EcoliWiki - User contributions [en]

xanQ:Gene Product(s)

2010-07-24T06:59:21Z

Stathismar: Table edited by Stathismar via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|xanQ}}{{Tab|Gene|xanQ}}{{Tab On|Gene Product(s)|xanQ}}{{Tab|Expression|xanQ}}{{Tab|Evolution|xanQ}}{{Tab|On One Page|xanQ}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==

{{Product_nomenclature_help}}
{| id="H46aac44548aff" class=" tableEdit Product_nomenclature_table"

|-
!align=left {{table heading style}} |Standard name
||
XanQ
|-
!align=left {{table heading style}} |Synonyms
||
predicted transporter<ref name='LIB:Riley_2006'/>, B2882<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YgfO<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/><ref name='PMID:19581302'/>
|-
!align=left {{table heading style}} |Product description
||
YgfO NCS2 transporter<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Xanthine permease<ref name='LIB:EcoGene'/> <ref name='PMID:16096267'/>
|-
!align=left {{table heading style}} |EC number (for enzymes)
||

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.H46aac44548aff&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}


<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> 
{{Product_GO_help}}
{| id="I46aac44655e93" class=" tableEdit GO_table_product dataTable"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
GO:0005887
|
integral to plasma membrane
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0042906
|
xanthine transport
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0042907
|
xanthine transmembrane transporter activity
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0055085
|
transmembrane transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR006042
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0055085
|
transmembrane transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR006043
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.I46aac44655e93&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>

{{Product_interactions_help}}
{| id="W46aac44961f80" class=" tableEdit Product_interactions_table dataTable"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.W46aac44961f80&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || || || ||
|}


</protect>
===Notes===

==Localization==

{{Product_localization_help}}
{| id="O46aac44a29180" class=" tableEdit Product_localization_table"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Source!!Notes
|-
|
plasma membrane
|
C-terminus localized in the cytoplasm with 12 predicted transmembrane domains
|
Daley et al. (2005) <ref name=PMID:15919996/>
|

|

|-
|
plasma membrane
|

|

|

|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.O46aac44a29180&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table] || || || ||
|}


<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>

{{Product_physical_properties_help}}
{| id="N46aac44bbe1ce" class=" tableEdit Product_phys_prop_table"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=YGFO-MONOMER at EcoCyc]
<pre>M SDINHAGSDL IFELEDRPPF HQALVGAITH LLAIFVPMVT
PALIVGAALQ LSAETTAYLV SMAMIASGIG TWLQVNRYGI VGSGLLSIQS VNFSFVTVMI
ALGSSMKSDG FHEELIMSSL LGVSFVGAFL VVGSSFILPY LRRVITPTVS GIVVLMIGLS
LIKVGIIDFG GGFAAKSSGT FGNYEHLGVG LLVLIVVIGF NCCRSPLLRM GGIAIGLCVG
YIASLCLGMV DFSSMRNLPL ITIPHPFKYG FSFSFHQFLV VGTIYLLSVL EAVGDITATA
MVSRRPIQGE EYQSRLKGGV LADGLVSVIA SAVGSLPLTT FAQNNGVIQM TGVASRYVGR
TIAVMLVILG LFPMIGGFFT TIPSAVLGGA MTLMFSMIAI AGIRIIITNG LKRRETLIVA
TSLGLGLGVS YDPEIFKILP ASIYVLVENP ICAGGLTAIL LNIILPGGYR QENVLPGITS
AEEMD</pre>
|-
!align=left {{table heading style}} |Length
||
466
|-
!align=left {{table heading style}} |Mol. Wt
||
49.109 kDa
|-
!align=left {{table heading style}} |pI
||
6.9 (calculated)
|-
!align=left {{table heading style}} |Extinction coefficient
||
23,380 - 24,005 (calc based on 12 Y, 1 W, and 5 C residues)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.N46aac44bbe1ce&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_phys_prop_table edit table] ||
|}


<big>'''Domains/Motifs/Modification Sites'''</big>
<table>
<tr align="left" valign="top">
<td>


{{Product_domains_motifs_help}}
{| id="Q46aac44c3a551" class=" tableEdit Product_motif_table dataTable"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes!!References
|-
|
Domain
|
22..416
|
[http://pfam.janelia.org/family/PF00860 PF00860 Permease family]
|

|
PMID:19920124

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.Q46aac44c3a551&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table] || || || ||
|}


</td>
<td>


<motif_map/>
{{TMHMM Picture|ygfO}}
</td>
</tr>
</table>

<table><tr valign='top'><td>
<big>'''Structure'''</big> 

{{Product_structure_help}}
{| id="U46a85a1f3d659" class=" tableEdit Product_structure_table"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=ygfO
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.U46a85a1f3d659&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}


</td><td></protect>
==== Structure figures ====

<protect>
</td><tr><table></protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}

{{Product_resources_help}}
{| id="O46aac44d059a9" class=" tableEdit Product_resource_table"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.O46aac44d059a9&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table] || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="H46aac44d64a0c" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=YGFO-MONOMER EcoCyc:YGFO-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P67444|UniProt:P67444]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|

|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417358 RefSeq:NP_417358]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.H46aac44d64a0c&page=26127&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="G46a85a21ead26" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.G46a85a21ead26&page=26127&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0015292_!_uniporter activity]][[Category:Location:plasma membrane]]
[[Category:Pfam:PF00860_!_Permease family]]

xanQ:Quickview

2010-07-24T06:57:17Z

Stathismar: Table edited by Stathismar via TableEdit

xanQ:Gene Product(s)

2010-07-24T06:46:08Z

Stathismar: Table edited by Stathismar via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|xanQ}}{{Tab|Gene|xanQ}}{{Tab On|Gene Product(s)|xanQ}}{{Tab|Expression|xanQ}}{{Tab|Evolution|xanQ}}{{Tab|On One Page|xanQ}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==

{{Product_nomenclature_help}}
{| id="H46aac44548aff" class=" tableEdit Product_nomenclature_table"

|-
!align=left {{table heading style}} |Standard name
||
XanQ
|-
!align=left {{table heading style}} |Synonyms
||
predicted transporter<ref name='LIB:Riley_2006'/>, B2882<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YgfO<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
YgfO NCS2 transporter<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Xanthine permease<ref name='LIB:EcoGene'/> <ref name='PMID:16096267'/>
|-
!align=left {{table heading style}} |EC number (for enzymes)
||

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.H46aac44548aff&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}


<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> 
{{Product_GO_help}}
{| id="I46aac44655e93" class=" tableEdit GO_table_product dataTable"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
GO:0005887
|
integral to plasma membrane
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0042906
|
xanthine transport
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0042907
|
xanthine transmembrane transporter activity
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0055085
|
transmembrane transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR006042
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0055085
|
transmembrane transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR006043
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.I46aac44655e93&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>

{{Product_interactions_help}}
{| id="W46aac44961f80" class=" tableEdit Product_interactions_table dataTable"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.W46aac44961f80&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || || || ||
|}


</protect>
===Notes===

==Localization==

{{Product_localization_help}}
{| id="O46aac44a29180" class=" tableEdit Product_localization_table"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Source!!Notes
|-
|
plasma membrane
|
C-terminus localized in the cytoplasm with 12 predicted transmembrane domains
|
Daley et al. (2005) <ref name=PMID:15919996/>
|

|

|-
|
plasma membrane
|

|

|

|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.O46aac44a29180&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table] || || || ||
|}


<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>

{{Product_physical_properties_help}}
{| id="N46aac44bbe1ce" class=" tableEdit Product_phys_prop_table"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=YGFO-MONOMER at EcoCyc]
<pre>M SDINHAGSDL IFELEDRPPF HQALVGAITH LLAIFVPMVT
PALIVGAALQ LSAETTAYLV SMAMIASGIG TWLQVNRYGI VGSGLLSIQS VNFSFVTVMI
ALGSSMKSDG FHEELIMSSL LGVSFVGAFL VVGSSFILPY LRRVITPTVS GIVVLMIGLS
LIKVGIIDFG GGFAAKSSGT FGNYEHLGVG LLVLIVVIGF NCCRSPLLRM GGIAIGLCVG
YIASLCLGMV DFSSMRNLPL ITIPHPFKYG FSFSFHQFLV VGTIYLLSVL EAVGDITATA
MVSRRPIQGE EYQSRLKGGV LADGLVSVIA SAVGSLPLTT FAQNNGVIQM TGVASRYVGR
TIAVMLVILG LFPMIGGFFT TIPSAVLGGA MTLMFSMIAI AGIRIIITNG LKRRETLIVA
TSLGLGLGVS YDPEIFKILP ASIYVLVENP ICAGGLTAIL LNIILPGGYR QENVLPGITS
AEEMD</pre>
|-
!align=left {{table heading style}} |Length
||
466
|-
!align=left {{table heading style}} |Mol. Wt
||
49.109 kDa
|-
!align=left {{table heading style}} |pI
||
6.9 (calculated)
|-
!align=left {{table heading style}} |Extinction coefficient
||
23,380 - 24,005 (calc based on 12 Y, 1 W, and 5 C residues)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.N46aac44bbe1ce&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_phys_prop_table edit table] ||
|}


<big>'''Domains/Motifs/Modification Sites'''</big>
<table>
<tr align="left" valign="top">
<td>


{{Product_domains_motifs_help}}
{| id="Q46aac44c3a551" class=" tableEdit Product_motif_table dataTable"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes!!References
|-
|
Domain
|
22..416
|
[http://pfam.janelia.org/family/PF00860 PF00860 Permease family]
|

|
PMID:19920124

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.Q46aac44c3a551&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table] || || || ||
|}


</td>
<td>


<motif_map/>
{{TMHMM Picture|ygfO}}
</td>
</tr>
</table>

<table><tr valign='top'><td>
<big>'''Structure'''</big> 

{{Product_structure_help}}
{| id="U46a85a1f3d659" class=" tableEdit Product_structure_table"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=ygfO
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.U46a85a1f3d659&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}


</td><td></protect>
==== Structure figures ====

<protect>
</td><tr><table></protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}

{{Product_resources_help}}
{| id="O46aac44d059a9" class=" tableEdit Product_resource_table"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.O46aac44d059a9&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table] || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="H46aac44d64a0c" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=YGFO-MONOMER EcoCyc:YGFO-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P67444|UniProt:P67444]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|

|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417358 RefSeq:NP_417358]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.H46aac44d64a0c&page=26127&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="G46a85a21ead26" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.G46a85a21ead26&page=26127&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0015292_!_uniporter activity]][[Category:Location:plasma membrane]]
[[Category:Pfam:PF00860_!_Permease family]]

xanQ:Gene Product(s)

2010-07-24T06:45:07Z

Stathismar: Table edited by Stathismar via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|xanQ}}{{Tab|Gene|xanQ}}{{Tab On|Gene Product(s)|xanQ}}{{Tab|Expression|xanQ}}{{Tab|Evolution|xanQ}}{{Tab|On One Page|xanQ}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==

{{Product_nomenclature_help}}
{| id="H46aac44548aff" class=" tableEdit Product_nomenclature_table"

|-
!align=left {{table heading style}} |Standard name
||
XanQ
|-
!align=left {{table heading style}} |Synonyms
||
predicted transporter<ref name='LIB:Riley_2006'/>, B2882<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YgfO<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
YgfO NCS2 tranporter<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Xanthine permease<ref name='LIB:EcoGene'/> <ref name='PMID:16096267'/>
|-
!align=left {{table heading style}} |EC number (for enzymes)
||

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.H46aac44548aff&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}


<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> 
{{Product_GO_help}}
{| id="I46aac44655e93" class=" tableEdit GO_table_product dataTable"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
GO:0005887
|
integral to plasma membrane
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0042906
|
xanthine transport
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0042907
|
xanthine transmembrane transporter activity
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0055085
|
transmembrane transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR006042
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0055085
|
transmembrane transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR006043
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.I46aac44655e93&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>

{{Product_interactions_help}}
{| id="W46aac44961f80" class=" tableEdit Product_interactions_table dataTable"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.W46aac44961f80&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || || || ||
|}


</protect>
===Notes===

==Localization==

{{Product_localization_help}}
{| id="O46aac44a29180" class=" tableEdit Product_localization_table"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Source!!Notes
|-
|
plasma membrane
|
C-terminus localized in the cytoplasm with 12 predicted transmembrane domains
|
Daley et al. (2005) <ref name=PMID:15919996/>
|

|

|-
|
plasma membrane
|

|

|

|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.O46aac44a29180&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table] || || || ||
|}


<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>

{{Product_physical_properties_help}}
{| id="N46aac44bbe1ce" class=" tableEdit Product_phys_prop_table"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=YGFO-MONOMER at EcoCyc]
<pre>M SDINHAGSDL IFELEDRPPF HQALVGAITH LLAIFVPMVT
PALIVGAALQ LSAETTAYLV SMAMIASGIG TWLQVNRYGI VGSGLLSIQS VNFSFVTVMI
ALGSSMKSDG FHEELIMSSL LGVSFVGAFL VVGSSFILPY LRRVITPTVS GIVVLMIGLS
LIKVGIIDFG GGFAAKSSGT FGNYEHLGVG LLVLIVVIGF NCCRSPLLRM GGIAIGLCVG
YIASLCLGMV DFSSMRNLPL ITIPHPFKYG FSFSFHQFLV VGTIYLLSVL EAVGDITATA
MVSRRPIQGE EYQSRLKGGV LADGLVSVIA SAVGSLPLTT FAQNNGVIQM TGVASRYVGR
TIAVMLVILG LFPMIGGFFT TIPSAVLGGA MTLMFSMIAI AGIRIIITNG LKRRETLIVA
TSLGLGLGVS YDPEIFKILP ASIYVLVENP ICAGGLTAIL LNIILPGGYR QENVLPGITS
AEEMD</pre>
|-
!align=left {{table heading style}} |Length
||
466
|-
!align=left {{table heading style}} |Mol. Wt
||
49.109 kDa
|-
!align=left {{table heading style}} |pI
||
6.9 (calculated)
|-
!align=left {{table heading style}} |Extinction coefficient
||
23,380 - 24,005 (calc based on 12 Y, 1 W, and 5 C residues)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.N46aac44bbe1ce&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_phys_prop_table edit table] ||
|}


<big>'''Domains/Motifs/Modification Sites'''</big>
<table>
<tr align="left" valign="top">
<td>


{{Product_domains_motifs_help}}
{| id="Q46aac44c3a551" class=" tableEdit Product_motif_table dataTable"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes!!References
|-
|
Domain
|
22..416
|
[http://pfam.janelia.org/family/PF00860 PF00860 Permease family]
|

|
PMID:19920124

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.Q46aac44c3a551&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table] || || || ||
|}


</td>
<td>


<motif_map/>
{{TMHMM Picture|ygfO}}
</td>
</tr>
</table>

<table><tr valign='top'><td>
<big>'''Structure'''</big> 

{{Product_structure_help}}
{| id="U46a85a1f3d659" class=" tableEdit Product_structure_table"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=ygfO
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.U46a85a1f3d659&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}


</td><td></protect>
==== Structure figures ====

<protect>
</td><tr><table></protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}

{{Product_resources_help}}
{| id="O46aac44d059a9" class=" tableEdit Product_resource_table"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.O46aac44d059a9&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table] || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="H46aac44d64a0c" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=YGFO-MONOMER EcoCyc:YGFO-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P67444|UniProt:P67444]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|

|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417358 RefSeq:NP_417358]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.H46aac44d64a0c&page=26127&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="G46a85a21ead26" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.G46a85a21ead26&page=26127&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0015292_!_uniporter activity]][[Category:Location:plasma membrane]]
[[Category:Pfam:PF00860_!_Permease family]]

xanQ:Gene Product(s)

2010-07-24T06:34:04Z

Stathismar: Table edited by Stathismar via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|xanQ}}{{Tab|Gene|xanQ}}{{Tab On|Gene Product(s)|xanQ}}{{Tab|Expression|xanQ}}{{Tab|Evolution|xanQ}}{{Tab|On One Page|xanQ}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==

{{Product_nomenclature_help}}
{| id="H46aac44548aff" class=" tableEdit Product_nomenclature_table"

|-
!align=left {{table heading style}} |Standard name
||
XanQ
|-
!align=left {{table heading style}} |Synonyms
||
predicted transporter<ref name='LIB:Riley_2006'/>, B2882<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YgfO<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
YgfO NCS2 tranporter<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Xanthine permease<ref name='LIB:EcoGene'/>
|-
!align=left {{table heading style}} |EC number (for enzymes)
||

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.H46aac44548aff&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}


<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> 
{{Product_GO_help}}
{| id="I46aac44655e93" class=" tableEdit GO_table_product dataTable"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
GO:0005887
|
integral to plasma membrane
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0042906
|
xanthine transport
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0042907
|
xanthine transmembrane transporter activity
|
PMID:16096267
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0055085
|
transmembrane transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR006042
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0055085
|
transmembrane transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR006043
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.I46aac44655e93&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>

{{Product_interactions_help}}
{| id="W46aac44961f80" class=" tableEdit Product_interactions_table dataTable"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.W46aac44961f80&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || || || ||
|}


</protect>
===Notes===

==Localization==

{{Product_localization_help}}
{| id="O46aac44a29180" class=" tableEdit Product_localization_table"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Source!!Notes
|-
|
plasma membrane
|
C-terminus localized in the cytoplasm with 12 predicted transmembrane domains
|
Daley et al. (2005) <ref name=PMID:15919996/>
|

|

|-
|
plasma membrane
|

|

|

|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.O46aac44a29180&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table] || || || ||
|}


<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>

{{Product_physical_properties_help}}
{| id="N46aac44bbe1ce" class=" tableEdit Product_phys_prop_table"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=YGFO-MONOMER at EcoCyc]
<pre>M SDINHAGSDL IFELEDRPPF HQALVGAITH LLAIFVPMVT
PALIVGAALQ LSAETTAYLV SMAMIASGIG TWLQVNRYGI VGSGLLSIQS VNFSFVTVMI
ALGSSMKSDG FHEELIMSSL LGVSFVGAFL VVGSSFILPY LRRVITPTVS GIVVLMIGLS
LIKVGIIDFG GGFAAKSSGT FGNYEHLGVG LLVLIVVIGF NCCRSPLLRM GGIAIGLCVG
YIASLCLGMV DFSSMRNLPL ITIPHPFKYG FSFSFHQFLV VGTIYLLSVL EAVGDITATA
MVSRRPIQGE EYQSRLKGGV LADGLVSVIA SAVGSLPLTT FAQNNGVIQM TGVASRYVGR
TIAVMLVILG LFPMIGGFFT TIPSAVLGGA MTLMFSMIAI AGIRIIITNG LKRRETLIVA
TSLGLGLGVS YDPEIFKILP ASIYVLVENP ICAGGLTAIL LNIILPGGYR QENVLPGITS
AEEMD</pre>
|-
!align=left {{table heading style}} |Length
||
466
|-
!align=left {{table heading style}} |Mol. Wt
||
49.109 kDa
|-
!align=left {{table heading style}} |pI
||
6.9 (calculated)
|-
!align=left {{table heading style}} |Extinction coefficient
||
23,380 - 24,005 (calc based on 12 Y, 1 W, and 5 C residues)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.N46aac44bbe1ce&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_phys_prop_table edit table] ||
|}


<big>'''Domains/Motifs/Modification Sites'''</big>
<table>
<tr align="left" valign="top">
<td>


{{Product_domains_motifs_help}}
{| id="Q46aac44c3a551" class=" tableEdit Product_motif_table dataTable"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes!!References
|-
|
Domain
|
22..416
|
[http://pfam.janelia.org/family/PF00860 PF00860 Permease family]
|

|
PMID:19920124

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.Q46aac44c3a551&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table] || || || ||
|}


</td>
<td>


<motif_map/>
{{TMHMM Picture|ygfO}}
</td>
</tr>
</table>

<table><tr valign='top'><td>
<big>'''Structure'''</big> 

{{Product_structure_help}}
{| id="U46a85a1f3d659" class=" tableEdit Product_structure_table"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=ygfO
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.U46a85a1f3d659&page=26127&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}


</td><td></protect>
==== Structure figures ====

<protect>
</td><tr><table></protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}

{{Product_resources_help}}
{| id="O46aac44d059a9" class=" tableEdit Product_resource_table"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.O46aac44d059a9&page=26127&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table] || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="H46aac44d64a0c" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=YGFO-MONOMER EcoCyc:YGFO-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P67444|UniProt:P67444]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|

|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417358 RefSeq:NP_417358]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.H46aac44d64a0c&page=26127&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
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{| id="G46a85a21ead26" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26127.G46a85a21ead26&page=26127&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude>
==References==
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{{RefHelp}}
<references />
__NOTOC__
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0015292_!_uniporter activity]][[Category:Location:plasma membrane]]
[[Category:Pfam:PF00860_!_Permease family]]

PMID:20406814

2010-07-24T06:17:30Z

Stathismar: /* Useful Materials and Methods {{HelpIcon|Help:Literature}} */

{{RightTOC}}
<protect>
 '''Georgopoulou, E, Mermelekas, G, Karena, E and Frillingos, S''' (2010) Purine substrate recognition by the nucleobase-ascorbate transporter signature motif in the YgfO xanthine permease: ASN-325 binds and ALA-323 senses substrate.''J. Biol. Chem.'' '''285''':19422-33

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The nucleobase-ascorbate transporter (NAT) signature motif is a conserved 11-amino acid sequence of the ubiquitous NAT/NCS2 family, essential for function and selectivity of both a bacterial (YgfO) and a fungal (UapA) purine-transporting homolog. We examined the role of NAT motif in more detail, using Cys-scanning and site-directed alkylation analysis of the YgfO xanthine permease of Escherichia coli. Analysis of single-Cys mutants in the sequence 315-339 for sensitivity to inactivation by 2-sulfonatoethyl methanethiosulfonate (MTSES(-)) and N-ethylmaleimide (NEM) showed a similar pattern: highly sensitive mutants clustering at the motif sequence (323-329) and a short alpha-helical face downstream (332, 333, 336). In the presence of substrate, N325C is protected from alkylation with either MTSES(-) or NEM, whereas sensitivity of A323C to inactivation by NEM is enhanced, shifting IC(50) from 34 to 14 microM. Alkylation or sensitivity of the other mutants is unaffected by substrate; the lack of an effect on Q324C is attributed to gross inability of this mutant for high affinity binding. Site-directed mutants G333R and S336N at the alpha-helical face downstream the motif display specific changes in ligand recognition relative to wild type; G333R allows binding of 7-methyl and 8-methylxanthine, whereas S336N disrupts affinity for 6-thioxanthine. Finally, all assayable motif-mutants are highly accessible to MTSES(-) from the periplasmic side. The data suggest that the NAT motif region lines the solvent- and substrate-accessible inner cavity, Asn-325 is at the binding site, Ala-323 responds to binding with a specific conformational shift, and Gly-333 and Ser-336 form part of the purine permeation pathway.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' ||
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20406814 PubMed]
Online version:[http://dx.doi.org/10.1074/jbc.M110.120543 10.1074/jbc.M110.120543]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
Demonstration that a conserved sequence motif of YgfO (XanQ) in at the transporter binding site, using a novel substituted-cysteine accessibility approach.

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}
Sulfhydryl alkylation assay to determine accessibility/reactivity of an engineered single-Cys in the presence or absence of substrate is performed with preincubation of RSO vesicles with reagent, at the appropriate conditions, followed by extraction of membrane proteins with DDM and isolation of His10-tagged permease through nickel-affinity chromatography, in-column denaturation and labeling with Oregon Green 488 maleimide.

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="G4c1c7af85eea1" class=" tableEdit GO_table_reference"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.172153.G4c1c7af85eea1&page=172153&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:20406814

2010-07-24T05:55:30Z

Stathismar: /* Significance {{HelpIcon|Help:Literature}} */

{{RightTOC}}
<protect>
 '''Georgopoulou, E, Mermelekas, G, Karena, E and Frillingos, S''' (2010) Purine substrate recognition by the nucleobase-ascorbate transporter signature motif in the YgfO xanthine permease: ASN-325 binds and ALA-323 senses substrate.''J. Biol. Chem.'' '''285''':19422-33

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The nucleobase-ascorbate transporter (NAT) signature motif is a conserved 11-amino acid sequence of the ubiquitous NAT/NCS2 family, essential for function and selectivity of both a bacterial (YgfO) and a fungal (UapA) purine-transporting homolog. We examined the role of NAT motif in more detail, using Cys-scanning and site-directed alkylation analysis of the YgfO xanthine permease of Escherichia coli. Analysis of single-Cys mutants in the sequence 315-339 for sensitivity to inactivation by 2-sulfonatoethyl methanethiosulfonate (MTSES(-)) and N-ethylmaleimide (NEM) showed a similar pattern: highly sensitive mutants clustering at the motif sequence (323-329) and a short alpha-helical face downstream (332, 333, 336). In the presence of substrate, N325C is protected from alkylation with either MTSES(-) or NEM, whereas sensitivity of A323C to inactivation by NEM is enhanced, shifting IC(50) from 34 to 14 microM. Alkylation or sensitivity of the other mutants is unaffected by substrate; the lack of an effect on Q324C is attributed to gross inability of this mutant for high affinity binding. Site-directed mutants G333R and S336N at the alpha-helical face downstream the motif display specific changes in ligand recognition relative to wild type; G333R allows binding of 7-methyl and 8-methylxanthine, whereas S336N disrupts affinity for 6-thioxanthine. Finally, all assayable motif-mutants are highly accessible to MTSES(-) from the periplasmic side. The data suggest that the NAT motif region lines the solvent- and substrate-accessible inner cavity, Asn-325 is at the binding site, Ala-323 responds to binding with a specific conformational shift, and Gly-333 and Ser-336 form part of the purine permeation pathway.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' ||
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20406814 PubMed]
Online version:[http://dx.doi.org/10.1074/jbc.M110.120543 10.1074/jbc.M110.120543]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
Demonstration that a conserved sequence motif of YgfO (XanQ) in at the transporter binding site, using a novel substituted-cysteine accessibility approach.

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="G4c1c7af85eea1" class=" tableEdit GO_table_reference"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.172153.G4c1c7af85eea1&page=172153&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:19581302

2010-07-24T05:50:35Z

Stathismar: /* Significance {{HelpIcon|Help:Literature}} */

{{RightTOC}}
<protect>
 '''Karena, E and Frillingos, S''' (2009) Role of intramembrane polar residues in the YgfO xanthine permease: HIS-31 and ASN-93 are crucial for affinity and specificity, and ASP-304 and GLU-272 are irreplaceable.''J. Biol. Chem.'' '''284''':24257-68

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Using the YgfO xanthine permease of Escherichia coli as a bacterial model for the study of the evolutionarily ubiquitous nucleobase-ascorbate transporter (NAT/NCS2) family, we performed a systematic Cys-scanning and site-directed mutagenesis of 14 putatively charged (Asp, Glu, His, Lys, or Arg) and 7 highly polar (Gln or Asn) residues that are predicted to lie in transmembrane helices (TMs). Of 21 single-Cys mutants engineered in the background of a functional YgfO devoid of Cys residues (C-less), only four are inactive or have marginal activity (H31C, N93C, E272C, D304C). The 4 residues are conserved throughout the family in TM1 (His-31), TM3 (Asn-93/Ser/Thr), TM8 (Glu-272), and putative TM9a (Asp-304/Asn/Glu). Extensive site-directed mutagenesis in wild-type background showed that H31N and H31Q have high activity and affinity for xanthine but H31Q recognizes novel purine bases and analogues, whereas H31C and H31L have impaired affinity for xanthine and analogues, and H31K or H31R impairs expression in the membrane. N93S and N93A are highly active but more promiscuous for recognition of analogues at the imidazole moiety of substrate, N93D has low activity, N93T has low affinity for xanthine or analogues, and N93Q or N93C is inactive. All mutants replacing Glu-272 or Asp-304, including E272D, E272Q, D304E, and D304N, are inactive, although expressed to high levels in the membrane. Finally, one of the 17 assayable single-Cys mutants, Q258C, was sensitive to inactivation by N-ethylmaleimide. The findings suggest that polar residues important for the function of YgfO cluster in TMs 1, 3, 8 and 9a.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' ||
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19581302 PubMed]
Online version:[http://dx.doi.org/10.1074/jbc.M109.030734 10.1074/jbc.M109.030734]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
A systematic mutagenesis of YgfO (XanQ) revealing four irreplaceable residues with conserved roles in the NAT/NCS2 family.

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="O4c244a41b1bee" class=" tableEdit GO_table_reference"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.172170.O4c244a41b1bee&page=172170&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:16096267

2010-07-01T06:44:47Z

Stathismar: /* Significance {{HelpIcon|Help:Literature}} */

{{RightTOC}}
<protect>
 '''Karatza, P and Frillingos, S''' Cloning and functional characterization of two bacterial members of the NAT/NCS2 family in Escherichia coli.''Mol. Membr. Biol.'' '''22''':251-61

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The coding potential of the genome of E. coli K-12 includes YgfO and YicE, two members of the evolutionarily conserved NAT/NCS2 transporter family that are highly homologous to each other (45% residue identity) and closely related to UapA of Aspergillus nidulans, a most extensively studied microbial member of this family. YgfO and yicE were cloned from the genome, over-expressed extrachromosomally and assayed for uptake of [(3)H]xanthine and other nucleobases, in E. coli K-12, under conditions of negligible activity of the corresponding endogenous systems. Alternative, essentially equivalent functional versions of YgfO and YicE were engineered by C-terminal tagging with an epitope from the E. coli lactose permease and a biotin-acceptor domain from Klebsiella pneumoniae. Both YgfO and YicE were shown to be present in the plasma membrane of E. coli and function as specific, high-affinity transporters for xanthine (K(m) 4.2-4.6 microM for YgfO, or 2.9-3.8 microM for YicE), in a proton motive force-dependent manner; they display no detectable transport of uracil, hypoxanthine, or uric acid at external concentrations of up to 0.1 mM. Both YgfO and YicE are inefficient in recognizing uric acid or xanthine analogues modified at position 8 of the purine ring (8-methylxanthine, 8-azaxanthine, oxypurinol, allopurinol), which distinguishes them from their fungal homologues UapA and Xut1.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' ||
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16096267 PubMed]
Online version:[http://dx.doi.org/10.1080/09687860500092927 10.1080/09687860500092927]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

The first demonstration that YgfO (XanQ) and YicE (XanP) of E. coli K-12 act as specific, high-affinity xanthine:proton symporters

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="L4c243afaec0d5" class=" tableEdit GO_table_reference"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.172168.L4c243afaec0d5&page=172168&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:17077086

2010-07-01T06:28:26Z

Stathismar: Fill PMID: Page!

{{RightTOC}}
<protect>
 '''Karatza, P, Panos, P, Georgopoulou, E and Frillingos, S''' (2006) Cysteine-scanning analysis of the nucleobase-ascorbate transporter signature motif in YgfO permease of Escherichia coli: Gln-324 and Asn-325 are essential, and Ile-329-Val-339 form an alpha-helix.''J. Biol. Chem.'' '''281''':39881-90

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The nucleobase-ascorbate transporter (NAT) signature motif is a conserved sequence motif of the ubiquitous NAT/NCS2 family implicated in defining the function and selectivity of purine translocation pathway in the major fungal homolog UapA. To analyze the role of NAT motif more systematically, we employed Cys-scanning mutagenesis of the Escherichia coli xanthine-specific homolog YgfO. Using a functional mutant devoid of Cys residues (C-less), each amino acid residue in sequence (315)GSIPITTFAQNNGVIQMTGVASRYVG(340) (motif underlined) was replaced individually with Cys. Of the 26 single-Cys mutants, 16 accumulate xanthine to > or =50% of the steady state observed with C-less YgfO, 4 accumulate to low levels (10-25% of C-less), F322C, N325C, and N326C accumulate marginally (5-8% of C-less), and P318C, Q324C, and G340C are inactive. When transferred to wild type, F322C(wt) and N326C(wt) are highly active, but P318G(wt), Q324C(wt), N325C(wt), and G340C(wt) are inactive, and G340A(wt) displays low activity. Immunoblot analysis shows that replacements at Pro-318 or Gly-340 are associated with low or negligible expression in the membrane. More extensive mutagenesis reveals that Gln-324 is critical for high affinity uptake and ligand recognition, and Asn-325 is irreplaceable for active xanthine transport, whereas Thr-332 and Gly-333 are important determinants of ligand specificity. All single-Cys mutants react with N-ethylmaleimide, but regarding sensitivity to inactivation, they fall to three regions; positions 315-322 are insensitive to N-ethylmaleimide, with IC(50) values > or =0.4 mM, positions 323-329 are highly sensitive, with IC(50) values of 15-80 microM, and sensitivity of positions 330-340 follows a periodicity, with mutants sensitive to inactivation clustering on one face of an alpha-helix.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' ||
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17077086 PubMed]
Online version:[http://dx.doi.org/10.1074/jbc.M605748200 10.1074/jbc.M605748200]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="Q4c2c3589cf55a" class=" tableEdit GO_table_reference"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.172227.Q4c2c3589cf55a&page=172227&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:17077086

2010-07-01T06:28:25Z

Stathismar: New PMID: Page!

PMID: 17077086

2010-07-01T06:28:25Z

Stathismar: New PMID: Page!

#REDIRECT [[PMID:17077086]]

PMID:18359771

2010-07-01T06:27:47Z

Stathismar: Fill PMID: Page!

{{RightTOC}}
<protect>
 '''Papakostas, K, Georgopoulou, E and Frillingos, S''' (2008) Cysteine-scanning analysis of putative helix XII in the YgfO xanthine permease: ILE-432 and ASN-430 are important.''J. Biol. Chem.'' '''283''':13666-78

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Transmembrane helix XII of UapA, the major fungal homolog of the nucleobase-ascorbate transporter (NAT/NCS2) family, has been proposed to contain an aromatic residue acting as a purine-selectivity filter, distinct from the binding site. To analyze the role of helix XII more systematically, we employed Cys-scanning mutagenesis of the Escherichia coli xanthine-specific homolog YgfO. Using a functional mutant devoid of Cys residues (C-less), each amino acid residue in sequence 419ILPASIYVLVENPICAGGLTAILLNIILPGGY450 (the putative helix XII is underlined) was replaced individually with Cys. Of the 32 single-Cys mutants, 25 accumulate xanthine to 80-130% of the steady state observed with C-less YgfO, six (P421C, S423C, I424C, Y425C, L427C, G436C) accumulate to low levels (15-40%), and I432C is inactive. Immunoblot analysis shows that P421C and I432C display low expression in the membrane. Extensive mutagenesis reveals that replacement of Ile-432 with equally or more bulky side chains abolishes active transport without affecting expression, whereas replacement with smaller side chains allows activity but impairs affinity for the analogues 1-methyl and 6-thioxanthine. Only three of the single-Cys mutants of helix XII (V426C, N430C, and N443C) are sensitive to inactivation by N-ethylmaleimide. N430C is highly sensitive, with an IC50 of 10 microm, and is completely protected against inactivation in the presence of 2-thioxanthine, a high affinity substrate analogue. Other xanthine analogues are poorly bound by N430C, whereas replacement of Asn-430 with Thr inactivates the permease. The findings suggest that Ile-432 and Asn-430 of helix XII are crucial for purine uptake and affinity, and Asn-430 is probably at the vicinity of the binding site.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' ||
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18359771 PubMed]
Online version:[http://dx.doi.org/10.1074/jbc.M800261200 10.1074/jbc.M800261200]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="Q4c2c35635fc3a" class=" tableEdit GO_table_reference"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.172225.Q4c2c35635fc3a&page=172225&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:18359771

2010-07-01T06:27:47Z

Stathismar: New PMID: Page!

PMID: 18359771

2010-07-01T06:27:46Z

Stathismar: New PMID: Page!

#REDIRECT [[PMID:18359771]]

PMID:19581302

2010-06-25T06:18:44Z

Stathismar: Fill PMID: Page!

{{RightTOC}}
<protect>
 '''Karena, E and Frillingos, S''' (2009) Role of intramembrane polar residues in the YgfO xanthine permease: HIS-31 and ASN-93 are crucial for affinity and specificity, and ASP-304 and GLU-272 are irreplaceable.''J. Biol. Chem.'' '''284''':24257-68

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Using the YgfO xanthine permease of Escherichia coli as a bacterial model for the study of the evolutionarily ubiquitous nucleobase-ascorbate transporter (NAT/NCS2) family, we performed a systematic Cys-scanning and site-directed mutagenesis of 14 putatively charged (Asp, Glu, His, Lys, or Arg) and 7 highly polar (Gln or Asn) residues that are predicted to lie in transmembrane helices (TMs). Of 21 single-Cys mutants engineered in the background of a functional YgfO devoid of Cys residues (C-less), only four are inactive or have marginal activity (H31C, N93C, E272C, D304C). The 4 residues are conserved throughout the family in TM1 (His-31), TM3 (Asn-93/Ser/Thr), TM8 (Glu-272), and putative TM9a (Asp-304/Asn/Glu). Extensive site-directed mutagenesis in wild-type background showed that H31N and H31Q have high activity and affinity for xanthine but H31Q recognizes novel purine bases and analogues, whereas H31C and H31L have impaired affinity for xanthine and analogues, and H31K or H31R impairs expression in the membrane. N93S and N93A are highly active but more promiscuous for recognition of analogues at the imidazole moiety of substrate, N93D has low activity, N93T has low affinity for xanthine or analogues, and N93Q or N93C is inactive. All mutants replacing Glu-272 or Asp-304, including E272D, E272Q, D304E, and D304N, are inactive, although expressed to high levels in the membrane. Finally, one of the 17 assayable single-Cys mutants, Q258C, was sensitive to inactivation by N-ethylmaleimide. The findings suggest that polar residues important for the function of YgfO cluster in TMs 1, 3, 8 and 9a.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' ||
* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19581302 PubMed]
Online version:[http://dx.doi.org/10.1074/jbc.M109.030734 10.1074/jbc.M109.030734]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="O4c244a41b1bee" class=" tableEdit GO_table_reference"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.172170.O4c244a41b1bee&page=172170&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:19581302

2010-06-25T06:18:41Z

Stathismar: New PMID: Page!

PMID: 19581302

2010-06-25T06:18:36Z

Stathismar: New PMID: Page!

#REDIRECT [[PMID:19581302]]