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https://ecoliwiki.org/colipedia/index.php?title=pET-48b(%2B)&diff=1282825
pET-48b(+)
2011-08-08T04:25:58Z
<p>JBehrendorff: Table edited by JBehrendorff via TableEdit</p>
<hr />
<div>==At a Glance==<br />
[[Image:PET-48b(+).jpg|thumb|440px|right|PET-48b(+).jpg Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB416.pdf Novagen]]]<br />
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.O47027c07602a0--><br />
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* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** <br />
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{| {{Prettytable}} width='30%' align=left id="O47027c07602a0" class=" tableEdit PlasmidTable" <br />
<br />
|-<br />
!align=left {{table heading style}} |Type<br />
||<br />
Cloning, Expression<br />
|-<br />
!align=left {{table heading style}} |Origin of Replication<br />
||<br />
f1 <br /> pBR322<br />
|-<br />
!align=left {{table heading style}} |Copy #<br />
||<br />
~40<br />
|-<br />
!align=left {{table heading style}} |Markers<br />
||<br />
Kanamycin<br />
|-<br />
!align=left {{table heading style}} |Link to Sequence<br />
||<br />
[http://www.emdbiosciences.com/docs/docs/LIT/pET48b-gb.html Novagen]<br />
|-<br />
!align=left {{table heading style}} |Features<br />
||<br />
T7 Promoter <br /> His Tag <br /> N Terminal Trx Tag <br /> MCS <br /> C Terminal S Tag <br /> lacI<br />
|-<br />
!align=left {{table heading style}} |Accession Number<br />
||<br />
<br />
<br />
|- class="tableEdit_footer" <br />
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.O47027c07602a0&page=112706&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||<br />
|}<br />
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.O47027c07602a0--></protect><br />
<BR><br />
<Br clear='all'><br />
<br />
==Notes==<br />
The pET-48b(+) vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein that can be cleaved with the human rhinovirus (HRV) 3C protease. This plasmid contains a strong T7lac promoter, optimized RBS, the coding sequence for the rhinovirus 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro) for N-terminal fusion tag removal and a multiple cloning site that contains restriction enzyme sites. There is also an optional C-terminal S•Tag™ coding sequence that is compatible with purification, detection, and quantification<br />
<br />
==History {{HelpIcon|Help:Plasmid}}==<br />
{{PlasmidHistory}}<br />
<br />
==Sources==<br />
[http://www.emdbiosciences.com/product/71462 Novagen]<br />
{{PlasmidSources}}<br />
<br />
==References{{HelpIcon|Help:Plasmid}}==<br />
{{RefHelp}}<br />
<references/><br />
<br />
==Sequence==<br />
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br />
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br />
121 tgtttagcag cctaggttaa ttaagcctcg agagcagcag aagtagagct gtccatgtgc <br />
181 tggcgttcga atttagcagc agcggtttct ttactaccgc gtggcaccag agcgagctct <br />
241 gcggccgcaa gcttgtcgac ggacgtcggg cgcgccaagg cctgtacaga attcggatcc <br />
301 tggtacccgg gtccctgaaa gaggacttca agagccgcgg agtgatggtg atggtgatga <br />
361 ccagaaccac tagatggagt aggagtagga ggattgttat tagaaccgcc accaccacta <br />
421 gtatggccag aaccagaacc ggccaggtta gcgtcgagga actctttcaa ctgaccttta <br />
481 gacagtgcac ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg <br />
541 ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca <br />
601 acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg <br />
661 cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca <br />
721 tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatatg tatatctcct <br />
781 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta <br />
841 tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac gccggacgca <br />
901 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca <br />
961 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta <br />
1021 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc <br />
1081 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt <br />
1141 cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt <br />
1201 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg <br />
1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac <br />
1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg <br />
1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc <br />
1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct <br />
1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa <br />
1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac <br />
1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg <br />
1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac <br />
1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta <br />
1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc <br />
1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt <br />
1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc <br />
1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt <br />
2041 gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg <br />
2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg <br />
2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa <br />
2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca <br />
2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat <br />
2341 taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt <br />
2401 caacccagtc agctccttcc ggtgggcgcg gggcatgact agcatgatcg tgctcctgtc <br />
2461 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat <br />
2521 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg <br />
2581 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac <br />
2641 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc <br />
2701 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca <br />
2761 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac <br />
2821 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc <br />
2881 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt <br />
2941 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca <br />
3001 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc <br />
3061 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc <br />
3121 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg <br />
3181 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt <br />
3241 aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac <br />
3301 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg <br />
3361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa <br />
3421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc <br />
3481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc <br />
3541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat <br />
3601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc <br />
3661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct <br />
3721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg <br />
3781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc <br />
3841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga <br />
3901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa <br />
3961 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta <br />
4021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc <br />
4081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg <br />
4141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaacaat aaaactgtct <br />
4201 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc <br />
4261 tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc <br />
4321 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca <br />
4381 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc <br />
4441 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact <br />
4501 cctgatgatg catggttact caccactgcg atccccggca aaacagcatt ccaggtatta <br />
4561 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg <br />
4621 ttgcattcga ttcctgtttg taattgtcct tttaacagtg atcgcgtatt tcgtctcgct <br />
4681 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt <br />
4741 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg <br />
4801 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa <br />
4861 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc <br />
4921 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa <br />
4981 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt <br />
5041 ttctaagaat taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat <br />
5101 aggggttccg cgcacatttc cccgaaaagt gccacctgaa attgtaaacg ttaatatttt <br />
5161 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat <br />
5221 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt <br />
5281 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt <br />
5341 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag <br />
5401 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg <br />
5461 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc <br />
5521 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc <br />
5581 gctacagggc gcgtcccatt cgcca<br />
<br />
<br />
<br />
<br />
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Kanamycin Marker Plasmids]][[Category:His Tag]][[Category:T7 Promoter]][[Category:S Tag]][[Category:lacI]][[Category:Trx Tag]][[Category:MCS]]<br />
__NOTOC__</div>
JBehrendorff
https://ecoliwiki.org/colipedia/index.php?title=pET-48b(%2B)&diff=1282824
pET-48b(+)
2011-08-08T03:33:20Z
<p>JBehrendorff: </p>
<hr />
<div>==At a Glance==<br />
[[Image:PET-48b(+).jpg|thumb|440px|right|PET-48b(+).jpg Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB416.pdf Novagen]]]<br />
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.O47027c07602a0--><br />
<!--<br />
******************************************************************************************<br />
*<br />
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br />
*<br />
****************************************************************************************** --><br />
{|{{Prettytable}} width='30%' align=left<br />
|-<br />
!{{table heading style}} |Type<br />
|<br />
Cloning, Expression<br />
|-<br />
!{{table heading style}} |Origin of Replication<br />
|<br />
f1 <br> pBR322<br />
|-<br />
!{{table heading style}} |Copy #<br />
|<br />
~40<br />
|-<br />
!{{table heading style}} |Markers<br />
|<br />
Ampicillin<br />
|-<br />
!{{table heading style}} |Link to Sequence<br />
|<br />
[http://www.emdbiosciences.com/docs/docs/LIT/pET48b-gb.html Novagen]<br />
|-<br />
!{{table heading style}} |Features<br />
|<br />
T7 Promoter <br> His Tag <br> N Terminal Trx Tag <br> MCS <br> C Terminal S Tag <br> lacI<br />
|-<br />
!{{table heading style}} |Accession Number<br />
|<br />
<br />
|-<br />
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.O47027c07602a0&page=0&pagename={{FULLPAGENAMEE}} edit table]<br />
|}<br />
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.O47027c07602a0--></protect><br />
<BR><br />
<Br clear='all'><br />
<br />
==Notes==<br />
The pET-48b(+) vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein that can be cleaved with the human rhinovirus (HRV) 3C protease. This plasmid contains a strong T7lac promoter, optimized RBS, the coding sequence for the rhinovirus 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro) for N-terminal fusion tag removal and a multiple cloning site that contains restriction enzyme sites. There is also an optional C-terminal S•Tag™ coding sequence that is compatible with purification, detection, and quantification<br />
<br />
==History {{HelpIcon|Help:Plasmid}}==<br />
{{PlasmidHistory}}<br />
<br />
==Sources==<br />
[http://www.emdbiosciences.com/product/71462 Novagen]<br />
{{PlasmidSources}}<br />
<br />
==References{{HelpIcon|Help:Plasmid}}==<br />
{{RefHelp}}<br />
<references/><br />
<br />
==Sequence==<br />
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br />
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br />
121 tgtttagcag cctaggttaa ttaagcctcg agagcagcag aagtagagct gtccatgtgc <br />
181 tggcgttcga atttagcagc agcggtttct ttactaccgc gtggcaccag agcgagctct <br />
241 gcggccgcaa gcttgtcgac ggacgtcggg cgcgccaagg cctgtacaga attcggatcc <br />
301 tggtacccgg gtccctgaaa gaggacttca agagccgcgg agtgatggtg atggtgatga <br />
361 ccagaaccac tagatggagt aggagtagga ggattgttat tagaaccgcc accaccacta <br />
421 gtatggccag aaccagaacc ggccaggtta gcgtcgagga actctttcaa ctgaccttta <br />
481 gacagtgcac ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg <br />
541 ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca <br />
601 acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg <br />
661 cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca <br />
721 tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatatg tatatctcct <br />
781 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta <br />
841 tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac gccggacgca <br />
901 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca <br />
961 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta <br />
1021 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc <br />
1081 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt <br />
1141 cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt <br />
1201 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg <br />
1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac <br />
1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg <br />
1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc <br />
1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct <br />
1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa <br />
1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac <br />
1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg <br />
1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac <br />
1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta <br />
1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc <br />
1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt <br />
1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc <br />
1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt <br />
2041 gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg <br />
2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg <br />
2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa <br />
2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca <br />
2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat <br />
2341 taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt <br />
2401 caacccagtc agctccttcc ggtgggcgcg gggcatgact agcatgatcg tgctcctgtc <br />
2461 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat <br />
2521 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg <br />
2581 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac <br />
2641 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc <br />
2701 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca <br />
2761 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac <br />
2821 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc <br />
2881 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt <br />
2941 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca <br />
3001 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc <br />
3061 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc <br />
3121 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg <br />
3181 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt <br />
3241 aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac <br />
3301 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg <br />
3361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa <br />
3421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc <br />
3481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc <br />
3541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat <br />
3601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc <br />
3661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct <br />
3721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg <br />
3781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc <br />
3841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga <br />
3901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa <br />
3961 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta <br />
4021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc <br />
4081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg <br />
4141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaacaat aaaactgtct <br />
4201 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc <br />
4261 tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc <br />
4321 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca <br />
4381 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc <br />
4441 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact <br />
4501 cctgatgatg catggttact caccactgcg atccccggca aaacagcatt ccaggtatta <br />
4561 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg <br />
4621 ttgcattcga ttcctgtttg taattgtcct tttaacagtg atcgcgtatt tcgtctcgct <br />
4681 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt <br />
4741 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg <br />
4801 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa <br />
4861 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc <br />
4921 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa <br />
4981 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt <br />
5041 ttctaagaat taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat <br />
5101 aggggttccg cgcacatttc cccgaaaagt gccacctgaa attgtaaacg ttaatatttt <br />
5161 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat <br />
5221 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt <br />
5281 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt <br />
5341 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag <br />
5401 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg <br />
5461 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc <br />
5521 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc <br />
5581 gctacagggc gcgtcccatt cgcca<br />
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[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Kanamycin Marker Plasmids]][[Category:His Tag]][[Category:T7 Promoter]][[Category:S Tag]][[Category:lacI]][[Category:Trx Tag]][[Category:MCS]]<br />
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JBehrendorff