EcoliWiki - User contributions [en]

PMID:7007352

2008-05-01T08:23:34Z

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 '''Beutin, L, Manning, PA, Achtman, M and Willetts, N''' (1981) sfrA and sfrB products of Escherichia coli K-12 are transcriptional control factors.''J. Bacteriol.'' '''145''':840-4

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The mechanisms whereby mutations in Escherichia coli K-12 genes sfrA and sfrB reduce expression of the transfer functions of sex factor F have been examined by assaying the levels of tra messenger ribonucleic acid and of tra proteins. The sfrA product was necessary for efficient transcription of the control gene traJ and, directly or indirectly, for transcription of the traY leads to Z operon. In the absence of sfrA, reduced levels of the traJ and traT proteins were observed in the outer membrane. The sfrB product was needed to prevent premature transcription at one or more rho-dependent termination sites. sfrB mutations also reduced synthesis of full-length lipopolysaccharide molecules, of several chromosomally determined outer membrane proteins, and of functional flagella. Thus, the sfrB product may act as an antiterminator in transcription of several operons encording cell envelope components.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7007352 PubMed]
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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PMID:2695826

2008-05-01T08:23:20Z

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 '''Betzner, AS and Keck, W''' (1989) Molecular cloning, overexpression and mapping of the slt gene encoding the soluble lytic transglycosylase of Escherichia coli.''Mol. Gen. Genet.'' '''219''':489-91

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The gene of the major autolysin of Escherichia coli, the soluble lytic transglycosylase (Slt), was isolated from an expression gene library. The cloned slt gene was used to determine its chromosomal map position adjacent to trp R at 99.7 min on the E. coli linkage map.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2695826 PubMed]
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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PMID:3732806

2008-05-01T08:23:06Z

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 '''Betz, JL''' (1986) Cloning and characterization of several dominant-negative and tight-binding mutants of lac repressor.''Gene'' '''42''':283-92

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|{{TableHeadingColor}} |'''Abstract''' || <protect>Using both general recombination and molecular cloning techniques, 13I-, I-d and Itb missense mutations in the lacI gene were transferred from F'lacIq episomes to ColE1 derivative plasmids. Two deletion derivatives of the lacI genes encoding the wild-type (wt) and the tight-binding (Itb) B3 and B5 repressors were also constructed. The mutant repressors were examined for polypeptide size and stability, and for binding to the inducer isopropyl-beta-D-thiogalactoside (IPTG). Several of the I-d repressors were shown to be partially degraded in vivo, in confirmation of earlier results based on [14C]IPTG binding [Miwa and Sadler, J. Mol. Biol. 117 (1977) 843-868]. The sizes of polypeptides produced by lacI deletion derivatives were consistent with expectations based on the extent of deletion and the location of termination sites within the plasmid sequence. The first 400 bp of several mutant lacI genes were sequenced. Our wt lacI gene differs from another wt lacI sequence (Farabaugh, 1978), containing a single bp change that results in an Ala to Thr substitution at amino acid (aa) 109. We identified bp substitutions and the resultant aa changes for two Itb and two I-d genes; the positions correlated with prior genetic mapping data. Three of these new changes were in the N-terminal domain (headpiece) of repressor, with one change in the core domain at aa 99.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3732806 PubMed]
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PMID:8626487

2008-05-01T08:21:40Z

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 '''Betton, JM and Hofnung, M''' (1996) Folding of a mutant maltose-binding protein of Escherichia coli which forms inclusion bodies.''J. Biol. Chem.'' '''271''':8046-52

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The maltose-binding protein (MalE) of Escherichia coli is the periplasmic component of the transport system for malto-oligosaccharides. We have examined the characteristics of a Mal- mutant of malE corresponding to the double substitution Gly32 --> Asp/Ile33 --> Pro, MalE31, previously obtained by random mutagenesis. In vivo, the MalE31 precursor is efficiently processed, but the mature protein forms inclusion bodies in the periplasm. Furthermore, the accumulation of insoluble MalE31 is independent of its cellular localization; MalE31 lacking its signal sequence forms inclusion bodies in the cytoplasm. The native MalE31 protein can be purified by affinity chromatography from inclusion bodies after denaturation by 8 M urea. The renatured protein exhibits full maltose binding affinity (Kd= 9 x 10(-7) M), suggesting that its folded structure is similar to that of the wild-type protein. Unfolding/refolding experiments show that MalE31 is less stable (-5. 5 kcal/mol) than the wild-type protein (-9.5 kcal/mol) and that folding intermediates have a high tendency to form aggregates. In conclusion, the observed phenotype of cells expressing malE31 can be explained by a defective folding pathway of the protein.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8626487 PubMed]
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PMID:4381707

2008-05-01T08:21:25Z

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 '''Bertino, JB and Stacey, KA''' (1966) A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli.''Biochem. J.'' '''101''':32C-33C

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|{{TableHeadingColor}} |'''Abstract''' || <protect>No abstract in PubMed</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4381707 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241946'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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PMID:8071234

2008-05-01T08:21:10Z

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 '''Bertin, P, Terao, E, Lee, EH, Lejeune, P, Colson, C, Danchin, A and Collatz, E''' (1994) The H-NS protein is involved in the biogenesis of flagella in Escherichia coli.''J. Bacteriol.'' '''176''':5537-40

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The function of the flagellum-chemotaxis regulon requires the expression of many genes and is positively regulated by the cyclic AMP-catabolite activator protein (cAMP-CAP) complex. In this paper, we show that motile behavior was affected in Escherichia coli hns mutants. The loss of motility resulted from a complete lack of flagella. A decrease in the level of transcription of the flhD and fliA genes, which are both required for the synthesis of flagella, was observed in the presence of an hns mutation. Furthermore, the Fla- phenotype was not reversed to the wild type in the presence of a cfs mutation which renders the flagellum synthesis independent of the cAMP-CAP complex. These results suggest that the H-NS protein acts as a positive regulator of genes involved in the biogenesis of flagella by a mechanism independent of the cAMP-CAP pathway.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8071234 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241945'
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PMID:8389475

2008-05-01T08:20:56Z

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 '''Bernstein, HD, Zopf, D, Freymann, DM and Walter, P''' (1993) Functional substitution of the signal recognition particle 54-kDa subunit by its Escherichia coli homolog.''Proc. Natl. Acad. Sci. U.S.A.'' '''90''':5229-33

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The 54-kDa subunit of the mammalian signal recognition particle (SRP54) binds to the signal sequences of nascent secretory and transmembrane proteins and facilitates their cotranslational targeting to the membrane translocation apparatus in the endoplasmic reticulum (ER). A 48-kDa Escherichia coli protein that shares extensive sequence similarity with SRP54 was identified in homology searches. Recent genetic experiments by Phillips and Silhavy [Phillips, G. J. & Silhavy, T. J. (1992) Nature (London) 359, 744-746] have shown that depletion of this protein, designated Ffh (fifty-four homolog), leads to a significant secretory defect in vivo. We demonstrate here that Ffh is structurally and functionally related to SRP54 by virtue of its ability to mimic closely its mammalian counterpart in several established biochemical assays, thereby suggesting that it plays a direct role in protein export. Ffh assembled efficiently with mammalian SRP components into a chimeric ribonucleoprotein ["SRP(Ffh)"] and bound at the site normally occupied by SRP54. Like SRP54, the Ffh moiety of the chimeric particle specifically recognized the signal sequence of preprolactin in a photocrosslinking assay. Moreover, Ffh could also act in concert with other SRP components to arrest elongation of preprolactin upon recognition of the signal sequence. In all of these assays, Ffh had approximately the same specific activity as SRP54. In contrast, SRP(Ffh) did not promote the translocation of preprolactin across the membrane of microsomal vesicles, suggesting that Ffh cannot mediate an interaction with a membrane component that is required for the translocation of nascent chains.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8389475 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241944'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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PMID:4934061

2008-05-01T08:20:41Z

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 '''Berman-Kurtz, M, Lin, EC and Richey, DP''' (1971) Promoter-like mutant with increased expression of the glycerol kinase operon of Escherichia coli.''J. Bacteriol.'' '''106''':724-31

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|{{TableHeadingColor}} |'''Abstract''' || <protect>A glycerol-specific phenotypic revertant isolated from a mutant of Escherichia coli missing enzyme I of the phosphoenolpyruvate phosphotransferase system was studied. This revertant is capable of producing higher levels of glycerol kinase and the protein mediating the facilitated diffusion of glycerol (facilitator) than wild-type cells. The kinase of the revertant is indistinguishable from the wild-type enzyme with respect to its sensitivity to feedback inhibition by fructose-1,6-diphosphate, its pH optimum, and its turnover number. The synthesis of glycerol kinase in strains bearing the suppressor locus is resistant to catabolite repression. The suppressor mutation mapped at the known glpK locus. Thus, it is suggested that the mutation occurred in the promoter of the operon specifying the kinase and the facilitator.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4934061 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241943'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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PMID:6260755

2008-05-01T08:19:14Z

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 '''Berg, PE''' (1981) Cloning and characterization of the Escherichia coli gene coding for alkaline phosphatase.''J. Bacteriol.'' '''146''':660-7

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The Escherichia coli structural gene for alkaline phosphatase, phoA, and a promoter-like mutant of phoA, called pho-1003(Bin) phoA+, were cloned by using plasmid vectors. Initially, these genes were cloned on deoxyribonucleic acid fragments of 28.9 kilobases (kb). Subsequently, they were subcloned on fragments and 4.8 and then 2.7 kilobases. A restriction map was developed, and phoA was localized to a 1.7-kb region. The promoter end of the gene was inferred by its proximity to another gene cloned on the same deoxyribonucleic acid fragment, proC. The stability of the largest plasmid (33.3 kb) was found to be recA dependent, although the subcloned plasmids were stable in a recA+ strain. Synthesis of alkaline phosphatase directed by the phoA+ and pho-1003(Bin) phoA+ plasmids in a phoA deletion strain was assayed under repressing and derepressing levels of phosphate. These data were compared with the copy numbers of the plasmids. It was found that synthesis of alkaline phosphatase was tightly regulated, even under derepressing conditions: a copy number of 17 enabled cells to synthesize only about twofold more enzyme than did cells with 1 chromosomal copy of phoA+. Enzyme levels were also compared for cells containing pho-1003(Bin) phoA+ and phoA+.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6260755 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241942'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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PMID:2435709

2008-05-01T08:18:59Z

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 '''Berg, KL, Squires, CL and Squires, C''' (1987) In vivo translation of a region within the rrnB 16S rRNA gene of Escherichia coli.''J. Bacteriol.'' '''169''':1691-701

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|{{TableHeadingColor}} |'''Abstract''' || <protect>In this study we show that a segment of the Escherichia coli rrnB 16S gene can be translated in vivo. Other laboratories have previously reported that there are internal transcription and translation signals and open reading frames within the E. coli rrnB rRNA operon. Their studies revealed a translation start signal followed by a 252-base-pair open reading frame (ORF16) within the 16S gene and detected a promoter (p16) in the same general region by using in vitro RNA polymerase binding and transcription initiation assays. By using plasmid gene fusions of ORF16 to lacZ we showed that an ORF16'-'beta-galactosidase fusion protein was made in vivo. Transcripts encoding the fusion protein were expressed either from the rrnB p1p2 control region or from a hybrid trp-lac promoter (tacP), but the amount of expression was considerably less than for a lacZ control plasmid. We used fusions to the cat gene to show that p16 is one-half as active as lacP. Deletions were used to show that p16 is located within ORF16 and thus cannot promote a transcript encoding the ORF16 peptide. A comparison of sequences from different organisms shows that ORF16 and p16 lie in a highly conserved region of the procaryotic 16S RNA structure. The first 20 amino acids of ORF16 are conserved in most eubacterial and plant organellar sequences, and promoter activity has been detected in this region of the Caulobacter crescentus sequence by other workers.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2435709 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241941'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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PMID:395023

2008-05-01T08:18:43Z

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 '''Berg, CM, Shaw, KJ, Vender, J and Borucka-Mankiewicz, M''' (1979) Physiological characterization of polar Tn5-induced isoleucine-valine auxotrophs in Escherichia coli K.12: evidence for an internal promoter in the ilvOGEDA operon.''Genetics'' '''93''':308-19

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The properties of 22 isoleucine-valine auxotrophs induced in Escherichia coli K-12 by the transposable element, Tn5, were characterized on the basis of growth requirements, cross-feeding behavior, and enzyme activity. Mutants defective in ilvA, ilvC, ilvD and ilvE were found. Mutation in ilvE were not completely polar on ilvD and ilvA enzyme activities (that is, ilvE mutants possessed a low constitutive level of expression of the enzymes coded by ilvD and ilvA), while mutations in ilvD were completely polar on ilvA enzyme activity. The data suggest that there is an internal promoter between the sites of Tn5 insertion in ilvE and ilvD.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=395023 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241940'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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PMID:6260586

2008-05-01T08:18:29Z

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 '''Berg, CM, Shaw, KJ and Berg, DE''' (1980) The ilvG gene is expressed in Escherichia coli K-12.''Gene'' '''12''':165-70

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|{{TableHeadingColor}} |'''Abstract''' || <protect>Three genes code for isozymes of acetohydroxy acid synthase (AHAS) in Escherichia coli K-12. To test the previously published supposition that one of them, ilvG, is silent in ilvO+ strains, we isolated mutants which had deletions of various lengths in the ilvGEDA operon. Some of these mutants have severely reduced levels of AHAS activity. We conclude that ilvG is expressed in ilvO+ strains but is deleted in these mutants. In addition, we find that AHAS II, the ilvG gene product, is sensitive to feedback inhibition by valine. We hypothesize that ilvO- mutations are ilvG frameshift mutations which render AHAS II valine resistant and enhance transcription of distal genes.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6260586 PubMed]
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PMID:4598010

2008-05-01T08:18:16Z

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 '''Berg, CM and Rossi, JJ''' (1974) Proline excretion and indirect suppression in Escherichia coli and Salmonella typhimurium.''J. Bacteriol.'' '''118''':928-34

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess Delta(1)-pyrroline-5-carboxylate, the immediate precursor of proline, is added to a culture. In proline auxotrophs blocked at an early step in proline biosynthesis (proA or proB), reversion to prototrophy is often due to a mutation in the arginine pathway which diverts N-acetyl glutamate gamma-semialdehyde to proline synthesis, thus bypassing the proA or proB block. In such double mutants (proAB, argD), the last step in proline synthesis appears to be unregulated, since proline is excreted. Feedback inhibition and repression of the arginine pathway overcomes indirect suppression (restoring the Pro(-) phenotype), but proline regulation is not restored; double mutants still excrete proline when fed Delta(1)-pyrroline-5-carboxylate exogeneously. A new class of proline analogue-resistant mutant, due to mutation at argD, is also described.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4598010 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241938'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.A48197cc7cede4&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:3527871

2008-05-01T08:13:55Z

Trap18: New PMID: Page!

{{RightTOC}}

 '''Armengod, ME and Lambíes, E''' (1986) Overlapping arrangement of the recF and dnaN operons of Escherichia coli; positive and negative control sequences.''Gene'' '''43''':183-96

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The recF gene of Escherichia coli controls one of the recombination pathways and UV sensitivity, but its precise function and expression pattern are still largely unknown. We have characterized the promoter region of the recF gene by mapping for E. coli RNA polymerase binding sites, in vitro transcription experiments, cloning, and S1 mapping of in vivo mRNAs. It contains three overlapping promoters, two initiating transcription towards recF and one in the opposite direction. The recF promoter region is located about 600 bp upstream from the start codon of the recF structural gene and resides entirely within the translated region of the preceding gene, dnaN, which encodes for the beta subunit of DNA polymerase III. This unusual arrangement might provide discoordinate regulation of the recF and dnaN genes, thus controlling the level of DNA polymerase III holoenzyme. Expression of recF is also negatively controlled by sequences located upstream as well as inside the recF coding frame. Such negative regulation may serve to prevent toxic effects due to accumulation of an excessive number of copies of the recF gene product.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3527871 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241937'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.H48197bc25f87c&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:7896685

2008-05-01T08:12:53Z

Trap18: New PMID: Page!

{{RightTOC}}

 '''Ariza, RR, Li, Z, Ringstad, N and Demple, B''' (1995) Activation of multiple antibiotic resistance and binding of stress-inducible promoters by Escherichia coli Rob protein.''J. Bacteriol.'' '''177''':1655-61

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Multiple antibiotic resistance in Escherichia coli can be mediated by induction of the SoxS or MarA protein, triggered by oxygen radicals (in the soxRS regulon) or certain antibiotics (in the marRAB regulon), respectively. These small proteins (SoxS, 107 residues; MarA, 127 residues) are homologous to the C terminus of the XylS-AraC family of proteins and are more closely related to a approximately 100-residue segment in the N terminus of Rob protein, which binds the right arm of the replication origin, oriC. We investigated whether the SoxS-MarA homology in Rob might extend to the regulation of some of the same inducible genes. Overexpression of Rob indeed conferred multiple antibiotic resistance similar to that known for SoxS and MarA (against chloramphenicol, tetracycline, nalidixic acid, and puromycin), as well as resistance to the superoxide-generating compound phenazine methosulfate. The Rob-induced antibiotic resistance depended only partially on the micF antisense RNA that down-regulates the OmpF outer membrane porin to limit antibiotic uptake. Similar antibiotic resistance was conferred by expression of a Rob fragment containing only the N-terminal 123 residues that constitute the SoxS-MarA homology. Both intact Rob and the N-terminal fragment activated expression of stress genes (inaA, fumC, sodA) but with a pattern distinct from that found for SoxS and MarA. Purified Rob protein bound a DNA fragment containing the micF promoter (50% bound at approximately 10(-9) M Rob) as strongly as it did oriC, and it bound more weakly to DNA containing the sodA, nfo, or zwf promoter (50% bound at 10(-8) to 10(-7) M). Rob formed multiple DNA-protein complexes with these fragments, as seen previously for SoxS. These data point to a DNA-binding gene activator module used in different protein contexts.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7896685 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241936'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.H48197b84bc928&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

EcoliWiki:Literature Team Comments

2008-04-30T01:33:26Z

Trap18: /* Katrina Trapp, Spring 2008 */

Below are comments provided by the first team of students to add reference articles to the EcoliWiki related to the linkage map of E.coli K-12. Since April of 2007 they have added ___ articles.

 

== Miriam Garcia, Lab Curator Fall 2007/Spring 2008 ==

== Matthew Lakich, Fall 2007/Spring 2008 ==

== Aaron Hopkins, Spring 2008 ==

== Joseph Kim, Spring 2008 ==
Besides some Internet connection hardships I experienced on my end, the literature pages and reference articles were fairly easy to get through once I got the hang of it. The use of Google Docs worked well here and I was able to get done fairly quickly and leave time for other work. Ordering pdf's of articles not available was a breeze also. Overall it was an enjoyable experience.

== Katrina Trapp, Spring 2008 ==
This task I found much easier than editing the wiki pages. It only took a few trys for me to get the hang of it. Everything went pretty smoothly and I thought that use of the Googledocs was a fantastic idea. It was easy to organize and to see the work that we had already accomplished. Overall this was a smooth and easy experience.

== Pei Yap, Spring 2008 ==

[[Category:Public Team Pages]]

EcoliWiki:Team Plasmid Comments

2008-04-30T01:31:07Z

Trap18: /* Katrina Trapp, Spring 2008 */

Below are comments provided by the first team of students to input plasmid content for the EcoliWiki. Since September of 2007 they have added 326 plasmids with 121 subcategories. For plasmids for which companies did not provide maps for, students created the maps using Gene Construction Kit.

 

== Miriam Garcia, Lab Curator Fall 2007/Spring 2008 ==

 
== Matthew Lakich, Fall 2007/Spring 2008 ==
Working on the Wiki was really easy once you learned how to make edits and changes. Sometimes information was hard to find due in part to the limited resources some of the companies provided. Overall my experience working with Team Plasmid on the EcoliWiki was a great one. One piece of advice I'd give to future workers would be to never get behind.

== Stephanie Michinock, Fall 2007 ==
The wiki is very easy to navigate once you get used to it. Entering information is not difficult, but relevant information can be hard to find. Your first page may seem very confusing, as it was for me. I would suggest that someone sit down with you for the first page, help you edit it, and then you can reference the first one as you do new pages. I know that was really helpful for me. But overall, I enjoyed working on it.

== Amy Rabb, Fall 2007 ==

== Aaron Hopkins, Spring 2008 ==

== Joseph Kim, Spring 2008 ==
Editing and navigating the wiki pages was easy once I got used to it. Finding and entering information and making sure that it was all correct was fairly easy, although some times the information was not as readily available as others. Often, I found that the references listed did not have very pertinent information but that was to be expected. Overall, it was a fluid experience.

== Katrina Trapp, Spring 2008 ==
I thought that at first the pages were a bit difficult to navigate and get used to. Once I did a few it became easier and almost second nature. Hopefully all the information that I posted was correct and useful in the future. It was also a bit mind numbing after a while because we were doing the same thing over and over again. A change of pace would have been nice a little sooner in the semester. I kind of got behind a little bit on some of my weeks and it was a task to catch up. Overall I would say that this was a learning experience. This was my first research experience and if I would give any advice it would be to keep up with your work and try to learn something from what you are doing.

== Pei Yap, Spring 2008 ==

[[Category:Public Team Pages]]

EcoliWiki:Team Plasmid Comments

2008-04-30T01:29:21Z

Trap18: /* Katrina Trapp, Spring 2008 */

Below are comments provided by the first team of students to input plasmid content for the EcoliWiki. Since September of 2007 they have added 326 plasmids with 121 subcategories. For plasmids for which companies did not provide maps for, students created the maps using Gene Construction Kit.

 

== Miriam Garcia, Lab Curator Fall 2007/Spring 2008 ==

 
== Matthew Lakich, Fall 2007/Spring 2008 ==
Working on the Wiki was really easy once you learned how to make edits and changes. Sometimes information was hard to find due in part to the limited resources some of the companies provided. Overall my experience working with Team Plasmid on the EcoliWiki was a great one. One piece of advice I'd give to future workers would be to never get behind.

== Stephanie Michinock, Fall 2007 ==
The wiki is very easy to navigate once you get used to it. Entering information is not difficult, but relevant information can be hard to find. Your first page may seem very confusing, as it was for me. I would suggest that someone sit down with you for the first page, help you edit it, and then you can reference the first one as you do new pages. I know that was really helpful for me. But overall, I enjoyed working on it.

== Amy Rabb, Fall 2007 ==

== Aaron Hopkins, Spring 2008 ==

== Joseph Kim, Spring 2008 ==
Editing and navigating the wiki pages was easy once I got used to it. Finding and entering information and making sure that it was all correct was fairly easy, although some times the information was not as readily available as others. Often, I found that the references listed did not have very pertinent information but that was to be expected. Overall, it was a fluid experience.

== Katrina Trapp, Spring 2008 ==
I thought that at first the pages were a bit difficult to navigate and get used to. Once I did a few it became easier and almost second nature. Hopefully all the information that I posted was correct and useful in the future. It was also a bit mind numbing after a while because we were doing the same thing over and over again. A change of pace would have been nice a little sooner in the semester.

== Pei Yap, Spring 2008 ==

[[Category:Public Team Pages]]

EcoliWiki:Tracking Plasmids

2008-03-26T18:35:11Z

Trap18: edited by Trap18 via TableEdit

This is a page to track plasmids entered into the wiki. It is for developmental purposes only.

<protect>

{|class = 'sortable' {{Prettytable}} id='220572'
|-
!Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed!!Date Info Added!!Date Reviewed
|-
|
LITMUS 28i
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.03.07
|
11.12.07
|
11.13.07
|-
|
LITMUS 38i
|
NEB
|
Miriam Garcia
|
08.31.2007
|
Miriam Garcia
|
08.31.07
|
10.31.07
|
10.31.07
|-
|
pGPS1.1
|
NEB
|
Charner Williams
|
08.31.07
|
Miriam Garcia
|
09.04.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pGPS2.1
|
NEB
|
Matthew Lakich
|
09.04.07
|
Miriam Garcia
|
09.06.07
|
12.04.07
|
12.17.07
|-
|
pGPS3
|
NEB
|
Grant Richardson
|
09.07.07
|
Miriam Garcia
|
09.10.07
|
Aaron Hopkins
01.22.08
|
01.23.08
|-
|
pGPS4
|
NEB
|
Amy Rabb
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
Joseph Kim
01.22.08
|
01.23.08
|-
|
pGPS5
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
11.12.07
|
11.13.07
|-
|
pMAL-c4E
|
NEB
|
Amy Rabb
|
09.10.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.21.08
|
01.23.08
|-
|
pMAL-c4G
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.31.08
|
11.13.07
|-
|
pMAL-c4X
|
NEB
|
Matthew Lakich
|
9.13.07
|
Miriam Garcia
|
09.14.07
|
12.17.07 Miriam Garcia
|
12.17.07
|-
|
pMAL-p4E
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.07.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4G
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4X
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-pIII
|
NEB
|
Charner Williams
|
09.08.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pNEB193
|
NEB
|
CharnerWilliams
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pNEB206A
|
NEB
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 09.19.07
|
09.19.07
|-
|
pTWIN1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB3
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.12.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB11
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pUC19
|
NEB
|

|

|
Miriam Garcia
|
09.26.07
|
Aaron Hopkins
|

|-
|
pNEBR-R1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1GLuc
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1Hygro
|
NEB
|
Grant Richardson
|
09.10.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1-malE
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pCMV-GLuc
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pGLuc-Basic Vector
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-Q
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-Q
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-SET1
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET1-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SET1
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SET2
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEc-SET3
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1a
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1b
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.30.08
|-
|
pBEn-SBP-SET1c
|
Stratagene
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBPa
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPb
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPc
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pBEn-SET1a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.24.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-kc
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n-EK
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pCAL-n-FLAG
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pET-11a
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11b
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
02.05.08
|
02.07.08
|-
|
pET-11c
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11d
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3a
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.04.08
|
02.13.08
|-
|
pET-3b
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3c
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3d
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET100/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
09.10.07
|

|

|
11.01.07
|

|-
|
pET-14b
|
Novagen
|
Miriam Garcia
|
09.19.07
|

|

|
11.07.07
|

|-
|
pET-15b
|
Novagen
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Aaron Hopkins 02.05.08
|
02.13.08
|-
|
pET-16b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-17b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-19b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-20b(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21a(+)
|
Novagen
|
Matthew Lakich
|
10.2.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21b(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21c(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.25.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-22b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23a(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23b(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23c(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23d(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-24(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
02.13.08
|
02.14.08
|-
|
pET-24a(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24c(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-25b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Katrina Trapp
02.26.07
|
02.27.07
|-
|
pET-26b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-27b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28b(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-28c(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-29b(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29c(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Miriam Garcia
01.30.08
|
01.30.08
|-
|
pET-30a(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30c(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-31b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32a(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32c(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-33b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-39b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-40b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-41a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-42c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-45b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-47b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-48b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-49b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-50b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Pei Yap
02.09.08
|
02.13.08
|-
|
pET-51b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-52b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9a
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9b
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9c
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9d
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pETcoco-1
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pETcoco-2
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLacI
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysE
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysS
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET100/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D/LacZ
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160-GW/CAT
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160/GW/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161-GW/CAT
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161/GW/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pET-DEST42
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST15
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST14
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST17
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pDEST24
|
Invitrogen
|
Matthew Lakich
|
10.04.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pRSET T7
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pRSET-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pCRT7-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
2.29.08
|-
|
pBAD102/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD102/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
Pei 2.19.08
|
02.29.08
|-
|
pBAD-TOPO/LacZ/V5-His
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-DEST49
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
1.23.08
|-
|
pBAD/His B
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/His C
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/myc-His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His B
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His C
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/Myc-His/lacZ
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.17.08
|
01.23.08
|-
|
pBAD/gIII Calmodulin
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/gIII A
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII B
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII C
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.07
|
01.23.08
|-
|
pBAD/Thio-E
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pThioHis A
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
AH 02.28.08
|
03.04.08
|-
|
pThioHis B
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pThioHis C
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO/lacZ
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis2-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis2-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis/CAT
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX-TOPO
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
KT
2.27.08
|
2.29.08
|-
|
pET-30 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-32 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-41 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-43.1 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-44 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-46 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-51 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pCDF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pRSF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBAC-2cp
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBACgus-2cp
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-30 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-32 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-52 3c/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pETDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pACYCDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pCOLADuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.28.07
|
11.27.07
|
11.28.07
|-
|
pCDFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pRSFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pETBlue-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pETBlue-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-3
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pF1A T7
|
Promega
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia
|
11.07.07
|
KT
2.27.08
|
2.29.08
|-
|
pF1K T7
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2A (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2K (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pF3A WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.28
|
02.29.08
|-
|
pF3K WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4A CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4K CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.20.08
|-
|
pF5A CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pF5K CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFN6A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.04.08
|
03.20.08
|-
|
pFN6K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC8A (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFC8K (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF9A CMV hRluc-neo
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN10A (ACT)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN11A (BIND)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF12A RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH
|

|-
|
pF12K RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
|

|-
|
PinPoint Xa-1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
03.02.08
|
03.17.08
|-
|
pALTER-EX1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pALTER-EX2
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pET-5a
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5b
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5c
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-1
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-2
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
PinPoint Control
|
Promega
|
Matthew Lakich
|
10.28.07
|
Miriam Garcia
|
11.05.07
|
1.10.08
|
01.23.08
|-
|
PinPoint Xa Control
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-2
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-3
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Z
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(+)
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(-)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-4Z
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(+)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(+)
|
Promega
|
Grant Richadson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-9Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-11Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-11Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-13Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGL2-Basic
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Control
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Enhancer
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Promoter
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pG5luc
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pSP64 Poly(A)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
3.26.08
|

|-
|
pSP72
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
3.26.08
|

|-
|
pSP73
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
KT
3.26.08
|

|-
|
pGEM-1
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
KT
3.26.08
|

|-
|
pGEM-2
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-3
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-4 Vector
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-15Zf(-)
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
12.13.07
|
01.16.07
|-
|
pSP64
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP65
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP70
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|

|

|-
|
pRS414
|
Simons Lab
|
Miriam Garcia
|
10.25.07
|

|

|
10.26.07
|
11.14.07
|-
|
pRS415
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.13.07
|
11.11.07
|
11.14.07
|-
|
pRS1274
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS591
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS552
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS551
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS577
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS550
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS308
|
Simons Lab
|
Amy Rabb
|
11.12.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS475
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || ||
|}
</protect>

pGEM-1

2008-03-26T18:33:48Z

Trap18: /* Notes */

==At a Glance ==
[[Image:#PGEM-1.jpg|thumb|400px|right|pGEM-1, Image provided by [http://www.promega.com/figures/popup.asp?fn=0978va Promega]]]
[[Image:PGEM1.jpg|thumb|500px|right|pGEM-1, Key: magenta = promoters, red = Ampicillin marker]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://ecoliwiki.net/colipedia/index.php/pGEM-1#Sources Promega]
|-
!{{table heading style}} |Features
|
T7 Promoter 
SP6 Promoter 
multiple cloning sites 
lac operon 
Hind III, Pst I, Sal I, Acc I, Sma I, Hinc II, Xba I, BamH I, Ava I, Sac I, EcoR I
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65300 X65300]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.K471c1a4741bd1&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
The pGEM-1 vector contains SP6/T7 promoters flanking the pUC13 polylinker. It is intended for use as a standard cloning vector as well as for the efficient synthesis of RNA in vitro. The plasmid carries the gene for ampicillin resistance.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources ==
[http://www.promega.com/vectors/pgem1.txt Promega]

==References==
{{RefHelp}}
<references/>

==Sequence ==
<pre>
1 GAATACAAGC TTGGGCTGCA GGTCGACTCT AGAGGATCCC CGGGCGAGCT
51 CGAATTCCGG TCTCCCTATA GTGAGTCGTA TTAATTTCGA TAAGCCAGCT
101 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
151 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
201 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA
251 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
301 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
351 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
401 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
451 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
501 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
551 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
601 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
651 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
701 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
751 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
801 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
851 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
901 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
951 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
1001 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
1051 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
1101 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
1151 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT
1201 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG
1251 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
1301 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
1351 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
1401 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
1451 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT
1501 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
1551 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
1601 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
1651 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
1701 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
1751 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC
1801 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
1851 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
1901 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
1951 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
2001 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
2051 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA
2101 TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT
2151 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT
2201 CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG
2251 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC
2301 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TCGACGCTCT
2351 CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC
2401 GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA
2451 ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG
2501 CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC
2551 GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA
2601 TGCGTCCGGC GTAGAGGATC TGGCTAGCGA TGACCCTGCT GATTGGTTCG
2651 CTGACCATTT CCGGGGTGCG GAACGGCGTT ACCAGAAACT CAGAAGGTTC
2701 GTCCAACCAA ACCGACTCTG ACGGCAGTTT ACGAGAGAGA TGATAGGGTC
2751 TGCTTCAGTA AGCCAGATGC TACACAATTA GGCTTGTACA TATTGTCGTT
2801 AGAACGCGGC TACAATTAAT ACATAACCTT ATGTATCATA CACATACGAT
2851 TTAGGTGACA CTATA
</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]
__NOTOC__

pSP73

2008-03-26T18:25:46Z

Trap18:

==At a Glance ==
[[Image:#PSP73.jpg|thumb|400px|right|pSP73, Image provided by [http://www.promega.com/figures/popup.asp?fn=0300va&partno=P2221&product=pSP73+Vector Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp73.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 transcription initiation site 

T7 transcription initiation site 

SP6 promoter 

T7 promoter 

multiple cloning region 

beta-lactamase coding region 

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6759495 X65333]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R471c1a34a7a07&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
The pSP73 vector can be used as a standard cloning vector and transcription of RNA in vitro. It contains the SP6 and T7 RNA polymerase promoters which flank a unique multiple cloning region. This region includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The two vectors pSP72 and pSP73 are identical except for the orientation of the mutiple cloning site region.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources ==
[http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_222]

==References==
<ref name='PMID:2828872'/>
<references/>

==Sequence ==
<pre>
1 GAACCAGATC TGATATCATC GATGAATTCG AGCTCGGTAC CCGGGGATCC
51 TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT CAGCTGCTCG AGGCCGGTCT
101 CCCTATAGTG AGTCGTATTA ATTTCGATAA GCCAGGTTAA CCTGCATTAA
151 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
201 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
251 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
301 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
351 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
401 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
451 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
501 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
551 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
601 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
651 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
701 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
751 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
801 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
851 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
901 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
951 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
1001 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
1051 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
1101 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
1151 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
1201 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
1251 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
1301 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
1351 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
1401 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
1451 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
1501 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
1551 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
1601 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
1651 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
1701 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
1751 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
1801 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
1851 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
1901 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
1951 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
2001 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
2051 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
2101 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
2151 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG
2201 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
2251 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
2301 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
2351 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGGACAT ATTGTCGTTA
2401 GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACGATT
2451 TAGGTGACAC TATA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:MCS]]
__NOTOC__

pSP73

2008-03-26T18:25:16Z

Trap18: edited by Trap18 via TableEdit

pSP73

2008-03-26T18:23:42Z

Trap18: /* Sources */

==At a Glance ==
[[Image:#PSP73.jpg|thumb|400px|right|pSP73, Image provided by [http://www.promega.com/figures/popup.asp?fn=0300va&partno=P2221&product=pSP73+Vector Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp73.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 promoter 
T7 promoter 
multiple cloning region 
Bgl II, EcoR V, Cla I, EcoR I, Sac I, Kpn I, Sma I, Xba I, BamH I, Sal I, Acc I, Pst I, Sph I, Hind III, Pvu II, Xho
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6759495 X65333]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R471c1a34a7a07&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
The pSP73 vector can be used as a standard cloning vector and transcription of RNA in vitro. It contains the SP6 and T7 RNA polymerase promoters which flank a unique multiple cloning region. This region includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The two vectors pSP72 and pSP73 are identical except for the orientation of the mutiple cloning site region.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources ==
[http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_222]

==References==
<ref name='PMID:2828872'/>
<references/>

==Sequence ==
<pre>
1 GAACCAGATC TGATATCATC GATGAATTCG AGCTCGGTAC CCGGGGATCC
51 TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT CAGCTGCTCG AGGCCGGTCT
101 CCCTATAGTG AGTCGTATTA ATTTCGATAA GCCAGGTTAA CCTGCATTAA
151 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
201 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
251 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
301 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
351 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
401 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
451 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
501 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
551 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
601 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
651 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
701 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
751 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
801 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
851 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
901 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
951 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
1001 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
1051 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
1101 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
1151 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
1201 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
1251 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
1301 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
1351 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
1401 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
1451 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
1501 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
1551 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
1601 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
1651 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
1701 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
1751 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
1801 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
1851 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
1901 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
1951 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
2001 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
2051 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
2101 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
2151 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG
2201 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
2251 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
2301 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
2351 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGGACAT ATTGTCGTTA
2401 GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACGATT
2451 TAGGTGACAC TATA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]
__NOTOC__

pSP73

2008-03-26T18:23:02Z

Trap18: /* Notes */

==At a Glance ==
[[Image:#PSP73.jpg|thumb|400px|right|pSP73, Image provided by [http://www.promega.com/figures/popup.asp?fn=0300va&partno=P2221&product=pSP73+Vector Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp73.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 promoter 
T7 promoter 
multiple cloning region 
Bgl II, EcoR V, Cla I, EcoR I, Sac I, Kpn I, Sma I, Xba I, BamH I, Sal I, Acc I, Pst I, Sph I, Hind III, Pvu II, Xho
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6759495 X65333]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R471c1a34a7a07&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
The pSP73 vector can be used as a standard cloning vector and transcription of RNA in vitro. It contains the SP6 and T7 RNA polymerase promoters which flank a unique multiple cloning region. This region includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The two vectors pSP72 and pSP73 are identical except for the orientation of the mutiple cloning site region.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources ==
[http://www.promega.com/vectors/psp73.txt Promega]

==References==
<ref name='PMID:2828872'/>
<references/>

==Sequence ==
<pre>
1 GAACCAGATC TGATATCATC GATGAATTCG AGCTCGGTAC CCGGGGATCC
51 TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT CAGCTGCTCG AGGCCGGTCT
101 CCCTATAGTG AGTCGTATTA ATTTCGATAA GCCAGGTTAA CCTGCATTAA
151 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
201 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
251 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
301 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
351 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
401 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
451 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
501 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
551 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
601 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
651 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
701 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
751 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
801 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
851 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
901 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
951 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
1001 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
1051 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
1101 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
1151 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
1201 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
1251 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
1301 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
1351 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
1401 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
1451 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
1501 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
1551 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
1601 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
1651 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
1701 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
1751 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
1801 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
1851 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
1901 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
1951 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
2001 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
2051 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
2101 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
2151 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG
2201 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
2251 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
2301 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
2351 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGGACAT ATTGTCGTTA
2401 GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACGATT
2451 TAGGTGACAC TATA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]
__NOTOC__

EcoliWiki:Tracking Plasmids

2008-03-26T18:21:34Z

Trap18: edited by Trap18 via TableEdit

This is a page to track plasmids entered into the wiki. It is for developmental purposes only.

<protect>

{|class = 'sortable' {{Prettytable}} id='220572'
|-
!Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed!!Date Info Added!!Date Reviewed
|-
|
LITMUS 28i
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.03.07
|
11.12.07
|
11.13.07
|-
|
LITMUS 38i
|
NEB
|
Miriam Garcia
|
08.31.2007
|
Miriam Garcia
|
08.31.07
|
10.31.07
|
10.31.07
|-
|
pGPS1.1
|
NEB
|
Charner Williams
|
08.31.07
|
Miriam Garcia
|
09.04.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pGPS2.1
|
NEB
|
Matthew Lakich
|
09.04.07
|
Miriam Garcia
|
09.06.07
|
12.04.07
|
12.17.07
|-
|
pGPS3
|
NEB
|
Grant Richardson
|
09.07.07
|
Miriam Garcia
|
09.10.07
|
Aaron Hopkins
01.22.08
|
01.23.08
|-
|
pGPS4
|
NEB
|
Amy Rabb
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
Joseph Kim
01.22.08
|
01.23.08
|-
|
pGPS5
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
11.12.07
|
11.13.07
|-
|
pMAL-c4E
|
NEB
|
Amy Rabb
|
09.10.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.21.08
|
01.23.08
|-
|
pMAL-c4G
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.31.08
|
11.13.07
|-
|
pMAL-c4X
|
NEB
|
Matthew Lakich
|
9.13.07
|
Miriam Garcia
|
09.14.07
|
12.17.07 Miriam Garcia
|
12.17.07
|-
|
pMAL-p4E
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.07.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4G
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4X
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-pIII
|
NEB
|
Charner Williams
|
09.08.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pNEB193
|
NEB
|
CharnerWilliams
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pNEB206A
|
NEB
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 09.19.07
|
09.19.07
|-
|
pTWIN1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB3
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.12.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB11
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pUC19
|
NEB
|

|

|
Miriam Garcia
|
09.26.07
|
Aaron Hopkins
|

|-
|
pNEBR-R1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1GLuc
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1Hygro
|
NEB
|
Grant Richardson
|
09.10.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1-malE
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pCMV-GLuc
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pGLuc-Basic Vector
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-Q
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-Q
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-SET1
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET1-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SET1
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SET2
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEc-SET3
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1a
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1b
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.30.08
|-
|
pBEn-SBP-SET1c
|
Stratagene
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBPa
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPb
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPc
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pBEn-SET1a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.24.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-kc
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n-EK
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pCAL-n-FLAG
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pET-11a
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11b
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
02.05.08
|
02.07.08
|-
|
pET-11c
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11d
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3a
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.04.08
|
02.13.08
|-
|
pET-3b
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3c
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3d
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET100/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
09.10.07
|

|

|
11.01.07
|

|-
|
pET-14b
|
Novagen
|
Miriam Garcia
|
09.19.07
|

|

|
11.07.07
|

|-
|
pET-15b
|
Novagen
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Aaron Hopkins 02.05.08
|
02.13.08
|-
|
pET-16b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-17b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-19b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-20b(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21a(+)
|
Novagen
|
Matthew Lakich
|
10.2.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21b(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21c(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.25.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-22b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23a(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23b(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23c(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23d(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-24(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
02.13.08
|
02.14.08
|-
|
pET-24a(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24c(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-25b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Katrina Trapp
02.26.07
|
02.27.07
|-
|
pET-26b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-27b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28b(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-28c(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-29b(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29c(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Miriam Garcia
01.30.08
|
01.30.08
|-
|
pET-30a(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30c(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-31b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32a(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32c(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-33b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-39b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-40b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-41a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-42c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-45b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-47b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-48b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-49b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-50b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Pei Yap
02.09.08
|
02.13.08
|-
|
pET-51b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-52b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9a
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9b
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9c
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9d
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pETcoco-1
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pETcoco-2
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLacI
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysE
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysS
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET100/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D/LacZ
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160-GW/CAT
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160/GW/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161-GW/CAT
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161/GW/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pET-DEST42
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST15
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST14
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST17
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pDEST24
|
Invitrogen
|
Matthew Lakich
|
10.04.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pRSET T7
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pRSET-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pCRT7-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
2.29.08
|-
|
pBAD102/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD102/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
Pei 2.19.08
|
02.29.08
|-
|
pBAD-TOPO/LacZ/V5-His
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-DEST49
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
1.23.08
|-
|
pBAD/His B
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/His C
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/myc-His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His B
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His C
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/Myc-His/lacZ
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.17.08
|
01.23.08
|-
|
pBAD/gIII Calmodulin
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/gIII A
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII B
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII C
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.07
|
01.23.08
|-
|
pBAD/Thio-E
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pThioHis A
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
AH 02.28.08
|
03.04.08
|-
|
pThioHis B
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pThioHis C
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO/lacZ
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis2-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis2-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis/CAT
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX-TOPO
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
KT
2.27.08
|
2.29.08
|-
|
pET-30 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-32 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-41 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-43.1 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-44 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-46 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-51 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pCDF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pRSF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBAC-2cp
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBACgus-2cp
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-30 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-32 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-52 3c/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pETDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pACYCDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pCOLADuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.28.07
|
11.27.07
|
11.28.07
|-
|
pCDFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pRSFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pETBlue-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pETBlue-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-3
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pF1A T7
|
Promega
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia
|
11.07.07
|
KT
2.27.08
|
2.29.08
|-
|
pF1K T7
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2A (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2K (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pF3A WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.28
|
02.29.08
|-
|
pF3K WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4A CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4K CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.20.08
|-
|
pF5A CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pF5K CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFN6A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.04.08
|
03.20.08
|-
|
pFN6K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC8A (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFC8K (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF9A CMV hRluc-neo
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN10A (ACT)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN11A (BIND)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF12A RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH
|

|-
|
pF12K RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
|

|-
|
PinPoint Xa-1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
03.02.08
|
03.17.08
|-
|
pALTER-EX1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pALTER-EX2
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pET-5a
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5b
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5c
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-1
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-2
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
PinPoint Control
|
Promega
|
Matthew Lakich
|
10.28.07
|
Miriam Garcia
|
11.05.07
|
1.10.08
|
01.23.08
|-
|
PinPoint Xa Control
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-2
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-3
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Z
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(+)
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(-)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-4Z
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(+)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(+)
|
Promega
|
Grant Richadson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-9Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-11Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-11Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-13Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGL2-Basic
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Control
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Enhancer
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Promoter
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pG5luc
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pSP64 Poly(A)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
3.26.08
|

|-
|
pSP72
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
3.26.08
|

|-
|
pSP73
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
KT
|

|-
|
pGEM-1
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
KT
|

|-
|
pGEM-2
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-3
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-4 Vector
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-15Zf(-)
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
12.13.07
|
01.16.07
|-
|
pSP64
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP65
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP70
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|

|

|-
|
pRS414
|
Simons Lab
|
Miriam Garcia
|
10.25.07
|

|

|
10.26.07
|
11.14.07
|-
|
pRS415
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.13.07
|
11.11.07
|
11.14.07
|-
|
pRS1274
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS591
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS552
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS551
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS577
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS550
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS308
|
Simons Lab
|
Amy Rabb
|
11.12.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS475
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || ||
|}
</protect>

pSP72

2008-03-26T18:20:57Z

Trap18: /* Sources */

==At a Glance==
[[Image:#pSP72.gif|thumb|right|300px|pSP72, image provided by [http://www.promega.com/figures/popup.asp?fn=0298va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp72.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 transcription initiation site 

T7 transcription initiation site 

SP6 promoter 

T7 promoter 

multiple cloning region 

beta-lactamase coding region 

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6759494 X65332]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.W471e0a3be34a8&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pSP72 vector can be used as a standard cloning vector and transcription of RNA in vitro. It contains the SP6 and T7 RNA polymerase promoters which flank a unique multiple cloning region. This region includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The two vectors pSP72 and pSP73 are identical except for the orientation of the mutiple cloning site region.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_221]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GAACTCGAGC AGCTGAAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC

51 CCCGGGTACC GAGCTCGAAT TCATCGATGA TATCAGATCT GCCGGTCTCC

101 CTATAGTGAG TCGTATTAAT TTCGATAAGC CAGGTTAACC TGCATTAATG

151 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG

201 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG

251 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA

301 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC

351 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC

401 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG

451 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC

501 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG

551 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT

601 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC

651 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA

701 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG

751 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT

801 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA

851 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC

901 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA

951 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT

1001 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA

1051 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA

1101 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC

1151 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC

1201 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG

1251 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT

1301 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC

1351 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA

1401 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT

1451 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC

1501 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA

1551 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC

1601 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT

1651 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT

1701 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA

1751 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT

1801 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA

1851 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT

1901 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC

1951 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT

2001 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC

2051 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG

2101 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA

2151 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG

2201 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC

2251 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG

2301 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA

2351 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGGACATAT TGTCGTTAGA

2401 ACGCGGCTAC AATTAATACA TAACCTTATG TATCATACAC ATACGATTTA

2451 GGTGACACTA TA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]
__NOTOC__

pSP72

2008-03-26T18:20:31Z

Trap18:

==At a Glance==
[[Image:#pSP72.gif|thumb|right|300px|pSP72, image provided by [http://www.promega.com/figures/popup.asp?fn=0298va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp72.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 transcription initiation site 

T7 transcription initiation site 

SP6 promoter 

T7 promoter 

multiple cloning region 

beta-lactamase coding region 

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6759494 X65332]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.W471e0a3be34a8&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pSP72 vector can be used as a standard cloning vector and transcription of RNA in vitro. It contains the SP6 and T7 RNA polymerase promoters which flank a unique multiple cloning region. This region includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The two vectors pSP72 and pSP73 are identical except for the orientation of the mutiple cloning site region.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pSP72+Vector&description%5Ftext=pSP72+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GAACTCGAGC AGCTGAAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC

51 CCCGGGTACC GAGCTCGAAT TCATCGATGA TATCAGATCT GCCGGTCTCC

101 CTATAGTGAG TCGTATTAAT TTCGATAAGC CAGGTTAACC TGCATTAATG

151 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG

201 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG

251 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA

301 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC

351 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC

401 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG

451 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC

501 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG

551 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT

601 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC

651 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA

701 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG

751 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT

801 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA

851 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC

901 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA

951 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT

1001 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA

1051 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA

1101 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC

1151 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC

1201 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG

1251 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT

1301 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC

1351 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA

1401 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT

1451 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC

1501 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA

1551 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC

1601 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT

1651 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT

1701 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA

1751 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT

1801 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA

1851 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT

1901 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC

1951 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT

2001 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC

2051 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG

2101 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA

2151 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG

2201 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC

2251 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG

2301 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA

2351 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGGACATAT TGTCGTTAGA

2401 ACGCGGCTAC AATTAATACA TAACCTTATG TATCATACAC ATACGATTTA

2451 GGTGACACTA TA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]
__NOTOC__

pSP72

2008-03-26T18:19:34Z

Trap18: edited by Trap18 via TableEdit

==At a Glance==
[[Image:#pSP72.gif|thumb|right|300px|pSP72, image provided by [http://www.promega.com/figures/popup.asp?fn=0298va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp72.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 transcription initiation site 

T7 transcription initiation site 

SP6 promoter 

T7 promoter 

multiple cloning region 

beta-lactamase coding region 

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6759494 X65332]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.W471e0a3be34a8&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pSP72 vector can be used as a standard cloning vector and transcription of RNA in vitro. It contains the SP6 and T7 RNA polymerase promoters which flank a unique multiple cloning region. This region includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The two vectors pSP72 and pSP73 are identical except for the orientation of the mutiple cloning site region.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pSP72+Vector&description%5Ftext=pSP72+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GAACTCGAGC AGCTGAAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC

51 CCCGGGTACC GAGCTCGAAT TCATCGATGA TATCAGATCT GCCGGTCTCC

101 CTATAGTGAG TCGTATTAAT TTCGATAAGC CAGGTTAACC TGCATTAATG

151 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG

201 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG

251 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA

301 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC

351 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC

401 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG

451 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC

501 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG

551 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT

601 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC

651 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA

701 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG

751 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT

801 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA

851 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC

901 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA

951 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT

1001 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA

1051 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA

1101 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC

1151 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC

1201 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG

1251 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT

1301 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC

1351 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA

1401 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT

1451 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC

1501 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA

1551 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC

1601 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT

1651 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT

1701 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA

1751 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT

1801 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA

1851 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT

1901 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC

1951 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT

2001 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC

2051 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG

2101 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA

2151 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG

2201 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC

2251 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG

2301 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA

2351 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGGACATAT TGTCGTTAGA

2401 ACGCGGCTAC AATTAATACA TAACCTTATG TATCATACAC ATACGATTTA

2451 GGTGACACTA TA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pSP72

2008-03-26T18:17:38Z

Trap18: /* Notes */

==At a Glance==
[[Image:#pSP72.gif|thumb|right|300px|pSP72, image provided by [http://www.promega.com/figures/popup.asp?fn=0298va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp72.txt Promega]
|-
!{{table heading style}} |Features
|

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6759494 X65332]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.W471e0a3be34a8&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pSP72 vector can be used as a standard cloning vector and transcription of RNA in vitro. It contains the SP6 and T7 RNA polymerase promoters which flank a unique multiple cloning region. This region includes restriction sites for XhoI, PvuII, HindIII, SphI, PstI, SalI, AccI, XbaI, BamHI, SmaI, KpnI, SacI, EcoRI, ClaI, EcoRV and BglII. The two vectors pSP72 and pSP73 are identical except for the orientation of the mutiple cloning site region.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pSP72+Vector&description%5Ftext=pSP72+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GAACTCGAGC AGCTGAAGCT TGCATGCCTG CAGGTCGACT CTAGAGGATC

51 CCCGGGTACC GAGCTCGAAT TCATCGATGA TATCAGATCT GCCGGTCTCC

101 CTATAGTGAG TCGTATTAAT TTCGATAAGC CAGGTTAACC TGCATTAATG

151 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG

201 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG

251 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA

301 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC

351 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC

401 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG

451 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC

501 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG

551 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT

601 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC

651 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA

701 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG

751 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT

801 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA

851 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC

901 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA

951 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT

1001 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA

1051 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA

1101 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC

1151 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC

1201 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG

1251 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT

1301 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC

1351 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA

1401 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT

1451 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC

1501 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA

1551 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC

1601 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT

1651 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT

1701 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA

1751 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT

1801 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA

1851 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT

1901 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC

1951 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT

2001 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC

2051 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG

2101 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA

2151 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG

2201 CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC

2251 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG

2301 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA

2351 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGGACATAT TGTCGTTAGA

2401 ACGCGGCTAC AATTAATACA TAACCTTATG TATCATACAC ATACGATTTA

2451 GGTGACACTA TA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

EcoliWiki:Tracking Plasmids

2008-03-26T18:12:54Z

Trap18: edited by Trap18 via TableEdit

This is a page to track plasmids entered into the wiki. It is for developmental purposes only.

<protect>

{|class = 'sortable' {{Prettytable}} id='220572'
|-
!Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed!!Date Info Added!!Date Reviewed
|-
|
LITMUS 28i
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.03.07
|
11.12.07
|
11.13.07
|-
|
LITMUS 38i
|
NEB
|
Miriam Garcia
|
08.31.2007
|
Miriam Garcia
|
08.31.07
|
10.31.07
|
10.31.07
|-
|
pGPS1.1
|
NEB
|
Charner Williams
|
08.31.07
|
Miriam Garcia
|
09.04.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pGPS2.1
|
NEB
|
Matthew Lakich
|
09.04.07
|
Miriam Garcia
|
09.06.07
|
12.04.07
|
12.17.07
|-
|
pGPS3
|
NEB
|
Grant Richardson
|
09.07.07
|
Miriam Garcia
|
09.10.07
|
Aaron Hopkins
01.22.08
|
01.23.08
|-
|
pGPS4
|
NEB
|
Amy Rabb
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
Joseph Kim
01.22.08
|
01.23.08
|-
|
pGPS5
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
11.12.07
|
11.13.07
|-
|
pMAL-c4E
|
NEB
|
Amy Rabb
|
09.10.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.21.08
|
01.23.08
|-
|
pMAL-c4G
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.31.08
|
11.13.07
|-
|
pMAL-c4X
|
NEB
|
Matthew Lakich
|
9.13.07
|
Miriam Garcia
|
09.14.07
|
12.17.07 Miriam Garcia
|
12.17.07
|-
|
pMAL-p4E
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.07.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4G
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4X
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-pIII
|
NEB
|
Charner Williams
|
09.08.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pNEB193
|
NEB
|
CharnerWilliams
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pNEB206A
|
NEB
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 09.19.07
|
09.19.07
|-
|
pTWIN1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB3
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.12.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB11
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pUC19
|
NEB
|

|

|
Miriam Garcia
|
09.26.07
|
Aaron Hopkins
|

|-
|
pNEBR-R1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1GLuc
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1Hygro
|
NEB
|
Grant Richardson
|
09.10.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1-malE
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pCMV-GLuc
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pGLuc-Basic Vector
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-Q
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-Q
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-SET1
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET1-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SET1
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SET2
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEc-SET3
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1a
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1b
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.30.08
|-
|
pBEn-SBP-SET1c
|
Stratagene
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBPa
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPb
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPc
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pBEn-SET1a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.24.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-kc
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n-EK
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pCAL-n-FLAG
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pET-11a
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11b
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
02.05.08
|
02.07.08
|-
|
pET-11c
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11d
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3a
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.04.08
|
02.13.08
|-
|
pET-3b
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3c
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3d
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET100/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
09.10.07
|

|

|
11.01.07
|

|-
|
pET-14b
|
Novagen
|
Miriam Garcia
|
09.19.07
|

|

|
11.07.07
|

|-
|
pET-15b
|
Novagen
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Aaron Hopkins 02.05.08
|
02.13.08
|-
|
pET-16b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-17b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-19b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-20b(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21a(+)
|
Novagen
|
Matthew Lakich
|
10.2.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21b(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21c(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.25.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-22b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23a(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23b(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23c(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23d(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-24(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
02.13.08
|
02.14.08
|-
|
pET-24a(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24c(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-25b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Katrina Trapp
02.26.07
|
02.27.07
|-
|
pET-26b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-27b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28b(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-28c(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-29b(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29c(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Miriam Garcia
01.30.08
|
01.30.08
|-
|
pET-30a(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30c(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-31b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32a(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32c(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-33b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-39b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-40b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-41a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-42c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-45b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-47b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-48b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-49b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-50b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Pei Yap
02.09.08
|
02.13.08
|-
|
pET-51b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-52b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9a
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9b
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9c
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9d
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pETcoco-1
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pETcoco-2
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLacI
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysE
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysS
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET100/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D/LacZ
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160-GW/CAT
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160/GW/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161-GW/CAT
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161/GW/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pET-DEST42
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST15
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST14
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST17
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pDEST24
|
Invitrogen
|
Matthew Lakich
|
10.04.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pRSET T7
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pRSET-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pCRT7-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
2.29.08
|-
|
pBAD102/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD102/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
Pei 2.19.08
|
02.29.08
|-
|
pBAD-TOPO/LacZ/V5-His
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-DEST49
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
1.23.08
|-
|
pBAD/His B
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/His C
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/myc-His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His B
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His C
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/Myc-His/lacZ
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.17.08
|
01.23.08
|-
|
pBAD/gIII Calmodulin
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/gIII A
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII B
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII C
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.07
|
01.23.08
|-
|
pBAD/Thio-E
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pThioHis A
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
AH 02.28.08
|
03.04.08
|-
|
pThioHis B
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pThioHis C
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO/lacZ
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis2-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis2-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis/CAT
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX-TOPO
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
KT
2.27.08
|
2.29.08
|-
|
pET-30 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-32 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-41 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-43.1 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-44 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-46 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-51 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pCDF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pRSF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBAC-2cp
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBACgus-2cp
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-30 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-32 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-52 3c/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pETDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pACYCDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pCOLADuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.28.07
|
11.27.07
|
11.28.07
|-
|
pCDFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pRSFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pETBlue-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pETBlue-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-3
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pF1A T7
|
Promega
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia
|
11.07.07
|
KT
2.27.08
|
2.29.08
|-
|
pF1K T7
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2A (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2K (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pF3A WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.28
|
02.29.08
|-
|
pF3K WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4A CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4K CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.20.08
|-
|
pF5A CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pF5K CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFN6A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.04.08
|
03.20.08
|-
|
pFN6K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC8A (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFC8K (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF9A CMV hRluc-neo
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN10A (ACT)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN11A (BIND)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF12A RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH
|

|-
|
pF12K RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
|

|-
|
PinPoint Xa-1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
03.02.08
|
03.17.08
|-
|
pALTER-EX1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pALTER-EX2
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pET-5a
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5b
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5c
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-1
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-2
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
PinPoint Control
|
Promega
|
Matthew Lakich
|
10.28.07
|
Miriam Garcia
|
11.05.07
|
1.10.08
|
01.23.08
|-
|
PinPoint Xa Control
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-2
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-3
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Z
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(+)
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(-)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-4Z
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(+)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(+)
|
Promega
|
Grant Richadson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-9Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-11Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-11Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-13Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGL2-Basic
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Control
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Enhancer
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Promoter
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pG5luc
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pSP64 Poly(A)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
3.26.08
|

|-
|
pSP72
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
|

|-
|
pSP73
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
KT
|

|-
|
pGEM-1
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
KT
|

|-
|
pGEM-2
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-3
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-4 Vector
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-15Zf(-)
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
12.13.07
|
01.16.07
|-
|
pSP64
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP65
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP70
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|

|

|-
|
pRS414
|
Simons Lab
|
Miriam Garcia
|
10.25.07
|

|

|
10.26.07
|
11.14.07
|-
|
pRS415
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.13.07
|
11.11.07
|
11.14.07
|-
|
pRS1274
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS591
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS552
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS551
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS577
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS550
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS308
|
Simons Lab
|
Amy Rabb
|
11.12.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS475
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || ||
|}
</protect>

pSP64 Poly(A)

2008-03-26T18:12:02Z

Trap18: edited by Trap18 via TableEdit

==At a Glance==
[[Image:#pSP64 Poly(A).gif|thumb|right|300px|pSP64 Poly(A), image provided by [http://www.promega.com/figures/popup.asp?fn=0306va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp64pa.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 RNA polymerase transcription initiation site 

SP6 RNA polymerase promoter 

multiple cloningregion 

Poly(A)region 

beta-lactamase (Ampr) codingregion 

binding site of pUC/M13 Reverse Primer 
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7688144 X65328]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B471e0a1806df3&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pSP64 Poly(A) Vector is able to be used as a standaed cloning vector and also for in vitro transcription from the SP6 promoter. The unique feature of the pSP64 Poly (A) Vector is the fact that is can also be used to generate poly(A)+ transcripts in vitro. The vector contains a stretch of 30 dA:dT redsidues which are inserted between SacI and EcorRI sites. WHen foreign DNA is cloned into any polylinker site other than EcoRI in the pSP64 vector causes linearization of the recombinant plasmid with EcoRI occurs. This allows the use of SP6 RNA polymerase in vitro to prepare RNA copies. These copies are of the inserted sequences that conatin a synthetic 3' "poly (A)" tail of 30 residues.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_225]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GAATACAAGC TTGGGCTGCA GGTCGACTCT AGAGGATCCC CGGGCGAGCT

51 CCCAAAAAAA AAAAAAAAAA AAAAAAAAAA AAACCGAATT CGTAATCATG

101 TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA

151 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA

201 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA

251 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG

301 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC

351 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA

401 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC

451 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT

501 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA

551 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC

601 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG

651 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC

701 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG

751 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA

801 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA

851 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC

901 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT

951 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT

1001 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT

1051 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT

1101 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA

1151 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT

1201 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT

1251 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC

1301 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC

1351 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC

1401 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC

1451 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA

1501 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA

1551 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG

1601 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT

1651 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC

1701 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG

1751 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC

1801 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC

1851 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT

1901 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA

1951 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC

2001 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG

2051 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC

2101 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA

2151 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG

2201 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC

2251 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC

2301 GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC

2351 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA

2401 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT

2451 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG

2501 CACCATTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA

2551 GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC

2601 AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC

2651 CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC

2701 CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG

2751 GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCTGGC TAGCGATGAC

2801 CCTGCTGATT GGTTCGCTGA CCATTTCCGG GTGCGGGACG GCGTTACCAG

2851 AAACTCAGAA GGTTCGTCCA ACCAAACCGA CTCTGACGGC AGTTTACGAG

2901 AGAGATGATA GGGTCTGCTT CAGTAAGCCA GATGCTACAC AATTAGGCTT

2951 GTACATATTG TCGTTAGAAC GCGGCTACAA TTAATACATA ACCTTATGTA

3001 TCATACACAT ACGATTTAGG TGACACTATA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pSP64 Poly(A)

2008-03-26T18:09:32Z

Trap18: /* Sources */

==At a Glance==
[[Image:#pSP64 Poly(A).gif|thumb|right|300px|pSP64 Poly(A), image provided by [http://www.promega.com/figures/popup.asp?fn=0306va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp64pa.txt Promega]
|-
!{{table heading style}} |Features
|

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7688144 X65328]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B471e0a1806df3&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pSP64 Poly(A) Vector is able to be used as a standaed cloning vector and also for in vitro transcription from the SP6 promoter. The unique feature of the pSP64 Poly (A) Vector is the fact that is can also be used to generate poly(A)+ transcripts in vitro. The vector contains a stretch of 30 dA:dT redsidues which are inserted between SacI and EcorRI sites. WHen foreign DNA is cloned into any polylinker site other than EcoRI in the pSP64 vector causes linearization of the recombinant plasmid with EcoRI occurs. This allows the use of SP6 RNA polymerase in vitro to prepare RNA copies. These copies are of the inserted sequences that conatin a synthetic 3' "poly (A)" tail of 30 residues.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_225]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GAATACAAGC TTGGGCTGCA GGTCGACTCT AGAGGATCCC CGGGCGAGCT

51 CCCAAAAAAA AAAAAAAAAA AAAAAAAAAA AAACCGAATT CGTAATCATG

101 TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA

151 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA

201 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA

251 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG

301 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC

351 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA

401 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC

451 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT

501 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA

551 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC

601 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG

651 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC

701 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG

751 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA

801 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA

851 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC

901 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT

951 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT

1001 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT

1051 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT

1101 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA

1151 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT

1201 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT

1251 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC

1301 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC

1351 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC

1401 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC

1451 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA

1501 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA

1551 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG

1601 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT

1651 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC

1701 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG

1751 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC

1801 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC

1851 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT

1901 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA

1951 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC

2001 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG

2051 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC

2101 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA

2151 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG

2201 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC

2251 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC

2301 GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC

2351 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA

2401 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT

2451 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG

2501 CACCATTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA

2551 GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC

2601 AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC

2651 CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC

2701 CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG

2751 GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCTGGC TAGCGATGAC

2801 CCTGCTGATT GGTTCGCTGA CCATTTCCGG GTGCGGGACG GCGTTACCAG

2851 AAACTCAGAA GGTTCGTCCA ACCAAACCGA CTCTGACGGC AGTTTACGAG

2901 AGAGATGATA GGGTCTGCTT CAGTAAGCCA GATGCTACAC AATTAGGCTT

2951 GTACATATTG TCGTTAGAAC GCGGCTACAA TTAATACATA ACCTTATGTA

3001 TCATACACAT ACGATTTAGG TGACACTATA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pSP64 Poly(A)

2008-03-26T18:09:12Z

Trap18: /* Notes */

==At a Glance==
[[Image:#pSP64 Poly(A).gif|thumb|right|300px|pSP64 Poly(A), image provided by [http://www.promega.com/figures/popup.asp?fn=0306va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/psp64pa.txt Promega]
|-
!{{table heading style}} |Features
|

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7688144 X65328]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B471e0a1806df3&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pSP64 Poly(A) Vector is able to be used as a standaed cloning vector and also for in vitro transcription from the SP6 promoter. The unique feature of the pSP64 Poly (A) Vector is the fact that is can also be used to generate poly(A)+ transcripts in vitro. The vector contains a stretch of 30 dA:dT redsidues which are inserted between SacI and EcorRI sites. WHen foreign DNA is cloned into any polylinker site other than EcoRI in the pSP64 vector causes linearization of the recombinant plasmid with EcoRI occurs. This allows the use of SP6 RNA polymerase in vitro to prepare RNA copies. These copies are of the inserted sequences that conatin a synthetic 3' "poly (A)" tail of 30 residues.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pSP64+Poly%28A%29+Vector&description%5Ftext=pSP64+Poly%28A%29+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GAATACAAGC TTGGGCTGCA GGTCGACTCT AGAGGATCCC CGGGCGAGCT

51 CCCAAAAAAA AAAAAAAAAA AAAAAAAAAA AAACCGAATT CGTAATCATG

101 TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA

151 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA

201 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA

251 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG

301 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC

351 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA

401 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC

451 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT

501 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA

551 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC

601 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG

651 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC

701 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG

751 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA

801 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA

851 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC

901 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT

951 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT

1001 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT

1051 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT

1101 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA

1151 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT

1201 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT

1251 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC

1301 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC

1351 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC

1401 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC

1451 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA

1501 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA

1551 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG

1601 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT

1651 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC

1701 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG

1751 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC

1801 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC

1851 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT

1901 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA

1951 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC

2001 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG

2051 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC

2101 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA

2151 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG

2201 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC

2251 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC

2301 GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC

2351 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA

2401 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT

2451 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG

2501 CACCATTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA

2551 GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC

2601 AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC

2651 CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC

2701 CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG

2751 GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCTGGC TAGCGATGAC

2801 CCTGCTGATT GGTTCGCTGA CCATTTCCGG GTGCGGGACG GCGTTACCAG

2851 AAACTCAGAA GGTTCGTCCA ACCAAACCGA CTCTGACGGC AGTTTACGAG

2901 AGAGATGATA GGGTCTGCTT CAGTAAGCCA GATGCTACAC AATTAGGCTT

2951 GTACATATTG TCGTTAGAAC GCGGCTACAA TTAATACATA ACCTTATGTA

3001 TCATACACAT ACGATTTAGG TGACACTATA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

EcoliWiki:Tracking Plasmids

2008-03-26T18:01:58Z

Trap18: edited by Trap18 via TableEdit

This is a page to track plasmids entered into the wiki. It is for developmental purposes only.

<protect>

{|class = 'sortable' {{Prettytable}} id='220572'
|-
!Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed!!Date Info Added!!Date Reviewed
|-
|
LITMUS 28i
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.03.07
|
11.12.07
|
11.13.07
|-
|
LITMUS 38i
|
NEB
|
Miriam Garcia
|
08.31.2007
|
Miriam Garcia
|
08.31.07
|
10.31.07
|
10.31.07
|-
|
pGPS1.1
|
NEB
|
Charner Williams
|
08.31.07
|
Miriam Garcia
|
09.04.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pGPS2.1
|
NEB
|
Matthew Lakich
|
09.04.07
|
Miriam Garcia
|
09.06.07
|
12.04.07
|
12.17.07
|-
|
pGPS3
|
NEB
|
Grant Richardson
|
09.07.07
|
Miriam Garcia
|
09.10.07
|
Aaron Hopkins
01.22.08
|
01.23.08
|-
|
pGPS4
|
NEB
|
Amy Rabb
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
Joseph Kim
01.22.08
|
01.23.08
|-
|
pGPS5
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
11.12.07
|
11.13.07
|-
|
pMAL-c4E
|
NEB
|
Amy Rabb
|
09.10.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.21.08
|
01.23.08
|-
|
pMAL-c4G
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.31.08
|
11.13.07
|-
|
pMAL-c4X
|
NEB
|
Matthew Lakich
|
9.13.07
|
Miriam Garcia
|
09.14.07
|
12.17.07 Miriam Garcia
|
12.17.07
|-
|
pMAL-p4E
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.07.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4G
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4X
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-pIII
|
NEB
|
Charner Williams
|
09.08.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pNEB193
|
NEB
|
CharnerWilliams
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pNEB206A
|
NEB
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 09.19.07
|
09.19.07
|-
|
pTWIN1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB3
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.12.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB11
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pUC19
|
NEB
|

|

|
Miriam Garcia
|
09.26.07
|
Aaron Hopkins
|

|-
|
pNEBR-R1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1GLuc
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1Hygro
|
NEB
|
Grant Richardson
|
09.10.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1-malE
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pCMV-GLuc
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pGLuc-Basic Vector
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-Q
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-Q
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-SET1
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET1-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SET1
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SET2
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEc-SET3
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1a
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1b
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.30.08
|-
|
pBEn-SBP-SET1c
|
Stratagene
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBPa
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPb
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPc
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pBEn-SET1a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.24.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-kc
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n-EK
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pCAL-n-FLAG
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pET-11a
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11b
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
02.05.08
|
02.07.08
|-
|
pET-11c
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11d
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3a
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.04.08
|
02.13.08
|-
|
pET-3b
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3c
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3d
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET100/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
09.10.07
|

|

|
11.01.07
|

|-
|
pET-14b
|
Novagen
|
Miriam Garcia
|
09.19.07
|

|

|
11.07.07
|

|-
|
pET-15b
|
Novagen
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Aaron Hopkins 02.05.08
|
02.13.08
|-
|
pET-16b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-17b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-19b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-20b(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21a(+)
|
Novagen
|
Matthew Lakich
|
10.2.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21b(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21c(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.25.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-22b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23a(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23b(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23c(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23d(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-24(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
02.13.08
|
02.14.08
|-
|
pET-24a(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24c(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-25b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Katrina Trapp
02.26.07
|
02.27.07
|-
|
pET-26b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-27b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28b(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-28c(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-29b(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29c(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Miriam Garcia
01.30.08
|
01.30.08
|-
|
pET-30a(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30c(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-31b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32a(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32c(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-33b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-39b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-40b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-41a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-42c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-45b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-47b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-48b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-49b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-50b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Pei Yap
02.09.08
|
02.13.08
|-
|
pET-51b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-52b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9a
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9b
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9c
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9d
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pETcoco-1
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pETcoco-2
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLacI
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysE
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysS
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET100/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D/LacZ
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160-GW/CAT
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160/GW/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161-GW/CAT
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161/GW/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pET-DEST42
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST15
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST14
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST17
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pDEST24
|
Invitrogen
|
Matthew Lakich
|
10.04.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pRSET T7
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pRSET-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pCRT7-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
2.29.08
|-
|
pBAD102/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD102/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
Pei 2.19.08
|
02.29.08
|-
|
pBAD-TOPO/LacZ/V5-His
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-DEST49
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
1.23.08
|-
|
pBAD/His B
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/His C
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/myc-His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His B
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His C
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/Myc-His/lacZ
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.17.08
|
01.23.08
|-
|
pBAD/gIII Calmodulin
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/gIII A
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII B
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII C
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.07
|
01.23.08
|-
|
pBAD/Thio-E
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pThioHis A
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
AH 02.28.08
|
03.04.08
|-
|
pThioHis B
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pThioHis C
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO/lacZ
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis2-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis2-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis/CAT
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX-TOPO
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
KT
2.27.08
|
2.29.08
|-
|
pET-30 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-32 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-41 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-43.1 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-44 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-46 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-51 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pCDF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pRSF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBAC-2cp
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBACgus-2cp
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-30 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-32 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-52 3c/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pETDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pACYCDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pCOLADuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.28.07
|
11.27.07
|
11.28.07
|-
|
pCDFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pRSFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pETBlue-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pETBlue-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-3
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pF1A T7
|
Promega
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia
|
11.07.07
|
KT
2.27.08
|
2.29.08
|-
|
pF1K T7
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2A (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2K (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pF3A WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.28
|
02.29.08
|-
|
pF3K WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4A CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4K CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.20.08
|-
|
pF5A CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pF5K CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFN6A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.04.08
|
03.20.08
|-
|
pFN6K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC8A (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFC8K (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF9A CMV hRluc-neo
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN10A (ACT)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN11A (BIND)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF12A RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH
|

|-
|
pF12K RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
|

|-
|
PinPoint Xa-1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
03.02.08
|
03.17.08
|-
|
pALTER-EX1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pALTER-EX2
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pET-5a
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5b
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5c
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-1
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-2
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
PinPoint Control
|
Promega
|
Matthew Lakich
|
10.28.07
|
Miriam Garcia
|
11.05.07
|
1.10.08
|
01.23.08
|-
|
PinPoint Xa Control
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-2
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-3
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Z
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(+)
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(-)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-4Z
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(+)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(+)
|
Promega
|
Grant Richadson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-9Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-11Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-11Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-13Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGL2-Basic
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Control
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Enhancer
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Promoter
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pG5luc
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pSP64 Poly(A)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
|

|-
|
pSP72
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
|

|-
|
pSP73
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
KT
|

|-
|
pGEM-1
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
KT
|

|-
|
pGEM-2
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-3
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-4 Vector
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-15Zf(-)
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
12.13.07
|
01.16.07
|-
|
pSP64
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP65
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP70
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|

|

|-
|
pRS414
|
Simons Lab
|
Miriam Garcia
|
10.25.07
|

|

|
10.26.07
|
11.14.07
|-
|
pRS415
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.13.07
|
11.11.07
|
11.14.07
|-
|
pRS1274
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS591
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS552
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS551
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS577
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS550
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS308
|
Simons Lab
|
Amy Rabb
|
11.12.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS475
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || ||
|}
</protect>

pG5luc

2008-03-26T18:01:04Z

Trap18:

==At a Glance==
[[Image:pG5luc.gif|thumb|right|350px|pG5luc, image provided by [http://www.promega.com/figures/popup.asp?fn=1893va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pG5luc.txt Promega]
|-
!{{table heading style}} |Features
|
five GAL4 binding sites 

major late promoter of adenovirus 

predicted transcriptional start site 

luciferase (luc+) 

SV40 late polyadenylation signal 

beta-lactamase (Ampr) coding region 

phage f1 origin of replication 

upstream polyadenylation signal and transcriptional pause site 

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=40363816 AF264724]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.S47176b746562f&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pG5luc vector is part of a two-hybrid system. This two-hybrid system in particular is called the checkmate mammalian two-hybrid system and is extremely powerful when detecting protein:protein interactions in vivo. The main feature of the hybrid system is the modular domains found in some transcription factors. These domains are the DNA-binding domain and a transcriptional activation domain. The DNA-binding domain binds to specific DNA sequences and the transciptional activation domain interacts with the basal transcriptional machinery. RNA polymerase II complexes assembly is promoted at the TATA box and increases transcription when a transcriptional activation domain is in association with a DNA-binding domain. The Checkmate Mammalian two-hybrid system recieves the DNA-binding domain and the transcriptional activation domain from different and separate plasmids. They are closely associated when one protein fused to a DNA-binding domain interacts with a second protein fused to a transcriptional activation domain. The interaction between the two proteins results in transcription of a reporter gene.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Source==
[http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1195]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GGTACCGAGT TTCTAGACGG AGTACTGTCC TCCGAGCGGA GTACTGTCCT

51 CCGACTCGAG CGGAGTACTG TCCTCCGATC GGAGTACTGT CCTCCGCGAA

101 TTCCGGAGTA CTGTCCTCCG AAGACGCTAG CGGGGGGCTA TAAAAGGGGG

151 TGGGGGCGTT CGTCCTCACT CTAGATCTGC GATCTAAGTA AGCTTGGCAT

201 TCCGGTACTG TTGGTAAAGC CACCATGGAA GACGCCAAAA ACATAAAGAA

251 AGGCCCGGCG CCATTCTATC CGCTGGAAGA TGGAACCGCT GGAGAGCAAC

301 TGCATAAGGC TATGAAGAGA TACGCCCTGG TTCCTGGAAC AATTGCTTTT

351 ACAGATGCAC ATATCGAGGT GGACATCACT TACGCTGAGT ACTTCGAAAT

401 GTCCGTTCGG TTGGCAGAAG CTATGAAACG ATATGGGCTG AATACAAATC

451 ACAGAATCGT CGTATGCAGT GAAAACTCTC TTCAATTCTT TATGCCGGTG

501 TTGGGCGCGT TATTTATCGG AGTTGCAGTT GCGCCCGCGA ACGACATTTA

551 TAATGAACGT GAATTGCTCA ACAGTATGGG CATTTCGCAG CCTACCGTGG

601 TGTTCGTTTC CAAAAAGGGG TTGCAAAAAA TTTTGAACGT GCAAAAAAAG

651 CTCCCAATCA TCCAAAAAAT TATTATCATG GATTCTAAAA CGGATTACCA

701 GGGATTTCAG TCGATGTACA CGTTCGTCAC ATCTCATCTA CCTCCCGGTT

751 TTAATGAATA CGATTTTGTG CCAGAGTCCT TCGATAGGGA CAAGACAATT

801 GCACTGATCA TGAACTCCTC TGGATCTACT GGTCTGCCTA AAGGTGTCGC

851 TCTGCCTCAT AGAACTGCCT GCGTGAGATT CTCGCATGCC AGAGATCCTA

901 TTTTTGGCAA TCAAATCATT CCGGATACTG CGATTTTAAG TGTTGTTCCA

951 TTCCATCACG GTTTTGGAAT GTTTACTACA CTCGGATATT TGATATGTGG

1001 ATTTCGAGTC GTCTTAATGT ATAGATTTGA AGAAGAGCTG TTTCTGAGGA

1051 GCCTTCAGGA TTACAAGATT CAAAGTGCGC TGCTGGTGCC AACCCTATTC

1101 TCCTTCTTCG CCAAAAGCAC TCTGATTGAC AAATACGATT TATCTAATTT

1151 ACACGAAATT GCTTCTGGTG GCGCTCCCCT CTCTAAGGAA GTCGGGGAAG

1201 CGGTTGCCAA GAGGTTCCAT CTGCCAGGTA TCAGGCAAGG ATATGGGCTC

1251 ACTGAGACTA CATCAGCTAT TCTGATTACA CCCGAGGGGG ATGATAAACC

1301 GGGCGCGGTC GGTAAAGTTG TTCCATTTTT TGAAGCGAAG GTTGTGGATC

1351 TGGATACCGG GAAAACGCTG GGCGTTAATC AAAGAGGCGA ACTGTGTGTG

1401 AGAGGTCCTA TGATTATGTC CGGTTATGTA AACAATCCGG AAGCGACCAA

1451 CGCCTTGATT GACAAGGATG GATGGCTACA TTCTGGAGAC ATAGCTTACT

1501 GGGACGAAGA CGAACACTTC TTCATCGTTG ACCGCCTGAA GTCTCTGATT

1551 AAGTACAAAG GCTATCAGGT GGCTCCCGCT GAATTGGAAT CCATCTTGCT

1601 CCAACACCCC AACATCTTCG ACGCAGGTGT CGCAGGTCTT CCCGACGATG

1651 ACGCCGGTGA ACTTCCCGCC GCCGTTGTTG TTTTGGAGCA CGGAAAGACG

1701 ATGACGGAAA AAGAGATCGT GGATTACGTC GCCAGTCAAG TAACAACCGC

1751 GAAAAAGTTG CGCGGAGGAG TTGTGTTTGT GGACGAAGTA CCGAAAGGTC

1801 TTACCGGAAA ACTCGACGCA AGAAAAATCA GAGAGATCCT CATAAAGGCC

1851 AAGAAGGGCG GAAAGATCGC CGTGTAATTC TAGAGTCGGG GCGGCCGGCC

1901 GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA

1951 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT

2001 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA

2051 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT

2101 AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCGTCG

2151 ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC

2201 GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA

2251 CTCGTAGGAC AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT

2301 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG

2351 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT

2401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC

2451 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA

2501 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC

2551 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC

2601 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT

2651 CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA

2701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT

2751 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA

2801 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG

2851 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA

2901 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA

2951 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT

3001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG

3051 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA

3101 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT

3151 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT

3201 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA

3251 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA

3301 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA

3351 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG

3401 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC

3451 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG

3501 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA

3551 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG

3601 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG

3651 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG

3701 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG

3751 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA

3801 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG

3851 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG

3901 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC

3951 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT

4001 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG

4051 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT

4101 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT

4151 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT

4201 GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG

4251 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT

4301 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA

4351 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC

4401 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA

4451 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT

4501 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG

4551 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA

4601 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT

4651 AACGTTTACA ATTTCCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG

4701 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA

4751 TAAGTAAGTA ATATTAAGGT ACGGGAGGTA CTTGGAGCGG CCGCAATAAA

4801 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT

4851 AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA AACAAACTAG

4901 CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC ATTTCTCTAT

4951 CGATA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:Luc Gene]]
__NOTOC__

pG5luc

2008-03-26T18:00:26Z

Trap18: /* Source */

pG5luc

2008-03-26T17:59:53Z

Trap18: edited by Trap18 via TableEdit

==At a Glance==
[[Image:pG5luc.gif|thumb|right|350px|pG5luc, image provided by [http://www.promega.com/figures/popup.asp?fn=1893va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pG5luc.txt Promega]
|-
!{{table heading style}} |Features
|
five GAL4 binding sites 

major late promoter of adenovirus 

predicted transcriptional start site 

luciferase (luc+) 

SV40 late polyadenylation signal 

beta-lactamase (Ampr) coding region 

phage f1 origin of replication 

upstream polyadenylation signal and transcriptional pause site 

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=40363816 AF264724]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.S47176b746562f&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pG5luc vector is part of a two-hybrid system. This two-hybrid system in particular is called the checkmate mammalian two-hybrid system and is extremely powerful when detecting protein:protein interactions in vivo. The main feature of the hybrid system is the modular domains found in some transcription factors. These domains are the DNA-binding domain and a transcriptional activation domain. The DNA-binding domain binds to specific DNA sequences and the transciptional activation domain interacts with the basal transcriptional machinery. RNA polymerase II complexes assembly is promoted at the TATA box and increases transcription when a transcriptional activation domain is in association with a DNA-binding domain. The Checkmate Mammalian two-hybrid system recieves the DNA-binding domain and the transcriptional activation domain from different and separate plasmids. They are closely associated when one protein fused to a DNA-binding domain interacts with a second protein fused to a transcriptional activation domain. The interaction between the two proteins results in transcription of a reporter gene.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Source==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=CheckMate+Mammalian+Two%2DHybrid+System&description%5Ftext=CheckMate%26%23x2122%3B+Mammalian+Two%2DHybrid+System Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GGTACCGAGT TTCTAGACGG AGTACTGTCC TCCGAGCGGA GTACTGTCCT

51 CCGACTCGAG CGGAGTACTG TCCTCCGATC GGAGTACTGT CCTCCGCGAA

101 TTCCGGAGTA CTGTCCTCCG AAGACGCTAG CGGGGGGCTA TAAAAGGGGG

151 TGGGGGCGTT CGTCCTCACT CTAGATCTGC GATCTAAGTA AGCTTGGCAT

201 TCCGGTACTG TTGGTAAAGC CACCATGGAA GACGCCAAAA ACATAAAGAA

251 AGGCCCGGCG CCATTCTATC CGCTGGAAGA TGGAACCGCT GGAGAGCAAC

301 TGCATAAGGC TATGAAGAGA TACGCCCTGG TTCCTGGAAC AATTGCTTTT

351 ACAGATGCAC ATATCGAGGT GGACATCACT TACGCTGAGT ACTTCGAAAT

401 GTCCGTTCGG TTGGCAGAAG CTATGAAACG ATATGGGCTG AATACAAATC

451 ACAGAATCGT CGTATGCAGT GAAAACTCTC TTCAATTCTT TATGCCGGTG

501 TTGGGCGCGT TATTTATCGG AGTTGCAGTT GCGCCCGCGA ACGACATTTA

551 TAATGAACGT GAATTGCTCA ACAGTATGGG CATTTCGCAG CCTACCGTGG

601 TGTTCGTTTC CAAAAAGGGG TTGCAAAAAA TTTTGAACGT GCAAAAAAAG

651 CTCCCAATCA TCCAAAAAAT TATTATCATG GATTCTAAAA CGGATTACCA

701 GGGATTTCAG TCGATGTACA CGTTCGTCAC ATCTCATCTA CCTCCCGGTT

751 TTAATGAATA CGATTTTGTG CCAGAGTCCT TCGATAGGGA CAAGACAATT

801 GCACTGATCA TGAACTCCTC TGGATCTACT GGTCTGCCTA AAGGTGTCGC

851 TCTGCCTCAT AGAACTGCCT GCGTGAGATT CTCGCATGCC AGAGATCCTA

901 TTTTTGGCAA TCAAATCATT CCGGATACTG CGATTTTAAG TGTTGTTCCA

951 TTCCATCACG GTTTTGGAAT GTTTACTACA CTCGGATATT TGATATGTGG

1001 ATTTCGAGTC GTCTTAATGT ATAGATTTGA AGAAGAGCTG TTTCTGAGGA

1051 GCCTTCAGGA TTACAAGATT CAAAGTGCGC TGCTGGTGCC AACCCTATTC

1101 TCCTTCTTCG CCAAAAGCAC TCTGATTGAC AAATACGATT TATCTAATTT

1151 ACACGAAATT GCTTCTGGTG GCGCTCCCCT CTCTAAGGAA GTCGGGGAAG

1201 CGGTTGCCAA GAGGTTCCAT CTGCCAGGTA TCAGGCAAGG ATATGGGCTC

1251 ACTGAGACTA CATCAGCTAT TCTGATTACA CCCGAGGGGG ATGATAAACC

1301 GGGCGCGGTC GGTAAAGTTG TTCCATTTTT TGAAGCGAAG GTTGTGGATC

1351 TGGATACCGG GAAAACGCTG GGCGTTAATC AAAGAGGCGA ACTGTGTGTG

1401 AGAGGTCCTA TGATTATGTC CGGTTATGTA AACAATCCGG AAGCGACCAA

1451 CGCCTTGATT GACAAGGATG GATGGCTACA TTCTGGAGAC ATAGCTTACT

1501 GGGACGAAGA CGAACACTTC TTCATCGTTG ACCGCCTGAA GTCTCTGATT

1551 AAGTACAAAG GCTATCAGGT GGCTCCCGCT GAATTGGAAT CCATCTTGCT

1601 CCAACACCCC AACATCTTCG ACGCAGGTGT CGCAGGTCTT CCCGACGATG

1651 ACGCCGGTGA ACTTCCCGCC GCCGTTGTTG TTTTGGAGCA CGGAAAGACG

1701 ATGACGGAAA AAGAGATCGT GGATTACGTC GCCAGTCAAG TAACAACCGC

1751 GAAAAAGTTG CGCGGAGGAG TTGTGTTTGT GGACGAAGTA CCGAAAGGTC

1801 TTACCGGAAA ACTCGACGCA AGAAAAATCA GAGAGATCCT CATAAAGGCC

1851 AAGAAGGGCG GAAAGATCGC CGTGTAATTC TAGAGTCGGG GCGGCCGGCC

1901 GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA

1951 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT

2001 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA

2051 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT

2101 AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCGTCG

2151 ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC

2201 GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA

2251 CTCGTAGGAC AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT

2301 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG

2351 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT

2401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC

2451 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA

2501 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC

2551 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC

2601 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT

2651 CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA

2701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT

2751 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA

2801 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG

2851 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA

2901 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA

2951 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT

3001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG

3051 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA

3101 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT

3151 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT

3201 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA

3251 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA

3301 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA

3351 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG

3401 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC

3451 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG

3501 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA

3551 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG

3601 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG

3651 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG

3701 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG

3751 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA

3801 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG

3851 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG

3901 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC

3951 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT

4001 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG

4051 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT

4101 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT

4151 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT

4201 GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG

4251 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT

4301 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA

4351 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC

4401 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA

4451 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT

4501 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG

4551 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA

4601 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT

4651 AACGTTTACA ATTTCCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG

4701 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA

4751 TAAGTAAGTA ATATTAAGGT ACGGGAGGTA CTTGGAGCGG CCGCAATAAA

4801 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT

4851 AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA AACAAACTAG

4901 CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC ATTTCTCTAT

4951 CGATA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pG5luc

2008-03-26T17:56:46Z

Trap18: /* Notes */

==At a Glance==
[[Image:pG5luc.gif|thumb|right|350px|pG5luc, image provided by [http://www.promega.com/figures/popup.asp?fn=1893va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pG5luc.txt Promega]
|-
!{{table heading style}} |Features
|
This vector is a component of the CheckMate™ Mammalian Two-Hybrid System.

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=40363816 AF264724]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.S47176b746562f&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pG5luc vector is part of a two-hybrid system. This two-hybrid system in particular is called the checkmate mammalian two-hybrid system and is extremely powerful when detecting protein:protein interactions in vivo. The main feature of the hybrid system is the modular domains found in some transcription factors. These domains are the DNA-binding domain and a transcriptional activation domain. The DNA-binding domain binds to specific DNA sequences and the transciptional activation domain interacts with the basal transcriptional machinery. RNA polymerase II complexes assembly is promoted at the TATA box and increases transcription when a transcriptional activation domain is in association with a DNA-binding domain. The Checkmate Mammalian two-hybrid system recieves the DNA-binding domain and the transcriptional activation domain from different and separate plasmids. They are closely associated when one protein fused to a DNA-binding domain interacts with a second protein fused to a transcriptional activation domain. The interaction between the two proteins results in transcription of a reporter gene.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Source==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=CheckMate+Mammalian+Two%2DHybrid+System&description%5Ftext=CheckMate%26%23x2122%3B+Mammalian+Two%2DHybrid+System Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GGTACCGAGT TTCTAGACGG AGTACTGTCC TCCGAGCGGA GTACTGTCCT

51 CCGACTCGAG CGGAGTACTG TCCTCCGATC GGAGTACTGT CCTCCGCGAA

101 TTCCGGAGTA CTGTCCTCCG AAGACGCTAG CGGGGGGCTA TAAAAGGGGG

151 TGGGGGCGTT CGTCCTCACT CTAGATCTGC GATCTAAGTA AGCTTGGCAT

201 TCCGGTACTG TTGGTAAAGC CACCATGGAA GACGCCAAAA ACATAAAGAA

251 AGGCCCGGCG CCATTCTATC CGCTGGAAGA TGGAACCGCT GGAGAGCAAC

301 TGCATAAGGC TATGAAGAGA TACGCCCTGG TTCCTGGAAC AATTGCTTTT

351 ACAGATGCAC ATATCGAGGT GGACATCACT TACGCTGAGT ACTTCGAAAT

401 GTCCGTTCGG TTGGCAGAAG CTATGAAACG ATATGGGCTG AATACAAATC

451 ACAGAATCGT CGTATGCAGT GAAAACTCTC TTCAATTCTT TATGCCGGTG

501 TTGGGCGCGT TATTTATCGG AGTTGCAGTT GCGCCCGCGA ACGACATTTA

551 TAATGAACGT GAATTGCTCA ACAGTATGGG CATTTCGCAG CCTACCGTGG

601 TGTTCGTTTC CAAAAAGGGG TTGCAAAAAA TTTTGAACGT GCAAAAAAAG

651 CTCCCAATCA TCCAAAAAAT TATTATCATG GATTCTAAAA CGGATTACCA

701 GGGATTTCAG TCGATGTACA CGTTCGTCAC ATCTCATCTA CCTCCCGGTT

751 TTAATGAATA CGATTTTGTG CCAGAGTCCT TCGATAGGGA CAAGACAATT

801 GCACTGATCA TGAACTCCTC TGGATCTACT GGTCTGCCTA AAGGTGTCGC

851 TCTGCCTCAT AGAACTGCCT GCGTGAGATT CTCGCATGCC AGAGATCCTA

901 TTTTTGGCAA TCAAATCATT CCGGATACTG CGATTTTAAG TGTTGTTCCA

951 TTCCATCACG GTTTTGGAAT GTTTACTACA CTCGGATATT TGATATGTGG

1001 ATTTCGAGTC GTCTTAATGT ATAGATTTGA AGAAGAGCTG TTTCTGAGGA

1051 GCCTTCAGGA TTACAAGATT CAAAGTGCGC TGCTGGTGCC AACCCTATTC

1101 TCCTTCTTCG CCAAAAGCAC TCTGATTGAC AAATACGATT TATCTAATTT

1151 ACACGAAATT GCTTCTGGTG GCGCTCCCCT CTCTAAGGAA GTCGGGGAAG

1201 CGGTTGCCAA GAGGTTCCAT CTGCCAGGTA TCAGGCAAGG ATATGGGCTC

1251 ACTGAGACTA CATCAGCTAT TCTGATTACA CCCGAGGGGG ATGATAAACC

1301 GGGCGCGGTC GGTAAAGTTG TTCCATTTTT TGAAGCGAAG GTTGTGGATC

1351 TGGATACCGG GAAAACGCTG GGCGTTAATC AAAGAGGCGA ACTGTGTGTG

1401 AGAGGTCCTA TGATTATGTC CGGTTATGTA AACAATCCGG AAGCGACCAA

1451 CGCCTTGATT GACAAGGATG GATGGCTACA TTCTGGAGAC ATAGCTTACT

1501 GGGACGAAGA CGAACACTTC TTCATCGTTG ACCGCCTGAA GTCTCTGATT

1551 AAGTACAAAG GCTATCAGGT GGCTCCCGCT GAATTGGAAT CCATCTTGCT

1601 CCAACACCCC AACATCTTCG ACGCAGGTGT CGCAGGTCTT CCCGACGATG

1651 ACGCCGGTGA ACTTCCCGCC GCCGTTGTTG TTTTGGAGCA CGGAAAGACG

1701 ATGACGGAAA AAGAGATCGT GGATTACGTC GCCAGTCAAG TAACAACCGC

1751 GAAAAAGTTG CGCGGAGGAG TTGTGTTTGT GGACGAAGTA CCGAAAGGTC

1801 TTACCGGAAA ACTCGACGCA AGAAAAATCA GAGAGATCCT CATAAAGGCC

1851 AAGAAGGGCG GAAAGATCGC CGTGTAATTC TAGAGTCGGG GCGGCCGGCC

1901 GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA

1951 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT

2001 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA

2051 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT

2101 AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCGTCG

2151 ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC

2201 GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA

2251 CTCGTAGGAC AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT

2301 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG

2351 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT

2401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC

2451 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA

2501 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC

2551 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC

2601 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT

2651 CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA

2701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT

2751 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA

2801 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG

2851 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA

2901 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA

2951 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT

3001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG

3051 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA

3101 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT

3151 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT

3201 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA

3251 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA

3301 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA

3351 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG

3401 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC

3451 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG

3501 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA

3551 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG

3601 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG

3651 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG

3701 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG

3751 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA

3801 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG

3851 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG

3901 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC

3951 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT

4001 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG

4051 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT

4101 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT

4151 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT

4201 GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG

4251 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT

4301 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA

4351 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC

4401 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA

4451 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT

4501 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG

4551 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA

4601 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT

4651 AACGTTTACA ATTTCCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG

4701 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA

4751 TAAGTAAGTA ATATTAAGGT ACGGGAGGTA CTTGGAGCGG CCGCAATAAA

4801 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT

4851 AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA AACAAACTAG

4901 CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC ATTTCTCTAT

4951 CGATA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

EcoliWiki:Tracking Plasmids

2008-03-26T17:44:44Z

Trap18: edited by Trap18 via TableEdit

This is a page to track plasmids entered into the wiki. It is for developmental purposes only.

<protect>

{|class = 'sortable' {{Prettytable}} id='220572'
|-
!Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed!!Date Info Added!!Date Reviewed
|-
|
LITMUS 28i
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.03.07
|
11.12.07
|
11.13.07
|-
|
LITMUS 38i
|
NEB
|
Miriam Garcia
|
08.31.2007
|
Miriam Garcia
|
08.31.07
|
10.31.07
|
10.31.07
|-
|
pGPS1.1
|
NEB
|
Charner Williams
|
08.31.07
|
Miriam Garcia
|
09.04.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pGPS2.1
|
NEB
|
Matthew Lakich
|
09.04.07
|
Miriam Garcia
|
09.06.07
|
12.04.07
|
12.17.07
|-
|
pGPS3
|
NEB
|
Grant Richardson
|
09.07.07
|
Miriam Garcia
|
09.10.07
|
Aaron Hopkins
01.22.08
|
01.23.08
|-
|
pGPS4
|
NEB
|
Amy Rabb
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
Joseph Kim
01.22.08
|
01.23.08
|-
|
pGPS5
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
11.12.07
|
11.13.07
|-
|
pMAL-c4E
|
NEB
|
Amy Rabb
|
09.10.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.21.08
|
01.23.08
|-
|
pMAL-c4G
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.31.08
|
11.13.07
|-
|
pMAL-c4X
|
NEB
|
Matthew Lakich
|
9.13.07
|
Miriam Garcia
|
09.14.07
|
12.17.07 Miriam Garcia
|
12.17.07
|-
|
pMAL-p4E
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.07.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4G
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4X
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-pIII
|
NEB
|
Charner Williams
|
09.08.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pNEB193
|
NEB
|
CharnerWilliams
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pNEB206A
|
NEB
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 09.19.07
|
09.19.07
|-
|
pTWIN1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB3
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.12.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB11
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pUC19
|
NEB
|

|

|
Miriam Garcia
|
09.26.07
|
Aaron Hopkins
|

|-
|
pNEBR-R1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1GLuc
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1Hygro
|
NEB
|
Grant Richardson
|
09.10.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1-malE
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pCMV-GLuc
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pGLuc-Basic Vector
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-Q
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-Q
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-SET1
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET1-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SET1
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SET2
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEc-SET3
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1a
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1b
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.30.08
|-
|
pBEn-SBP-SET1c
|
Stratagene
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBPa
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPb
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPc
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pBEn-SET1a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.24.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-kc
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n-EK
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pCAL-n-FLAG
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pET-11a
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11b
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
02.05.08
|
02.07.08
|-
|
pET-11c
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11d
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3a
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.04.08
|
02.13.08
|-
|
pET-3b
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3c
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3d
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET100/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
09.10.07
|

|

|
11.01.07
|

|-
|
pET-14b
|
Novagen
|
Miriam Garcia
|
09.19.07
|

|

|
11.07.07
|

|-
|
pET-15b
|
Novagen
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Aaron Hopkins 02.05.08
|
02.13.08
|-
|
pET-16b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-17b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-19b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-20b(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21a(+)
|
Novagen
|
Matthew Lakich
|
10.2.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21b(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21c(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.25.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-22b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23a(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23b(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23c(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23d(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-24(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
02.13.08
|
02.14.08
|-
|
pET-24a(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24c(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-25b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Katrina Trapp
02.26.07
|
02.27.07
|-
|
pET-26b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-27b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28b(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-28c(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-29b(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29c(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Miriam Garcia
01.30.08
|
01.30.08
|-
|
pET-30a(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30c(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-31b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32a(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32c(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-33b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-39b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-40b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-41a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-42c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-45b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-47b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-48b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-49b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-50b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Pei Yap
02.09.08
|
02.13.08
|-
|
pET-51b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-52b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9a
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9b
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9c
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9d
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pETcoco-1
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pETcoco-2
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLacI
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysE
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysS
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET100/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D/LacZ
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160-GW/CAT
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160/GW/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161-GW/CAT
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161/GW/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pET-DEST42
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST15
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST14
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST17
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pDEST24
|
Invitrogen
|
Matthew Lakich
|
10.04.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pRSET T7
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pRSET-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pCRT7-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
2.29.08
|-
|
pBAD102/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD102/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
Pei 2.19.08
|
02.29.08
|-
|
pBAD-TOPO/LacZ/V5-His
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-DEST49
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
1.23.08
|-
|
pBAD/His B
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/His C
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/myc-His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His B
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His C
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/Myc-His/lacZ
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.17.08
|
01.23.08
|-
|
pBAD/gIII Calmodulin
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/gIII A
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII B
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII C
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.07
|
01.23.08
|-
|
pBAD/Thio-E
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pThioHis A
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
AH 02.28.08
|
03.04.08
|-
|
pThioHis B
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pThioHis C
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO/lacZ
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis2-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis2-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis/CAT
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX-TOPO
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
KT
2.27.08
|
2.29.08
|-
|
pET-30 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-32 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-41 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-43.1 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-44 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-46 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-51 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pCDF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pRSF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBAC-2cp
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBACgus-2cp
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-30 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-32 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-52 3c/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pETDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pACYCDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pCOLADuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.28.07
|
11.27.07
|
11.28.07
|-
|
pCDFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pRSFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pETBlue-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pETBlue-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-3
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pF1A T7
|
Promega
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia
|
11.07.07
|
KT
2.27.08
|
2.29.08
|-
|
pF1K T7
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2A (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2K (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pF3A WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.28
|
02.29.08
|-
|
pF3K WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4A CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4K CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.20.08
|-
|
pF5A CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pF5K CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFN6A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.04.08
|
03.20.08
|-
|
pFN6K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC8A (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFC8K (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF9A CMV hRluc-neo
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN10A (ACT)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN11A (BIND)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF12A RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH
|

|-
|
pF12K RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
|

|-
|
PinPoint Xa-1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
03.02.08
|
03.17.08
|-
|
pALTER-EX1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pALTER-EX2
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pET-5a
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5b
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5c
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-1
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-2
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
PinPoint Control
|
Promega
|
Matthew Lakich
|
10.28.07
|
Miriam Garcia
|
11.05.07
|
1.10.08
|
01.23.08
|-
|
PinPoint Xa Control
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-2
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-3
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Z
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(+)
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(-)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-4Z
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(+)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(+)
|
Promega
|
Grant Richadson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-9Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-11Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-11Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGEM-13Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
ML
|

|-
|
pGL2-Basic
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Control
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Enhancer
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
ML
|

|-
|
pGL2-Promoter
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pG5luc
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
|

|-
|
pSP64 Poly(A)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
|

|-
|
pSP72
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
|

|-
|
pSP73
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
KT
|

|-
|
pGEM-1
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
KT
|

|-
|
pGEM-2
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-3
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-4 Vector
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-15Zf(-)
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
12.13.07
|
01.16.07
|-
|
pSP64
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP65
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP70
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|

|

|-
|
pRS414
|
Simons Lab
|
Miriam Garcia
|
10.25.07
|

|

|
10.26.07
|
11.14.07
|-
|
pRS415
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.13.07
|
11.11.07
|
11.14.07
|-
|
pRS1274
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS591
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS552
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS551
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS577
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS550
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS308
|
Simons Lab
|
Amy Rabb
|
11.12.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS475
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || ||
|}
</protect>

pGL2-Promoter

2008-03-26T17:43:05Z

Trap18:

==At a Glance==
[[Image:pGL2-Promoter.gif|thumb|right|350px|pGL2-Promoter, image provided by [http://www.promega.com/figures/popup.asp?fn=0316va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-p.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region 

SV40 Promoter 

Luciferase gene(luc) 

GLprimer2 binding site 

RVprimer4 binding site 

ColE1-derived plasmid replication origin 

Beta-lactamase(Ampr) 

f1 origin 
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650342 X65326]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R47176b579c068&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The plasmid that contains that pGL2-Promoter Vector also contains a luciferase gene. The pGL-2 Promoter is located upstream from this gene. In the promoter-luc transcriptional unit DNA fragments can be inserted. These contain putative enhancer elements which can be inserted upstream or downstream from the promoter-luc unit.

The pGL2-Promoter along with other pGL2 luciferase reporter vectors are factors that could potentially regulate mammalian gene expression. These vectors provide a basis for quantitative analysis for these contributing factors. These factors include cis-acting or trans-acting. An example of the two factors would be promoters and enhancers for cis-acting and various DNA-binding factors for the trans-acting factors. Using the the firefly (Photinus pyralis) luciferase contained in the pGL2 vectors the transciptional activity in transfected cells can be monitored. The pGL2 vectors also contain features that are able to aid in the characterization and mutagenisis of the putative regulatory sequences.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_271]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATGATGTCT GGATCCGTCG ACCGATGCCC
2951 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC
3001 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC
3051 AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
3101 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
3151 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
3201 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
3251 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
3301 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
3351 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
3401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
3451 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
3501 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
3551 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
3601 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
3651 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
3701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
3751 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
3801 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
3851 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
3901 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
3951 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
4001 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
4051 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
4101 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
4151 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
4201 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
4251 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
4301 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
4351 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
4401 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
4451 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
4501 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
4551 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
4601 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
4651 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
4701 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
4751 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
4801 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
4851 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
4901 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
4951 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
5001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
5051 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
5101 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT
5151 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
5201 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
5251 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
5301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT
5351 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
5401 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA
5451 ATTTGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT
5501 GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA TAAGTAAGTA
5551 ATATTAAGGT ACGTGGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT
5601 CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT
5651 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
5701 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
5751 CATCAATGTA TCTTATGGTA CTGTAACTGA GCTAACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category: Luc Gene]]
__NOTOC__

pGL2-Promoter

2008-03-26T17:42:07Z

Trap18: /* Sources */

pGL2-Promoter

2008-03-26T17:41:13Z

Trap18: /* Notes */

==At a Glance==
[[Image:pGL2-Promoter.gif|thumb|right|350px|pGL2-Promoter, image provided by [http://www.promega.com/figures/popup.asp?fn=0316va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-p.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region 

SV40 Promoter 

Luciferase gene(luc) 

GLprimer2 binding site 

RVprimer4 binding site 

ColE1-derived plasmid replication origin 

Beta-lactamase(Ampr) 

f1 origin 
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650342 X65326]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R47176b579c068&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The plasmid that contains that pGL2-Promoter Vector also contains a luciferase gene. The pGL-2 Promoter is located upstream from this gene. In the promoter-luc transcriptional unit DNA fragments can be inserted. These contain putative enhancer elements which can be inserted upstream or downstream from the promoter-luc unit.

The pGL2-Promoter along with other pGL2 luciferase reporter vectors are factors that could potentially regulate mammalian gene expression. These vectors provide a basis for quantitative analysis for these contributing factors. These factors include cis-acting or trans-acting. An example of the two factors would be promoters and enhancers for cis-acting and various DNA-binding factors for the trans-acting factors. Using the the firefly (Photinus pyralis) luciferase contained in the pGL2 vectors the transciptional activity in transfected cells can be monitored. The pGL2 vectors also contain features that are able to aid in the characterization and mutagenisis of the putative regulatory sequences.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DPromoter+Vector&description%5Ftext=pGL2%2DPromoter+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATGATGTCT GGATCCGTCG ACCGATGCCC
2951 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC
3001 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC
3051 AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
3101 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
3151 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
3201 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
3251 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
3301 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
3351 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
3401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
3451 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
3501 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
3551 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
3601 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
3651 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
3701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
3751 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
3801 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
3851 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
3901 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
3951 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
4001 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
4051 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
4101 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
4151 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
4201 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
4251 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
4301 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
4351 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
4401 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
4451 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
4501 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
4551 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
4601 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
4651 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
4701 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
4751 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
4801 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
4851 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
4901 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
4951 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
5001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
5051 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
5101 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT
5151 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
5201 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
5251 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
5301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT
5351 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
5401 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA
5451 ATTTGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT
5501 GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA TAAGTAAGTA
5551 ATATTAAGGT ACGTGGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT
5601 CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT
5651 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
5701 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
5751 CATCAATGTA TCTTATGGTA CTGTAACTGA GCTAACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Promoter

2008-03-26T17:34:10Z

Trap18: edited by Trap18 via TableEdit

==At a Glance==
[[Image:pGL2-Promoter.gif|thumb|right|350px|pGL2-Promoter, image provided by [http://www.promega.com/figures/popup.asp?fn=0316va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-p.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region 

SV40 Promoter 

Luciferase gene(luc) 

GLprimer2 binding site 

RVprimer4 binding site 

ColE1-derived plasmid replication origin 

Beta-lactamase(Ampr) 

f1 origin 
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650342 X65326]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R47176b579c068&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The plasmid that contains that pGL2-Promoter Vector also contains a luciferase gene. The pGL-2 Promoter is located upstream from this gene. In the promoter-luc transcriptional unit DNA fragments can be inserted. These contain putative enhancer elements which can be inserted upstream or downstream from the promoter-luc unit.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DPromoter+Vector&description%5Ftext=pGL2%2DPromoter+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATGATGTCT GGATCCGTCG ACCGATGCCC
2951 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC
3001 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC
3051 AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
3101 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
3151 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
3201 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
3251 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
3301 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
3351 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
3401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
3451 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
3501 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
3551 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
3601 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
3651 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
3701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
3751 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
3801 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
3851 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
3901 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
3951 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
4001 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
4051 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
4101 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
4151 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
4201 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
4251 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
4301 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
4351 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
4401 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
4451 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
4501 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
4551 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
4601 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
4651 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
4701 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
4751 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
4801 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
4851 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
4901 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
4951 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
5001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
5051 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
5101 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT
5151 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
5201 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
5251 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
5301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT
5351 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
5401 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA
5451 ATTTGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT
5501 GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA TAAGTAAGTA
5551 ATATTAAGGT ACGTGGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT
5601 CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT
5651 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
5701 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
5751 CATCAATGTA TCTTATGGTA CTGTAACTGA GCTAACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Promoter

2008-03-26T17:33:40Z

Trap18: edited by Trap18 via TableEdit

==At a Glance==
[[Image:pGL2-Promoter.gif|thumb|right|350px|pGL2-Promoter, image provided by [http://www.promega.com/figures/popup.asp?fn=0316va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-p.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region 
SV40 Promoter 
Luciferase gene(luc) 
GLprimer2 binding site 
RVprimer4 binding site 
ColE1-derived plasmid replication origin 
Beta-lactamase(Ampr) 
f1 origin 
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650342 X65326]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R47176b579c068&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The plasmid that contains that pGL2-Promoter Vector also contains a luciferase gene. The pGL-2 Promoter is located upstream from this gene. In the promoter-luc transcriptional unit DNA fragments can be inserted. These contain putative enhancer elements which can be inserted upstream or downstream from the promoter-luc unit.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DPromoter+Vector&description%5Ftext=pGL2%2DPromoter+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATGATGTCT GGATCCGTCG ACCGATGCCC
2951 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC
3001 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC
3051 AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
3101 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
3151 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
3201 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
3251 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
3301 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
3351 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
3401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
3451 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
3501 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
3551 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
3601 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
3651 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
3701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
3751 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
3801 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
3851 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
3901 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
3951 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
4001 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
4051 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
4101 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
4151 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
4201 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
4251 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
4301 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
4351 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
4401 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
4451 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
4501 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
4551 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
4601 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
4651 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
4701 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
4751 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
4801 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
4851 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
4901 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
4951 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
5001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
5051 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
5101 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT
5151 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
5201 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
5251 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
5301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT
5351 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
5401 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA
5451 ATTTGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT
5501 GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA TAAGTAAGTA
5551 ATATTAAGGT ACGTGGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT
5601 CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT
5651 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
5701 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
5751 CATCAATGTA TCTTATGGTA CTGTAACTGA GCTAACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Promoter

2008-03-26T17:32:43Z

Trap18: edited by Trap18 via TableEdit

==At a Glance==
[[Image:pGL2-Promoter.gif|thumb|right|350px|pGL2-Promoter, image provided by [http://www.promega.com/figures/popup.asp?fn=0316va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-p.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region
SV40 Promoter
Luciferase gene(luc)
GLprimer2 binding site
RVprimer4 binding site
ColE1-derived plasmid replication origin
Beta-lactamase(Ampr)
f1 origin
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650342 X65326]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R47176b579c068&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The plasmid that contains that pGL2-Promoter Vector also contains a luciferase gene. The pGL-2 Promoter is located upstream from this gene. In the promoter-luc transcriptional unit DNA fragments can be inserted. These contain putative enhancer elements which can be inserted upstream or downstream from the promoter-luc unit.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DPromoter+Vector&description%5Ftext=pGL2%2DPromoter+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATGATGTCT GGATCCGTCG ACCGATGCCC
2951 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC
3001 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC
3051 AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
3101 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
3151 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
3201 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
3251 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
3301 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
3351 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
3401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
3451 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
3501 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
3551 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
3601 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
3651 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
3701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
3751 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
3801 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
3851 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
3901 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
3951 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
4001 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
4051 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
4101 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
4151 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
4201 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
4251 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
4301 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
4351 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
4401 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
4451 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
4501 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
4551 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
4601 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
4651 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
4701 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
4751 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
4801 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
4851 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
4901 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
4951 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
5001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
5051 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
5101 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT
5151 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
5201 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
5251 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
5301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT
5351 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
5401 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA
5451 ATTTGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT
5501 GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA TAAGTAAGTA
5551 ATATTAAGGT ACGTGGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT
5601 CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT
5651 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
5701 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
5751 CATCAATGTA TCTTATGGTA CTGTAACTGA GCTAACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Promoter

2008-03-26T17:29:07Z

Trap18: /* Notes */

==At a Glance==
[[Image:pGL2-Promoter.gif|thumb|right|350px|pGL2-Promoter, image provided by [http://www.promega.com/figures/popup.asp?fn=0316va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-p.txt Promega]
|-
!{{table heading style}} |Features
|
Contains the SV40 promoter upstream of the luc gene.

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650342 X65326]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R47176b579c068&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The plasmid that contains that pGL2-Promoter Vector also contains a luciferase gene. The pGL-2 Promoter is located upstream from this gene. In the promoter-luc transcriptional unit DNA fragments can be inserted. These contain putative enhancer elements which can be inserted upstream or downstream from the promoter-luc unit.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DPromoter+Vector&description%5Ftext=pGL2%2DPromoter+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATGATGTCT GGATCCGTCG ACCGATGCCC
2951 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC
3001 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC
3051 AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
3101 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
3151 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
3201 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
3251 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
3301 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
3351 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
3401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
3451 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
3501 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
3551 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
3601 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
3651 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
3701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
3751 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
3801 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
3851 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
3901 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
3951 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
4001 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
4051 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
4101 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
4151 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
4201 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
4251 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
4301 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
4351 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
4401 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
4451 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
4501 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
4551 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
4601 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
4651 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
4701 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
4751 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
4801 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
4851 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
4901 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
4951 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
5001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
5051 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
5101 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT
5151 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
5201 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
5251 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
5301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT
5351 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
5401 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA
5451 ATTTGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT
5501 GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA TAAGTAAGTA
5551 ATATTAAGGT ACGTGGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT
5601 CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT
5651 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
5701 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
5751 CATCAATGTA TCTTATGGTA CTGTAACTGA GCTAACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

EcoliWiki:Tracking Plasmids

2008-02-27T21:40:08Z

Trap18: edited by Trap18 via TableEdit

This is a page to track plasmids entered into the wiki. It is for developmental purposes only.

<protect>

{|class = 'sortable' {{Prettytable}} id='220572'
|-
!Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed!!Date Info Added!!Date Reviewed
|-
|
LITMUS 28i
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.03.07
|
11.12.07
|
11.13.07
|-
|
LITMUS 38i
|
NEB
|
Miriam Garcia
|
08.31.2007
|
Miriam Garcia
|
08.31.07
|
10.31.07
|
10.31.07
|-
|
pGPS1.1
|
NEB
|
Charner Williams
|
08.31.07
|
Miriam Garcia
|
09.04.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pGPS2.1
|
NEB
|
Matthew Lakich
|
09.04.07
|
Miriam Garcia
|
09.06.07
|
12.04.07
|
12.17.07
|-
|
pGPS3
|
NEB
|
Grant Richardson
|
09.07.07
|
Miriam Garcia
|
09.10.07
|
Aaron Hopkins
01.22.08
|
01.23.08
|-
|
pGPS4
|
NEB
|
Amy Rabb
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
Joseph Kim
01.22.08
|
01.23.08
|-
|
pGPS5
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
11.12.07
|
11.13.07
|-
|
pMAL-c4E
|
NEB
|
Amy Rabb
|
09.10.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.21.08
|
01.23.08
|-
|
pMAL-c4G
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.31.08
|
11.13.07
|-
|
pMAL-c4X
|
NEB
|
Matthew Lakich
|
9.13.07
|
Miriam Garcia
|
09.14.07
|
12.17.07 Miriam Garcia
|
12.17.07
|-
|
pMAL-p4E
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.07.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4G
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4X
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-pIII
|
NEB
|
Charner Williams
|
09.08.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pNEB193
|
NEB
|
CharnerWilliams
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pNEB206A
|
NEB
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 09.19.07
|
09.19.07
|-
|
pTWIN1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB3
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.12.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB11
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pUC19
|
NEB
|

|

|
Miriam Garcia
|
09.26.07
|
Aaron Hopkins
|

|-
|
pNEBR-R1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1GLuc
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1Hygro
|
NEB
|
Grant Richardson
|
09.10.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1-malE
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pCMV-GLuc
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pGLuc-Basic Vector
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-Q
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-Q
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-SET1
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET1-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SET1
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SET2
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEc-SET3
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1a
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1b
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.30.08
|-
|
pBEn-SBP-SET1c
|
Stratagene
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBPa
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPb
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPc
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pBEn-SET1a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.24.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-kc
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n-EK
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pCAL-n-FLAG
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pET-11a
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11b
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
02.05.08
|
02.07.08
|-
|
pET-11c
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11d
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3a
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.04.08
|
02.13.08
|-
|
pET-3b
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3c
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3d
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET100/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
09.10.07
|

|

|
11.01.07
|

|-
|
pET-14b
|
Novagen
|
Miriam Garcia
|
09.19.07
|

|

|
11.07.07
|

|-
|
pET-15b
|
Novagen
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Aaron Hopkins 02.05.08
|
02.13.08
|-
|
pET-16b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-17b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-19b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-20b(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21a(+)
|
Novagen
|
Matthew Lakich
|
10.2.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21b(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21c(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.25.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-22b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23a(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23b(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23c(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23d(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-24(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
02.13.08
|
02.14.08
|-
|
pET-24a(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24c(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-25b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Katrina Trapp
02.26.07
|
02.27.07
|-
|
pET-26b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-27b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28b(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-28c(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-29b(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29c(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Miriam Garcia
01.30.08
|
01.30.08
|-
|
pET-30a(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30c(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-31b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32a(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32c(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-33b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-39b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-40b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-41a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-42c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-45b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-47b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-48b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-49b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-50b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Pei Yap
02.09.08
|
02.13.08
|-
|
pET-51b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-52b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9a
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9b
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9c
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9d
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pETcoco-1
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pETcoco-2
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLacI
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysE
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysS
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET100/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D/LacZ
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160-GW/CAT
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160/GW/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161-GW/CAT
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161/GW/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|

|-
|
pET-DEST42
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|

|-
|
pDEST15
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|

|-
|
pDEST14
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|

|-
|
pDEST17
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pDEST24
|
Invitrogen
|
Matthew Lakich
|
10.04.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pRSET T7
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|

|-
|
pRSET-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|

|-
|
pCRT7-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|

|-
|
pBAD102/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|

|-
|
pBAD102/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|

|-
|
pBAD202/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|

|-
|
pBAD202/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|

|-
|
pBAD/Thio
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|

|-
|
pBAD/Thio-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|

|-
|
pBAD-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
AH
|

|-
|
pBAD-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
Pei 2.19.08
|

|-
|
pBAD-TOPO/LacZ/V5-His
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH
|

|-
|
pBAD-DEST49
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH
|

|-
|
pBAD/His/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH
|

|-
|
pBAD/His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
1.23.08
|-
|
pBAD/His B
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/His C
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/myc-His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His B
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His C
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/Myc-His/lacZ
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.17.08
|
01.23.08
|-
|
pBAD/gIII Calmodulin
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
AH
|

|-
|
pBAD/gIII A
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII B
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII C
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.07
|
01.23.08
|-
|
pBAD/Thio-E
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pThioHis A
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
AH
|

|-
|
pThioHis B
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|

|-
|
pThioHis C
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|

|-
|
pTrcHis-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|

|-
|
pTrcHis-TOPO/lacZ
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|

|-
|
pTrcHis2-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|

|-
|
pTrcHis2-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|

|-
|
pTrcHis/CAT
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|

|-
|
pTrcHis A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|

|-
|
pTrcHis B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|

|-
|
pTrcHis C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pTrcHis2 C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
ML
|

|-
|
pCX-TOPO
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
KT
2.27.08
|

|-
|
pET-30 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-32 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-41 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-43.1 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-44 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-46 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-51 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pCDF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pRSF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBAC-2cp
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBACgus-2cp
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-30 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-32 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-52 3c/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pETDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pACYCDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pCOLADuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.28.07
|
11.27.07
|
11.28.07
|-
|
pCDFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pRSFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pETBlue-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pETBlue-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-3
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pF1A T7
|
Promega
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia
|
11.07.07
|
KT
2.27.08
|

|-
|
pF1K T7
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|

|-
|
pFN2A (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|

|-
|
pFN2K (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|

|-
|
pF3A WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.28
|

|-
|
pF3K WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY
|

|-
|
pF4A CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY
|

|-
|
pF4K CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY
|

|-
|
pF5A CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY
|

|-
|
pF5K CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY
|

|-
|
pFN6A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY
|

|-
|
pFN6K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY
|

|-
|
pFC7A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY
|

|-
|
pFC7K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY
|

|-
|
pFC8A (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|

|

|-
|
pFC8K (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|

|

|-
|
pF9A CMV hRluc-neo
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|

|

|-
|
pFN10A (ACT)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|

|

|-
|
pFN11A (BIND)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|

|

|-
|
pF12A RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|

|

|-
|
pF12K RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|

|

|-
|
PinPoint Xa-1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|

|

|-
|
pALTER-EX1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pALTER-EX2
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pET-5a
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5b
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5c
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-1
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-2
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
PinPoint Control
|
Promega
|
Matthew Lakich
|
10.28.07
|
Miriam Garcia
|
11.05.07
|
1.10.08
|
01.23.08
|-
|
PinPoint Xa Control
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-2
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-3
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Z
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(+)
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(-)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|

|

|-
|
pGEM-4Z
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|

|

|-
|
pGEM-5Zf(+)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|

|

|-
|
pGEM-5Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|

|

|-
|
pGEM-7Zf(+)
|
Promega
|
Grant Richadson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|

|

|-
|
pGEM-7Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|

|

|-
|
pGEM-9Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|

|

|-
|
pGEM-11Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|

|

|-
|
pGEM-11Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|

|

|-
|
pGEM-13Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|

|

|-
|
pGL2-Basic
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|

|

|-
|
pGL2-Control
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|

|

|-
|
pGL2-Enhancer
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|

|

|-
|
pGL2-Promoter
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|

|

|-
|
pG5luc
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|

|

|-
|
pSP64 Poly(A)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|

|

|-
|
pSP72
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|

|

|-
|
pSP73
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-1
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
12.13.07
|
01.16.07
|-
|
pGEM-2
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-3
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-4 Vector
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-15Zf(-)
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
12.13.07
|
01.16.07
|-
|
pSP64
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP65
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP70
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|

|

|-
|
pRS414
|
Simons Lab
|
Miriam Garcia
|
10.25.07
|

|

|
10.26.07
|
11.14.07
|-
|
pRS415
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.13.07
|
11.11.07
|
11.14.07
|-
|
pRS1274
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS591
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS552
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS551
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS577
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS550
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS308
|
Simons Lab
|
Amy Rabb
|
11.12.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS475
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || ||
|}
</protect>

pF3A WG (BYDV)

2008-02-27T21:39:42Z

Trap18: /* Sources */

==At a Glance==
[[Image:#pF3A WG (BYDV).gif|thumb|right|250px|pF3A WG (BYDV), image provided by [http://www.promega.com/figures/popup.asp?fn=4910ma&partno=L5671&product=pF3A+WG+%28BYDV%29+Flexi%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E+Vector Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pf3a_wg_bydv.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 promoter 
T7 promoter 
BYDV 5' UTR 
Sgf I site 
Barnase coding region 
Pme I site 
BYDV 3' TE 
T7 terminator 
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=62275796 AY949043]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.M470e705a29963&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pF3A WG(BYDV) vector is one of two vectors inthe Wheat Germ Extract Plus. The Wheat Germ Extrast Plus is a highly efficient, single tube protein expression. In the extract exists all cellular substances that are required for protein synthesis including tRNA, ribosomes, amino acids, initiation factors, elongation factors, and termination factors.To enhance the translation of a wide range of RNAs potassium acetate is added at a certain concentration. The initation of protein synthesis is started by the addition of an mRNA template and the reaction is then incubated for 2 hours.

The vectors pF3A WG (BYDV) and pF3K WG (BYDV) are both designed for in vitro expression in wheat germ extracts. Contained in vectors are sequences from barley yellow dwarf virus (BYDV) and RNA plant virus. They are located downstream and upstream of the protein coding region of interest.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
This vector is also part of the Flexi Cloning System. It is including in this group of vectors.

==Sources==
[http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1645 Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 GGATCTCGAT CCCGCGAAAT TAATTTAGGT GACACTATAG AAGGGTAATA

51 CGACTCACTA TAGCAGTGAA GATTGACCAT CTCACAAAAG CTGTTACGTG

101 CTTGTAACAC ACTACACACT CGTTTTGTAT TCGAGAAGTA GTTGCAACAA

151 CGGTCCCCTT ATTGCCTGAC AAGCTGAGGG CCACCCTTCT ATCCCCACCG

201 CGCGATCGCC ATGGAATAAG TAAGGAATCC ACATGGCACA GGTTATCAAC

251 ACGTTTGACG GGGTTGCGGA TTATCTTCAG ACATATCATA AGCTACCTGA

301 TAATTACATT ACAAAATCAG AAGCACAAGC CCTCGGCTGG GTGGCATCAA

351 AAGGGAACCT TGCAGACGTC GCTCCGGGGA AAAGCATCGG CGGAGACATC

401 TTCTCAAACA GGGAAGGCAA ACTCCAGGGC AAAAGCGGAC GAACATGGCG

451 TGAAGCGGAT ATTAACTATA CATCAGGCTT CAGAAATTCA GACCGGATTC

501 TTTACTCAAG CGACTGGCTG ATTTACAAAA CAACGGACCA TTATCAGACC

551 TTTACAAAAA TCAGATAATG TTTAAACGAA TTCGAGCTCG GTACCAGTGA

601 AGACAACACC ACTAGCACAA ATCGGATCCT GGGAAACAGG CAGAACTTCG

651 GTTCATAAGC TCGGGTAGGC TGTCAACCTA CCGCCGTATC GTATTGTGTT

701 TGTCTAGATA CGTATCGCGA GTCGACCTGC AGGCATGCAA GCTGATCCGG

751 CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG

801 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT

851 TTTGCTGAAA GGAGGAACTA TATCCGGTTC GCTTGCTGTC CATAAAACCG

901 CCCAGTCTAG CTATCGCCAT GTAAGCCCAC TGCAAGCTAC CTGCTTTCTC

951 TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT

1001 CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTA ATGGTTTCTT

1051 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT

1101 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC

1151 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC

1201 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT

1251 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT

1301 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC

1351 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTCAAA

1401 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA

1451 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC

1501 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC

1551 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC

1601 AACTATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG

1651 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA

1701 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT

1751 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT

1801 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG

1851 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG

1901 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC

1951 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA

2001 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAATT

2051 CGAAATGACC GACCAAGCGA CGCCCAACCG GTATCAGCTC ACTCAAAGGC

2101 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT

2151 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG

2201 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG

2251 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

2301 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

2351 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA

2401 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC

2451 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC

2501 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT

2551 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

2601 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA

2651 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT

2701 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT

2751 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATT TCAAGAAGAT

2801 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG

2851 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

2901 TTTTATAGTC CGGAAATACA GGAACGCACG CTGGATGGCC CTTCGCTGGG

2951 ATGGTGAAAC CATGAAAAAT GGCAGCTTCA GTGGATTAAG TGGGGGTAAT

3001 GTGGCCTGTA CCCTCTGGTT GCATAGGTAT TCATACGGTT AAAATTTATC

3051 AGGCGCGATT GCGGCAGTTT TTCGGGTGGT TTGTTGCCAT TTTTACCTGT

3101 CTGCTGCCGT GATCGCGCTG AACGCGTTTT AGCGGTGCGT ACAATTAAGG

3151 GATTATGGTA AATCCACTTA CTGTCTGCCC TCGTAGCCAT CGAGATAAAC

3201 CGCAGTACTC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTTTTC

3251 AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA

3301 TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT

3351 CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG

3401 AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG

3451 ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT

3501 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG

3551 AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC

3601 AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTTG

3651 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCAT

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Promega Plasmids]][[Category:Ampicillin Marker Plasmids]]
__NOTOC__

pF3A WG (BYDV)

2008-02-27T21:39:14Z

Trap18: edited by Trap18 via TableEdit

==At a Glance==
[[Image:#pF3A WG (BYDV).gif|thumb|right|250px|pF3A WG (BYDV), image provided by [http://www.promega.com/figures/popup.asp?fn=4910ma&partno=L5671&product=pF3A+WG+%28BYDV%29+Flexi%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E+Vector Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pf3a_wg_bydv.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 promoter 
T7 promoter 
BYDV 5' UTR 
Sgf I site 
Barnase coding region 
Pme I site 
BYDV 3' TE 
T7 terminator 
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=62275796 AY949043]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.M470e705a29963&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pF3A WG(BYDV) vector is one of two vectors inthe Wheat Germ Extract Plus. The Wheat Germ Extrast Plus is a highly efficient, single tube protein expression. In the extract exists all cellular substances that are required for protein synthesis including tRNA, ribosomes, amino acids, initiation factors, elongation factors, and termination factors.To enhance the translation of a wide range of RNAs potassium acetate is added at a certain concentration. The initation of protein synthesis is started by the addition of an mRNA template and the reaction is then incubated for 2 hours.

The vectors pF3A WG (BYDV) and pF3K WG (BYDV) are both designed for in vitro expression in wheat germ extracts. Contained in vectors are sequences from barley yellow dwarf virus (BYDV) and RNA plant virus. They are located downstream and upstream of the protein coding region of interest.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
This vector is also part of the Flexi Cloning System. It is including in this group of vectors.

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=Flexi+Cloning+System&description%5Ftext=Flexi%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E+Cloning+System Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 GGATCTCGAT CCCGCGAAAT TAATTTAGGT GACACTATAG AAGGGTAATA

51 CGACTCACTA TAGCAGTGAA GATTGACCAT CTCACAAAAG CTGTTACGTG

101 CTTGTAACAC ACTACACACT CGTTTTGTAT TCGAGAAGTA GTTGCAACAA

151 CGGTCCCCTT ATTGCCTGAC AAGCTGAGGG CCACCCTTCT ATCCCCACCG

201 CGCGATCGCC ATGGAATAAG TAAGGAATCC ACATGGCACA GGTTATCAAC

251 ACGTTTGACG GGGTTGCGGA TTATCTTCAG ACATATCATA AGCTACCTGA

301 TAATTACATT ACAAAATCAG AAGCACAAGC CCTCGGCTGG GTGGCATCAA

351 AAGGGAACCT TGCAGACGTC GCTCCGGGGA AAAGCATCGG CGGAGACATC

401 TTCTCAAACA GGGAAGGCAA ACTCCAGGGC AAAAGCGGAC GAACATGGCG

451 TGAAGCGGAT ATTAACTATA CATCAGGCTT CAGAAATTCA GACCGGATTC

501 TTTACTCAAG CGACTGGCTG ATTTACAAAA CAACGGACCA TTATCAGACC

551 TTTACAAAAA TCAGATAATG TTTAAACGAA TTCGAGCTCG GTACCAGTGA

601 AGACAACACC ACTAGCACAA ATCGGATCCT GGGAAACAGG CAGAACTTCG

651 GTTCATAAGC TCGGGTAGGC TGTCAACCTA CCGCCGTATC GTATTGTGTT

701 TGTCTAGATA CGTATCGCGA GTCGACCTGC AGGCATGCAA GCTGATCCGG

751 CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG

801 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT

851 TTTGCTGAAA GGAGGAACTA TATCCGGTTC GCTTGCTGTC CATAAAACCG

901 CCCAGTCTAG CTATCGCCAT GTAAGCCCAC TGCAAGCTAC CTGCTTTCTC

951 TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT

1001 CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTA ATGGTTTCTT

1051 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT

1101 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC

1151 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC

1201 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT

1251 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT

1301 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC

1351 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTCAAA

1401 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA

1451 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC

1501 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC

1551 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC

1601 AACTATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG

1651 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA

1701 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT

1751 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT

1801 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG

1851 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG

1901 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC

1951 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA

2001 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAATT

2051 CGAAATGACC GACCAAGCGA CGCCCAACCG GTATCAGCTC ACTCAAAGGC

2101 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT

2151 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG

2201 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG

2251 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

2301 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

2351 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA

2401 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC

2451 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC

2501 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT

2551 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

2601 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA

2651 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT

2701 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT

2751 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATT TCAAGAAGAT

2801 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG

2851 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

2901 TTTTATAGTC CGGAAATACA GGAACGCACG CTGGATGGCC CTTCGCTGGG

2951 ATGGTGAAAC CATGAAAAAT GGCAGCTTCA GTGGATTAAG TGGGGGTAAT

3001 GTGGCCTGTA CCCTCTGGTT GCATAGGTAT TCATACGGTT AAAATTTATC

3051 AGGCGCGATT GCGGCAGTTT TTCGGGTGGT TTGTTGCCAT TTTTACCTGT

3101 CTGCTGCCGT GATCGCGCTG AACGCGTTTT AGCGGTGCGT ACAATTAAGG

3151 GATTATGGTA AATCCACTTA CTGTCTGCCC TCGTAGCCAT CGAGATAAAC

3201 CGCAGTACTC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTTTTC

3251 AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA

3301 TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT

3351 CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG

3401 AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG

3451 ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT

3501 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG

3551 AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC

3601 AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTTG

3651 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCAT

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Promega Plasmids]][[Category:Ampicillin Marker Plasmids]]
__NOTOC__

pF3A WG (BYDV)

2008-02-27T21:38:51Z

Trap18: edited by Trap18 via TableEdit

==At a Glance==
[[Image:#pF3A WG (BYDV).gif|thumb|right|250px|pF3A WG (BYDV), image provided by [http://www.promega.com/figures/popup.asp?fn=4910ma&partno=L5671&product=pF3A+WG+%28BYDV%29+Flexi%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E+Vector Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|

|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pf3a_wg_bydv.txt Promega]
|-
!{{table heading style}} |Features
|
SP6 RNA polymerase promoter 
T7 RNA polymerase promoter 
BYDV 5' UTR 
Sgf I site 
Barnase coding region 
Pme I site 
BYDV 3' TE 
T7 terminator 
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=62275796 AY949043]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.M470e705a29963&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pF3A WG(BYDV) vector is one of two vectors inthe Wheat Germ Extract Plus. The Wheat Germ Extrast Plus is a highly efficient, single tube protein expression. In the extract exists all cellular substances that are required for protein synthesis including tRNA, ribosomes, amino acids, initiation factors, elongation factors, and termination factors.To enhance the translation of a wide range of RNAs potassium acetate is added at a certain concentration. The initation of protein synthesis is started by the addition of an mRNA template and the reaction is then incubated for 2 hours.

The vectors pF3A WG (BYDV) and pF3K WG (BYDV) are both designed for in vitro expression in wheat germ extracts. Contained in vectors are sequences from barley yellow dwarf virus (BYDV) and RNA plant virus. They are located downstream and upstream of the protein coding region of interest.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
This vector is also part of the Flexi Cloning System. It is including in this group of vectors.

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=Flexi+Cloning+System&description%5Ftext=Flexi%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E+Cloning+System Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 GGATCTCGAT CCCGCGAAAT TAATTTAGGT GACACTATAG AAGGGTAATA

51 CGACTCACTA TAGCAGTGAA GATTGACCAT CTCACAAAAG CTGTTACGTG

101 CTTGTAACAC ACTACACACT CGTTTTGTAT TCGAGAAGTA GTTGCAACAA

151 CGGTCCCCTT ATTGCCTGAC AAGCTGAGGG CCACCCTTCT ATCCCCACCG

201 CGCGATCGCC ATGGAATAAG TAAGGAATCC ACATGGCACA GGTTATCAAC

251 ACGTTTGACG GGGTTGCGGA TTATCTTCAG ACATATCATA AGCTACCTGA

301 TAATTACATT ACAAAATCAG AAGCACAAGC CCTCGGCTGG GTGGCATCAA

351 AAGGGAACCT TGCAGACGTC GCTCCGGGGA AAAGCATCGG CGGAGACATC

401 TTCTCAAACA GGGAAGGCAA ACTCCAGGGC AAAAGCGGAC GAACATGGCG

451 TGAAGCGGAT ATTAACTATA CATCAGGCTT CAGAAATTCA GACCGGATTC

501 TTTACTCAAG CGACTGGCTG ATTTACAAAA CAACGGACCA TTATCAGACC

551 TTTACAAAAA TCAGATAATG TTTAAACGAA TTCGAGCTCG GTACCAGTGA

601 AGACAACACC ACTAGCACAA ATCGGATCCT GGGAAACAGG CAGAACTTCG

651 GTTCATAAGC TCGGGTAGGC TGTCAACCTA CCGCCGTATC GTATTGTGTT

701 TGTCTAGATA CGTATCGCGA GTCGACCTGC AGGCATGCAA GCTGATCCGG

751 CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG

801 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT

851 TTTGCTGAAA GGAGGAACTA TATCCGGTTC GCTTGCTGTC CATAAAACCG

901 CCCAGTCTAG CTATCGCCAT GTAAGCCCAC TGCAAGCTAC CTGCTTTCTC

951 TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT

1001 CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTA ATGGTTTCTT

1051 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT

1101 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC

1151 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC

1201 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT

1251 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT

1301 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC

1351 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTCAAA

1401 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA

1451 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC

1501 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC

1551 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC

1601 AACTATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG

1651 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA

1701 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT

1751 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT

1801 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG

1851 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG

1901 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC

1951 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA

2001 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAATT

2051 CGAAATGACC GACCAAGCGA CGCCCAACCG GTATCAGCTC ACTCAAAGGC

2101 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT

2151 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG

2201 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG

2251 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

2301 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

2351 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA

2401 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC

2451 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC

2501 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT

2551 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

2601 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA

2651 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT

2701 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT

2751 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATT TCAAGAAGAT

2801 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG

2851 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

2901 TTTTATAGTC CGGAAATACA GGAACGCACG CTGGATGGCC CTTCGCTGGG

2951 ATGGTGAAAC CATGAAAAAT GGCAGCTTCA GTGGATTAAG TGGGGGTAAT

3001 GTGGCCTGTA CCCTCTGGTT GCATAGGTAT TCATACGGTT AAAATTTATC

3051 AGGCGCGATT GCGGCAGTTT TTCGGGTGGT TTGTTGCCAT TTTTACCTGT

3101 CTGCTGCCGT GATCGCGCTG AACGCGTTTT AGCGGTGCGT ACAATTAAGG

3151 GATTATGGTA AATCCACTTA CTGTCTGCCC TCGTAGCCAT CGAGATAAAC

3201 CGCAGTACTC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTTTTC

3251 AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA

3301 TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT

3351 CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG

3401 AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG

3451 ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT

3501 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG

3551 AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC

3601 AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTTG

3651 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCAT

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Promega Plasmids]][[Category:Ampicillin Marker Plasmids]]
__NOTOC__