PMID:2670887

2007-08-10T04:40:13Z

Lbrd: New PMID: Page!

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<span style = 'font-size: large'> '''Kiino, DR and Rothman-Denes, LB''' (1989) Genetic analysis of bacteriophage N4 adsorption.''J. Bacteriol.'' '''171''':4595-602</span>

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|{{TableHeadingColor}} |'''Abstract''' || We isolated six mutants of Escherichia coli K-12 that were defective in bacteriophage N4 adsorption. We mapped the mutations to four loci designated nfrA through nfrD (N four resistance). nfrA and nfrB were tightly linked to each other and were mapped to min 12 of the E. coli linkage map. nfrC was mapped to min 85, and nfrD was mapped between min 44 and 58. We isolated a clone carrying both nfrA and nfrB and identified its gene products through maxicell analysis of plasmid subclones. The nfrA gene product was an outer membrane protein of 96,000 apparent molecular weight, whereas nfrB encoded an inner-membrane protein of 69,500 apparent molecular weight. The nfrB1 mutation did not affect the export of the nfrA gene product to the outer membrane and did not affect the alkaline phosphatase activity of an nfrA-phoA fusion. We propose that nfrA encodes the structural receptor for N4 and that the nfrB gene product may be required for irreversible adsorption and injection of the phage genome and virion-encapsulated RNA polymerase through the inner membrane.
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2670887 PubMed]
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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
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<references/>

nfrA:Gene

2007-08-10T04:39:11Z

Lbrd: /* Notes */

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{{Tab|Quickview|nfrA}}{{Tab On|Gene|nfrA}}{{Tab|Gene Product(s)|nfrA}}{{Tab|Expression|nfrA}}{{Tab|Evolution|nfrA}}{{Tab|On One Page|nfrA}}{{TabsBottom}}
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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
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==Nomenclature==
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!{{table_heading_style}} |Standard name
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''nfrA''
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!{{table_heading_style}} |Mnemonic
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!{{table_heading_style}} |Synonyms
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''ECK0560, b0568, JW0557''<ref name='LIB:Riley_2006'/>
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===Notes===

==Location(s) and DNA Sequence==
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{|class = 'sortable' {{Prettytable}} id='128291'
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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MG1655
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12.66 minutes 
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MG1655: 590177..587205<br />{{GBrowseFigureByCoord|586205|591177|ecoli|MG1655}}
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*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11740 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:586205..591177 GBrowse(MG1655)]

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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:587205..590177&plugin_action=Go FASTA Dump]
*[http://biocyc.org/ECOLI/sequence?object=EG11740 EcoCyc Sequence Dump]
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W3110
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W3110: 590177..587205<br />{{GBrowseFigureByCoord|586205|591177|ecoli_W3110|W3110}}
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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:586205..591177 GBrowse(W3110)]
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*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:587205..590177&plugin_action=Go FASTA Dump]
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===Notes===

==Sequence Features==
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!Feature type!!Location!!Description
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====Notes====

==Alleles and Phenotypes==
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{|class = 'sortable' {{Prettytable}} id='128293'
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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ΔnfrA (Keio:JW0557)
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deletion
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deletion
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[[PMID:16738554]]<ref name=PMID:16738554/>
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
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===Notes===
The ''nfr'' genes were defined by phage N4 resistant mutants <ref name='PMID: 2670887'/>

==Genetic Resources==
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{|class = 'sortable' {{Prettytable}} id='128294'
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!Resource!!Resource Type!!Source!!Notes/Reference
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JW0557
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Plasmid clone
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
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[[PMID:16769691]]<ref name=PMID:16769691/>

Status:Clone OK

Primer 1:GCCAAGGAGAATAACCTTAATCG

Primer 2:CCCCAGTGCACTCCAATGGTGAG
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[[purK:Gene|purK79::Tn10]]
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Linked marker
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CAG12171 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39380 CGSC7342]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 24% <ref name='LIB:Nichols_linked_Tn'/>

Synonyms:purE79::Tn10
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[[dsbG:Gene|dsbG601::Tn10]]
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Linked marker
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CAG12149 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39387 CGSC7346]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 11% <ref name='LIB:Nichols_linked_Tn'/>

Synonyms:zbd-601::Tn10, zbe-601::Tn10
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===Notes===

==Accessions in Other Databases==
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{|class = 'sortable' {{Prettytable}} id='128295'
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!Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11740 EcoCyc:EG11740]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1691 EchoBASE:EB1691]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b0568 EcoliGenExpDB:b0568]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C0654 ECOL199310CYC:C0654]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG11740 EcoGene:EG11740]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=NFRA ECOO157CYC:NFRA]
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|-
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/31154 CGSC:31154]
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===Notes===

==Links==
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{{Links_help}}

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==References==
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{{RefHelp}}
<references/>
__NOTOC__
</protect></noinclude>
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]

EcoliWiki - User contributions [en]

PMID:2670887

nfrA:Gene