EcoliWiki - User contributions [en]

mscL:Gene

2013-11-13T19:26:54Z

Jtownley: Table edited by Jtownley via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|mscL}}{{Tab On|Gene|mscL}}{{Tab|Gene Product(s)|mscL}}{{Tab|Expression|mscL}}{{Tab|Evolution|mscL}}{{Tab|On One Page|mscL}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="J46a9f23915d43" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''mscL''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Mechanosensitive channel
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK3277, b3291, JW3252, yhdC''<ref name='LIB:Riley_2006'/>, stkA, yhdC''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.J46a9f23915d43&page=17129&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
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<protect>

{{Gene_location_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S46a9f239962db" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
74.06 minutes 
|
MG1655: 3436046..3436456 <gbrowseImage>
name=NC_000913:3436046..3436456
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11180 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|3435046|3437456|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|3436046|3436456|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11180 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 3365929..3366339 <gbrowseImage>
name=NC_012967:3365929..3366339
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|3365929|3366339|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3365929|3366339|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 3323203..3323613 <gbrowseImage>
name=NC_012759:3323203..3323613
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|3323203|3323613|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3323203|3323613|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4202392..4201982 <gbrowseImage>
name=NC_007779:4201982..4202392
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|NC_007779|4200982|4203392|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|4201982|4202392|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 3533791..3534201 <gbrowseImage>
name=NC_010473:3533791..3534201
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|3532971|3535021|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|3532971|3535021|FASTA Dump}}

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|}


</protect>
===Notes===

==Sequence Features==
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{{Gene_sequence_features_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="T46a9f23a56149" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
3436046
|
Edman degradation
|
PMID:7511799 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.T46a9f23a56149&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a9f23a9ebac" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔmscL (Keio:JW3252)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] [http://cgsc2.biology.yale.edu/Strain.php?ID=108734 CGSC10453]<ref name='LIB:cgsc'/>
|

|-
|
''ΔmscL727::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:101954|CGSC:101954]]
|

|-
|
mscL-K31C
|
-->TGC
|
K31C
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See Table 1
|-
|
''mscL-K31R''
|
-->AGG
|
K31R
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental:AW405
|
See table 1
|-
|
''mscL-K31E''
|
-->GAG
|
K31E
|
Sensitivity to
|
Activation pressure lowered by 18.3 mmHg
|
PMID:9635742
|
Parental:AW405
|
See table 4
|-
|
''mscL-Q56P''
|
-->CCT
|
Q56P
|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental:AW405
|
see table 1
|-
|
''mscL-Q56C''
|
-->TGC
|
Q56C
|
Conductance
|
Increased channel open time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56F''
|
-->TTT
|
Q56F
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56W''
|
-->TGG
|
Q56W
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
see table 1
|-
|
''mscL-Q56Y''
|
-->TAC
|
Q56Y
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56L''
|
-->TTA
|
Q56L
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56R''
|
-->CGT
|
Q56R
|
Sensitivity to
|
Activation pressure lowered by 17.9 mmHg
|
PMID:9635742
|
Parental: AW405
|
See table 4
|-
|
''mscLΔ2-4''
|
Deletion
|

|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-ΔQ56''
|
Deletion
|

|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscLΔ110-136''
|
Deletion
|

|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1

|-
|
''mscL-Q56H''
|
-->CAT
|
Q56H
|
Sensitivity to
|
Increased sensitivity to pressure at pH 5 (activation pressure lowered by 18.6 mmHg) and increased open channel time at pH 7.5.
|
PMID:8876191
PMID:9635742
|
Parental: AW405
|
See table 1 and table 4
|-
|
''mscL-K31D''
|
-->GAT
|
K31D
|
Growth Phenotype
|
Inhibits growth
|
PMID:9405414
|

|
See figure 1
|-
|
''mscL-K31E''
|

|
K31E
|
Growth Phenotype
|
Inhibits growth
|
PMID:9405414
|

|
See figure 1
|-
|
''mscLΔ104-136''
|
Deletion
|

|
Conductance
|
Failed to exhibit functional channel
|
PMID:9141355
|

|
See table 2
|-
|
''mscLΔEV''
|
Internal deletion (Δ41-61)
|

|
Conductance
|
Failed to show functional channels
|
PMID:9141355
|

|
See table 2
|-
|
''mscLNBE''
|
Deletion (Δ1-8)
|

|
Conductance
|
Decreased conductance
|
PMID:9141355
|

|
See table 2
|-
|
''mscL-G22R''
|

|
G22R
|
Growth Phenotype
|
Growth inhibited and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22K''
|

|
G22K
|
Growth Phenotype
|
Growth inhibited and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22D''
|

|
G22D
|
Growth Phenotype
|
Growth inhibited and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.

|-
|
''mscL-G22E''
|

|
G22D
|
Growth Phenotype
|
Growth inhibited and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22S''
|

|
G22S
|
Growth Phenotype
|
Growth decreased and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22Y''
|

|
G22Y
|
Growth Phenotype
|
Growth decreased
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1
|-
|
''mscL-G22Q''
|

|
G22Q
|
Growth Phenotype
|
Growth decreased and pressure threshold lowered.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
mscL-G22T''
|

|
G22T
|
Growth Phenotype
|
Growth decreased and pressure threshold lowered
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22V''
|

|
G22V
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1
|-
|
''mscL-G22L''
|

|
G22L
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22W''
|

|
G22W
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22F''
|

|
G22F
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G11I''
|

|
G22I
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22M''
|

|
G22M
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22C''
|

|
G22C
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.
|-
|
''mscL-G22P''
|

|
G22P
|
Resistant to
|
Pressure threshold increased. No change in growth.
|
PMID:10512816
|
Parental plasmid: pb10b
|
See figure 1a and table 1.

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|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J4e04bfa3717ad" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.17129.J4e04bfa3717ad&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9f23b5eb85" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW3252
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCAGCATTATTAAAGAATTTCG

Primer 2:CCAGAGCGGTTATTCTGCTCTTT
|-
|
[[smg:Gene|smg-3082::Tn10]]
|
Linked marker
|
CAG12071 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40240 CGSC7444]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 78% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
|-
|
[[yhfT:Gene|zhf-3084::Tn10]]
|
Linked marker
|
CAG18456 = [http://cgsc2.biology.yale.edu/Strain.php?ID=61450 CGSC7576]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 2% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.B46a9f23b5eb85&page=17129&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="N46a9f23c0d3d3" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[[EcoCyc]]
|
EcoCyc:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[RegulonDB]]
|
RegulonDB:ECK120001162
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947787 GeneID:947787]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EchoBASE]]
|
EchoBASE:EB1167
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[ASAP]]
|
ASAP:ABE-0010790
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]

|- class="tableEdit_footer"
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<protect></protect>
===Notes===

==Links==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46a70d7f13e9e" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.P46a70d7f13e9e&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:msc genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

mscL:Gene

2013-11-12T05:46:44Z

Jtownley: Table edited by Jtownley via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|mscL}}{{Tab On|Gene|mscL}}{{Tab|Gene Product(s)|mscL}}{{Tab|Expression|mscL}}{{Tab|Evolution|mscL}}{{Tab|On One Page|mscL}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="J46a9f23915d43" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''mscL''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Mechanosensitive channel
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK3277, b3291, JW3252, yhdC''<ref name='LIB:Riley_2006'/>, stkA, yhdC''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.J46a9f23915d43&page=17129&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S46a9f239962db" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
74.06 minutes 
|
MG1655: 3436046..3436456 <gbrowseImage>
name=NC_000913:3436046..3436456
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11180 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|3435046|3437456|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|3436046|3436456|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11180 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 3365929..3366339 <gbrowseImage>
name=NC_012967:3365929..3366339
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|3365929|3366339|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3365929|3366339|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 3323203..3323613 <gbrowseImage>
name=NC_012759:3323203..3323613
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|3323203|3323613|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3323203|3323613|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4202392..4201982 <gbrowseImage>
name=NC_007779:4201982..4202392
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|NC_007779|4200982|4203392|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|4201982|4202392|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 3533791..3534201 <gbrowseImage>
name=NC_010473:3533791..3534201
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|3532971|3535021|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|3532971|3535021|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.S46a9f239962db&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="T46a9f23a56149" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
3436046
|
Edman degradation
|
PMID:7511799 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.T46a9f23a56149&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a9f23a9ebac" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔmscL (Keio:JW3252)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] [http://cgsc2.biology.yale.edu/Strain.php?ID=108734 CGSC10453]<ref name='LIB:cgsc'/>
|

|-
|
''ΔmscL727::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:101954|CGSC:101954]]
|

|-
|
mscL-K31C
|
-->TGC
|
K31C
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See Table 1
|-
|
''mscL-K31R''
|
-->AGG
|
K31R
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental:AW405
|
See table 1
|-
|
''mscL-K31E''
|
-->GAG
|
K31E
|
Sensitivity to
|
Activation pressure lowered by 18.3 mmHg
|
PMID:9635742
|
Parental:AW405
|
See table 4
|-
|
''mscL-Q56P''
|
-->CCT
|
Q56P
|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental:AW405
|
see table 1
|-
|
''mscL-Q56C''
|
-->TGC
|
Q56C
|
Conductance
|
Increased channel open time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56F''
|
-->TTT
|
Q56F
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56W''
|
-->TGG
|
Q56W
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
see table 1
|-
|
''mscL-Q56Y''
|
-->TAC
|
Q56Y
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56L''
|
-->TTA
|
Q56L
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56R''
|
-->CGT
|
Q56R
|
Sensitivity to
|
Activation pressure lowered by 17.9 mmHg
|
PMID:9635742
|
Parental: AW405
|
See table 4
|-
|
''mscLΔ2-4''
|
Deletion
|

|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-ΔQ56''
|
Deletion
|

|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscLΔ110-136''
|
Deletion
|

|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1

|-
|
''mscL-Q56H''
|
-->CAT
|
Q56H
|
Sensitivity to
|
Increased sensitivity to pressure at pH 5 (activation pressure lowered by 18.6 mmHg) and increased open channel time at pH 7.5.
|
PMID:8876191
PMID:9635742
|
Parental: AW405
|
See table 1 and table 4
|-
|
''mscL-K31D''
|
-->GAT
|
K31D
|
Growth Phenotype
|
Inhibits growth
|
PMID:9405414
|

|
See figure 1
|-
|
''mscL-K31E''
|

|
K31E
|
Growth Phenotype
|
Inhibits growth
|
PMID:9405414
|

|
See figure 1
|-
|
''mscLΔ104-136''
|
Deletion
|

|
Conductance
|
Failed to exhibit functional channel
|
PMID:9141355
|

|
See table 2
|-
|
''mscLΔEV''
|
Internal deletion (Δ41-61)
|

|
Conductance
|
Failed to show functional channels
|
PMID:9141355
|

|
See table 2
|-
|
''mscLNBE''
|
Deletion (Δ1-8)
|

|
Conductance
|
Decreased conductance
|
PMID:9141355
|

|
See table 2

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.U46a9f23a9ebac&page=17129&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J4e04bfa3717ad" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.17129.J4e04bfa3717ad&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9f23b5eb85" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW3252
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCAGCATTATTAAAGAATTTCG

Primer 2:CCAGAGCGGTTATTCTGCTCTTT
|-
|
[[smg:Gene|smg-3082::Tn10]]
|
Linked marker
|
CAG12071 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40240 CGSC7444]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 78% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
|-
|
[[yhfT:Gene|zhf-3084::Tn10]]
|
Linked marker
|
CAG18456 = [http://cgsc2.biology.yale.edu/Strain.php?ID=61450 CGSC7576]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 2% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.B46a9f23b5eb85&page=17129&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="N46a9f23c0d3d3" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[[EcoCyc]]
|
EcoCyc:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[RegulonDB]]
|
RegulonDB:ECK120001162
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947787 GeneID:947787]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EchoBASE]]
|
EchoBASE:EB1167
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[ASAP]]
|
ASAP:ABE-0010790
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.N46a9f23c0d3d3&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46a70d7f13e9e" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.P46a70d7f13e9e&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:msc genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

mscL:Gene

2013-11-06T19:24:59Z

Jtownley: Table edited by Jtownley via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|mscL}}{{Tab On|Gene|mscL}}{{Tab|Gene Product(s)|mscL}}{{Tab|Expression|mscL}}{{Tab|Evolution|mscL}}{{Tab|On One Page|mscL}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="J46a9f23915d43" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''mscL''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Mechanosensitive channel
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK3277, b3291, JW3252, yhdC''<ref name='LIB:Riley_2006'/>, stkA, yhdC''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.J46a9f23915d43&page=17129&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S46a9f239962db" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
74.06 minutes 
|
MG1655: 3436046..3436456 <gbrowseImage>
name=NC_000913:3436046..3436456
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11180 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|3435046|3437456|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|3436046|3436456|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11180 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 3365929..3366339 <gbrowseImage>
name=NC_012967:3365929..3366339
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|3365929|3366339|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3365929|3366339|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 3323203..3323613 <gbrowseImage>
name=NC_012759:3323203..3323613
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|3323203|3323613|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3323203|3323613|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4202392..4201982 <gbrowseImage>
name=NC_007779:4201982..4202392
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|NC_007779|4200982|4203392|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|4201982|4202392|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 3533791..3534201 <gbrowseImage>
name=NC_010473:3533791..3534201
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|3532971|3535021|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|3532971|3535021|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.S46a9f239962db&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="T46a9f23a56149" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
3436046
|
Edman degradation
|
PMID:7511799 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.T46a9f23a56149&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a9f23a9ebac" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔmscL (Keio:JW3252)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] [http://cgsc2.biology.yale.edu/Strain.php?ID=108734 CGSC10453]<ref name='LIB:cgsc'/>
|

|-
|
''ΔmscL727::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:101954|CGSC:101954]]
|

|-
|
mscL-K31C
|
-->TGC
|
K31C
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See Table 1
|-
|
''mscL-K31R''
|
-->AGG
|
K31R
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental:AW405
|
See table 1
|-
|
''mscL-K31E''
|
-->GAG
|
K31E
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental:AW405
|
See table 1
|-
|
''mscL-Q56P''
|
-->CCT
|
Q56P
|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental:AW405
|
see table 1
|-
|
''mscL-Q56C''
|
-->TGC
|
Q56C
|
Conductance
|
Increased channel open time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56F''
|
-->TTT
|
Q56F
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56W''
|
-->TGG
|
Q56W
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
see table 1
|-
|
''mscL-Q56Y''
|
-->TAC
|
Q56Y
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56L''
|
-->TTA
|
Q56L
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56R''
|
-->CGT
|
Q56R
|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscLΔ2-4''
|
Deletion
|

|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-ΔQ56''
|
Deletion
|

|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscLΔ110-136''
|
Deletion
|

|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1

|-
|
''mscL-Q56H''
|
-->CAT
|
Q56H
|
Sensitivity to
|
Increased sensitivity to pressure at pH 5 and increased open channel time at pH 7.5.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-K31D''
|
-->GAT
|
K31D
|
Growth Phenotype
|
Inhibits growth
|
PMID:9405414
|

|
See figure 1
|-
|
''mscL-K31E''
|

|
K31E
|
Growth Phenotype
|
Inhibits growth
|
PMID:9405414
|

|
See figure 1
|-
|
''mscLΔ104-136''
|
Deletion
|

|
Conductance
|
Failed to exhibit functional channel
|
PMID:9141355
|

|
See table 2
|-
|
''mscLΔEV''
|
Internal deletion (Δ41-61)
|

|
Conductance
|
Failed to show functional channels
|
PMID:9141355
|

|
See table 2
|-
|
''mscLNBE''
|
Deletion (Δ1-8)
|

|
Conductance
|
Decreased conductance
|
PMID:9141355
|

|
See table 2

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<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J4e04bfa3717ad" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

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|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9f23b5eb85" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW3252
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCAGCATTATTAAAGAATTTCG

Primer 2:CCAGAGCGGTTATTCTGCTCTTT
|-
|
[[smg:Gene|smg-3082::Tn10]]
|
Linked marker
|
CAG12071 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40240 CGSC7444]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 78% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
|-
|
[[yhfT:Gene|zhf-3084::Tn10]]
|
Linked marker
|
CAG18456 = [http://cgsc2.biology.yale.edu/Strain.php?ID=61450 CGSC7576]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 2% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="N46a9f23c0d3d3" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[[EcoCyc]]
|
EcoCyc:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[RegulonDB]]
|
RegulonDB:ECK120001162
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947787 GeneID:947787]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EchoBASE]]
|
EchoBASE:EB1167
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[ASAP]]
|
ASAP:ABE-0010790
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]

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<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46a70d7f13e9e" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

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|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:msc genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

mscL:Gene Product(s)

2013-11-06T17:03:40Z

Jtownley: Table edited by Jtownley via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|mscL}}{{Tab|Gene|mscL}}{{Tab On|Gene Product(s)|mscL}}{{Tab|Expression|mscL}}{{Tab|Evolution|mscL}}{{Tab|On One Page|mscL}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==

{{Product_nomenclature_help}}
{| id="B46aadde390181" class=" tableEdit Product_nomenclature_table"

|-
!align=left {{table heading style}} |Standard name
||
MscL
|-
!align=left {{table heading style}} |Synonyms
||
''mechanosensitive channel<ref name='LIB:Riley_2006'/>, B3291<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YhdC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, MscL<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK3277, JW3252, stkA, yhdC, b3291''
|-
!align=left {{table heading style}} |Product description
||
MscL (Large Mechanosensitive Channel)<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Mechanosensitive channel protein, high conductance; homopentameric<ref name='LIB:EcoGene'/>
|-
!align=left {{table heading style}} |EC number (for enzymes)
||

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|}


<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> 
{{Product_GO_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="X46aadde4aec97" class=" tableEdit GO_table_product dataTable"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
GO:0005886
|
plasma membrane
|
GOA:hamap GO_REF:0000020
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00115
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0005886
|
plasma membrane
|
GOA:spkw SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0997
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0005886
|
plasma membrane
|
GOA:spkw SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-1003
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0006810
|
transport
|
GOA:hamap GO_REF:0000020
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00115
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0006810
|
transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001185
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0006810
|
transport
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR019823
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0006810
|
transport
|
GOA:spkw SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0813
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0006811
|
ion transport
|
GOA:spkw SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0406
|
P
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0016020
|
membrane
|
GOA:spkw SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0472
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0016020
|
membrane
|
PMID:16858726
|
IDA: Inferred from Direct Assay
|

|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0016021
|
integral to membrane
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001185
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0016021
|
integral to membrane
|
GOA:interpro GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR019823
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0016021
|
integral to membrane
|
GOA:spkw SP_KW:GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0812
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0016021
|
integral to membrane
|
GO_REF:0000023
|
IEA: Inferred from Electronic Annotation
|
SP_SL:SL-9909
|
C
|
Seeded from [[EcoCyc]] (v14.0)
|
complete
|-
|

|
GO:0016021
|
integral to membrane
|
PMID:8890153
|
IDA: Inferred from Direct Assay
|

|
C
|

|
complete
|-
|

|
GO:0006811
|
ion transport
|
PMID:7511799
|
IDA: Inferred from Direct Assay
|

|
P
|

|
complete

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<big>'''Interactions'''</big>

{{Product_interactions_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="D46aadde7e91c0" class=" tableEdit Product_interactions_table dataTable"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence

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</protect>
===Notes===

==Localization==

{{Product_localization_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Q46a86fbeac763" class=" tableEdit Product_localization_table"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
|-
|
plasma membrane
|
C-terminus localized in the cytoplasm with 2 predicted transmembrane domains
|
Daley et al. (2005) <ref name=PMID:15919996/>
|

|

|-
|
Inner Membrane
|

|
PMID:8890153
|
[http://www.york.ac.uk/res/thomas/Echolocation.cfm?EB_SubLocID=EB1167 EchoLocation:mscL]
|

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<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>

{{Product_physical_properties_help}}
{| id="P46aaddea03d90" class=" tableEdit Product_phys_prop_table"

|-
!align=left {{table heading style}} |Name
||

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=EG11180-MONOMER at EcoCyc]
<pre>MSIIKEFREF AMRGNVVDLA VGVIIGAAFG KIVSSLVADI IMPPLGLLIG GIDFKQFAVT
LRDAQGDIPA VVMHYGVFIQ NVFDFLIVAF AIFMAIKLIN KLNRKKEEPA AAPAPTKEEV
LLTEIRDLLK EQNNRS</pre>
|-
!align=left {{table heading style}} |Length
||
136
|-
!align=left {{table heading style}} |Mol. Wt
||
17 Da
|-
!align=left {{table heading style}} |pI
||
6.1 (calculated)
|-
!align=left {{table heading style}} |Extinction coefficient
||
0 (calc based on 0 Y, 0 W, and 0 C residues)

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|}


<big>'''Domains/Motifs/Modification Sites'''</big>
<table>
<tr align="left" valign="top">
<td>


{{Product_domains_motifs_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J46aaddea7b2ca" class=" tableEdit Product_motif_table dataTable"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes!!References
|-
|
Domain
|
3..132
|
[http://pfam.janelia.org/family/PF01741 PF01741 Large-conductance mechanosensitive channel, MscL]
|

|
PMID:19920124

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|}


</td>
<td>


<motif_map/>
{{TMHMM Picture|mscL}}
</td>
</tr>
</table>

<table><tr valign='top'><td>
<big>'''Structure'''</big> 

{{Product_structure_help}}
{| id="Q46a86fc04f613" class=" tableEdit Product_structure_table"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=mscL
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://www.proteinmodelportal.org/query/uniprot/P0A742 Protein Model Portal]
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0A742 PSI-PRED (P0A742)]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0A742 Genomes TO Protein (P0A742)] (GTOP)

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</td><td></protect>
==== Structure figures ====

<protect>
</td><tr><table></protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}

{{Product_resources_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46aaddeb4c726" class=" tableEdit Product_resource_table"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|- class="tableEdit_footer"
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<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="R46aaddebaede0" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/16131170 GI:16131170]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_417749.1 GeneID:947787]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0010790
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.uniprot.org/uniprot/P0A742 UniProtKB/Swiss-Prot:P0A742]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EcoCyc]]
|
EcoCyc:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947787 GeneID:947787]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[RegulonDB]]
|
RegulonDB:ECK120001162
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EchoBASE]]
|
EchoBASE:EB1167
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]

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<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a86fc3e2895" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

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<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
{{BacktoTop}}
[[Category:Proteins]]

mscL:Gene

2013-11-06T16:51:59Z

Jtownley: Table edited by Jtownley via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|mscL}}{{Tab On|Gene|mscL}}{{Tab|Gene Product(s)|mscL}}{{Tab|Expression|mscL}}{{Tab|Evolution|mscL}}{{Tab|On One Page|mscL}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="J46a9f23915d43" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''mscL''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Mechanosensitive channel
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK3277, b3291, JW3252, yhdC''<ref name='LIB:Riley_2006'/>, stkA, yhdC''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.J46a9f23915d43&page=17129&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S46a9f239962db" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
74.06 minutes 
|
MG1655: 3436046..3436456 <gbrowseImage>
name=NC_000913:3436046..3436456
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11180 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|3435046|3437456|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|3436046|3436456|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11180 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 3365929..3366339 <gbrowseImage>
name=NC_012967:3365929..3366339
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|3365929|3366339|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3365929|3366339|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 3323203..3323613 <gbrowseImage>
name=NC_012759:3323203..3323613
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|3323203|3323613|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3323203|3323613|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4202392..4201982 <gbrowseImage>
name=NC_007779:4201982..4202392
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|NC_007779|4200982|4203392|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|4201982|4202392|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 3533791..3534201 <gbrowseImage>
name=NC_010473:3533791..3534201
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|3532971|3535021|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|3532971|3535021|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.S46a9f239962db&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="T46a9f23a56149" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
3436046
|
Edman degradation
|
PMID:7511799 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.T46a9f23a56149&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a9f23a9ebac" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔmscL (Keio:JW3252)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] [http://cgsc2.biology.yale.edu/Strain.php?ID=108734 CGSC10453]<ref name='LIB:cgsc'/>
|

|-
|
''ΔmscL727::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:101954|CGSC:101954]]
|

|-
|
mscL-K31C
|
-->TGC
|
K31C
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See Table 1
|-
|
''mscL-K31R''
|
-->AGG
|
K31R
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental:AW405
|
See table 1
|-
|
''mscL-K31E''
|
-->GAG
|
K31E
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental:AW405
|
See table 1
|-
|
''mscL-Q56P''
|
-->CCT
|
Q56P
|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental:AW405
|
see table 1
|-
|
''mscL-Q56C''
|
-->TGC
|
Q56C
|
Conductance
|
Increased channel open time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56F''
|
-->TTT
|
Q56F
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56W''
|
-->TGG
|
Q56W
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
see table 1
|-
|
''mscL-Q56Y''
|
-->TAC
|
Q56Y
|
Conductance
|
Increased open channel time
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56L''
|
-->TTA
|
Q56L
|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-Q56R''
|
-->CGT
|
Q56R
|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscLΔ2-4''
|
Deletion
|

|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscL-ΔQ56''
|
Deletion
|

|
Sensitivity to
|
Pressure
|
PMID:8876191
|
Parental: AW405
|
See table 1
|-
|
''mscLΔ110-136''
|
Deletion
|

|

|
No significant change in pressure sensitivity or open time.
|
PMID:8876191
|
Parental: AW405
|
See table 1

|-
|
''mscL-Q56H''
|
-->CAT
|
Q56H
|
Sensitivity to
|
Increased sensitivity to pressure at pH 5 and increased open channel time at pH 7.5.
|
PMID:8876191
|
Parental: AW405
|
See table 1

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.U46a9f23a9ebac&page=17129&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J4e04bfa3717ad" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.17129.J4e04bfa3717ad&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9f23b5eb85" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW3252
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCAGCATTATTAAAGAATTTCG

Primer 2:CCAGAGCGGTTATTCTGCTCTTT
|-
|
[[smg:Gene|smg-3082::Tn10]]
|
Linked marker
|
CAG12071 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40240 CGSC7444]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 78% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
|-
|
[[yhfT:Gene|zhf-3084::Tn10]]
|
Linked marker
|
CAG18456 = [http://cgsc2.biology.yale.edu/Strain.php?ID=61450 CGSC7576]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 2% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.B46a9f23b5eb85&page=17129&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="N46a9f23c0d3d3" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[[EcoCyc]]
|
EcoCyc:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG11180
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[RegulonDB]]
|
RegulonDB:ECK120001162
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947787 GeneID:947787]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EchoBASE]]
|
EchoBASE:EB1167
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[ASAP]]
|
ASAP:ABE-0010790
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.N46a9f23c0d3d3&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P46a70d7f13e9e" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17129.P46a70d7f13e9e&page=17129&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:msc genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

mscL:Gene

2013-11-06T16:33:36Z

Jtownley: Table edited by Jtownley via TableEdit

mscL:Gene

2013-11-05T22:42:18Z

Jtownley: Table edited by Jtownley via TableEdit

mscL:Gene

2013-11-05T22:32:02Z

Jtownley: Table edited by Jtownley via TableEdit

hemB:Gene

2013-10-07T18:02:01Z

Jtownley: Table edited by Jtownley via TableEdit

hemL:Gene

2013-10-07T16:16:08Z

Jtownley: Table edited by Jtownley via TableEdit

Category:Complex:DNA gyrase

2013-10-07T06:05:23Z

Jtownley:

{{Complex placeholder}}
==Effects on Gene Expression==
Inhibition of gyrase by a quinolone causes a decreased expression of enzymes in the anabolic pathway of cysteine, sulfate reductase and O-actylserine sulfhydrylase both of which are products of the cysB gene. <ref name='PMID:6264273'/>

Quinolones

2013-10-01T16:04:22Z

Jtownley: /* Usage Examples and Notes */

==Targets==
Type II Topoisomerases
*DNA Gyrase
*Topoisomerase IV<ref name='PMID:8524852'/>
**inhibition by norfloxacin reduces activity to a rate of 1/100 the normal level<ref name='PMID:7590259'/>

Results in replication fork collapse and induction of the SOS response due to permanent double-stranded breaks caused by the formation of poisonous protein:DNA adducts.

==Resistant Alleles==
*ParC

==Usage Examples and Notes==
*Norfloxacin<ref name='PMID:16164551'/>
*Oxolinic acid and nalidixic acid interact with the same target to inhibit replication DNA synthesis. Although nalidixic acid is used extensively, oxolinic acid has ben found to be 10-fold more active of an inhibitor. Mutants with resistance to one drug also show resistance to the other drug. <ref name='PMID:770163'/>

== References ==
{{RefHelp}}<references/>

[[Category:Antibiotics]]

gyrB:Gene

2013-10-01T05:21:51Z

Jtownley: Table edited by Jtownley via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|gyrB}}{{Tab On|Gene|gyrB}}{{Tab|Gene Product(s)|gyrB}}{{Tab|Expression|gyrB}}{{Tab|Evolution|gyrB}}{{Tab|On One Page|gyrB}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="U46a9fc9fa216f" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''gyrB''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Gyrase
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK3691, b3699, JW5625, Cou, himB, hisU, nalC, nalD, pcbA, parA, hopA, pcpA''<ref name='LIB:Riley_2006'/>, acrB, pcpA''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.U46a9fc9fa216f&page=17957&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J46a9fca030db8" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
83.53 minutes, 83.53 minutes 
|
MG1655: 3878142..3875728 <gbrowseImage>
name=NC_000913:3875728..3878142
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10424 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|3874728|3879142|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|3875728|3878142|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10424 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 3840480..3838066 <gbrowseImage>
name=NC_012967:3838066..3840480
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|3838066|3840480|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3838066|3840480|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 3764061..3766475 <gbrowseImage>
name=NC_012759:3764061..3766475
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|3764061|3766475|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3764061|3766475|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 3760296..3762710 <gbrowseImage>
name=NC_007779:3760296..3762710
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|NC_007779|3759296|3763710|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|3760296|3762710|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.J46a9fca030db8&page=17957&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a9fca0e9109" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
3875731
|
Edman degradation
|
PMID:3029692 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.U46a9fca0e9109&page=17957&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="K46a9fca13ef43" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
gyrBD426N
|

|
D426N
|

|
(in nal-24, nal-102, nal-103, nal- 107, nal-108, nal-111, nal-114, en-2 and en-5 mutants; resistant to nalidixic acid and to enoxacin)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES6 UniProt:P0AES6]
|-
|
gyrBK447E
|

|
K447E
|

|
(in nal-31, nal-109, nal-115 and nal-120 mutants; resistant to nalidixic acid)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES6 UniProt:P0AES6]
|-
|
gyrBW751R
|

|
W751R
|

|
(in B17 resistant mutant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES6 UniProt:P0AES6]
|-
|
gyrBSR759RC
|

|
SR759RC
|

|
(in acriflavine susceptible mutant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES6 UniProt:P0AES6]
|-
|
''gyrB41''(Const)
|

|

|

|

|

|
[[cgscmutation:6633|CGSC:6633]]
|

|-
|
''gyrB226''
|

|

|

|

|

|
[[cgscmutation:5052|CGSC:5052]]
|

|-
|
''gyrB225''
|

|

|

|

|

|
[[cgscmutation:7206|CGSC:7206]]
|

|-
|
''gyrB204''
|

|

|

|

|

|
[[cgscmutation:9714|CGSC:9714]]
|

|-
|
''gyrB203''(ts)
|

|

|

|
temperature sensitive
|

|
[[cgscmutation:12298|CGSC:12298]]
|

|-
|
''gyrB221''(CouR)
|

|

|

|
coumermycin resistant
|

|
[[cgscmutation:12299|CGSC:12299]]
|

|-
|
gyrB234(ts)
|

|

|
Cell Shape
|
at permissive temperature (30°C), the average cell volume is larger than the gyrB+ parent (LE234)
|
PMID:94144
|

|
Strain LE316
|-
|
''nalD'' mutation in strain AB1157-81
|

|

|
Resistant to
|
Mutant is resistant to Nalidixic acid (NAL)
|
PMID:3906038
|
Strain: AB1157-81
|
Resistance occurs at 37C but sensitive at 30C. Refer to Figure 3 in the Paper for experimental results.
|-
|
gyrB234(ts)
|

|

|
Resistant to
|
Resistant to chlorobiocin (50 ug/ml) (at permissive growth temperatures)
|
PMID:94144
|

|
Strain LE316, parental strain LE234
|-
|
gyrB234(ts)
|

|

|
Growth Phenotype
|
Temperature-sensitive growth, the plating efficiency at 42°C is less than 10-7 that of the gyrB{ parental strain LE234
|
PMID:94144
|

|
Strain LE316, parental strain is LE234
|-
|
gyrB234(ts)
|

|

|
Resistant to
|
Coumermycin A1 resistant (at permissive growth temperatures)
|
PMID:94144
|

|
Strain LE316, parental strain LE234
|-
|
Multicopy ''gyrB'' in strain RYC1010
|

|

|
Resistant to
|
Increased resistance towards coumermycin A1
|
PMID:1656457
|

|
Plasmid pCID549 was used to increase gene dosage of gyrB causing increased resistance toward coumermycin A1.
|-
|
''gyrB-225''
|
6 base pair insertion between nucloetide 1134 and 1135 of gyrB.
see figure 1.
|
2 amino acid insertion between 378 and 379 in the 43kDa N terminal region of gyr B. See fig 1.
|
Sensitivity to
|
Increase (2x) sensitivity to quinolone Ciprofloxacin Figure 2.
|
PMID:11399091
|
Experimental Strain: SD104-20

parental: SD104
|
See figure 2a/b.

Ciprofloxacin 100241

|-
|
''gyrB-225''
|
6 base pair insertion between nucloetide 1134 and 1135 of gyrB.
see figure 1.
|
2 amino acid insertion between 378 and 379 in the 43kDa N terminal region of gyr B. See fig 1.
|
Sensitivity to
|
Increase (2x) sensitivity to quinolone oxolinic acid Figure 2.
|
PMID:11399091
|
Experimental Strain: SD104-20

parental: SD104
|
See figure 2a/b.

CHEBI ID:

oxolinic acid 138856

|-
|
''gyrB-225''
|

|

|
DNA relaxation
|
Decreased DNA relaxation by 2 times.
|
PMID:11399091
|

|
See table 1.
|-
|
''gyrB-225''
|

|

|
DNA cleavage
|
DNA cleavage is reduced in mutant. Figure 5.
|
PMID:11399091
|

|
See Figure 5.
|-
|
gyrB-NI741
|

|

|
Resistant to
|
Resistant to coumermycin at 60 µg/ml.
|
PMID:794878
|
From strain NI708.

Parental strain: K12
|

|-
|
gyrB-NI748
|

|

|
Resistant to
|
Resistant to coumermycin at four times the regular lethal limit (60 µg/ml).
|
PMID:794878
|
From strain NI741 and and CRT46 dnaA
|
Used as a source for drug-resistant DNA gyrase.
|-
|
gyrB-NT525
|

|

|
Resistant to
|
Novobiocin at 600 µg/ml.
|
PMID:794878
|
Parental strain: K12
|
Resistance due to drug impermeability.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.K46a9fca13ef43&page=17957&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S4e04bcc9ad9c2" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.17957.S4e04bcc9ad9c2&page=17957&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="O46a9fca203146" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW5625
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:cloned as old JW

Primer 1:GCCTCGAATTCTTATGACTCCTC

Primer 2:CCAATATCGATATTCGCCGCTTT
|-
|
[[:Category:Kohara_Phage:3C10|3C10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=565 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3D1|3D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=566 Genobase]
|
PMID:3038334
|-
|
zid-501::Tn10
|
Linked marker
|
CAG18499 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38945 CGSC7458]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 95% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[rbsA:Gene|rbsD296::Tn10]]
|
Linked marker
|
CAG18501 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40333 CGSC7460]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 7% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zie-296::Tn10

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.O46a9fca203146&page=17957&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="H46a9fca2a22e5" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[[EcoCyc]]
|
EcoCyc:EG10424
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG10424
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[RegulonDB]]
|
RegulonDB:ECK120000417
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948211 GeneID:948211]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EchoBASE]]
|
EchoBASE:EB0419
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[ASAP]]
|
ASAP:ABE-0012093
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.H46a9fca2a22e5&page=17957&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S46a716dc9e48a" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.S46a716dc9e48a&page=17957&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes not in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:gyr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

gyrB:Gene

2013-10-01T05:17:54Z

Jtownley: Table edited by Jtownley via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|gyrB}}{{Tab On|Gene|gyrB}}{{Tab|Gene Product(s)|gyrB}}{{Tab|Expression|gyrB}}{{Tab|Evolution|gyrB}}{{Tab|On One Page|gyrB}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="U46a9fc9fa216f" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''gyrB''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Gyrase
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK3691, b3699, JW5625, Cou, himB, hisU, nalC, nalD, pcbA, parA, hopA, pcpA''<ref name='LIB:Riley_2006'/>, acrB, pcpA''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.U46a9fc9fa216f&page=17957&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="J46a9fca030db8" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
83.53 minutes, 83.53 minutes 
|
MG1655: 3878142..3875728 <gbrowseImage>
name=NC_000913:3875728..3878142
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10424 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|3874728|3879142|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|3875728|3878142|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10424 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 3840480..3838066 <gbrowseImage>
name=NC_012967:3838066..3840480
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|3838066|3840480|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3838066|3840480|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 3764061..3766475 <gbrowseImage>
name=NC_012759:3764061..3766475
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|3764061|3766475|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3764061|3766475|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 3760296..3762710 <gbrowseImage>
name=NC_007779:3760296..3762710
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|NC_007779|3759296|3763710|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|3760296|3762710|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.J46a9fca030db8&page=17957&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="U46a9fca0e9109" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
3875731
|
Edman degradation
|
PMID:3029692 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.U46a9fca0e9109&page=17957&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="K46a9fca13ef43" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
gyrBD426N
|

|
D426N
|

|
(in nal-24, nal-102, nal-103, nal- 107, nal-108, nal-111, nal-114, en-2 and en-5 mutants; resistant to nalidixic acid and to enoxacin)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES6 UniProt:P0AES6]
|-
|
gyrBK447E
|

|
K447E
|

|
(in nal-31, nal-109, nal-115 and nal-120 mutants; resistant to nalidixic acid)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES6 UniProt:P0AES6]
|-
|
gyrBW751R
|

|
W751R
|

|
(in B17 resistant mutant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES6 UniProt:P0AES6]
|-
|
gyrBSR759RC
|

|
SR759RC
|

|
(in acriflavine susceptible mutant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES6 UniProt:P0AES6]
|-
|
''gyrB41''(Const)
|

|

|

|

|

|
[[cgscmutation:6633|CGSC:6633]]
|

|-
|
''gyrB226''
|

|

|

|

|

|
[[cgscmutation:5052|CGSC:5052]]
|

|-
|
''gyrB225''
|

|

|

|

|

|
[[cgscmutation:7206|CGSC:7206]]
|

|-
|
''gyrB204''
|

|

|

|

|

|
[[cgscmutation:9714|CGSC:9714]]
|

|-
|
''gyrB203''(ts)
|

|

|

|
temperature sensitive
|

|
[[cgscmutation:12298|CGSC:12298]]
|

|-
|
''gyrB221''(CouR)
|

|

|

|
coumermycin resistant
|

|
[[cgscmutation:12299|CGSC:12299]]
|

|-
|
gyrB234(ts)
|

|

|
Cell Shape
|
at permissive temperature (30°C), the average cell volume is larger than the gyrB+ parent (LE234)
|
PMID:94144
|

|
Strain LE316
|-
|
''nalD'' mutation in strain AB1157-81
|

|

|
Resistant to
|
Mutant is resistant to Nalidixic acid (NAL)
|
PMID:3906038
|
Strain: AB1157-81
|
Resistance occurs at 37C but sensitive at 30C. Refer to Figure 3 in the Paper for experimental results.
|-
|
gyrB234(ts)
|

|

|
Resistant to
|
Resistant to chlorobiocin (50 ug/ml) (at permissive growth temperatures)
|
PMID:94144
|

|
Strain LE316, parental strain LE234
|-
|
gyrB234(ts)
|

|

|
Growth Phenotype
|
Temperature-sensitive growth, the plating efficiency at 42°C is less than 10-7 that of the gyrB{ parental strain LE234
|
PMID:94144
|

|
Strain LE316, parental strain is LE234
|-
|
gyrB234(ts)
|

|

|
Resistant to
|
Coumermycin A1 resistant (at permissive growth temperatures)
|
PMID:94144
|

|
Strain LE316, parental strain LE234
|-
|
Multicopy ''gyrB'' in strain RYC1010
|

|

|
Resistant to
|
Increased resistance towards coumermycin A1
|
PMID:1656457
|

|
Plasmid pCID549 was used to increase gene dosage of gyrB causing increased resistance toward coumermycin A1.
|-
|
''gyrB-225''
|
6 base pair insertion between nucloetide 1134 and 1135 of gyrB.
see figure 1.
|
2 amino acid insertion between 378 and 379 in the 43kDa N terminal region of gyr B. See fig 1.
|
Sensitivity to
|
Increase (2x) sensitivity to quinolone Ciprofloxacin Figure 2.
|
PMID:11399091
|
Experimental Strain: SD104-20

parental: SD104
|
See figure 2a/b.

Ciprofloxacin 100241

|-
|
''gyrB-225''
|
6 base pair insertion between nucloetide 1134 and 1135 of gyrB.
see figure 1.
|
2 amino acid insertion between 378 and 379 in the 43kDa N terminal region of gyr B. See fig 1.
|
Sensitivity to
|
Increase (2x) sensitivity to quinolone oxolinic acid Figure 2.
|
PMID:11399091
|
Experimental Strain: SD104-20

parental: SD104
|
See figure 2a/b.

CHEBI ID:

oxolinic acid 138856

|-
|
''gyrB-225''
|

|

|
DNA relaxation
|
Decreased DNA relaxation by 2 times.
|
PMID:11399091
|

|
See table 1.
|-
|
''gyrB-225''
|

|

|
DNA cleavage
|
DNA cleavage is reduced in mutant. Figure 5.
|
PMID:11399091
|

|
See Figure 5.
|-
|
gyrB-NI741
|

|

|
Resistant to
|
Resistant to coumermycin at 60 µg/ml.
|
PMID:794878
|
From strain NI708.

Parental strain: K12
|

|-
|
gyrB-NI748
|

|

|
Resistant to
|
Resistant to coumermycin at four times the regular lethal limit (60 µg/ml).
|
PMID:794878
|
From strain NI741 and and CRT46 dnaA
|
Used as a source for drug-resistant DNA gyrase.
|-
|
gyrB-N99 recB
|

|

|
Resistant to
|

|

|

|

|-
|

|

|

|

|

|

|

|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17957.K46a9fca13ef43&page=17957&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S4e04bcc9ad9c2" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.17957.S4e04bcc9ad9c2&page=17957&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="O46a9fca203146" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW5625
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:cloned as old JW

Primer 1:GCCTCGAATTCTTATGACTCCTC

Primer 2:CCAATATCGATATTCGCCGCTTT
|-
|
[[:Category:Kohara_Phage:3C10|3C10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=565 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3D1|3D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=566 Genobase]
|
PMID:3038334
|-
|
zid-501::Tn10
|
Linked marker
|
CAG18499 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38945 CGSC7458]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 95% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[rbsA:Gene|rbsD296::Tn10]]
|
Linked marker
|
CAG18501 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40333 CGSC7460]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 7% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zie-296::Tn10

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<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="H46a9fca2a22e5" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[[EcoCyc]]
|
EcoCyc:EG10424
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[EcoGene]]
|
EcoGene:EG10424
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[RegulonDB]]
|
RegulonDB:ECK120000417
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948211 GeneID:948211]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[EchoBASE]]
|
EchoBASE:EB0419
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|
[[ASAP]]
|
ASAP:ABE-0012093
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]

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|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S46a716dc9e48a" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

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|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes not in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:gyr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

gyrA:Gene

2013-09-18T01:44:26Z

Jtownley: Table edited by Jtownley via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|gyrA}}{{Tab On|Gene|gyrA}}{{Tab|Gene Product(s)|gyrA}}{{Tab|Expression|gyrA}}{{Tab|Evolution|gyrA}}{{Tab|On One Page|gyrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="R46a9d91c5e712" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''gyrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Gyrase
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK2223, b2231, JW2225, hisW, nalA, parD, nfxA, norA''<ref name='LIB:Riley_2006'/>, norA''

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|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C46a9d91cdc05f" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
50.32 minutes 
|
MG1655: 2337442..2334815 <gbrowseImage>
name=NC_000913:2334815..2337442
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10423 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|2333815|2338442|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|2334815|2337442|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10423 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 2286120..2283493 <gbrowseImage>
name=NC_012967:2283493..2286120
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|2283493|2286120|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|2283493|2286120|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 2220620..2223247 <gbrowseImage>
name=NC_012759:2220620..2223247
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|2220620|2223247|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|2220620|2223247|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 2344090..2341463 <gbrowseImage>
name=AC_000091:2341463..2344090
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|2340463|2345090|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|2341463|2344090|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 2428430..2425803 <gbrowseImage>
name=NC_010473:2425803..2428430
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|2420549|2433684|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|2420549|2433684|FASTA Dump}}

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|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="G46a9d91d92aa0" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
2334818
|
Edman degradation
|
PMID:3029031 
|

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|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="M46a9d91dd79d1" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
gyrAA67S
|

|
A67S
|
Resistant to
|
(in PPA-10; quinolone-resistant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAG81C
|

|
G81C
|

|
(in NAL-97; quinolone-resistant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAD678E
|

|
D678E
|

|
(in strain: 227)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAI798IMMI
|

|
I798IMMI
|

|
(in KL-16; quinolone- resistant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAA828S
|

|
A828S
|

|
(in strain: 227)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAS83A
|

|
S83A
|

|
Resistant to fluoroquinolones
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAQ106R
|

|
Q106R
|

|
Resistant to fluoroquinolones
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAS83W
|

|
S83W
|

|
(in PPA-18 and strain 227; quinolone-resistant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAA84P
|

|
A84P
|

|
(in PPA-05; quinolone-resistant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAD87N
|
GAC -> AAC at codon 87
|
D87N
|
Resistant to
|
(in NAL-113 and KL-16; quinolone- resistant)

resistant to 200 ug/ml nalidixic acid
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAQ106H
|

|
Q106H
|

|
(in NAL-89; quinolone-resistant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAD87V
|

|
D87V
|

|
(in strain: 202; quinolone- resistant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
gyrAS83L
|

|
S83L
|

|
(in NAL-51, NAL-112, NAL-118 and NAL-119; quinolone-resistant)
|

|

|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0AES4 UniProt:P0AES4]
|-
|
''gyrA98''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:4735|CGSC:4735]]
|

|-
|
''gyrA20''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:4693|CGSC:4693]]
|

|-
|
''gyrA12''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:5833|CGSC:5833]]
|

|-
|
''gyrA26''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:6591|CGSC:6591]]
|

|-
|
''gyrA39''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:6657|CGSC:6657]]
|

|-
|
''gyrA96''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:6781|CGSC:6781]]
|

|-
|
''gyrA91''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:6799|CGSC:6799]]
|

|-
|
''gyrA93''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:7183|CGSC:7183]]
|

|-
|
''gyrA216''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:7227|CGSC:7227]]
|

|-
|
''gyrA220''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:7261|CGSC:7261]]
|

|-
|
''gyrA218''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:7270|CGSC:7270]]
|

|-
|
''gyrA13''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:8316|CGSC:8316]]
|

|-
|
''gyrA19''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:8423|CGSC:8423]]
|

|-
|
''gyrA111''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:8506|CGSC:8506]]
|

|-
|
''gyrA261''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:8753|CGSC:8753]]
|

|-
|
''gyrA90''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:9163|CGSC:9163]]
|

|-
|
''gyrA99''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:9220|CGSC:9220]]
|

|-
|
''gyrA49''
|

|

|

|

|

|
[[cgscmutation:9554|CGSC:9554]]
|

|-
|
''gyrA217''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:9588|CGSC:9588]]
|

|-
|
''gyrA223''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:9628|CGSC:9628]]
|

|-
|
''gyrA219''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:9719|CGSC:9719]]
|

|-
|
''gyrA271''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:10266|CGSC:10266]]
|

|-
|
''gyrA260''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:10312|CGSC:10312]]
|

|-
|
''gyrA270''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:10450|CGSC:10450]]
|

|-
|
''[gyrA43]''(ts)
|

|

|

|
temperature sensitive
|

|
[[cgscmutation:10853|CGSC:10853]]
|

|-
|
''gyrA25''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:10898|CGSC:10898]]
|

|-
|
''gyrA21''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:11844|CGSC:11844]]
|

|-
|
''gyrA222''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:12272|CGSC:12272]]
|

|-
|
''gyrA29''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:12928|CGSC:12928]]
|

|-
|
''gyrA17''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:14039|CGSC:14039]]
|

|-
|
''gyrA42''(ts)
|

|

|

|
temperature sensitive
|

|
[[cgscmutation:17046|CGSC:17046]]
|

|-
|
''gyrA283''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:17056|CGSC:17056]]
|

|-
|
''gyrA44''(ts)
|

|

|

|
temperature sensitive
|

|
[[cgscmutation:17109|CGSC:17109]]
|

|-
|
''gyrA37''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:19366|CGSC:19366]]
|

|-
|
''gyrA329''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:23665|CGSC:23665]]
|

|-
|
''gyrA284''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:26543|CGSC:26543]]
|

|-
|
''gyrA900''
|

|

|

|

|

|
[[cgscmutation:73380|CGSC:73380]]
|

|-
|
''gyrA586''(NalR)
|

|

|

|
nalidixic acid resistant
|

|
[[cgscmutation:90278|CGSC:90278]]
|

|-
|
gyrA(Am) from OV6
|

|

|
Growth Phenotype
|
at 42C the separation of DNA into nucleoids and division localization are both disturbed but cells have continued growth and DNA replication
production of cells, and normal sized cells are all produced at 42C
|
PMID:330757
|

|

|-
|
''gyrA43(TS)''
|

|

|
Resistant to
|
Resistant to Nalidixic Acid at the permissive temperature 32 C.
|
PMID:3025879
|
Strain: KRF949

Parental Strain: [http://cgsc.biology.yale.edu/Strain.php?ID=10860 CGSC KNK43]

[http://cgsc.biology.yale.edu/Mutation.php?ID=10853 CGSC] of allele, not strain.
|

|-
|
''gyrA43''
|

|

|
Resistant to
|
Resistant to Triazolealanine.
|
PMID:3025879
|
Strain: KRE449

Parental:[http://cgsc.biology.yale.edu/Strain.php?ID=10860 CGSC KNK453]
|
Results on table 4.
|-
|
nalA mutation in MH5
|

|

|
Resistant to
|
resistance to nalidixic acid
|
PMID:4895844
|

|
similar strain: MH7
resistant at 40ug nal per ml, sensitive at 60ug nal per ml
|-
|
''gyrAR'' ''S83L''
|

|
''S83L''
|
Resistant to
|
Quinolone Resistance
|
PMID:8524852
|

|
The mutation conferred an increase in resistance toward Quinolones. See figure 1B.
chEBI:23765
|-
|
gyrAnal-112
|
TCG-->TTG
|
S83L
|
Resistant to
|
Resistant to nalidixic acid
|
PMID:2168148
|
Parental strain: KL16
|
Results found in Table 1.
Mutation and phenotype identical in strains gyrAnal-118, gyrAnal-119, and gyrAnal-51.
|-
|
gyrAppa-18
|
TCG-->TGG
|
S83W
|
Resistant to
|
Resistant to pipemidic acid.
|
PMID:2168148
|
Parental strain: KL16
|
Results found in Table 1.
|-
|
gyrAnal-113
|
GAC-->AAC
|
D87N
|
Resistant to
|
Resistant to nalidixic acid
|
PMID:2168148
|
Parental strain: KL16
|
Results found in Table 1
|-
|
gyrAnal-97
|
GGT-->TGT
|
G81C
|
Resistant to
|
Resistant to nalidixic acid.
|
PMID:2168148
|
Parental strain: KL16
|
Results found in Table 1.
|-
|
gyrAppa-5
|
GCG-->CCG
|
A84P
|
Resistant to
|
Resistant to pipemidic acid.
|
PMID 2168148
|
Parental strain: KL16
|
Results found in Table 1.
|-
|
gyrAppa-10
|
GGC-->TCC
|
A67S
|
Resistant to
|
Resistant to pipemidic acid.
|
PMID:2168148
|
Parental strain: KL16
|
Results found in Table 1.
|-
|
gyrAnal-89
|
CAG-->CAT
|
Q106H
|
Resistant to
|
Resistant to nalidixic acid.
|
PMID:2168148
|
Parental strain: KL16
|
Results found in Table 1.

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===Notes===
A Latin American study found that the ''gyrA'' single-point mutation has caused ''E. coli'' to become resistant to Fluoroquinolones, or antimicrobial drugs. This has resulted in an increase in the number of urinary tract infections. <ref name='PMID:10744370'/>

==Genetic Interactions==
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{{Genetic_interaction_help}}
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

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===Notes===

==Genetic Resources==
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{{Gene_resources_help}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="B46a9d91e8e70c" class=" tableEdit Gene_resource_table"
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!|Resource!!Resource Type!!Source!!Notes/Reference
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JW2225
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Plasmid clone
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
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PMID:16769691

Status:Clone OK

Primer 1:GCCAGCGACCTTGCGAGAGAAAT

Primer 2:CCTTCTTCTTCTGGCTCGTCGTC
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[[:Category:Kohara_Phage:E13A5|E13A5]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=376 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:2E8|2E8]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=377 Genobase]
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PMID:3038334
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[[atoS:Gene|atoS298::Tn10]]
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Linked marker
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CAG12177 gyrA+ = [http://cgsc2.biology.yale.edu/Strain.php?ID=44154 CGSC7525]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 59% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zeh-298::Tn10, zej-298::Tn10 nnnThe original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).
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[[yfaL:Gene|zfa-723::Tn10]]
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Linked marker
|
CAG12178 gyrA+ = [http://cgsc2.biology.yale.edu/Strain.php?ID=44155 CGSC7526]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 76% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

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===Notes===

==Accessions in Other Databases==
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{{Gene_accessions_help}}
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!|Database!!Accession!!Notes
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[[EcoCyc]]
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EcoCyc:EG10423
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EcoGene]]
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EcoGene:EG10423
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://www.ncbi.nlm.nih.gov/gene?term=946614 GeneID:946614]
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Escherichia coli str. K-12 substr. MG1655
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[[RegulonDB]]
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RegulonDB:ECK120000416
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EchoBASE]]
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EchoBASE:EB0418
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0007370
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]

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===Notes===

==Links==
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!|Name!!URL!!Comments

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<noinclude><protect>
==References==
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{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes not in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:gyr genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]