PMID:11983857

2007-10-11T16:51:41Z

Parkinson: New PMID: Page!

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<span style = 'font-size: large'> '''Ames, P, Studdert, CA, Reiser, RH and Parkinson, JS''' (2002) Collaborative signaling by mixed chemoreceptor teams in Escherichia coli.''Proc. Natl. Acad. Sci. U.S.A.'' '''99''':7060-5</span>

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|{{TableHeadingColor}} |'''Abstract''' || Chemoreceptors of the methyl-accepting chemotaxis protein family form clusters, typically at the cell pole(s), in both Bacteria and Archaea. To elucidate the architecture and signaling role of receptor clusters, we investigated interactions between the serine (Tsr) and aspartate (Tar) chemoreceptors in Escherichia coli by constructing Tsr mutations at the six hydrophobic and five polar residues implicated in "trimer of dimers" formation. Tsr mutants with proline replacements could not mediate serine chemotaxis, receptor clustering, or clockwise flagellar rotation. Alanine and tryptophan mutants, although also nonchemotactic, formed receptor clusters, and some produced clockwise flagellar rotation, indicating receptor-coupled activation of the signaling CheA kinase. The alanine and tryptophan mutants evidently assemble defective receptor complexes that cannot modulate CheA activity in response to serine stimuli. In cells containing wild-type Tar receptors, tryptophan replacements in Tsr interfered with Tar function, whereas four Tsr mutants with alanine replacements regained Tsr function. These epistatic and rescuable phenotypes imply interactions between Tsr and Tar dimers in higher-order signaling teams. The bulky side chain in tryptophan mutants may prevent stimulus-induced conformational changes in the team, whereas the small side chain in alanine mutants may permit signaling control when teamed with functional receptor molecules. Direct physical interactions between Tsr and Tar molecules were observed by in vivo chemical crosslinking. Wild-type Tsr crosslinked to Tar, whereas a clustering-defective proline replacement mutant did not. These findings indicate that bacterial chemoreceptor clusters are comprised of signaling teams, seemingly based on trimers of dimers, that can contain different receptor types acting collaboratively.
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11983857 PubMed]
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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
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{|class = 'sortable' {{Prettytable}} id='220883'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
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<references/>

tsr:Gene

2007-10-11T16:50:30Z

Parkinson: edited by Parkinson via TableEdit

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<noinclude>{{TabsTop}}
{{Tab|Quickview|tsr}}{{Tab On|Gene|tsr}}{{Tab|Gene Product(s)|tsr}}{{Tab|Expression|tsr}}{{Tab|Evolution|tsr}}{{Tab|On One Page|tsr}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
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==Nomenclature==
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{|{{Prettytable}}
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!{{table_heading_style}} |Standard name
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''tsr''
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!{{table_heading_style}} |Mnemonic
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!{{table_heading_style}} |Synonyms
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''ECK4345, b4355, JW4318, cheD''<ref name='LIB:Riley_2006'/>
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===Notes===

==Location(s) and DNA Sequence==
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{|class = 'sortable' {{Prettytable}} id='150994'
|- {{table_heading_style}}
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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MG1655
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98.92 minutes 
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MG1655: 4589680..4591335<br />{{GBrowseFigureByCoord|4588680|4592335|ecoli|MG1655}}
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*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11034 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:4588680..4592335 GBrowse(MG1655)]

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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:4589680..4591335&plugin_action=Go FASTA Dump]
*[http://biocyc.org/ECOLI/sequence?object=EG11034 EcoCyc Sequence Dump]
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W3110
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W3110: 4596337..4597992<br />{{GBrowseFigureByCoord|4595337|4598992|ecoli_W3110|W3110}}
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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:4595337..4598992 GBrowse(W3110)]
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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:4596337..4597992&plugin_action=Go FASTA Dump]
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===Notes===

==Sequence Features==
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{|class = 'sortable' {{Prettytable}} id='150995'
|- {{table_heading_style}}
!Feature type!!Location!!Description
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====Notes====

==Alleles and Phenotypes==
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{|class = 'sortable' {{Prettytable}} id='150996'
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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Δtsr (Keio:JW4318)
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deletion
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deletion
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[[PMID:16738554]]<ref name=PMID:16738554/>
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
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N381D
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N381
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Other
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null. No chemotaxis to serine
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I377P
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I377P
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Other
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null. No chemotaxis to serine
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[[PMID:11983857]]<ref name=PMID:11983857/>
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===Notes===

==Genetic Resources==
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{|class = 'sortable' {{Prettytable}} id='150997'
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!Resource!!Resource Type!!Source!!Notes/Reference
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JW4318
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Plasmid clone
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
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[[PMID:16769691]]<ref name=PMID:16769691/>

Status:Clone OK

Primer 1:GCCTTAAAACGTATCAAAATTGT

Primer 2:CCAAATGTTTCCCAGTTCTCCTC
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[[ytfN:Gene|zjf-920::Tn10]]
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Linked marker
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CAG12019 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40608 CGSC7484]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>

Synonyms:zjh-901::Tn10, zjg-920::Tn10
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[[mdoB:Gene|mdoB202::Tn10]]
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Linked marker
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CAG18430 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40618 CGSC7488]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 89% <ref name='LIB:Nichols_linked_Tn'/>

Synonyms:zjj-202::Tn10, zji-202::Tn10
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===Notes===

==Accessions in Other Databases==
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{|class = 'sortable' {{Prettytable}} id='150998'
|- {{table_heading_style}}
!Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11034 EcoCyc:EG11034]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1027 EchoBASE:EB1027]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b4355 EcoliGenExpDB:b4355]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C5430 ECOL199310CYC:C5430]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG11034 EcoGene:EG11034]
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|-
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=TSR ECOO157CYC:TSR]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/63 CGSC:63]
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{{Gene_accessions_help}}
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===Notes===

==Links==
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{|class = 'sortable' {{Prettytable}} id='100451'
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!Name!!URL!!Comments
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|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19261.Q46a725ae0c7de&page=19261&pagename={{FULLPAGENAMEE}} edit table] || ||
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{{Links_help}}

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==References==
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{{RefHelp}}
<references/>
__NOTOC__
</protect></noinclude>
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]

EcoliWiki - User contributions [en]

PMID:11983857

tsr:Gene