EcoliWiki - User contributions [en]

PMID:6314091

2008-05-01T07:37:16Z

Mattnat08: New PMID: Page!

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 '''Boos, W, Bantlow, C, Benner, D and Roller, E''' (1983) cir, a gene conferring resistance to colicin I maps between mgl and fpk on the Escherichia coli chromosome.''Mol. Gen. Genet.'' '''191''':401-6

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|{{TableHeadingColor}} |'''Abstract''' || <protect>With the help of the tetracycline resistance transposon Tn10 in and around the mgl genes the gene cir was mapped. cir is 80% cotransducible with mgl by P1 transduction. The sequence of the surrounding markers in clockwise order was established as: cdd fpk cir mgl gyrA. The direction of transcription in cir was determined as clockwise on the Escherichia coli chromosome. The gene product of cir, an outer membrane receptor for colicin I, is not part of the mgl operon. It is not regulated by D-fucose, the inducer of the mgl system and mutants defective in cir are unimpaired in the uptake of substrates of the mgl-dependent transport system.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6314091 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241935'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.K4819732b7dd00&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:2217198

2008-05-01T07:36:38Z

Mattnat08: New PMID: Page!

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 '''Bonner, CA, Hays, S, McEntee, K and Goodman, MF''' (1990) DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli.''Proc. Natl. Acad. Sci. U.S.A.'' '''87''':7663-7

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The structural gene for DNA polymerase II was cloned by using a synthetic inosine-containing oligonucleotide probe corresponding to 11 amino acids, which were determined by sequencing the amino terminus of the purified protein. The labeled oligonucleotide hybridized specifically to the lambda clone 7H9 from the Kohara collection as well as to plasmid pGW511 containing the SOS-regulated dinA gene. Approximately 1400 base pairs of dinA sequence were determined. The predicted amino-terminal sequence of dinA demonstrated that this gene encoded DNA polymerase II. Sequence analysis of the upstream region localized a LexA binding site overlapping the -35 region of the dinA promoter, and this promoter element was found to be only two nucleotides downstream from the 3' end of the araD gene. These results demonstrate that the gene order is thr-dinA (pol II)-ara-leu on the Escherichia coli chromosome and that the DNA polymerase II structural gene is transcribed in the same direction as the araBAD operon. Based on the analysis of the predicted protein, we have identified a sequence motif Asp-Xaa-Xaa-Ser-Leu-Tyr-Pro-Ser in DNA polymerase II that is highly conserved among a diverse group of DNA polymerases, which include those from humans, yeast, Herpes and vaccinia viruses, and phages T4 and PRD1. The demonstration that DNA polymerase II is a component of the SOS response in E. coli suggests that it plays an important role in DNA repair and/or mutagenesis.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2217198 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241934'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J4819730622ac5&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:7022129

2008-05-01T07:36:22Z

Mattnat08: New PMID: Page!

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 '''Bonnefoy-Orth, V, Lepelletier, M, Pascal, MC and Chippaux, M''' (1981) Nitrate reductase and cytochrome bnitrate reductase structural genes as parts of the nitrate reductase operon.''Mol. Gen. Genet.'' '''181''':535-40

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The existence of a nitrate-reductase operon in the tryptophane region was deduced from the effects of prophage insertion in each of chlI and chlC genes and from transposition of the Mu-mediated host DNA fragments of F-prime. This operon appears to be polarized from chlC to chlI and the gene order in the region is trp -- chlI -- chlC -- purB.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7022129 PubMed]
|}

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<protect>

{|class = 'sortable' {{Prettytable}} id='241933'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.I481972f56bb31&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:3329696

2008-05-01T07:36:06Z

Mattnat08: New PMID: Page!

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 '''Bonnefoy, V, Burini, JF, Giordano, G, Pascal, MC and Chippaux, M''' (1987) Presence in the 'silent' terminus region of the Escherichia coli K12 chromosome of cryptic gene(s) encoding a new nitrate reductase.''Mol. Microbiol.'' '''1''':143-50

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|{{TableHeadingColor}} |'''Abstract''' || <protect>A cosmid complementing narG mutants defective in nitrate reductase activity was isolated from a genomic library of Escherichia coli. The restriction map of the insert differed from that of the narGHI operon. The new enzyme, termed NarZ, required molybdenum for activity. The expression of narZ was not affected by the factors controlling narGHI. Insertion mutations indicated that the narZ locus covered about 8 kb of DNA; narZ is located at 32.5 U on the chromosome, in the cotransduction gap near the replication terminus. Southern blot experiments under stringent conditions using narGHI or narZ DNA as probes revealed a large extent of homology, with a small area of very high homology. We propose that narZ and narGHI have descended from a common ancestor by gene duplication.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3329696 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241932'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.A481972e5cc580&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:1425583

2008-05-01T07:35:37Z

Mattnat08: New PMID: Page!

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 '''Bonnefoy, E and Rouvière-Yaniv, J''' (1992) HU, the major histone-like protein of E. coli, modulates the binding of IHF to oriC.''EMBO J.'' '''11''':4489-96

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|{{TableHeadingColor}} |'''Abstract''' || <protect>HU is one of the most abundant DNA binding proteins of bacteria. Unlike IHF, integration host factor of Escherichia coli, with which HU shares many properties, including a strong sequence homology and similar predicted structure, HU seems to bind non-specifically to DNA whereas IHF binds to specific sites. In this work we compare the binding characteristics of HU and IHF to a DNA fragment containing the minimal origin of replication of E. coli (oriC) and we analyse the effect of HU on the binding capacity of IHF to this oriC fragment. We show that HU interacts randomly and non-specifically with oriC as opposed to the specific binding of IHF to this same DNA sequence. In addition, we show that HU can modulate the binding of IHF to its specific oriC site. Depending on the relative concentrations of HU and IHF, HU is able either to activate or to inhibit the binding of IHF to oriC.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1425583 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241931'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.G481972c85ee3d&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:7655112

2008-05-01T07:35:09Z

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 '''Murphy, SC, Lowitt, MH and Kao, GF''' (1995) Multiple eruptive dermatofibromas in an HIV-positive man.''Dermatology (Basel)'' '''190''':309-12

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|{{TableHeadingColor}} |'''Abstract''' || <protect>Multiple dermatofibromas (DFs) may occur in association with altered immunity, including systemic lupus erythematosus and iatrogenic immunosuppression. We report a case of multiple eruptive DFs which occurred in a patient positive for human immunodeficiency virus (HIV). The association of eruptive DFs and HIV infection has not been previously reported. The mechanism for the development of DFs in the setting of immune disturbance remains unclear. In the setting of HIV infection, DFs may clinically mimic Kaposi's sarcoma.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7655112 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241930'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.E481972acdd59e&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:161328

2008-05-01T07:34:18Z

Mattnat08: New PMID: Page!

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 '''Bollen, A, Lathe, R, Herzog, A, Denicourt, D, Lecocq, JP, Desmarez, L and Lavallé, R''' (1979) A conditionally lethal mutation of Escherichia coli affecting the gene coding for ribosomal protein S2 (rpsB).''J. Mol. Biol.'' '''132''':219-33

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|{{TableHeadingColor}} |'''Abstract''' || <protect>No abstract in PubMed</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=161328 PubMed]
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<protect>

{|class = 'sortable' {{Prettytable}} id='241929'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Q48197279f0c25&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:6285149

2008-05-01T07:33:29Z

Mattnat08: New PMID: Page!

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 '''Boidol, W, Simonis, M, Töpert, M and Siewert, G''' (1982) Recombinant plasmids with genes for the biosynthesis of alkaline phosphatase of Escherichia coli.''Mol. Gen. Genet.'' '''185''':510-2

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|{{TableHeadingColor}} |'''Abstract''' || <protect>Restriction maps of several recombinant plasmids representing a section of the E. coli K12 chromosome 35,000 bp in size with the genes phoA, proC and phoB were prepared. The orientation of phoA and the exact position of its N-terminal end on this map were determined by identifying a subfragment which carried the phoA promoter and by determining the nucleotide sequence of a 160 bp portion of this subfragment comprising the codons for the N-terminal end of pre-alkaline phosphatase. From this DNA sequence the leader sequence of alkaline phosphatase which consists of 21 amino acids was derived.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6285149 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241928'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.D48197248f0647&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:6991867

2008-05-01T07:33:11Z

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 '''Bohman, K and Isaksson, LA''' (1980) A temperature-sensitive mutant in prolinyl-tRNA ligase of Escherichia coli K-12.''Mol. Gen. Genet.'' '''177''':603-5

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|{{TableHeadingColor}} |'''Abstract''' || <protect>A mutant with a defective prolinyl-tRNA ligase has been found in a collection of spontaneous temperature-sensitive mutants. The mutated gene, which is designated proS, is closely linked to metD.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6991867 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241927'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.U4819723734563&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:396450

2008-05-01T07:32:57Z

Mattnat08: New PMID: Page!

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 '''Bohman, K and Isaksson, LA''' (1979) Temperature-sensitive mutants in cysteinyl-tRNA ligase of E. coli K-12.''Mol. Gen. Genet.'' '''176''':53-5

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|{{TableHeadingColor}} |'''Abstract''' || <protect>Two mutants with a defective cysteinyl-tRNA synthetase have been found in a collection of spontaneous temperature sensitive mutants. The mutated gene, which is designated cysS, is closely cotransduced with purE.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=396450 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241926'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.F4819722955e00&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:9211334

2008-05-01T07:32:05Z

Mattnat08: New PMID: Page!

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 '''Bacher, A, Eberhardt, S, Fischer, M, Mörtl, S, Kis, K, Kugelbrey, K, Scheuring, J and Schott, K''' (1997) Biosynthesis of riboflavin: lumazine synthase and riboflavin synthase.''Meth. Enzymol.'' '''280''':389-99

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|{{TableHeadingColor}} |'''Abstract''' || <protect>No abstract in PubMed</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9211334 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241925'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.E481971f501cc4&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:7557316

2008-05-01T07:31:42Z

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 '''Baccigalupi, L, Marasco, R, Ricca, E, De Felice, M and Sacco, M''' (1995) Control of ilvIH transcription during amino acid downshift in stringent and relaxed strains of Escherichia coli.''FEMS Microbiol. Lett.'' '''131''':95-8

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|{{TableHeadingColor}} |'''Abstract''' || <protect>Transcription of the ilvIH operon was reduced during amino acid starvation of wild-type Escherichia coli. The effect was abolished by a relA mutation and was enhanced by a spoT mutation, thus suggesting a possible negative role of ppGpp accumulation on ilvIH transcription. No effect of amino acid downshift was observed on the synthesis of lrp mRNA, encoding the positive regulator (Lrp) of ilvIH transcription.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7557316 PubMed]
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{|class = 'sortable' {{Prettytable}} id='241924'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.A481971de3a201&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:1846032

2008-05-01T07:31:24Z

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 '''Babitzke, P and Kushner, SR''' (1991) The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli.''Proc. Natl. Acad. Sci. U.S.A.'' '''88''':1-5

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|{{TableHeadingColor}} |'''Abstract''' || <protect>The in vitro and in vivo analysis of the ribonuclease E-deficient (rne-) and the altered mRNA stability protein-deficient (ams-) strains of Escherichia coli has demonstrated that they carry mutations in the same structural gene. Strains encoding either thermolabile RNase E (rne-3071) or Ams protein (ams-1) are defective in both rRNA processing and mRNA turnover. Immediately after a shift to the nonpermissive temperature, the chemical decay rate of bulk mRNA is slowed 2- to 3-fold, and within 70 min, precursors to 5S rRNA begin to accumulate. In addition, all of the phenotypes associated with either the rne-3071 or the ams-1 alleles were complemented by a recombinant plasmid carrying ams+. When taken together with previous genetic studies, these results suggest that the role of ribonuclease E in mRNA turnover involves endonucleolytic cleavages at the proposed ACAG(A/U)AUUUG consensus sequence.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1846032 PubMed]
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<protect>

{|class = 'sortable' {{Prettytable}} id='241923'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.P481971cbcab7d&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:7855594

2008-05-01T07:31:05Z

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 '''Babbitt, PC, Mrachko, GT, Hasson, MS, Huisman, GW, Kolter, R, Ringe, D, Petsko, GA, Kenyon, GL and Gerlt, JA''' (1995) A functionally diverse enzyme superfamily that abstracts the alpha protons of carboxylic acids.''Science'' '''267''':1159-61

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|{{TableHeadingColor}} |'''Abstract''' || <protect>Mandelate racemase and muconate lactonizing enzyme are structurally homologous but catalyze different reactions, each initiated by proton abstraction from carbon. The structural similarity to mandelate racemase of a previously unidentified gene product was used to deduce its function as a galactonate dehydratase. In this enzyme superfamily that has evolved to catalyze proton abstraction from carbon, three variations of homologous active site architectures are now represented: lysine and histidine bases in the active site of mandelate racemase, only a lysine base in the active site of muconate lactonizing enzyme, and only a histidine base in the active site of galactonate dehydratase. This discovery supports the hypothesis that new enzymatic activities evolve by recruitment of a protein catalyzing the same type of chemical reaction.</protect>
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7855594 PubMed]
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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241922'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Q481971b93c29b&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:8183945

2008-05-01T07:30:24Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Baba, T, Taura, T, Shimoike, T, Akiyama, Y, Yoshihisa, T and Ito, K''' (1994) A cytoplasmic domain is important for the formation of a SecY-SecE translocator complex.''Proc. Natl. Acad. Sci. U.S.A.'' '''91''':4539-43

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>An approach to identifying the interaction site of multicomponent protein assembly has been applied to the membrane-bound SecY-SecE complex, which mediates protein export across the Escherichia coli cytoplasmic membrane. A dominant negative secY allele, secY-d1, inactivates SecY but preserves its ability to interact with SecE. Thus, the mutant protein sequesters SecE in an inactive complex. Second site mutations that disrupt the SecE binding site will suppress the export interference. We introduced insertion/deletion mutations that intragenically suppressed secY-d1. After eliminating knock-out mutations by virtue of the expression of a LacZ alpha sequence that had been attached to the C terminus, we obtained a striking clustering of mutations in cytoplasmic domain 4. On the basis of this result, the secY24 (Ts) substitution mutation in this domain was examined for its effects on interaction with SecE. It indeed suppressed secY-d1. Although the instability associated with excess SecY can be alleviated by overproduction of SecE, the secY24 mutant protein was not stabilized by SecE. The basal-level SecY24 protein was also destabilized at 42 degrees C. SecE was coimmunoprecipitated with SecY+ but not with the SecY24 protein. These results indicate that the secY24 mutation weakens SecY's interaction with SecE. Taken together, we propose that cytoplasmic domain 4 is important for the association between SecY and SecE.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8183945 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241921'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.P4819718fbef97&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:2254269

2008-05-01T07:30:07Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Baba, T, Jacq, A, Brickman, E, Beckwith, J, Taura, T, Ueguchi, C, Akiyama, Y and Ito, K''' (1990) Characterization of cold-sensitive secY mutants of Escherichia coli.''J. Bacteriol.'' '''172''':7005-10

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Mutations which cause poor growth at a low temperature, which affect aspects of protein secretion, and which map in or around secY (prlA) were characterized. The prlA1012 mutant, previously shown to suppress a secA mutation, proved to have a wild-type secY gene, indicating that this mutation cannot be taken as genetic evidence for the secA-secY interaction. Two cold-sensitive mutants, the secY39 and secY40 mutants, which had been selected by their ability to enhance secA expression, contained single-amino-acid alterations in the same cytoplasmic domain of the SecY protein. Protein export in vivo was partially slowed down by the secY39 mutation at 37 to 39 degrees C, and the retardation was immediately and strikingly enhanced upon exposure to nonpermissive temperatures (15 to 23 degrees C). The rate of posttranslational translocation of the precursor to the OmpA protein (pro-OmpA protein) into wild-type membrane vesicles in vitro was only slightly affected by reaction temperatures ranging from 37 to 15 degrees C, and about 65% of OmpA was eventually sequestered at both temperatures. Membrane vesicles from the secY39 mutant were much less active in supporting pro-OmpA translocation even at 37 degrees C, at which about 20% sequestration was attained. At 15 degrees C, the activity of the mutant membrane decreased further. The rapid temperature response in vivo and the impaired in vitro translocation activity at low temperatures with the secY39 mutant support the notion that SecY, a membrane-embedded secretion factor, participates in protein translocation across the bacterial cytoplasmic membrane.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2254269 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241920'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R4819717eeb42d&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:7961418

2008-05-01T07:29:49Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Azizan, A and Black, PN''' (1994) Use of transposon TnphoA to identify genes for cell envelope proteins of Escherichia coli required for long-chain fatty acid transport: the periplasmic protein Tsp potentiates long-chain fatty acid transport.''J. Bacteriol.'' '''176''':6653-62

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>TnphoA was used to mutagenize the chromosome in an effort to identify membrane-bound and exported components of the long-chain fatty acid transport system of Escherichia coli. This strategy identified three classes of fusions that were unable to grow or grew at reduced rates on minimal agar plates containing the long-chain fatty acid oleate (C18:1), (i) fadL-phoA, (ii) tolC-phoA, and (iii) tsp-phoA, fadL-phoA and tolC-phoA fusions were unable to grow on oleate as the sole carbon and energy source, while the tsp-phoA fusion had a markedly reduced growth rate. As expected, fadL-phoA fusions were unable to grow on oleate plates because the outer membrane-bound fatty acid transport protein FadL was defective. The identification of multiple fadL-phoa fusions demonstrated that this strategy of mutagenesis specifically targeted membrane-bound and exported components required for growth on long-chain fatty acids. tolC-phoA fusions were sensitive to fatty acids (particularly medium chain) and thus unable to grow, whereas the reduced growth rate of tsp-phoA fusions on oleate was apparently due to changes in the energized state of the outer membrane or inner membrane. tsp-phoA fusions transported the long-chain fatty acid oleate at only 50% of wild-type levels when cells were energized with 1 mM DL-lactate. Under conditions in which transport was measured in the absence of lactate, tsp-phoA fusion strains and wild-type strains had the same levels of oleate transport. The tsp+ clone pAZA500 was able to restore wild-type transport activity to the tsp-phoA strain under lactate-energized conditions. These results indicate that the periplasmic protein Tsp potentiates long-chain fatty acid transport.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7961418 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241919'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.R4819716d2bb94&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:2656630

2008-04-21T20:49:35Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Avissar, YJ and Beale, SI''' (1989) Identification of the enzymatic basis for delta-aminolevulinic acid auxotrophy in a hemA mutant of Escherichia coli.''J. Bacteriol.'' '''171''':2919-24

{|{{Prettytable}} align = 'center' width= '70%'
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|{{TableHeadingColor}} |'''Abstract''' || <protect>The hemA mutation of Escherichia coli K-12 confers a requirement for delta-aminolevulinic acid (ALA). Cell extract prepared from the hemA strain SASX41B was incapable of producing ALA from either glutamate or glutamyl-tRNA, whereas extract of the hem+ strain HB101 formed colorimetrically detectable amounts of ALA and transferred label from 1-[14C]glutamate and 3,4-[3H]glutamyl-tRNA to ALA. Extracts of both strains converted glutamate-1-semialdehyde to ALA and were capable of aminoacylating tRNAGlu. Glutamyl-tRNA formed by extracts of both strains could be converted to ALA by the extract of hem+ cells. The extract of hemA cells did not convert glutamyl-tRNA formed by either strain to ALA. However, the hemA cell extract, when supplemented in vitro with glutamyl-tRNA dehydrogenase isolated from Chlorella vulgaris cells, formed about as much ALA as did the unsupplemented hem+ cell extract. We conclude from these observations that the enzyme activity that is lacking in the ALA auxotrophic strain carrying the hemA mutation is that of glutamyl-tRNA dehydrogenase.</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2656630 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241713'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.T480cfdddcbcbc&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:4915864

2008-04-21T20:48:24Z

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{{RightTOC}}

 '''Austin, S and Scaife, J''' (1970) A new method for selecting RNA polymerase mutants.''J. Mol. Biol.'' '''49''':263-7

{|{{Prettytable}} align = 'center' width= '70%'
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|{{TableHeadingColor}} |'''Abstract''' || <protect>No abstract in PubMed</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4915864 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241712'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.E480cfd9642f74&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:776928

2008-04-21T20:48:05Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Austin, S''' (1976) Wild-type and mutant in vitro products of an operon for ribonucleic acid polymerase subunits.''J. Bacteriol.'' '''127''':32-9

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>An in vitro protein-synthesizing system can synthesize two ribonucleic acid (RNA) polymerase subunits of Escherichia coli, beta and beta', when a transducing phage deoxyribonucleic acid (DNA) template containing the rpoB region of the bacterial chromosome is added. Recombinant rpoB transducing phages were isolated that carry "nonsense" mutations of the class rpo-rifampin zero amber (formally referred to as rifoam). DNA was extracted from two of these phages. These DNAs are unable to direct the synthesis of beta subunits, whereas beta' synthesis is unaffected. Both mutations can be efficiently suppressed in vitro by the addition of suppressor transfer RNA. One of the mutations (rpoB115) produces a detectable nonsense (or restart) fragment of the beta protein in the absence of suppression. It is concluded that rpoB115 is an amber mutation within the structural gene for the beta subunit of RNA polymerase.</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=776928 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241711'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B480cfd84d3324&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:2168379

2008-04-21T20:47:44Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Austin, EA, Graves, JF, Hite, LA, Parker, CT and Schnaitman, CA''' (1990) Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus.''J. Bacteriol.'' '''172''':5312-25

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Tn10 insertions were selected on the basis of resistance to the lipopolysaccharide (LPS)-specific bacteriophage U3. The majority of these were located in a 2-kilobase region within the rfa locus, a gene cluster of about 18 kb that contains genes for LPS core biosynthesis. The rfa::Tn10 insertions all exhibited a deep rough phenotype that included hypersensitivity to hydrophobic antibiotics, a reduction in major outer membrane proteins, and production of truncated LPS. These mutations were complemented by a Clarke-Carbon plasmid known to complement rfa mutations of Salmonella typhimurium, and analysis of the insert from this plasmid showed that it contained genes for at least six polypeptides which appear to be arranged in the form of a complex operon. Defects in two of these genes were specifically implicated as the cause of the deep rough phenotype. One of these appeared to be rfaG, which encodes a function required for attachment of the first glucose residue to the heptose region of the core. The other gene did not appear to be directly involved in determination of the sugar composition of the core. We speculate that the product of this gene is involved in the attachment of phosphate or phosphorylethanolamine to the core and that it is the lack of one of these substituents which results in the deep rough phenotype.</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2168379 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241710'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X480cfd6f6dc37&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:2527331

2008-04-21T20:46:37Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Auger, EA, Redding, KE, Plumb, T, Childs, LC, Meng, SY and Bennett, GN''' (1989) Construction of lac fusions to the inducible arginine- and lysine decarboxylase genes of Escherichia coli K12.''Mol. Microbiol.'' '''3''':609-20

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The induction of several amino acid decarboxylases under anaerobic conditions at low pH has been known for many years, but the mechanism associated with this type of regulation has not been elucidated. To study the regulation of the biodegradative arginine and lysine decarboxylases of Escherichia coli K12, Mudlac fusions to these genes were isolated. Mudlac fusion strains deficient for lysine decarboxylase or arginine decarboxylase were identified using decarboxylase indicator media and analysed for their regulation of beta-galactosidase expression. The position of the Mudlac fusion in lysine decarboxylase-deficient strains has been mapped to the cadA gene at 93.7 minutes, while the Mudlac fusions exhibiting a deficiency in the inducible arginine decarboxylase have been mapped to 93.4 minutes.</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2527331 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241709'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.U480cfd2cbc167&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:2662934

2008-04-21T20:46:11Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Auger, EA and Bennett, GN''' (1989) Regulation of lysine decarboxylase activity in Escherichia coli K-12.''Arch. Microbiol.'' '''151''':466-8

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The biodegradative lysine decarboxylase of E. coli has been reported to attain a higher specific activity when grown to saturation in the presence of excess lysine under conditions of low pH and absence of aeration. In order to examine possible sources of the pH and anaerobic regulation, a series of isogenic strains of E. coli K-12 were constructed. The effects of cadR-, fnr-, cya-, crp- and pgi- mutations on lysine decarboxylase expression were examined. Cultures were grown in a lysine supplemented rich medium at pH 5.5, pH 6.8, and pH 8.0 with and without aeration and the enzyme was assayed from log phase cultures. The results suggested that the pH and air responses were independent and that these known regulatory processes are not responsible for this regulation of the biodegradative lysine decarboxylase.</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2662934 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241708'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X480cfd11f3e42&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:3019489

2008-04-21T20:45:19Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Aufrère, R, Tempête, M and Bohin, JP''' (1986) [A case of overlapping reading frames in Escherichia coli]''C. R. Acad. Sci. III, Sci. Vie'' '''303''':49-54

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The two promoters and the first 65 nucleotides of a gene related to the rrnB operon are localized in the coding sequence of the btuB gene.</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3019489 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241707'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.I480cfcdde71a9&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:3515321

2008-04-21T20:44:02Z

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{{RightTOC}}

 '''Arikan, E, Kulkarni, MS, Thomas, DC and Sancar, A''' (1986) Sequences of the E. coli uvrB gene and protein.''Nucleic Acids Res.'' '''14''':2637-50

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The UvrB protein is one of the three subunits of the E. coli ABC excinuclease. We have reported the sequences of the other two subunits, the UvrA and UvrC proteins. In this paper the sequence of the UvrB protein is presented. The protein sequence was determined from the DNA sequence of the uvrB gene and was confirmed by sequencing the NH2-terminus of the UvrB protein and analyzing its overall amino acid composition. The coding region of uvrB is 2019 basepairs, specifying a protein of 672 amino acids and Mr of 76,118. The sequence of the UvrB protein shows a moderate level of homology to that of the UvrC protein and to the ATP binding site of the UvrA protein. During purification of UvrB protein a proteolytic product, UvrB, is produced in high quantities. We find that UvrB results from removal of about 40 amino acids from the COOH-terminus of the UvrB protein. The uvrB gene has complex regulatory features. On the 5' side, the coding region is preceded by 3 promoters, a DnaA box and an SOS box. On the 3' side the gene is followed by an REP (Repetitive Extragenic Palindrome) sequence which has been implicated in gene regulation by an unknown mechanism.</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3515321 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

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{{LitMaterials}}

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<protect>

{|class = 'sortable' {{Prettytable}} id='241706'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.P480cfc90df0a6&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

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==References==
{{RefHelp}}
<references/>

PMID:4576394

2008-04-21T20:42:45Z

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{{RightTOC}}

 '''Archibold, ER and Williams, LS''' (1973) Regulation of methionyl-transfer ribonucleic acid synthetase formation in Escherichia coli and Salmonella typhimurium.''J. Bacteriol.'' '''114''':1007-13

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>The control of methionyl-transfer ribonucleic acid (tRNA) synthetase (l-methionine: soluble RNA ligase [adenosine monophosphate]) was studied in methionyl-tRNA synthetase mutants of Escherichia coli and Salmonella typhimurium. The results of activity determinations with crude extracts indicate that this enzyme of the E. coli mutant strain possessed a reduced affinity for methionine-tRNA, whereas this enzyme of the S. typhimurium mutant exhibited a decreased affinity for l-methionine. The differential rate of methionyl-tRNA synthetase formation in these two mutants was several-fold greater than that of the respective parental strains. On the other hand, the level of in vivo aminoacylation of methionine-tRNA was only about one-third that of the parent strains. These results suggest that aminoacylation of methionine-tRNA is a necessary step in repression control of methionyl-tRNA synthetase of both E. coli and S. typhimurium strains.</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4576394 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241705'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J480cfc44d2dec&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:6154596

2008-04-21T20:38:31Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Apirion, D and Watson, N''' (1980) A second gene which affects the RNA processing enzyme ribonuclease P of Escherichia coli.''FEBS Lett.'' '''110''':161-3

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>No abstract in PubMed</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6154596 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241704'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.X480cfb464f4f8&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:4895052

2008-04-21T20:37:52Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Apirion, D and Schlessinger, D''' (1968) Coresistance to neomycin and kanamycin by mutations in an Escherichia coli locus that affects ribosomes.''J. Bacteriol.'' '''96''':768-76

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Mutant strains resistant to neomycin or to kanamycin sulfate were isolated from Escherichia coli K-12. Nine mutants were analyzed; all were resistant to both antibiotics (about 150 and 100 mug/ml, respectively), and were designated nek. In the mutant strains, the ribosomes are changed from those of the parental strain; for when they were used in assays for polypeptide formation directed by polyadenylic acid or polycytidylic acid, coding fidelity in presence of the drugs was increased and inhibition of synthesis by the drugs was lessened. Mating experiments and transduction tests showed that all of the nine nek mutants are either closely linked or allelic, and the nek locus is closely linked to two genes-str (streptomycin) and spc (spectinomycin)-known to affect the 30S ribosome. The two nek mutants tested were recessive to the sensitive, wild-type allele. When the nek mutants were compared to the parental strain, pleiotropic effects of the nek mutations were observed. Resistance to low levels of streptomycin and spectinomycin was increased, whereas resistance to chloramphenicol was decreased. Also, the mutants were less able to adapt to high concentrations of lincomycin, and could no longer show phenotypic suppression of an arginine requirement by neomycin or kanamycin. Such pleiotropic effects are suggested to be the rule for mutations in genes that participate in the biosynthesis of a cellular organelle.</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4895052 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241703'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.U480cfb1e9ab80&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:4870510

2008-04-21T20:36:45Z

Mattnat08: New PMID: Page!

{{RightTOC}}

 '''Apirion, D''' (1967) Three genes that affect Escherichia coli ribosomes.''J. Mol. Biol.'' '''30''':255-75

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>No abstract in PubMed</protect>
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4870510 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' {{Prettytable}} id='241702'
|- {{table heading style}}
!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.H480cfadc137f2&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

EcoliWiki:Tracking Plasmids

2008-04-04T07:54:08Z

Mattnat08: edited by Mattnat08 via TableEdit

This is a page to track plasmids entered into the wiki. It is for developmental purposes only.

<protect>

{|class = 'sortable' {{Prettytable}} id='220572'
|-
!Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed!!Date Info Added!!Date Reviewed
|-
|
LITMUS 28i
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.03.07
|
11.12.07
|
11.13.07
|-
|
LITMUS 38i
|
NEB
|
Miriam Garcia
|
08.31.2007
|
Miriam Garcia
|
08.31.07
|
10.31.07
|
10.31.07
|-
|
pGPS1.1
|
NEB
|
Charner Williams
|
08.31.07
|
Miriam Garcia
|
09.04.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pGPS2.1
|
NEB
|
Matthew Lakich
|
09.04.07
|
Miriam Garcia
|
09.06.07
|
12.04.07
|
12.17.07
|-
|
pGPS3
|
NEB
|
Grant Richardson
|
09.07.07
|
Miriam Garcia
|
09.10.07
|
Aaron Hopkins
01.22.08
|
01.23.08
|-
|
pGPS4
|
NEB
|
Amy Rabb
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
Joseph Kim
01.22.08
|
01.23.08
|-
|
pGPS5
|
NEB
|
Stephanie Michinock
|
09.03.07
|
Miriam Garcia
|
09.04.07
|
11.12.07
|
11.13.07
|-
|
pMAL-c4E
|
NEB
|
Amy Rabb
|
09.10.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.21.08
|
01.23.08
|-
|
pMAL-c4G
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
Katrina Trapp
01.31.08
|
11.13.07
|-
|
pMAL-c4X
|
NEB
|
Matthew Lakich
|
9.13.07
|
Miriam Garcia
|
09.14.07
|
12.17.07 Miriam Garcia
|
12.17.07
|-
|
pMAL-p4E
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.07.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4G
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-p4X
|
NEB
|
Charner Williams
|
09.07.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pMAL-pIII
|
NEB
|
Charner Williams
|
09.08.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 11.16.07
|
11.16.07
|-
|
pNEB193
|
NEB
|
CharnerWilliams
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pNEB206A
|
NEB
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
Miriam Garcia 09.19.07
|
09.19.07
|-
|
pTWIN1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTXB3
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.12.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB1
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pTYB11
|
NEB
|
Amy Rabb
|
9.12.07
|
Miriam Garcia
|
09.13.07
|
Aaron Hopkins
01.29.08
|
01.30.08
|-
|
pUC19
|
NEB
|

|

|
Miriam Garcia
|
09.26.07
|
Aaron Hopkins
|

|-
|
pNEBR-R1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1GLuc
|
NEB
|
Stephanie Michinock
|
09.08.07
|
Miriam Garcia
|
09.10.07
|
11.18.07
|
11.19.07
|-
|
pNEBR-X1Hygro
|
NEB
|
Grant Richardson
|
09.10.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pKLAC1-malE
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pCMV-GLuc
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pGLuc-Basic Vector
|
NEB
|
Grant Richardson
|
09.11.07
|
Miriam Garcia
|
09.12.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-Q
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP
|
Stratagene
|
Grant Richardson
|
09.12.07
|
Miriam Garcia
|
09.13.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-Q
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SBP-SET1
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET1-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET2-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.13.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SBP-SET3-Q
|
Stratagene
|
Stephanie Michinock
|
09.13.07
|
Miriam Garcia
|
09.14.07
|
12.02.07
|
12.03.07
|-
|
pBEc-SET1
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Joseph Kim
01.30.08
|
02.01.08
|-
|
pBEc-SET2
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEc-SET3
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1a
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.31.08
|-
|
pBEn-SBP-SET1b
|
Stratagene
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.23.07
|
Matthew Lakich
1.30.08
|
01.30.08
|-
|
pBEn-SBP-SET1c
|
Stratagene
|
Charner Williams
|
09.13.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.21.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET2c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBP-SET3c
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SBPa
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPb
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Matthew Lakich
1.25.08
|
01.25.08
|-
|
pBEn-SBPc
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.14.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pBEn-SET1a
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.24.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET1c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET2c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3a
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3b
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pBEn-SET3c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-c
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-kc
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n
|
Stratagene
|
Charner Williams
|
09.21.07
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia 11.19.07
|
11.19.07
|-
|
pCAL-n-EK
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pCAL-n-FLAG
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
01.31.08
|
01.31.08
|-
|
pET-11a
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11b
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp
02.05.08
|
02.07.08
|-
|
pET-11c
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Katrina Trapp 02.05.08
|
02.07.08
|-
|
pET-11d
|
Stratagene
|
Stephanie Michinock
|
09.18.07
|
Miriam Garcia
|
09.18.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3a
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.04.08
|
02.13.08
|-
|
pET-3b
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3c
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET-3d
|
Stratagene
|
Grant Richardson
|
09.14.07
|
Miriam Garcia
|
09.17.07
|
Aaron Hopkins
02.05.08
|
02.13.08
|-
|
pET100/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
09.10.07
|

|

|
11.01.07
|

|-
|
pET-14b
|
Novagen
|
Miriam Garcia
|
09.19.07
|

|

|
11.07.07
|

|-
|
pET-15b
|
Novagen
|
Matthew Lakich
|
9.20.07
|
Miriam Garcia
|
09.21.07
|
Aaron Hopkins 02.05.08
|
02.13.08
|-
|
pET-16b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-17b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-19b
|
Novagen
|
Matthew Lakich
|
9.21.07
|
Miriam Garcia
|
09.23.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-20b(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21(+)
|
Novagen
|
Matthew Lakich
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21a(+)
|
Novagen
|
Matthew Lakich
|
10.2.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21b(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21c(+)
|
Novagen
|
Grant Richardson
|
09.24.07
|
Miriam Garcia
|
09.25.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-21d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Joseph Kim
02.12.08
|
02.13.08
|-
|
pET-22b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23a(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-23b(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23c(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich
2.5.08
|
02.07.08
|-
|
pET-23d(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Matthew Lakich

2.5.08
|
02.07.08
|-
|
pET-24(+)
|
Novagen
|
Stephanie Michinock
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
02.13.08
|
02.14.08
|-
|
pET-24a(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24b(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24c(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-24d(+)
|
Novagen
|
Grant Richardson
|
09.25.07
|
Miriam Garcia
|
09.26.07
|
Katrina Trapp
2.19.08
|
02.20.08
|-
|
pET-25b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Katrina Trapp
02.26.07
|
02.27.07
|-
|
pET-26b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-27b(+)
|
Novagen
|
Amy Rabb
|
9.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.07.08
|-
|
pET-28b(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-28c(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29a(+)
|
Novagen
|
Grant Richardson
|
09.27.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-29b(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-29c(+)
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Miriam Garcia
01.30.08
|
01.30.08
|-
|
pET-30a(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-30c(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-31b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32a(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32b(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-32c(+) DNA
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-33b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-39b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-40b(+)
|
Novagen
|
Charner Williams
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia 11.26.07
|
11.26.07
|-
|
pET-41a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-41c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42a(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.03.08
|
02.08.08
|-
|
pET-42b(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
01.03.08
|
02.08.08
|-
|
pET-42c(+)
|
Novagen
|
Amy Rabb
|
10.1.07
|
Miriam Garcia
|
10.01.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-43.1c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44a(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-44c(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-45b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-47b(+)
|
Novagen
|
Amy Rabb
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-48b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-49b(+)
|
Novagen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Pei Yap
02.08.08
|
02.13.08
|-
|
pET-50b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Pei Yap
02.09.08
|
02.13.08
|-
|
pET-51b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-52b(+)
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9a
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9b
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9c
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pET-9d
|
Novagen
|
Grant Richardson
|
09.28.07
|
Miriam Garcia
|
09.28.07
|
Aaron Hopkins
02.20.08
|
02.20.08
|-
|
pETcoco-1
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pETcoco-2
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLacI
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysE
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pLysS
|
Novagen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET100/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.3.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET101/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D/LacZ
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.04.07
|
Joseph Kim
02.17.08
|
02.18.08
|-
|
pET102/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D/LacZ
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET151/D-TOPO
|
Invitrogen
|
Grant Richardson
|
10.02.07
|
Miriam Garcia
|
10.02.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160-GW/CAT
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET160/GW/D-TOPO
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161-GW/CAT
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Matthew Lakich
02.24.08
|
02.25.08
|-
|
pET161/GW/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D/LacZ
|
Invitrogen
|
Miriam Garcia
|
10.08.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
02.27.08
|
02.27.08
|-
|
pET200/D-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pET-DEST42
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST15
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST14
|
Invitrogen
|
Stephanie Michinock
|
10.05.07
|
Miriam Garcia
|
10.08.07
|
Katrina Trapp
2.27.08
|
02.29.08
|-
|
pDEST17
|
Invitrogen
|
Matthew Lakich
|
10.4.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pDEST24
|
Invitrogen
|
Matthew Lakich
|
10.04.07
|
Miriam Garcia
|
10.08.07
|
Pei Yap 2.19.08
|
02.20.08
|-
|
pRSET T7
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pRSET-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pCRT7-E
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
2.29.08
|-
|
pBAD102/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD102/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D/LacZ
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD202/D-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD/Thio-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
Pei Yap 2.19.08
|
02.29.08
|-
|
pBAD-TOPO
|
Invitrogen
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
Pei 2.19.08
|
02.29.08
|-
|
pBAD-TOPO/LacZ/V5-His
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD-DEST49
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His/LacZ
|
Invitrogen
|
Grant Richardson
|
10.04.07
|
Miriam Garcia
|
10.04.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
1.23.08
|-
|
pBAD/His B
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/His C
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.13.08
|
01.23.08
|-
|
pBAD/myc-His A
|
Invitrogen
|
Matthew Lakich
|
10.5.07
|
Miriam Garcia
|
10.08.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His B
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/myc-His C
|
Invitrogen
|
Matthew Lakich
|
10.11.07
|
Miriam Garcia
|
10.12.07
|
1.17.08
|
01.23.08
|-
|
pBAD/Myc-His/lacZ
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.17.08
|
01.23.08
|-
|
pBAD/gIII Calmodulin
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
AH 02.28.08
|
03.04.08
|-
|
pBAD/gIII A
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII B
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pBAD/gIII C
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.07
|
01.23.08
|-
|
pBAD/Thio-E
|
Invitrogen
|
Matthew Lakich
|
10.15.07
|
Miriam Garcia
|
10.15.07
|
1.13.08
|
01.23.08
|-
|
pThioHis A
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
AH 02.28.08
|
03.04.08
|-
|
pThioHis B
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pThioHis C
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis-TOPO/lacZ
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.04.08
|-
|
pTrcHis2-TOPO
|
Invitrogen
|
Stephanie Michinock
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis2-TOPO/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis/CAT
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
JK
02.26.08
|
03.17.08
|-
|
pTrcHis C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
Matthew Lakich 03.31.08
|

|-
|
pTrcHis2/LacZ
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
Matthew Lakich 03.31.08
|

|-
|
pTrcHis2 A
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
Matthew Lakich 03.31.08
|

|-
|
pTrcHis2 B
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
Matthew Lakich 03.31.08
|

|-
|
pTrcHis2 C
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
Matthew Lakich 03.31.08
|

|-
|
pCX
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
Matthew Lakich 03.31.08
|

|-
|
pCX-TOPO
|
Invitrogen
|
Grant Richardson
|
10.09.07
|
Miriam Garcia
|
10.10.07
|
KT
2.27.08
|
2.29.08
|-
|
pET-30 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-32 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-41 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-43.1 Ek/LIC
|
Novagen
|
Charner Williams
|
Miriam Garcia 11.26.07
|
Miriam Garcia
|
11.26.07
|
11.26.07
|
11.26.07
|-
|
pET-44 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-46 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pET-51 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.12.07
|
Miriam Garcia
|
11.13.07
|
11.12.07
|
11.13.07
|-
|
pCDF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pRSF-2 Ek/LIC
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBAC-2cp
|
Novagen
|
Stephanie Michinock
|
11.17.07
|
Miriam Garcia
|
11.19.07
|
11.17.07
|
11.19.07
|-
|
pBACgus-2cp
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-30 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-32 Xa/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pET-52 3c/LIC
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pETDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pACYCDuet-1
|
Novagen
|
Amy Rabb
|
12.13.07
|
Miriam Garcia
|
12.17.07
|
12.13.07
|
12.17.07
|-
|
pCOLADuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.28.07
|
11.27.07
|
11.28.07
|-
|
pCDFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pRSFDuet-1
|
Novagen
|
Stephanie Michinock
|
11.27.07
|
Miriam Garcia
|
11.29.07
|
11.27.07
|
11.29.07
|-
|
pETBlue-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pETBlue-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-1
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-2
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pBiEx-3
|
Novagen
|
Amy Rabb
|
12/12/07
|
Miriam Garcia
|
01.14.07
|
12/12/07
|
01.14.07
|-
|
pF1A T7
|
Promega
|
Miriam Garcia
|
09.27.07
|
Miriam Garcia
|
11.07.07
|
KT
2.27.08
|
2.29.08
|-
|
pF1K T7
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2A (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pFN2K (GST)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.08
|
02.29.08
|-
|
pF3A WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
KT
2.27.28
|
02.29.08
|-
|
pF3K WG (BYDV)
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4A CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.19.08
|-
|
pF4K CMV
|
Promega
|
Grant Richardson
|
10.11.07
|
Miriam Garcia
|
10.11.07
|
PY 03.04.08
|
03.20.08
|-
|
pF5A CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pF5K CMV-neo
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFN6A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.04.08
|
03.20.08
|-
|
pFN6K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7A (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC7K (HQ)
|
Promega
|
Stephanie Michinock
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
PY 03.05.08
|
03.20.08
|-
|
pFC8A (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFC8K (HaloTag)
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF9A CMV hRluc-neo
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN10A (ACT)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pFN11A (BIND)
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH 03.05.08
|
03.17.08
|-
|
pF12A RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
AH
|

|-
|
pF12K RM
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
|

|-
|
PinPoint Xa-1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
JK
03.02.08
|
03.17.08
|-
|
pALTER-EX1
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pALTER-EX2
|
Promega
|
Amy Rabb
|
10.21.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pET-5a
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5b
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pET-5c
|
Promega
|
Amy Rabb
|
10.14.07
|
Miriam Garcia
|
10.15.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-1
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
pGEMEX-2
|
Promega
|
Amy Rabb
|
10.18.07
|
Miriam Garcia
|
10.22.07
|
12.13.07
|
1.14.07
|-
|
PinPoint Control
|
Promega
|
Matthew Lakich
|
10.28.07
|
Miriam Garcia
|
11.05.07
|
1.10.08
|
01.23.08
|-
|
PinPoint Xa Control
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-2
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
PinPoint Xa-3
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Z
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(+)
|
Promega
|
Matthew Lakich
|
10.31.07
|
Miriam Garcia
|
11.05.07
|
1.11.08
|
01.23.08
|-
|
pGEM-3Zf(-)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-4Z
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(+)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-5Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(+)
|
Promega
|
Grant Richadson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-7Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-9Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
JK
03.02.08
|
03.17.08
|-
|
pGEM-11Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
Matthew Lakich 04.03.08
|

|-
|
pGEM-11Zf(-)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
Matthew Lakich 04.03.08
|

|-
|
pGEM-13Zf(+)
|
Promega
|
Grant Richardson
|
10.17.07
|
Miriam Garcia
|
10.18.07
|
Matthew Lakich 04.03.08
|

|-
|
pGL2-Basic
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
Matthew Lakich 04.03.08
|

|-
|
pGL2-Control
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
Matthew Lakich 04.03.08
|

|-
|
pGL2-Enhancer
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
Matthew Lakich 04.03.08
|

|-
|
pGL2-Promoter
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pG5luc
|
Promega
|
Grant Richardson
|
10.18.07
|
Miriam Garcia
|
10.19.07
|
KT
3.26.08
|

|-
|
pSP64 Poly(A)
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
3.26.08
|

|-
|
pSP72
|
Promega
|
Grant Richardson
|
10.23.07
|
Miriam Garcia
|
10.24.07
|
KT
3.26.08
|

|-
|
pSP73
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
KT
3.26.08
|

|-
|
pGEM-1
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
KT
3.26.08
|

|-
|
pGEM-2
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-3
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-4 Vector
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pGEM-15Zf(-)
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
11.07.07
|
12.13.07
|
01.16.07
|-
|
pSP64
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP65
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pSP70
|
Promega
|
Amy Rabb
|
10.26.07
|
Miriam Garcia
|
10.29.07
|
12.13.07
|
01.16.07
|-
|
pBEn-SBP-SET3b
|
Stratagene
|
Charner Williams
|
09.14.07
|
Miriam Garcia
|
09.27.07
|

|

|-
|
pRS414
|
Simons Lab
|
Miriam Garcia
|
10.25.07
|

|

|
10.26.07
|
11.14.07
|-
|
pRS415
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.13.07
|
11.11.07
|
11.14.07
|-
|
pRS1274
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS591
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS552
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS551
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS577
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS550
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS308
|
Simons Lab
|
Amy Rabb
|
11.12.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-
|
pRS475
|
Simons Lab
|
Amy Rabb
|
11.11.07
|
Miriam Garcia
|
11.14.07
|
11.14.07
|
11.14.07
|-class='sortbottom'
|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || ||
|}
</protect>

pGL2-Enhancer

2008-04-04T07:51:53Z

Mattnat08: /* References */

==At a Glance==
[[Image:pGL2-Enhancer.gif|thumb|right|350px|pGL2-Enhancer, image provided by [http://www.promega.com/figures/popup.asp?fn=0314va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-e.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region

Luciferase gene (luc)

GLprimer2 binding site

SV40 late poly(A) signal

SV40 Enhancer

RVprimer4 binding site

ColE1-derived plasmid replication origin

lactamase (Ampr) gene

GLprimer1 binding site
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650341 X65325]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Z47176b3de3da6&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pGL2-Enhancer Vector contains an SV40 enhancer, derived from the SV40 early promoter, located downstream of the luciferase gene after the poly(A) signal. This aids in the verification of functional promoter elements because the presence of the enhancer in many cases will result in transcription of luc at higher levels.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DEnhancer+Vector&description%5Ftext=pGL2%2DEnhancer+Vector Promega]

==References==

<ref name='PMID:2889129'/>
<ref name='PMID:3323819'/>
<references/>

==Sequence==
<pre>
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC
51 TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA
101 AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA
151 CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT
201 TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA
251 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT
301 CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT
351 GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT
401 ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA
451 GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA
501 ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC
551 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT
601 TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT
651 TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG
701 CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT
751 ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC
801 ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG
851 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA
901 TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT
951 TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT
1001 TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA
1051 GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT
1101 CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC
1151 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT
1201 CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT
1251 CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA
1301 ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC
1351 TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT
1401 TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT
1451 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT
1501 GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC
1551 GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG
1601 CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT
1651 CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC
1701 CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG
1751 ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT
1801 AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC
1851 TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG
1901 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA
1951 GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT
2001 TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA
2051 CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT
2101 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA
2151 ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT
2201 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA
2251 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT
2301 ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA
2351 ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA
2401 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT
2451 AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA
2501 GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA
2551 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
2601 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
2651 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
2701 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCTGAACCA
2751 TGGAGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG
2801 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA
2851 TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC
2901 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA
2951 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCAGATCC TCTAGGGATC
3001 CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG
3051 GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA
3101 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC
3151 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
3201 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
3251 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
3301 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
3351 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
3401 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
3451 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
3501 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
3551 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
3601 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
3651 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
3701 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
3751 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
3801 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
3851 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
3901 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
3951 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
4001 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
4051 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
4101 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
4151 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
4201 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA
4251 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
4301 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
4351 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
4401 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
4451 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
4501 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC
4551 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
4601 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
4651 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
4701 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
4751 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
4801 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA
4851 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
4901 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
4951 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
5001 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
5051 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT
5101 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG
5151 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
5201 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT
5251 ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
5301 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG
5351 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT
5401 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT
5451 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
5501 ATATTAACGC TTACAATTTG CCATTCGCCA TTCAGGCTGC GCAACTGTTG
5551 GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CCCAAGCTAC
5601 CATGATAAGT AAGTAATATT AAGGTACGTG GAGGTTTTAC TTGCTTTAAA
5651 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
5701 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
5751 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
5801 TGGTTTGTCC AAACTCATCA ATGTATCTTA TGGTACTGTA ACTGAGCTAA
5851 CATAA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Enhancer

2008-04-04T07:51:08Z

Mattnat08: edited by Mattnat08 via TableEdit

==At a Glance==
[[Image:pGL2-Enhancer.gif|thumb|right|350px|pGL2-Enhancer, image provided by [http://www.promega.com/figures/popup.asp?fn=0314va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-e.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region

Luciferase gene (luc)

GLprimer2 binding site

SV40 late poly(A) signal

SV40 Enhancer

RVprimer4 binding site

ColE1-derived plasmid replication origin

lactamase (Ampr) gene

GLprimer1 binding site
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650341 X65325]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Z47176b3de3da6&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pGL2-Enhancer Vector contains an SV40 enhancer, derived from the SV40 early promoter, located downstream of the luciferase gene after the poly(A) signal. This aids in the verification of functional promoter elements because the presence of the enhancer in many cases will result in transcription of luc at higher levels.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DEnhancer+Vector&description%5Ftext=pGL2%2DEnhancer+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC
51 TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA
101 AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA
151 CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT
201 TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA
251 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT
301 CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT
351 GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT
401 ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA
451 GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA
501 ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC
551 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT
601 TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT
651 TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG
701 CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT
751 ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC
801 ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG
851 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA
901 TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT
951 TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT
1001 TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA
1051 GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT
1101 CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC
1151 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT
1201 CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT
1251 CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA
1301 ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC
1351 TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT
1401 TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT
1451 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT
1501 GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC
1551 GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG
1601 CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT
1651 CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC
1701 CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG
1751 ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT
1801 AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC
1851 TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG
1901 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA
1951 GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT
2001 TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA
2051 CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT
2101 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA
2151 ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT
2201 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA
2251 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT
2301 ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA
2351 ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA
2401 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT
2451 AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA
2501 GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA
2551 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
2601 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
2651 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
2701 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCTGAACCA
2751 TGGAGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG
2801 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA
2851 TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC
2901 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA
2951 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCAGATCC TCTAGGGATC
3001 CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG
3051 GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA
3101 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC
3151 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
3201 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
3251 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
3301 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
3351 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
3401 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
3451 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
3501 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
3551 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
3601 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
3651 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
3701 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
3751 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
3801 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
3851 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
3901 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
3951 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
4001 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
4051 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
4101 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
4151 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
4201 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA
4251 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
4301 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
4351 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
4401 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
4451 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
4501 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC
4551 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
4601 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
4651 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
4701 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
4751 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
4801 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA
4851 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
4901 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
4951 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
5001 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
5051 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT
5101 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG
5151 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
5201 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT
5251 ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
5301 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG
5351 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT
5401 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT
5451 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
5501 ATATTAACGC TTACAATTTG CCATTCGCCA TTCAGGCTGC GCAACTGTTG
5551 GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CCCAAGCTAC
5601 CATGATAAGT AAGTAATATT AAGGTACGTG GAGGTTTTAC TTGCTTTAAA
5651 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
5701 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
5751 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
5801 TGGTTTGTCC AAACTCATCA ATGTATCTTA TGGTACTGTA ACTGAGCTAA
5851 CATAA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Enhancer

2008-04-04T07:49:32Z

Mattnat08: /* Notes */

==At a Glance==
[[Image:pGL2-Enhancer.gif|thumb|right|350px|pGL2-Enhancer, image provided by [http://www.promega.com/figures/popup.asp?fn=0314va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-e.txt Promega]
|-
!{{table heading style}} |Features
|
Contains an SV40 enhancer derived from the SV40 early promoter, located downstream of the luc gene after the poly(A) signal
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650341 X65325]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Z47176b3de3da6&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pGL2-Enhancer Vector contains an SV40 enhancer, derived from the SV40 early promoter, located downstream of the luciferase gene after the poly(A) signal. This aids in the verification of functional promoter elements because the presence of the enhancer in many cases will result in transcription of luc at higher levels.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DEnhancer+Vector&description%5Ftext=pGL2%2DEnhancer+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC
51 TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA
101 AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA
151 CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT
201 TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA
251 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT
301 CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT
351 GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT
401 ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA
451 GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA
501 ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC
551 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT
601 TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT
651 TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG
701 CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT
751 ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC
801 ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG
851 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA
901 TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT
951 TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT
1001 TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA
1051 GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT
1101 CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC
1151 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT
1201 CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT
1251 CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA
1301 ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC
1351 TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT
1401 TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT
1451 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT
1501 GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC
1551 GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG
1601 CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT
1651 CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC
1701 CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG
1751 ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT
1801 AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC
1851 TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG
1901 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA
1951 GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT
2001 TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA
2051 CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT
2101 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA
2151 ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT
2201 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA
2251 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT
2301 ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA
2351 ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA
2401 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT
2451 AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA
2501 GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA
2551 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
2601 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
2651 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
2701 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCTGAACCA
2751 TGGAGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG
2801 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA
2851 TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC
2901 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA
2951 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCAGATCC TCTAGGGATC
3001 CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG
3051 GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA
3101 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC
3151 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
3201 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG
3251 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
3301 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA
3351 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
3401 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
3451 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
3501 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
3551 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
3601 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
3651 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
3701 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
3751 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
3801 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
3851 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
3901 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
3951 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
4001 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
4051 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
4101 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
4151 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
4201 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA
4251 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
4301 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
4351 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
4401 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
4451 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
4501 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC
4551 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
4601 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
4651 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
4701 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
4751 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
4801 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA
4851 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
4901 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
4951 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
5001 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
5051 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT
5101 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG
5151 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC
5201 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT
5251 ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
5301 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG
5351 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT
5401 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT
5451 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA
5501 ATATTAACGC TTACAATTTG CCATTCGCCA TTCAGGCTGC GCAACTGTTG
5551 GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CCCAAGCTAC
5601 CATGATAAGT AAGTAATATT AAGGTACGTG GAGGTTTTAC TTGCTTTAAA
5651 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
5701 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
5751 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
5801 TGGTTTGTCC AAACTCATCA ATGTATCTTA TGGTACTGTA ACTGAGCTAA
5851 CATAA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Control

2008-04-04T07:48:09Z

Mattnat08: edited by Mattnat08 via TableEdit

==At a Glance==
[[Image:#pGL2-Control.gif|thumb|right|350px|pGL2-Control, image provided by [http://www.promega.com/figures/popup.asp?fn=0317va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-c.txt Promega]
|-
!{{table heading style}} |Features
|
SV40 Promoter

Luciferase gene (luc)

GLprimer2 binding site

SV40 late poly(A) signal

SV40 Enhancer

RVprimer4 binding site

ColE1-derived plasmid replication origin

lactamase (Ampr) gene

GLprimer1 binding site

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B47176b1fac2c9&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pGL2-Control Vector contains the SV40 promoter and enhancer sequences, resulting in strong luc expression in many types of mammalian cells. This plasmid is useful in monitoring transfection efficiency in general and is a convenient internal standard for promoter and enhancer activities expressed by pGL2 recombinants.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DControl+Vector&description%5Ftext=pGL2%2DControl+Vector Promega]

==References==

<ref name='PMID:3323819'/>
<ref name='PMID:2889129'/>
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCTGAAC CATGGAGCGG
2951 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG
3001 GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG
3051 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG
3101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC
3151 ACCCTAACTG ACACACATTC CACAGCAGAT CCTCTAGGGA TCCGTCGACC
3201 GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG
3251 GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC
3301 GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
3351 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
3401 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
3451 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
3501 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
3551 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
3601 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
3651 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
3701 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
3751 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
3801 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
3851 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
3901 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
3951 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
4001 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
4051 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
4101 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
4151 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
4201 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
4251 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
4301 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
4351 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
4401 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
4451 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
4501 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
4551 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
4601 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
4651 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
4701 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
4751 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
4801 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
4851 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
4901 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
4951 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
5001 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
5051 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
5101 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
5151 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
5201 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
5251 ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA
5301 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC
5351 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
5401 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
5451 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
5501 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
5551 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT
5601 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
5651 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC
5701 GCTTACAATT TGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC
5751 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCCCAAGCT ACCATGATAA
5801 GTAAGTAATA TTAAGGTACG TGGAGGTTTT ACTTGCTTTA AAAAACCTCC
5851 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
5901 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
5951 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
6001 CCAAACTCAT CAATGTATCT TATGGTACTG TAACTGAGCT AACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Control

2008-04-04T07:44:57Z

Mattnat08: /* Notes */

==At a Glance==
[[Image:#pGL2-Control.gif|thumb|right|350px|pGL2-Control, image provided by [http://www.promega.com/figures/popup.asp?fn=0317va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-c.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region

Luciferase gene (luc)

GLprimer2 binding site

SV40 late poly(A) signal

SV40 Enhancer

RVprimer4 binding site

ColE1-derived plasmid replication origin

β-lactamase (Ampr) gene

GLprimer1 binding site

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B47176b1fac2c9&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pGL2-Control Vector contains the SV40 promoter and enhancer sequences, resulting in strong luc expression in many types of mammalian cells. This plasmid is useful in monitoring transfection efficiency in general and is a convenient internal standard for promoter and enhancer activities expressed by pGL2 recombinants.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DControl+Vector&description%5Ftext=pGL2%2DControl+Vector Promega]

==References==

<ref name='PMID:3323819'/>
<ref name='PMID:2889129'/>
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCTGAAC CATGGAGCGG
2951 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG
3001 GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG
3051 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG
3101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC
3151 ACCCTAACTG ACACACATTC CACAGCAGAT CCTCTAGGGA TCCGTCGACC
3201 GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG
3251 GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC
3301 GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
3351 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
3401 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
3451 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
3501 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
3551 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
3601 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
3651 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
3701 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
3751 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
3801 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
3851 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
3901 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
3951 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
4001 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
4051 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
4101 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
4151 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
4201 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
4251 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
4301 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
4351 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
4401 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
4451 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
4501 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
4551 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
4601 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
4651 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
4701 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
4751 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
4801 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
4851 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
4901 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
4951 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
5001 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
5051 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
5101 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
5151 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
5201 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
5251 ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA
5301 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC
5351 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
5401 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
5451 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
5501 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
5551 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT
5601 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
5651 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC
5701 GCTTACAATT TGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC
5751 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCCCAAGCT ACCATGATAA
5801 GTAAGTAATA TTAAGGTACG TGGAGGTTTT ACTTGCTTTA AAAAACCTCC
5851 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
5901 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
5951 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
6001 CCAAACTCAT CAATGTATCT TATGGTACTG TAACTGAGCT AACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Control

2008-04-04T06:42:17Z

Mattnat08: /* References */

==At a Glance==
[[Image:#pGL2-Control.gif|thumb|right|350px|pGL2-Control, image provided by [http://www.promega.com/figures/popup.asp?fn=0317va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-c.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region

Luciferase gene (luc)

GLprimer2 binding site

SV40 late poly(A) signal

SV40 Enhancer

RVprimer4 binding site

ColE1-derived plasmid replication origin

β-lactamase (Ampr) gene

GLprimer1 binding site

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B47176b1fac2c9&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pGL2-Enhancer Vector contains an SV40 enhancer, derived from the SV40 early promoter, located downstream of the luciferase gene after the poly(A)signal. This aids in the verification of functional promoter elements because the presence of the enhancer in many cases will result in transcription of luc at higher levels.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DControl+Vector&description%5Ftext=pGL2%2DControl+Vector Promega]

==References==

<ref name='PMID:3323819'/>
<ref name='PMID:2889129'/>
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCTGAAC CATGGAGCGG
2951 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG
3001 GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG
3051 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG
3101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC
3151 ACCCTAACTG ACACACATTC CACAGCAGAT CCTCTAGGGA TCCGTCGACC
3201 GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG
3251 GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC
3301 GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
3351 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
3401 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
3451 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
3501 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
3551 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
3601 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
3651 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
3701 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
3751 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
3801 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
3851 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
3901 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
3951 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
4001 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
4051 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
4101 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
4151 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
4201 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
4251 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
4301 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
4351 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
4401 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
4451 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
4501 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
4551 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
4601 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
4651 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
4701 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
4751 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
4801 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
4851 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
4901 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
4951 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
5001 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
5051 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
5101 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
5151 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
5201 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
5251 ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA
5301 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC
5351 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
5401 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
5451 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
5501 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
5551 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT
5601 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
5651 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC
5701 GCTTACAATT TGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC
5751 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCCCAAGCT ACCATGATAA
5801 GTAAGTAATA TTAAGGTACG TGGAGGTTTT ACTTGCTTTA AAAAACCTCC
5851 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
5901 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
5951 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
6001 CCAAACTCAT CAATGTATCT TATGGTACTG TAACTGAGCT AACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Control

2008-04-04T06:41:49Z

Mattnat08: edited by Mattnat08 via TableEdit

==At a Glance==
[[Image:#pGL2-Control.gif|thumb|right|350px|pGL2-Control, image provided by [http://www.promega.com/figures/popup.asp?fn=0317va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-c.txt Promega]
|-
!{{table heading style}} |Features
|
Multiple cloning region

Luciferase gene (luc)

GLprimer2 binding site

SV40 late poly(A) signal

SV40 Enhancer

RVprimer4 binding site

ColE1-derived plasmid replication origin

β-lactamase (Ampr) gene

GLprimer1 binding site

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B47176b1fac2c9&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pGL2-Enhancer Vector contains an SV40 enhancer, derived from the SV40 early promoter, located downstream of the luciferase gene after the poly(A)signal. This aids in the verification of functional promoter elements because the presence of the enhancer in many cases will result in transcription of luc at higher levels.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DControl+Vector&description%5Ftext=pGL2%2DControl+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCTGAAC CATGGAGCGG
2951 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG
3001 GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG
3051 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG
3101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC
3151 ACCCTAACTG ACACACATTC CACAGCAGAT CCTCTAGGGA TCCGTCGACC
3201 GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG
3251 GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC
3301 GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
3351 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
3401 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
3451 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
3501 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
3551 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
3601 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
3651 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
3701 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
3751 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
3801 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
3851 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
3901 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
3951 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
4001 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
4051 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
4101 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
4151 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
4201 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
4251 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
4301 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
4351 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
4401 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
4451 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
4501 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
4551 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
4601 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
4651 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
4701 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
4751 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
4801 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
4851 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
4901 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
4951 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
5001 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
5051 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
5101 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
5151 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
5201 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
5251 ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA
5301 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC
5351 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
5401 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
5451 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
5501 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
5551 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT
5601 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
5651 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC
5701 GCTTACAATT TGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC
5751 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCCCAAGCT ACCATGATAA
5801 GTAAGTAATA TTAAGGTACG TGGAGGTTTT ACTTGCTTTA AAAAACCTCC
5851 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
5901 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
5951 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
6001 CCAAACTCAT CAATGTATCT TATGGTACTG TAACTGAGCT AACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Control

2008-04-04T06:38:43Z

Mattnat08: /* Notes */

==At a Glance==
[[Image:#pGL2-Control.gif|thumb|right|350px|pGL2-Control, image provided by [http://www.promega.com/figures/popup.asp?fn=0317va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-c.txt Promega]
|-
!{{table heading style}} |Features
|
Contains SV40 promoter and enhancer sequences resulting in strong luc expression in many mammalian cells.

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650340 X65324]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B47176b1fac2c9&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==
The pGL2-Enhancer Vector contains an SV40 enhancer, derived from the SV40 early promoter, located downstream of the luciferase gene after the poly(A)signal. This aids in the verification of functional promoter elements because the presence of the enhancer in many cases will result in transcription of luc at higher levels.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DControl+Vector&description%5Ftext=pGL2%2DControl+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901 CCAAACTCAT CAATGTATCT TATCATGTCT GGATCTGAAC CATGGAGCGG
2951 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG
3001 GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG
3051 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG
3101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC
3151 ACCCTAACTG ACACACATTC CACAGCAGAT CCTCTAGGGA TCCGTCGACC
3201 GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG
3251 GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC
3301 GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
3351 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
3401 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
3451 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
3501 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
3551 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
3601 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
3651 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
3701 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
3751 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
3801 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
3851 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
3901 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
3951 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
4001 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
4051 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
4101 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
4151 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
4201 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
4251 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
4301 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
4351 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
4401 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
4451 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
4501 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
4551 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
4601 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
4651 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
4701 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
4751 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
4801 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
4851 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
4901 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
4951 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
5001 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
5051 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
5101 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
5151 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
5201 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
5251 ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA
5301 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC
5351 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
5401 TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
5451 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
5501 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
5551 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT
5601 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
5651 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC
5701 GCTTACAATT TGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC
5751 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCCCAAGCT ACCATGATAA
5801 GTAAGTAATA TTAAGGTACG TGGAGGTTTT ACTTGCTTTA AAAAACCTCC
5851 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
5901 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
5951 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
6001 CCAAACTCAT CAATGTATCT TATGGTACTG TAACTGAGCT AACATAA

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Basic

2008-04-04T06:35:30Z

Mattnat08: /* References */

==At a Glance==
[[Image:#pGL2-Basic.gif|thumb|right|350px|pGL2-Basic, image provided by [http://www.promega.com/figures/popup.asp?fn=0313va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-b.txt Promega]
|-
!{{table heading style}} |Features
|
SV40 Promoter

SV40 Enhancer

Multiple cloning region

GLprimer2 binding site

Luciferase gene (''luc'')

SV40 late poly(A) signal

RVprimer4 binding site

beta-lactamase (Amp res) gene

ColE1-derived plasmid replication origin

GLprimer1 binding site
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650339 X65323]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.D47176b06a95f8&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==

The pGL2-Basic Vector lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences. Expression of luciferase activity in cells transfected with this plasmid depends on insertion and proper orientation of a functional promoter upstream from luc. Potential enhancer elements can also be inserted upstream of the promoter or in the BamHI or SalI sites downstream of the luciferase gene.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DBasic+Vector&description%5Ftext=pGL2%2DBasic+Vector Promega]

==References==

<ref name='PMID:3323819'/>
<ref name='PMID:2889129'/>
<references/>

==Sequence==

<pre>
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC

51 TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA

101 AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA

151 CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT

201 TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA

251 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT

301 CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT

351 GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT

401 ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA

451 GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA

501 ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC

551 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT

601 TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT

651 TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG

701 CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT

751 ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC

801 ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG

851 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA

901 TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT

951 TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT

1001 TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA

1051 GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT

1101 CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC

1151 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT

1201 CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT

1251 CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA

1301 ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC

1351 TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT

1401 TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT

1451 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT

1501 GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC

1551 GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG

1601 CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT

1651 CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC

1701 CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG

1751 ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT

1801 AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC

1851 TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG

1901 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA

1951 GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT

2001 TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA

2051 CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT

2101 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA

2151 ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT

2201 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA

2251 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT

2301 ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA

2351 ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA

2401 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT

2451 AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA

2501 GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA

2551 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG

2601 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT

2651 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG

2701 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCCGTCGAC

2751 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG

2801 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT

2851 CGTAGGACAG GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG

2901 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC

2951 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT

3001 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG

3051 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG

3101 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

3151 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

3201 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA

3251 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC

3301 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC

3351 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT

3401 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

3451 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA

3501 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT

3551 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT

3601 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT

3651 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG

3701 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

3751 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA

3801 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC

3851 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA

3901 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA

3951 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC

4001 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA

4051 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT

4101 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG

4151 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC

4201 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT

4251 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT

4301 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT

4351 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG

4401 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC

4451 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC

4501 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC

4551 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC

4601 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG

4651 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA

4701 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT

4751 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC

4801 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT

4851 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT

4901 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG

4951 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC

5001 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC

5051 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA

5101 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC

5151 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA

5201 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA

5251 CGCTTACAAT TTGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG

5301 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCCCAAGC TACCATGATA

5351 AGTAAGTAAT ATTAAGGTAC GTGGAGGTTT TACTTGCTTT AAAAAACCTC

5401 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT

5451 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA

5501 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG

5551 TCCAAACTCA TCAATGTATC TTATGGTACT GTAACTGAGC TAACATAA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Basic

2008-04-04T06:27:59Z

Mattnat08: edited by Mattnat08 via TableEdit

==At a Glance==
[[Image:#pGL2-Basic.gif|thumb|right|350px|pGL2-Basic, image provided by [http://www.promega.com/figures/popup.asp?fn=0313va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-b.txt Promega]
|-
!{{table heading style}} |Features
|
SV40 Promoter

SV40 Enhancer

Multiple cloning region

GLprimer2 binding site

Luciferase gene (''luc'')

SV40 late poly(A) signal

RVprimer4 binding site

beta-lactamase (Amp res) gene

ColE1-derived plasmid replication origin

GLprimer1 binding site
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650339 X65323]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.D47176b06a95f8&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==

The pGL2-Basic Vector lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences. Expression of luciferase activity in cells transfected with this plasmid depends on insertion and proper orientation of a functional promoter upstream from luc. Potential enhancer elements can also be inserted upstream of the promoter or in the BamHI or SalI sites downstream of the luciferase gene.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DBasic+Vector&description%5Ftext=pGL2%2DBasic+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==

<pre>
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC

51 TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA

101 AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA

151 CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT

201 TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA

251 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT

301 CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT

351 GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT

401 ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA

451 GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA

501 ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC

551 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT

601 TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT

651 TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG

701 CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT

751 ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC

801 ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG

851 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA

901 TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT

951 TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT

1001 TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA

1051 GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT

1101 CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC

1151 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT

1201 CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT

1251 CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA

1301 ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC

1351 TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT

1401 TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT

1451 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT

1501 GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC

1551 GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG

1601 CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT

1651 CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC

1701 CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG

1751 ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT

1801 AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC

1851 TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG

1901 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA

1951 GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT

2001 TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA

2051 CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT

2101 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA

2151 ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT

2201 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA

2251 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT

2301 ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA

2351 ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA

2401 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT

2451 AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA

2501 GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA

2551 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG

2601 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT

2651 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG

2701 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCCGTCGAC

2751 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG

2801 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT

2851 CGTAGGACAG GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG

2901 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC

2951 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT

3001 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG

3051 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG

3101 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

3151 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

3201 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA

3251 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC

3301 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC

3351 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT

3401 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

3451 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA

3501 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT

3551 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT

3601 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT

3651 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG

3701 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

3751 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA

3801 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC

3851 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA

3901 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA

3951 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC

4001 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA

4051 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT

4101 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG

4151 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC

4201 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT

4251 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT

4301 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT

4351 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG

4401 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC

4451 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC

4501 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC

4551 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC

4601 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG

4651 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA

4701 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT

4751 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC

4801 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT

4851 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT

4901 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG

4951 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC

5001 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC

5051 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA

5101 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC

5151 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA

5201 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA

5251 CGCTTACAAT TTGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG

5301 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCCCAAGC TACCATGATA

5351 AGTAAGTAAT ATTAAGGTAC GTGGAGGTTT TACTTGCTTT AAAAAACCTC

5401 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT

5451 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA

5501 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG

5551 TCCAAACTCA TCAATGTATC TTATGGTACT GTAACTGAGC TAACATAA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Basic

2008-04-04T06:25:11Z

Mattnat08: /* Notes */

==At a Glance==
[[Image:#pGL2-Basic.gif|thumb|right|350px|pGL2-Basic, image provided by [http://www.promega.com/figures/popup.asp?fn=0313va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-b.txt Promega]
|-
!{{table heading style}} |Features
|
Lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences.

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650339 X65323]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.D47176b06a95f8&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==

The pGL2-Basic Vector lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences. Expression of luciferase activity in cells transfected with this plasmid depends on insertion and proper orientation of a functional promoter upstream from luc. Potential enhancer elements can also be inserted upstream of the promoter or in the BamHI or SalI sites downstream of the luciferase gene.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DBasic+Vector&description%5Ftext=pGL2%2DBasic+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==

<pre>
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC

51 TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA

101 AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA

151 CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT

201 TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA

251 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT

301 CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT

351 GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT

401 ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA

451 GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA

501 ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC

551 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT

601 TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT

651 TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG

701 CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT

751 ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC

801 ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG

851 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA

901 TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT

951 TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT

1001 TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA

1051 GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT

1101 CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC

1151 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT

1201 CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT

1251 CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA

1301 ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC

1351 TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT

1401 TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT

1451 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT

1501 GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC

1551 GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG

1601 CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT

1651 CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC

1701 CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG

1751 ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT

1801 AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC

1851 TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG

1901 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA

1951 GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT

2001 TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA

2051 CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT

2101 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA

2151 ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT

2201 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA

2251 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT

2301 ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA

2351 ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA

2401 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT

2451 AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA

2501 GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA

2551 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG

2601 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT

2651 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG

2701 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCCGTCGAC

2751 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG

2801 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT

2851 CGTAGGACAG GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG

2901 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC

2951 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT

3001 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG

3051 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG

3101 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

3151 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

3201 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA

3251 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC

3301 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC

3351 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT

3401 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

3451 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA

3501 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT

3551 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT

3601 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT

3651 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG

3701 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

3751 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA

3801 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC

3851 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA

3901 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA

3951 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC

4001 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA

4051 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT

4101 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG

4151 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC

4201 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT

4251 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT

4301 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT

4351 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG

4401 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC

4451 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC

4501 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC

4551 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC

4601 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG

4651 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA

4701 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT

4751 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC

4801 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT

4851 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT

4901 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG

4951 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC

5001 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC

5051 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA

5101 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC

5151 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA

5201 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA

5251 CGCTTACAAT TTGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG

5301 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCCCAAGC TACCATGATA

5351 AGTAAGTAAT ATTAAGGTAC GTGGAGGTTT TACTTGCTTT AAAAAACCTC

5401 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT

5451 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA

5501 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG

5551 TCCAAACTCA TCAATGTATC TTATGGTACT GTAACTGAGC TAACATAA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGL2-Basic

2008-04-04T06:22:49Z

Mattnat08: /* Notes */

==At a Glance==
[[Image:#pGL2-Basic.gif|thumb|right|350px|pGL2-Basic, image provided by [http://www.promega.com/figures/popup.asp?fn=0313va Promega]]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgl2-b.txt Promega]
|-
!{{table heading style}} |Features
|
Lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences.

|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650339 X65323]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.D47176b06a95f8&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 
==Notes==

Lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGL2%2DBasic+Vector&description%5Ftext=pGL2%2DBasic+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==

<pre>
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC

51 TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA

101 AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA

151 CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT

201 TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA

251 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT

301 CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT

351 GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT

401 ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA

451 GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA

501 ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC

551 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT

601 TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT

651 TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG

701 CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT

751 ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC

801 ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG

851 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA

901 TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT

951 TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT

1001 TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA

1051 GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT

1101 CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC

1151 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT

1201 CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT

1251 CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA

1301 ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC

1351 TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT

1401 TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT

1451 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT

1501 GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC

1551 GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG

1601 CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT

1651 CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC

1701 CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG

1751 ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT

1801 AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC

1851 TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG

1901 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA

1951 GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT

2001 TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA

2051 CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT

2101 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA

2151 ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT

2201 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA

2251 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT

2301 ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA

2351 ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA

2401 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT

2451 AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA

2501 GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA

2551 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG

2601 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT

2651 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG

2701 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCCGTCGAC

2751 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG

2801 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT

2851 CGTAGGACAG GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG

2901 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC

2951 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT

3001 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG

3051 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG

3101 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

3151 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

3201 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA

3251 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC

3301 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC

3351 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT

3401 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

3451 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA

3501 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT

3551 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT

3601 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT

3651 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG

3701 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

3751 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA

3801 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC

3851 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA

3901 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA

3951 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC

4001 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA

4051 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT

4101 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG

4151 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC

4201 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT

4251 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT

4301 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT

4351 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG

4401 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC

4451 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC

4501 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC

4551 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC

4601 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG

4651 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA

4701 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT

4751 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC

4801 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT

4851 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT

4901 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG

4951 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC

5001 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC

5051 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA

5101 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC

5151 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA

5201 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA

5251 CGCTTACAAT TTGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG

5301 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCCCAAGC TACCATGATA

5351 AGTAAGTAAT ATTAAGGTAC GTGGAGGTTT TACTTGCTTT AAAAAACCTC

5401 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT

5451 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA

5501 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG

5551 TCCAAACTCA TCAATGTATC TTATGGTACT GTAACTGAGC TAACATAA

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGEM-13Zf(+)

2008-04-04T06:17:19Z

Mattnat08: /* References */

==At a Glance==
[[Image:#pGEM-13Zf(+).gif|thumb|right|300px|pGEM-13Zf(+), image provided by [http://www.promega.com/figures/popup.asp?fn=0292va Promega]]]
[[Image:PGEM-13Zf(+).jpg|thumb|right|450px|pGEM-13Zf(+), Key: Red = marker, green = origin, cyan = MCS, magenta = promoters]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgem13zp.txt Promega]
|-
!{{table heading style}} |Features
|
T7 RNA polymerase transcription initiation site

SP6 RNA polymerase initiation site

T7 RNA promoter

SP6 RNA promoter

multiple cloning region

''lac''Z start codon

''lac'' operon sequences

''lac''1 operator

beta-lactamase coding region

phage f1 region

binding site of pUC/M13 forward sequencing primer

binding site of pUC/M13 reverse sequencing primer
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65315 X65315]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.W4716684319347&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGEM%2D13Zf%28%2B%29+Vector&description%5Ftext=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D13Zf%28%2B%29+Vector Promega]

==References==

<ref name='PMID:2985470 '/>
<references/>

==Sequence==
<pre>
1 GGGCGAATTG GCGGCCGCTC CCTGGCCGAC TTGGCCCAAG CTTGAGTATT
51 CTATAGTGTC ACCTAAATAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC
101 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
151 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
201 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
251 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
301 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
351 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
451 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
501 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
551 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
601 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
651 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
751 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
801 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
851 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
901 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
951 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
1001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
1051 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
1101 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
1151 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
1201 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
1251 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
1301 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
1351 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
1401 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
1451 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
1501 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
1551 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
1601 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCC
1651 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
1701 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
1751 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
1801 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
1851 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
1901 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
1951 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
2001 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
2051 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
2101 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
2151 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
2201 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA
2251 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC
2301 TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA
2351 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC
2401 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT
2451 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG
2501 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT
2551 AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT
2601 CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA
2651 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC
2701 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC
2751 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG
2801 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT
2851 TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA
2901 AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG
2951 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC
3001 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
3051 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT
3101 TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
3151 GCCAGTGAAT TGTAATACGA CTCACTATA
</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGEM-13Zf(+)

2008-04-04T06:16:18Z

Mattnat08: edited by Mattnat08 via TableEdit

==At a Glance==
[[Image:#pGEM-13Zf(+).gif|thumb|right|300px|pGEM-13Zf(+), image provided by [http://www.promega.com/figures/popup.asp?fn=0292va Promega]]]
[[Image:PGEM-13Zf(+).jpg|thumb|right|450px|pGEM-13Zf(+), Key: Red = marker, green = origin, cyan = MCS, magenta = promoters]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgem13zp.txt Promega]
|-
!{{table heading style}} |Features
|
T7 RNA polymerase transcription initiation site

SP6 RNA polymerase initiation site

T7 RNA promoter

SP6 RNA promoter

multiple cloning region

''lac''Z start codon

''lac'' operon sequences

''lac''1 operator

beta-lactamase coding region

phage f1 region

binding site of pUC/M13 forward sequencing primer

binding site of pUC/M13 reverse sequencing primer
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65315 X65315]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.W4716684319347&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGEM%2D13Zf%28%2B%29+Vector&description%5Ftext=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D13Zf%28%2B%29+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GGGCGAATTG GCGGCCGCTC CCTGGCCGAC TTGGCCCAAG CTTGAGTATT
51 CTATAGTGTC ACCTAAATAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC
101 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
151 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
201 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
251 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
301 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
351 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
451 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
501 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
551 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
601 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
651 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
751 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
801 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
851 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
901 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
951 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
1001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
1051 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
1101 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
1151 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
1201 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
1251 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
1301 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
1351 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
1401 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
1451 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
1501 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
1551 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
1601 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCC
1651 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
1701 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
1751 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
1801 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
1851 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
1901 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
1951 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
2001 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
2051 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
2101 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
2151 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
2201 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA
2251 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC
2301 TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA
2351 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC
2401 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT
2451 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG
2501 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT
2551 AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT
2601 CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA
2651 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC
2701 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC
2751 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG
2801 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT
2851 TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA
2901 AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG
2951 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC
3001 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
3051 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT
3101 TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
3151 GCCAGTGAAT TGTAATACGA CTCACTATA
</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGEM-13Zf(+)

2008-04-04T06:09:29Z

Mattnat08: /* Notes */

==At a Glance==
[[Image:#pGEM-13Zf(+).gif|thumb|right|300px|pGEM-13Zf(+), image provided by [http://www.promega.com/figures/popup.asp?fn=0292va Promega]]]
[[Image:PGEM-13Zf(+).jpg|thumb|right|450px|pGEM-13Zf(+), Key: Red = marker, green = origin, cyan = MCS, magenta = promoters]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgem13zp.txt Promega]
|-
!{{table heading style}} |Features
|
The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109).
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65315 X65315]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.W4716684319347&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
The plasmid contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGEM%2D13Zf%28%2B%29+Vector&description%5Ftext=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D13Zf%28%2B%29+Vector Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GGGCGAATTG GCGGCCGCTC CCTGGCCGAC TTGGCCCAAG CTTGAGTATT
51 CTATAGTGTC ACCTAAATAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC
101 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
151 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
201 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA
251 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
301 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
351 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
451 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
501 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
551 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
601 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
651 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
751 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
801 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
851 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
901 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
951 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
1001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
1051 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
1101 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
1151 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
1201 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
1251 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
1301 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
1351 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
1401 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
1451 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
1501 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
1551 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
1601 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCC
1651 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
1701 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
1751 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
1801 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
1851 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
1901 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
1951 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
2001 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
2051 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
2101 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
2151 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
2201 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA
2251 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC
2301 TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA
2351 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC
2401 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT
2451 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG
2501 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT
2551 AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT
2601 CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA
2651 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC
2701 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC
2751 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG
2801 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT
2851 TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA
2901 AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG
2951 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC
3001 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
3051 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT
3101 TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
3151 GCCAGTGAAT TGTAATACGA CTCACTATA
</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGEM-11Zf(-)

2008-04-04T06:07:46Z

Mattnat08: /* References */

==At a Glance==
[[Image:#pGEM-11Zf(-).gif|thumb|right|300px|pGEM-11Zf(-), image provided by [http://www.promega.com/figures/popup.asp?fn=0290vb Promega]]]
[[Image:PGEM-11Zf-.jpg|thumb|right|400px|pGEM-11Zf(-), Key: red=marker, green=ori, magenta=promoter, cyan=MCS]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgem11zm.txt Promega]
|-
!{{table heading style}} |Features
|
T7 RNA Polymerase transcription initiation site

SP6 RNA polymerase transcription initiation site

T& RNA polymerase promoter

SP6 RNA polymerase promoter

Multiple cloning region

''lac''Z start codon

''lac'' operon sequences

''lac'' operator

beta-lactamase coding region
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=4495058 X65314]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.U47165dbeb5f4c&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGEM%2D11Zf%28%2B%2F%2D%29+Vectors&description%5Ftext=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D11Zf%28%2B%2F%26%23x2013%3B%29+Vectors Promega]

==References==

<ref name='PMID:2985470'/>
<references/>

==Sequence==
<pre>
1 GGGCGAATTG GCCAAGTCGG CCGAGCTCGA ATTCGTCGAC CTCGAGGGAT

51 CCGGGCCCTC TAGATGCGGC CGCATGCATA AGCTTGAGTA TTCTATAGTG

101 TCACCTAAAT AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA

151 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG

201 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT

251 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT

301 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT

351 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG

401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG

451 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG

501 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC

551 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA

601 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC

651 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC

701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT

751 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT

801 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC

851 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA

901 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT

951 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA

1001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA

1051 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC

1101 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA

1151 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT

1201 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA

1251 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT

1301 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG

1351 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA

1401 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC

1451 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG

1501 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA

1551 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT

1601 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA

1651 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC

1701 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT

1751 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA

1801 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC

1851 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC

1901 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA

1951 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG

2001 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC

2051 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA

2101 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA

2151 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT

2201 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC

2251 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT

2301 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT

2351 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG

2401 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT

2451 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC

2501 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC

2551 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGACGCG CCCTGTAGCG

2601 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA

2651 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT

2701 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT

2751 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT

2801 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG

2851 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA

2901 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG

2951 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA

3001 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TCCATTCGCC

3051 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC

3101 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG

3151 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA

3201 ATTGTAATAC GACTCACTAT A

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGEM-11Zf(-)

2008-04-04T06:05:50Z

Mattnat08: edited by Mattnat08 via TableEdit

==At a Glance==
[[Image:#pGEM-11Zf(-).gif|thumb|right|300px|pGEM-11Zf(-), image provided by [http://www.promega.com/figures/popup.asp?fn=0290vb Promega]]]
[[Image:PGEM-11Zf-.jpg|thumb|right|400px|pGEM-11Zf(-), Key: red=marker, green=ori, magenta=promoter, cyan=MCS]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgem11zm.txt Promega]
|-
!{{table heading style}} |Features
|
T7 RNA Polymerase transcription initiation site

SP6 RNA polymerase transcription initiation site

T& RNA polymerase promoter

SP6 RNA polymerase promoter

Multiple cloning region

''lac''Z start codon

''lac'' operon sequences

''lac'' operator

beta-lactamase coding region
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=4495058 X65314]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.U47165dbeb5f4c&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGEM%2D11Zf%28%2B%2F%2D%29+Vectors&description%5Ftext=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D11Zf%28%2B%2F%26%23x2013%3B%29+Vectors Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GGGCGAATTG GCCAAGTCGG CCGAGCTCGA ATTCGTCGAC CTCGAGGGAT

51 CCGGGCCCTC TAGATGCGGC CGCATGCATA AGCTTGAGTA TTCTATAGTG

101 TCACCTAAAT AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA

151 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG

201 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT

251 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT

301 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT

351 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG

401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG

451 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG

501 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC

551 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA

601 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC

651 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC

701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT

751 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT

801 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC

851 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA

901 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT

951 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA

1001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA

1051 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC

1101 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA

1151 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT

1201 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA

1251 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT

1301 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG

1351 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA

1401 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC

1451 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG

1501 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA

1551 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT

1601 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA

1651 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC

1701 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT

1751 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA

1801 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC

1851 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC

1901 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA

1951 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG

2001 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC

2051 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA

2101 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA

2151 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT

2201 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC

2251 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT

2301 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT

2351 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG

2401 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT

2451 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC

2501 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC

2551 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGACGCG CCCTGTAGCG

2601 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA

2651 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT

2701 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT

2751 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT

2801 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG

2851 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA

2901 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG

2951 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA

3001 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TCCATTCGCC

3051 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC

3101 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG

3151 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA

3201 ATTGTAATAC GACTCACTAT A

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGEM-11Zf(-)

2008-04-04T05:57:59Z

Mattnat08: /* Notes */

==At a Glance==
[[Image:#pGEM-11Zf(-).gif|thumb|right|300px|pGEM-11Zf(-), image provided by [http://www.promega.com/figures/popup.asp?fn=0290vb Promega]]]
[[Image:PGEM-11Zf-.jpg|thumb|right|400px|pGEM-11Zf(-), Key: red=marker, green=ori, magenta=promoter, cyan=MCS]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgem11zm.txt Promega]
|-
!{{table heading style}} |Features
|
These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase (1). Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109).
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=4495058 X65314]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.U47165dbeb5f4c&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGEM%2D11Zf%28%2B%2F%2D%29+Vectors&description%5Ftext=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D11Zf%28%2B%2F%26%23x2013%3B%29+Vectors Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GGGCGAATTG GCCAAGTCGG CCGAGCTCGA ATTCGTCGAC CTCGAGGGAT

51 CCGGGCCCTC TAGATGCGGC CGCATGCATA AGCTTGAGTA TTCTATAGTG

101 TCACCTAAAT AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA

151 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG

201 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT

251 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT

301 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT

351 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG

401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG

451 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG

501 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC

551 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA

601 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC

651 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC

701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT

751 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT

801 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC

851 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA

901 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT

951 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA

1001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA

1051 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC

1101 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA

1151 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT

1201 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA

1251 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT

1301 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG

1351 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA

1401 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC

1451 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG

1501 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA

1551 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT

1601 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA

1651 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC

1701 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT

1751 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA

1801 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC

1851 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC

1901 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA

1951 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG

2001 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC

2051 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA

2101 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA

2151 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT

2201 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC

2251 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT

2301 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT

2351 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG

2401 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT

2451 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC

2501 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC

2551 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGACGCG CCCTGTAGCG

2601 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA

2651 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT

2701 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT

2751 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT

2801 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG

2851 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA

2901 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG

2951 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA

3001 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TCCATTCGCC

3051 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC

3101 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG

3151 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA

3201 ATTGTAATAC GACTCACTAT A

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGEM-11Zf(+)

2008-04-04T05:55:13Z

Mattnat08: /* References */

==At a Glance==
[[Image:#pGEM-11Zf(+).gif|thumb|right|300px|pGEM-11Zf(+), image provided by [http://www.promega.com/figures/popup.asp?fn=0290vc Promega]]]
[[Image:PGEM-11Zf+.jpg|thumb|right|400px|pGEM-11Zf(+), Key: red = amp marker, green = ori, magenta = promoter region, cyan = MCS]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgem11zp.txt Promega]
|-
!{{table heading style}} |Features
|
T7 RNA polymerase transcription initiation site

SP6 RNA Polymerase transcription initiation site

''lac''Z start codon

''lac'' operon sequences

''lac operator

beta-lactamase coding region
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65313 X65313]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.D47165da3d0d8c&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGEM%2D11Zf%28%2B%2F%2D%29+Vectors&description%5Ftext=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D11Zf%28%2B%2F%26%23x2013%3B%29+Vectors Promega]

==References==

<ref name='PMID:2985470 '/>
<references/>

==Sequence==
<pre>
1 GGGCGAATTG GCCAAGTCGG CCGAGCTCGA ATTCGTCGAC CTCGAGGGAT

51 CCGGGCCCTC TAGATGCGGC CGCATGCATA AGCTTGAGTA TTCTATAGTG

101 TCACCTAAAT AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA

151 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG

201 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT

251 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT

301 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT

351 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG

401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG

451 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG

501 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC

551 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA

601 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC

651 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC

701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT

751 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT

801 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC

851 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA

901 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT

951 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA

1001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA

1051 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC

1101 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA

1151 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT

1201 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA

1251 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT

1301 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG

1351 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA

1401 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC

1451 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG

1501 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA

1551 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT

1601 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA

1651 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC

1701 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT

1751 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA

1801 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC

1851 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC

1901 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA

1951 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG

2001 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC

2051 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA

2101 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA

2151 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT

2201 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC

2251 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT

2301 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT

2351 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG

2401 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT

2451 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC

2501 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC

2551 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGAAATT GTAAGCGTTA

2601 ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT

2651 AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC

2701 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA

2751 AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT

2801 GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG

2851 CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT

2901 GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA

2951 GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC

3001 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCATTCGCC

3051 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC

3101 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG

3151 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA

3201 ATTGTAATAC GACTCACTAT A

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__

pGEM-11Zf(+)

2008-04-04T05:52:54Z

Mattnat08: edited by Mattnat08 via TableEdit

==At a Glance==
[[Image:#pGEM-11Zf(+).gif|thumb|right|300px|pGEM-11Zf(+), image provided by [http://www.promega.com/figures/popup.asp?fn=0290vc Promega]]]
[[Image:PGEM-11Zf+.jpg|thumb|right|400px|pGEM-11Zf(+), Key: red = amp marker, green = ori, magenta = promoter region, cyan = MCS]]
<protect>

{|{{Prettytable}} width='30%' align=left
|-
!{{table heading style}} |Type
|
Cloning
|-
!{{table heading style}} |Origin of Replication
|
f1
|-
!{{table heading style}} |Copy #
|

|-
!{{table heading style}} |Markers
|
Ampicillin
|-
!{{table heading style}} |Link to Sequence
|
[http://www.promega.com/vectors/pgem11zp.txt Promega]
|-
!{{table heading style}} |Features
|
T7 RNA polymerase transcription initiation site

SP6 RNA Polymerase transcription initiation site

''lac''Z start codon

''lac'' operon sequences

''lac operator

beta-lactamase coding region
|-
!{{table heading style}} |Accession Number
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=X65313 X65313]
|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.D47165da3d0d8c&page=0&pagename={{FULLPAGENAMEE}} edit table]
|}
</protect>
 
 

==Notes==
These plasmids contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains.

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGEM%2D11Zf%28%2B%2F%2D%29+Vectors&description%5Ftext=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D11Zf%28%2B%2F%26%23x2013%3B%29+Vectors Promega]

==References==
{{RefHelp}}
<references/>

==Sequence==
<pre>
1 GGGCGAATTG GCCAAGTCGG CCGAGCTCGA ATTCGTCGAC CTCGAGGGAT

51 CCGGGCCCTC TAGATGCGGC CGCATGCATA AGCTTGAGTA TTCTATAGTG

101 TCACCTAAAT AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA

151 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG

201 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT

251 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT

301 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT

351 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG

401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG

451 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG

501 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC

551 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA

601 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC

651 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC

701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT

751 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT

801 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC

851 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA

901 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT

951 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA

1001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA

1051 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC

1101 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA

1151 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT

1201 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA

1251 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT

1301 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG

1351 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA

1401 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC

1451 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG

1501 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA

1551 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT

1601 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA

1651 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC

1701 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT

1751 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA

1801 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC

1851 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC

1901 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA

1951 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG

2001 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC

2051 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA

2101 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA

2151 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT

2201 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC

2251 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT

2301 ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT

2351 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG

2401 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT

2451 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC

2501 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC

2551 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGAAATT GTAAGCGTTA

2601 ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT

2651 AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC

2701 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA

2751 AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT

2801 GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG

2851 CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT

2901 GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA

2951 GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC

3001 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCATTCGCC

3051 ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC

3101 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG

3151 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA

3201 ATTGTAATAC GACTCACTAT A

</pre>

[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__