EcoliWiki - User contributions [en]

nrdI:Gene Product(s)

2010-02-26T23:28:48Z

Jcotruvo: Table edited by Jcotruvo via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|nrdI}}{{Tab|Gene|nrdI}}{{Tab On|Gene Product(s)|nrdI}}{{Tab|Expression|nrdI}}{{Tab|Evolution|nrdI}}{{Tab|On One Page|nrdI}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==

{{Product_nomenclature_help}}
{| id="Z46aab5e602204" class=" tableEdit Product_nomenclature_table"

|-
!align=left {{table heading style}} |Standard name
||
NrdI
|-
!align=left {{table heading style}} |Synonyms
||
protein that stimulates ribonucleotide reduction<ref name='LIB:Riley_2006'/>, B2674<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YgaO<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, NrdI<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
conserved protein that may stimulate ribonucleotide reductase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Part of nrdHIEF operon, function unknown; stimulatory effect on ribonucleotide reduction; non-essential gene<ref name='LIB:EcoGene'/>
|-
!align=left {{table heading style}} |EC number (for enzymes)
||

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.Z46aab5e602204&page=25646&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}


<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> 
{{Product_GO_help}}
{| id="M46aab5e71286f" class=" tableEdit GO_table_product dataTable"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
GO:0015949
|
nucleobase, nucleoside and nucleotide interconversion
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
GO:0006464
|
protein modification process
|
PMID:18799738
|
IDA: Inferred from Direct Assay
|

|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0010181
|
FMN binding
|
PMID:18799738
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.M46aab5e71286f&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>

{{Product_interactions_help}}
{| id="S46aab5ea65462" class=" tableEdit Product_interactions_table dataTable"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.S46aab5ea65462&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || || || ||
|}


</protect>
===Notes===

==Localization==

{{Product_localization_help}}
{| id="E46a84d5c3a369" class=" tableEdit Product_localization_table"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Source!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.E46a84d5c3a369&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table] || || || ||
|}


<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>

{{Product_physical_properties_help}}
{| id="R46aab5ec4ecb8" class=" tableEdit Product_phys_prop_table"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=G7402-MONOMER at EcoCyc]
<pre>MSQLVYFSSS SENTQRFIER LGLPAVRIPL NERERIQVDE PYILIVPSYG GGGTAGAVPR
QVIRFLNDEH NRALLRGVIA SGNRNFGEAY GRAGDVIARK CGVPWLYRFE LMGTQSDIEN
VRKGVTEFWQ RQPQNA</pre>
|-
!align=left {{table heading style}} |Length
||
136
|-
!align=left {{table heading style}} |Mol. Wt
||
15.339 kDa
|-
!align=left {{table heading style}} |pI
||
9.7 (calculated)
|-
!align=left {{table heading style}} |Extinction coefficient
||
18,450 - 18,575 (calc based on 5 Y, 2 W, and 1 C residues)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.R46aab5ec4ecb8&page=25646&pagename={{FULLPAGENAMEE}}&type=1&template=Product_phys_prop_table edit table] ||
|}


<big>'''Domains/Motifs/Modification Sites'''</big><table>
<tr align="left" valign="top">
<td>
{{Product_domains_motifs_help}}
{| id="D46aab5ecbe44e" class=" tableEdit Product_motif_table dataTable"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes!!References

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.D46aab5ecbe44e&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table] || || || ||
|}
</td>
<td>


<motif_map/>
</td>
</tr>
</table>

<table><tr valign='top'><td>
<big>'''Structure'''</big> 

{{Product_structure_help}}
{| id="B46a84d5dddbfe" class=" tableEdit Product_structure_table"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=nrdI
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.B46a84d5dddbfe&page=25646&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}


</td><td></protect>
==== Structure figures ====

<protect>
</td><tr><table></protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}

{{Product_resources_help}}
{| id="T46aab5ed87e37" class=" tableEdit Product_resource_table"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.T46aab5ed87e37&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table] || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="F46aab5ede653a" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G7402-MONOMER EcoCyc:G7402-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0A772|UniProt:P0A772]]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417160 RefSeq:NP_417160]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.F46aab5ede653a&page=25646&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="O46a84d6097dad" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.O46a84d6097dad&page=25646&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0015949_!_nucleobase, nucleoside and nucleotide interconversion]]

nrdI:Gene Product(s)

2010-02-26T23:21:43Z

Jcotruvo: Table edited by Jcotruvo via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|nrdI}}{{Tab|Gene|nrdI}}{{Tab On|Gene Product(s)|nrdI}}{{Tab|Expression|nrdI}}{{Tab|Evolution|nrdI}}{{Tab|On One Page|nrdI}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==

{{Product_nomenclature_help}}
{| id="Z46aab5e602204" class=" tableEdit Product_nomenclature_table"

|-
!align=left {{table heading style}} |Standard name
||
NrdI
||

|-
!align=left {{table heading style}} |Synonyms
||
protein that stimulates ribonucleotide reduction<ref name='LIB:Riley_2006'/>, B2674<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YgaO<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, NrdI<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
||

|-
!align=left {{table heading style}} |Product description
||
conserved protein that may stimulate ribonucleotide reductase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Part of nrdHIEF operon, function unknown; stimulatory effect on ribonucleotide reduction; non-essential gene<ref name='LIB:EcoGene'/>
||
flavodoxin-like protein
|-
!align=left {{table heading style}} |EC number (for enzymes)
||

||

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.Z46aab5e602204&page=25646&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] || ||
|}


<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> 
{{Product_GO_help}}
{| id="M46aab5e71286f" class=" tableEdit GO_table_product dataTable"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
GO:0015949
|
nucleobase, nucleoside and nucleotide interconversion
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
GO:0006464
|
protein modification process
|
PMID:18799738
|
IDA: Inferred from Direct Assay
|

|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0010181
|
FMN binding
|
PMID:18799738
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.M46aab5e71286f&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>

{{Product_interactions_help}}
{| id="S46aab5ea65462" class=" tableEdit Product_interactions_table dataTable"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.S46aab5ea65462&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || || || ||
|}


</protect>
===Notes===

==Localization==

{{Product_localization_help}}
{| id="E46a84d5c3a369" class=" tableEdit Product_localization_table"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Source!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.E46a84d5c3a369&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table] || || || ||
|}


<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>

{{Product_physical_properties_help}}
{| id="R46aab5ec4ecb8" class=" tableEdit Product_phys_prop_table"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=G7402-MONOMER at EcoCyc]
<pre>MSQLVYFSSS SENTQRFIER LGLPAVRIPL NERERIQVDE PYILIVPSYG GGGTAGAVPR
QVIRFLNDEH NRALLRGVIA SGNRNFGEAY GRAGDVIARK CGVPWLYRFE LMGTQSDIEN
VRKGVTEFWQ RQPQNA</pre>
|-
!align=left {{table heading style}} |Length
||
136
|-
!align=left {{table heading style}} |Mol. Wt
||
15.339 kDa
|-
!align=left {{table heading style}} |pI
||
9.7 (calculated)
|-
!align=left {{table heading style}} |Extinction coefficient
||
18,450 - 18,575 (calc based on 5 Y, 2 W, and 1 C residues)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.R46aab5ec4ecb8&page=25646&pagename={{FULLPAGENAMEE}}&type=1&template=Product_phys_prop_table edit table] ||
|}


<big>'''Domains/Motifs/Modification Sites'''</big><table>
<tr align="left" valign="top">
<td>
{{Product_domains_motifs_help}}
{| id="D46aab5ecbe44e" class=" tableEdit Product_motif_table dataTable"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes!!References

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.D46aab5ecbe44e&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table] || || || ||
|}
</td>
<td>


<motif_map/>
</td>
</tr>
</table>

<table><tr valign='top'><td>
<big>'''Structure'''</big> 

{{Product_structure_help}}
{| id="B46a84d5dddbfe" class=" tableEdit Product_structure_table"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=nrdI
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.B46a84d5dddbfe&page=25646&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}


</td><td></protect>
==== Structure figures ====

<protect>
</td><tr><table></protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}

{{Product_resources_help}}
{| id="T46aab5ed87e37" class=" tableEdit Product_resource_table"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.T46aab5ed87e37&page=25646&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table] || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="F46aab5ede653a" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G7402-MONOMER EcoCyc:G7402-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0A772|UniProt:P0A772]]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417160 RefSeq:NP_417160]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.F46aab5ede653a&page=25646&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="O46a84d6097dad" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.25646.O46a84d6097dad&page=25646&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0015949_!_nucleobase, nucleoside and nucleotide interconversion]]

PMID:20070127

2010-02-26T23:00:31Z

Jcotruvo: /* Significance {{HelpIcon|Help:Literature}} */

{{RightTOC}}
<protect>
 '''Cotruvo, JA and Stubbe, J''' (2010) An active dimanganese(III)-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase.''Biochemistry'' '''49''':1297-309

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates and is expressed under iron-limited and oxidative stress conditions. This RNR is composed of two homodimeric subunits: alpha2 (NrdE), where nucleotide reduction occurs, and beta2 (NrdF), which contains an unidentified metallocofactor that initiates nucleotide reduction. nrdE and nrdF are found in an operon with nrdI, which encodes an unusual flavodoxin proposed to be involved in metallocofactor biosynthesis and/or maintenance. Ni affinity chromatography of a mixture of E. coli (His)(6)-NrdI and NrdF demonstrated tight association between these proteins. To explore the function of NrdI and identify the metallocofactor, apoNrdF was loaded with Mn(II) and incubated with fully reduced NrdI (NrdI(hq)) and O(2). Active RNR was rapidly produced with 0.25 +/- 0.03 tyrosyl radical (Y*) per beta2 and a specific activity of 600 units/mg. EPR and biochemical studies of the reconstituted cofactor suggest it is Mn(III)(2)-Y*, which we propose is generated by Mn(II)(2)-NrdF reacting with two equivalents of HO(2)(-), produced by reduction of O(2) by NrdF-bound NrdI(hq). In the absence of NrdI(hq), with a variety of oxidants, no active RNR was generated. By contrast, a similar experiment with apoNrdF loaded with Fe(II) and incubated with O(2) in the presence or absence of NrdI(hq) gave 0.2 and 0.7 Y*/beta2 with specific activities of 80 and 300 units/mg, respectively. Thus NrdI(hq) hinders Fe(III)(2)-Y* cofactor assembly in vitro. We propose that NrdI is an essential player in E. coli class Ib RNR cluster assembly and that the Mn(III)(2)-Y* cofactor, not the diferric-Y* one, is the active metallocofactor in vivo.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20070127 PubMed] Online version:[http://dx.doi.org/10.1021/bi902106n 10.1021/bi902106n]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
This paper describes the first successful in vitro assembly of an active dimanganese(III)-tyrosyl radical cofactor in a class Ib ribonucleotide reductase, NrdEF. NrdF can also form a diferric-tyrosyl radical cofactor that is active in nucleotide reduction. While it is not yet clear whether NrdF contains iron or manganese (or both, depending on the growth conditions) in vivo, the paper demonstrates that NrdI is required for assembly of the manganese cofactor. By contrast, the diferric-tyrosyl radical cofactor does not require NrdI for its formation. The requirement for NrdI for dimanganese(III)-tyrosyl radical cofactor assembly provides a rationale for the universal conservation of nrdI in class Ib RNR systems and suggests that this role for NrdI may be relevant inside the cell.

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="H4b8848f1f3ca5" class=" tableEdit GO_table_reference"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
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<references/>