br /> !align=left {{table heading style}} |Markers<br /> ||<br /> Ampicillin<br /> |-<br /> !align=left {{table heading style}} |Link to Sequence<br /> ||<br /> [https://www.invitrogen.com/content/sfs/vectors/pbadmychisa_seq.txt Invitrogen]<br /> |-<br /> !align=left {{table heading style}} |Features<br /> ||<br /> araBAD promoter <br /> <br /> Polyhistidine tag<br /> <br /> Enterokinase recogniton site<br /> <br /> MCS (multiple cloning site)<br /> <br /> AraC ORF<br /> <br /> <br /> |-<br /> !align=left {{table heading style}} |Accession Number<br /> ||<br /> <br /> <br /> |-class='sortbottom'<br /> |[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.U4705b357bf715&amp;page=0&amp;pagename={{FULLPAGENAMEE}}&amp;type=1&amp;template=PlasmidTable edit table] ||<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U4705b357bf715--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> The pBAD/Myc-His Kit provides all of the reagents to express your protein in a tightly regulated fashion. The pBAD/Myc-His vector expresses native proteins or fusion proteins with a C-terminal tag. The vector provides the araBAD promoter for tightly regulated expression, translation initiation signals optimized for E. coli expression, C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with an Anti-His(C-term) Antibody, and C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody<br /> <br /> Three vectors are provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene. pBAD/myc-His A does not contain ''Sna''B 1 or ''Xba'' 1.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources==<br /> [https://catalog.invitrogen.com/index.cfm?fuseaction=viewCatalog.viewProductDetails&amp;productDescription=612 Ivitrogen]<br /> <br /> ==References==<br /> {{BacktoTop}}<br /> {{RefHelp}}<br /> &lt;references /&gt;<br /> &lt;ref name='PMID:3915782'/&gt;<br /> &lt;ref name='PMID:7608087'/&gt;<br /> &lt;ref name='PMID:2661528'/&gt;<br /> ==Sequence==<br /> AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAAC<br /> CAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGT<br /> AACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATG<br /> CCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTC<br /> TCCATACCCGTTTTTTGGGCTAACAGGAGGAATTAACCATGGATCCGAGCTCGAGATCTGCAGCTGGTAC<br /> CATATGGGAATTCGAAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGA<br /> CCATCATCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTT<br /> TCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAG<br /> CGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGG<br /> TCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCC<br /> TTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGA<br /> ACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAA<br /> GCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACA<br /> TTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT<br /> ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTC<br /> ACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACT<br /> GGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTT<br /> AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATAC<br /> ACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT<br /> AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATC<br /> GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGG<br /> AACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAAC<br /> GTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG<br /> GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG<br /> GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGT<br /> AGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC<br /> TCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTC<br /> ATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGA<br /> GTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTG<br /> CGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGC<br /> TACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTA<br /> GCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTA<br /> CCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATA<br /> AGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGA<br /> ACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTAT<br /> CCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTT<br /> ATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAG<br /> CCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG<br /> TTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTC<br /> GCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTT<br /> TCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCC<br /> GCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCC<br /> AACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTC<br /> TCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGC<br /> GCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATG<br /> CTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACT<br /> TTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAAT<br /> AGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAG<br /> CTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGA<br /> TGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCA<br /> GGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAAT<br /> CGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCC<br /> CCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAA<br /> AGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTA<br /> AACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAAC<br /> AGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGG<br /> TGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTC<br /> AATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCT<br /> TCAGCCATACTTTTCATACTCCCGCCATTCAGAG<br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Invitrogen Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:MCS]][[Category:araBAD Promoter]][[Category:Polyhistidine tag]]<br /> __NOTOC__</div> Wilfredpoppinga https://ecoliwiki.org/colipedia/index.php?title=pET-15b&diff=928762 pET-15b 2009-08-23T10:38:14Z <p>Wilfredpoppinga: Editing code to make image visible</p> <hr /> <div>==At a Glance==<br /> [[Image:PET-15b.jpg|thumb|450px|right|pET-15b Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U46f2eb0192727--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> * <br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** <br /> * <br /> ****************************************************************************************** --&gt;<br /> {|border=&quot;2&quot; cellpadding=&quot;4&quot; cellspacing=&quot;0&quot; style=&quot;margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;&quot; {{Prettytable}} width='30%' align=left<br /> <br /> |-<br /> !align=left {{table heading style}} |Type<br /> ||<br /> Cloning/Expression<br /> |-<br /> !align=left {{table heading style}} |Origin of Replication<br /> ||<br /> pBR322<br /> |-<br /> !align=left {{table heading style}} |Copy #<br /> ||<br /> ~40<br /> |-<br /> !align=left {{table heading style}} |Markers<br /> ||<br /> Ampicillin<br /> |-<br /> !align=left {{table heading style}} |Link to Sequence<br /> ||<br /> [http://www.emdbiosciences.com/docs/NDIS/69661-000.html Novagen]<br /> |-<br /> !align=left {{table heading style}} |Features<br /> ||<br /> T7 Promoter &lt;ref name='PMID:16236800'/&gt;&lt;br&gt;<br /> T7 transcription start &lt;br&gt;<br /> His-Tag &lt;ref name='PMID:16236800'/&gt;&lt;ref name='PMID:17507374'/&gt;&lt;ref name='PMID:17383962'/&gt;&lt;ref name='PMID:17158871'/&gt;&lt;ref name='PMID:17355978'/&gt; &lt;br&gt;<br /> MCS &lt;br&gt;<br /> T7 Terminator &lt;br&gt;<br /> lacI &lt;br&gt;<br /> |-<br /> !align=left {{table heading style}} |Accession Number<br /> ||<br /> <br /> <br /> |-class='sortbottom'<br /> |[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.U46f2eb0192727&amp;page=0&amp;pagename={{FULLPAGENAMEE}}&amp;type=1&amp;template=PlasmidTable edit table] ||<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U46f2eb0192727--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> The pET-15b&lt;ref name='PMID:16236800'/&gt;&lt;ref name='PMID:17507374'/&gt;&lt;ref name='PMID:17383962'/&gt;&lt;ref name='PMID:17158871'/&gt;&lt;ref name='PMID:17355978'/&gt;&lt;ref name='PMID:17298945'/&gt; vector possesses an His•Tag at the N-terminus, a subsequent [thrombin cleavage site]&lt;ref name='PMID:17507374'/&gt; and three cloning sites.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources==<br /> [http://www.emdbiosciences.com/product/69661#citations Novagen]<br /> <br /> ==References==<br /> {{BacktoTop}}<br /> {{RefHelp}}<br /> &lt;references /&gt;<br /> &lt;ref name='PMID:16236800'/&gt;<br /> &lt;ref name='PMID:17507374'/&gt;<br /> &lt;ref name='PMID:17383962'/&gt;<br /> &lt;ref name='PMID:17158871'/&gt;<br /> &lt;ref name='PMID:17355978'/&gt;<br /> &lt;ref name='PMID:17298945'/&gt;<br /> ==Sequence==<br /> <br /> 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa <br /> 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg <br /> 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt <br /> 181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag <br /> 241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt <br /> 301 cgggctttgt tagcagccgg atcctcgagc atatggctgc cgcgcggcac caggccgctg <br /> 361 ctgtgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa <br /> 421 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa <br /> 481 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc <br /> 541 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct <br /> 601 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc <br /> 661 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac <br /> 721 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga <br /> 781 gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga <br /> 841 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta <br /> 901 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc <br /> 961 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt <br /> 1021 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc <br /> 1081 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc <br /> 1141 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca <br /> 1201 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga <br /> 1261 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga <br /> 1321 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga <br /> 1381 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt <br /> 1441 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc <br /> 1501 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat <br /> 1561 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg <br /> 1621 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg <br /> 1681 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga <br /> 1741 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc <br /> 1801 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc <br /> 1861 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca <br /> 1921 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc <br /> 1981 attaggcacc gggatctcga ccgatgccct tgagagcctt caacccagtc agctccttcc <br /> 2041 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac <br /> 2101 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga <br /> 2161 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag <br /> 2221 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca <br /> 2281 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct <br /> 2341 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc <br /> 2401 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta <br /> 2461 ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga <br /> 2521 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg <br /> 2581 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct <br /> 2641 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa <br /> 2701 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac <br /> 2761 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc <br /> 2821 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat <br /> 2881 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg <br /> 2941 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac <br /> 3001 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc <br /> 3061 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca <br /> 3121 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac <br /> 3181 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc <br /> 3241 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt <br /> 3301 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca <br /> 3361 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc <br /> 3421 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc <br /> 3481 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg <br /> 3541 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt <br /> 3601 aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac <br /> 3661 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg <br /> 3721 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa <br /> 3781 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc <br /> 3841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc <br /> 3901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat <br /> 3961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc <br /> 4021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct <br /> 4081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg <br /> 4141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc <br /> 4201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga <br /> 4261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa <br /> 4321 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta <br /> 4381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc <br /> 4441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg <br /> 4501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga <br /> 4561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg <br /> 4621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct <br /> 4681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg <br /> 4741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc <br /> 4801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa <br /> 4861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc <br /> 4921 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt <br /> 4981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc <br /> 5041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt <br /> 5101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc <br /> 5161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt <br /> 5221 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata <br /> 5281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga <br /> 5341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag <br /> 5401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa <br /> 5461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt <br /> 5521 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga <br /> 5581 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag <br /> 5641 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc <br /> 5701 ttcaagaa<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]][[Category:His Tag]][[Category:lacI]][[Category:MCS]]<br /> __NOTOC__</div> Wilfredpoppinga https://ecoliwiki.org/colipedia/index.php?title=User:Wilfredpoppinga&diff=922158 User:Wilfredpoppinga 2009-08-12T16:38:00Z <p>Wilfredpoppinga: Created page with &#039;==Contact Info== *Wilfred J. Poppinga *[http://www.rug.nl/corporate/index University of Groningen] *Groningen, The Netherlands. *[mailto:W.J.Poppinga@student.rug.nl E-mail me]&lt;B...&#039;</p> <hr /> <div>==Contact Info==<br /> *Wilfred J. Poppinga<br /> *[http://www.rug.nl/corporate/index University of Groningen]<br /> *Groningen, The Netherlands. <br /> *[mailto:W.J.Poppinga@student.rug.nl E-mail me]&lt;BR&gt;<br /> <br /> I currently work in co-operation with the [http://molgen.biol.rug.nl/molgen/index.php Molecular Genetics] Lab at [http://www.rug.nl/corporate/index the Groninger University] during the [http://www.igem.org/ iGEM competition]. <br /> <br /> ==Education==<br /> &lt;!--Include info about your educational background--&gt;<br /> &lt;!--* Year, PhD, Institute--&gt;<br /> * 2010, Master of Science, [http://www.rug.nl/levenswetenschappen/informatievoor/studiekiezers_msc/iv_mfw/index?lang=en Medical Pharmaceutical Sciences] @ [http://www.rug.nl/corporate/index The University of Groningen] <br /> * 2008, Bachelor of Applied Science, [http://en.wikipedia.org/wiki/Biotechnology Biotechnology] @ [http://www.vanhall-larenstein.com/ the University of Applied Science Van Hall Larenstein] and [http://www.nhl.nl/nhl_nl/informatie_voor/foreign_visitor/ the NHL University of Applied Science]<br /> <br /> ==Research interests==<br /> &lt;!-- Feel free to add brief descriptions to your research interests as well --&gt;<br /> # Synthetic biology<br /> # Molecular biology <br /> # Pharmaceutical biology<br /> # (Molecular) Microbiology <br /> # Medical biology<br /> # Pathology<br /> &lt;center&gt;&lt;i&gt;[http://en.wikipedia.org/wiki/West_Frisian_language Folle nocht en wille kin een protte tille]&lt;/i&gt;&lt;/center&gt;<br /> <br /> <br /> &lt;!-- ==Publications==<br /> Replace the PubMed ID's (&quot;pmid=#######&quot;) below with the PubMed ID's for your publications. You can add or remove lines as needed &lt;biblio&gt;<br /> #Paper1 pmid=6947258<br /> #Paper2 pmid=13718526<br /> // leave a comment about a paper here<br /> #Book1 isbn=0879697164<br /> &lt;/biblio&gt;--&gt;</div> Wilfredpoppinga